##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_34211-2.34200000035860.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3527945 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00248473261346 33.0 33.0 33.0 33.0 33.0 2 31.890644553699108 33.0 33.0 33.0 33.0 33.0 3 31.85330780383481 33.0 33.0 33.0 33.0 33.0 4 35.5630833247117 37.0 37.0 37.0 33.0 37.0 5 35.58490169206153 37.0 37.0 37.0 33.0 37.0 6 35.67552413657243 37.0 37.0 37.0 33.0 37.0 7 35.50916638439658 37.0 37.0 37.0 33.0 37.0 8 35.53891429713332 37.0 37.0 37.0 33.0 37.0 9 35.408971228292955 37.0 37.0 37.0 33.0 37.0 10-11 35.35663552011157 37.0 37.0 37.0 33.0 37.0 12-13 35.35027019979053 37.0 37.0 37.0 33.0 37.0 14-15 37.10336725770952 40.0 37.0 40.0 33.0 40.0 16-17 37.055915129062384 40.0 37.0 40.0 33.0 40.0 18-19 37.00164359138252 40.0 37.0 40.0 33.0 40.0 20-21 36.91636788555377 40.0 37.0 40.0 33.0 40.0 22-23 37.11493943357961 40.0 37.0 40.0 33.0 40.0 24-25 36.81222411347116 40.0 37.0 40.0 33.0 40.0 26-27 36.758049657803625 40.0 37.0 40.0 33.0 40.0 28-29 36.686673403355215 40.0 37.0 40.0 33.0 40.0 30-31 36.616291070297294 40.0 37.0 40.0 33.0 40.0 32-33 36.50161751387847 40.0 37.0 40.0 33.0 40.0 34-35 36.520842586831705 40.0 37.0 40.0 33.0 40.0 36-37 36.36147445609271 40.0 37.0 40.0 33.0 40.0 38-39 36.29840558738869 40.0 37.0 40.0 33.0 40.0 40-41 36.35959800960616 40.0 37.0 40.0 33.0 40.0 42-43 36.190428705662924 40.0 37.0 40.0 33.0 40.0 44-45 35.9381591833206 38.5 37.0 40.0 27.0 40.0 46-47 35.83664087733794 37.0 37.0 40.0 27.0 40.0 48-49 35.718863956212466 37.0 37.0 40.0 27.0 40.0 50-51 34.6128671223616 37.0 35.0 38.5 24.5 38.5 52-53 35.009879830893055 37.0 35.0 38.5 27.0 40.0 54-55 35.73584324585559 37.0 37.0 40.0 27.0 40.0 56-57 35.80809919088875 37.0 37.0 40.0 27.0 40.0 58-59 35.60468984068629 37.0 37.0 40.0 27.0 40.0 60-61 35.402677479382476 37.0 37.0 40.0 27.0 40.0 62-63 35.14983255124442 37.0 37.0 40.0 27.0 40.0 64-65 34.96471529459785 37.0 33.0 40.0 27.0 40.0 66-67 34.79004108624142 37.0 33.0 40.0 27.0 40.0 68-69 34.64137621193074 37.0 33.0 40.0 27.0 40.0 70-71 34.27312061270797 37.0 33.0 38.5 27.0 40.0 72-73 34.12801758530816 37.0 33.0 37.0 27.0 40.0 74-75 33.93629520868381 37.0 33.0 37.0 27.0 40.0 76-77 33.638225794336364 37.0 33.0 37.0 27.0 40.0 78-79 33.27391385069778 37.0 33.0 37.0 22.0 37.0 80-81 32.904028265746774 37.0 33.0 37.0 22.0 37.0 82-83 32.58620386655688 37.0 33.0 37.0 22.0 37.0 84-85 32.348364416111934 37.0 33.0 37.0 22.0 37.0 86-87 32.15062366335076 37.0 33.0 37.0 22.0 37.0 88-89 31.943428256392885 35.0 33.0 37.0 22.0 37.0 90-91 31.7343517260048 33.0 33.0 37.0 15.0 37.0 92-93 31.564238954972367 33.0 33.0 37.0 15.0 37.0 94-95 31.408607843943145 33.0 33.0 37.0 15.0 37.0 96-97 31.246692054439624 33.0 33.0 37.0 15.0 37.0 98-99 31.080389150057613 33.0 33.0 37.0 15.0 37.0 100-101 29.7213832698639 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15328046653866068 2306 2 0.1757881101036851 2306 3 0.13918051592699854 2306 4 0.22541727885539586 2306 5 0.20966098694836433 2306 6 0.17163077122956594 2306 7 0.2674862694693161 2306 8 0.18057871887636878 2306 9 0.2052698722323001 2306 10-11 0.13693836149892036 2306 12-13 0.11783551307193108 2306 14-15 0.325365286111051 2306 16-17 0.36825520618776153 2306 18-19 0.36070058980427433 2306 20-21 0.3935112036812072 2306 22-23 0.409467851684056 2306 24-25 0.3961267800926578 2306 26-27 0.31968453190113877 2306 28-29 0.3322405021931871 2306 30-31 0.2623861214911969 2306 32-33 0.24968460541835213 2306 34-35 0.22413831454628763 2306 36-37 0.20012396250257325 2306 38-39 0.12465065267144126 2306 40-41 0.04615794358841896 2306 42-43 0.13182876860620496 2306 44-45 0.14945677556890757 2306 46-47 0.11169729533050798 2306 48-49 0.007906464314423545 2306 50-51 -0.020040493451055852 2306 52-53 0.07967090327617399 2306 54-55 0.2600641138627964 2306 56-57 0.23011668472631897 2306 58-59 0.04291181543476341 2306 60-61 0.23441771428082347 2306 62-63 0.35195861855631705 2306 64-65 0.2764708413867538 2306 66-67 0.15266532574226233 2306 68-69 0.15099276606154177 2306 70-71 0.19001522107584634 2306 72-73 0.07333363683054728 2306 74-75 0.11123323346343028 2306 76-77 0.2497371294533508 2306 78-79 0.3248798082134243 2306 80-81 0.30167063195869304 2306 82-83 0.034824921786047014 2306 84-85 0.22467632297128404 2306 86-87 0.29850370400919957 2306 88-89 0.35683855226468353 2306 90-91 0.328752611911284 2306 92-93 0.267617852077894 2306 94-95 0.02640041316314168 2306 96-97 0.048587971394940865 2306 98-99 0.024186923760954215 2306 100-101 -0.23163711455377367 2307 1 0.25328432184748806 2307 2 0.14676870076555915 2307 3 0.18550490732366498 2307 4 0.2818536455552376 2307 5 0.21505877628572279 2307 6 0.20124132792188476 2307 7 0.34237421563206993 2307 8 0.2844378830168566 2307 9 0.288095827884824 2307 10-11 0.3737308944796567 2307 12-13 0.32626475316201464 2307 14-15 0.5015236279284423 2307 16-17 0.47449288169355697 2307 18-19 0.559470674892367 2307 20-21 0.6956926302311572 2307 22-23 0.46624280731229817 2307 24-25 0.38817574108693265 2307 26-27 0.36250005680144426 2307 28-29 0.2891167310728733 2307 30-31 0.29587519026276965 2307 32-33 0.38641241918694647 2307 34-35 0.4250434553864295 2307 36-37 0.30432653470750637 2307 38-39 0.1338039768757895 2307 40-41 -0.005710596091994091 2307 42-43 0.16136793069053113 2307 44-45 0.36606551741028426 2307 46-47 0.24420907901055244 2307 48-49 0.28027475197186646 2307 50-51 0.02970530604718391 2307 52-53 0.06452917783985157 2307 54-55 0.3219944741342644 2307 56-57 0.37634908417058455 2307 58-59 0.4197275588025562 2307 60-61 0.38743688516096597 2307 62-63 0.3735963608874684 2307 64-65 0.2878600662269406 2307 66-67 0.022591132465635155 2307 68-69 0.08421114217690473 2307 70-71 0.04405215779024019 2307 72-73 0.02647075160309953 2307 74-75 0.017798931495654813 2307 76-77 0.16203831331873886 2307 78-79 0.1917925839929424 2307 80-81 0.27308601578324954 2307 82-83 0.12674101741483668 2307 84-85 0.10217424181846013 2307 86-87 0.18453649126897176 2307 88-89 0.32252005663475103 2307 90-91 0.37373228586638163 2307 92-93 0.31816576999694135 2307 94-95 0.2232521251344437 2307 96-97 0.3147060180955812 2307 98-99 0.1503076406199355 2307 100-101 -0.06574496039282707 2201 1 0.27527918265758444 2201 2 0.23708813503273163 2201 3 0.2691109758996397 2201 4 0.320797354970189 2201 5 0.30077558357454137 2201 6 0.263978755260851 2201 7 0.3933758232111799 2201 8 0.4155554388215137 2201 9 0.340577378699372 2201 10-11 0.2826092529205724 2201 12-13 0.3722637728622402 2201 14-15 0.551575174537696 2201 16-17 0.4585324199822125 2201 18-19 0.5286478419244744 2201 20-21 0.7079764403764841 2201 22-23 0.6284055919262386 2201 24-25 0.6018000443638343 2201 26-27 0.4497138606036515 2201 28-29 0.3952753202288264 2201 30-31 0.5332659613803514 2201 32-33 0.5132568103110842 2201 34-35 0.3231246275830486 2201 36-37 0.4754394773367636 2201 38-39 0.49381748588483987 2201 40-41 0.3521146433219613 2201 42-43 0.4278424520384263 2201 44-45 0.4938543436806313 2201 46-47 0.580981958883747 2201 48-49 0.5672923200126903 2201 50-51 0.4111894194244101 2201 52-53 0.522499000921762 2201 54-55 0.6092449864358258 2201 56-57 0.6099781131393556 2201 58-59 0.7166420054957712 2201 60-61 0.5972873156067919 2201 62-63 0.5552818084287665 2201 64-65 0.5795379259385882 2201 66-67 0.5667063802761376 2201 68-69 0.5622681444286357 2201 70-71 0.5553997957178538 2201 72-73 0.7348967938220881 2201 74-75 0.557207298783176 2201 76-77 0.5104636804388889 2201 78-79 0.6680581130243084 2201 80-81 0.7643104341082463 2201 82-83 0.6496664246189852 2201 84-85 0.5888674108027274 2201 86-87 0.6385000246800345 2201 88-89 0.7294188283768008 2201 90-91 0.6103582965807703 2201 92-93 0.48122868639579863 2201 94-95 0.4391427563096606 2201 96-97 0.48079615504219575 2201 98-99 0.43822780174352616 2201 100-101 0.34600520521735945 1101 1 0.15505003470764223 1101 2 0.13476030287849383 1101 3 0.14624405154566844 1101 4 0.09770802305435922 1101 5 0.20272944410341154 1101 6 0.2070974217495447 1101 7 0.2950766036256809 1101 8 0.1734450220503163 1101 9 0.09074659841424904 1101 10-11 0.21574277287682975 1101 12-13 0.24421683186556464 1101 14-15 0.3886332349657735 1101 16-17 0.40377583918922255 1101 18-19 0.4292069217731509 1101 20-21 0.4914336630525895 1101 22-23 0.44339992602417055 1101 24-25 0.3439655265713881 1101 26-27 0.27806203635990556 1101 28-29 0.3100356814454628 1101 30-31 0.32623538318065215 1101 32-33 0.2133986866445241 1101 34-35 0.2109035561074819 1101 36-37 0.339770154591136 1101 38-39 0.36129399313943367 1101 40-41 0.08121123567080701 1101 42-43 0.17495388333900763 1101 44-45 0.23557701457834668 1101 46-47 0.2066214042911767 1101 48-49 0.32641495878626614 1101 50-51 0.3177448728433063 1101 52-53 0.25303660367244873 1101 54-55 0.5124692176260908 1101 56-57 0.48912439060541146 1101 58-59 0.4702198388333585 1101 60-61 0.49272925438980764 1101 62-63 0.41628883692425944 1101 64-65 0.36656856409492633 1101 66-67 0.3505422764400805 1101 68-69 0.36392922723916143 1101 70-71 0.3773760800284336 1101 72-73 0.3566437913357845 1101 74-75 0.3839743549470782 1101 76-77 0.366439084964739 1101 78-79 0.325716216305608 1101 80-81 0.2813315707156576 1101 82-83 0.2786773263131366 1101 84-85 0.38159811052506853 1101 86-87 0.44425496234565287 1101 88-89 0.4367902916058668 1101 90-91 0.4665654019469798 1101 92-93 0.4205805772545226 1101 94-95 0.40441650410744145 1101 96-97 0.40050601541581443 1101 98-99 0.3835321242698342 1101 100-101 0.3188142398618794 2304 1 0.17986615900242953 2304 2 0.10602384180440438 2304 3 0.042616894627080626 2304 4 0.1655333667340031 2304 5 0.16029658905470257 2304 6 0.18398417973818226 2304 7 0.23995232540521982 2304 8 0.06887984711342199 2304 9 0.06271361690217958 2304 10-11 0.21252632308014796 2304 12-13 0.15822039380802977 2304 14-15 0.21733860984840447 2304 16-17 0.1698777801681004 2304 18-19 0.2731069119678935 2304 20-21 0.34488671556992045 2304 22-23 0.29418294766717423 2304 24-25 0.13490701852931153 2304 26-27 0.22900873243690256 2304 28-29 0.04922599819867912 2304 30-31 0.05904420323899018 2304 32-33 0.009411478564778974 2304 34-35 -0.07403377853068349 2304 36-37 0.10645721944896991 2304 38-39 -0.02665033636713332 2304 40-41 0.009128414668872153 2304 42-43 0.01292576805852974 2304 44-45 0.14793045520453774 2304 46-47 0.14087214016067406 2304 48-49 0.04980930742802059 2304 50-51 0.01613120208966734 2304 52-53 0.08144917895358361 2304 54-55 0.25558088971008885 2304 56-57 0.23740378552607666 2304 58-59 0.24679350767483754 2304 60-61 0.28110345336696696 2304 62-63 0.17351939814070505 2304 64-65 0.03147666766341217 2304 66-67 -0.018198215381765692 2304 68-69 0.10220780460249301 2304 70-71 0.136349043326085 2304 72-73 0.05476522929146199 2304 74-75 0.19064839321039528 2304 76-77 0.18612783464253368 2304 78-79 0.2477603595123199 2304 80-81 0.22168682517833105 2304 82-83 0.03204642302636529 2304 84-85 0.14040593267457524 2304 86-87 0.2365863696660142 2304 88-89 0.14883166163638606 2304 90-91 -0.08401416743117807 2304 92-93 0.1332480169338659 2304 94-95 0.06287846691320098 2304 96-97 -0.0026549980184746858 2304 98-99 -0.02555692673623966 2304 100-101 -0.08388462280994702 2202 1 0.1149001837257444 2202 2 0.06191519763968145 2202 3 0.11055107818732068 2202 4 0.16186669867467884 2202 5 0.130858795106235 2202 6 0.14290973134814067 2202 7 0.2165969315237959 2202 8 0.13558902266791506 2202 9 0.1291284907038701 2202 10-11 0.19861537350662672 2202 12-13 0.22861380950980248 2202 14-15 0.30892574540882833 2202 16-17 0.34763327402610145 2202 18-19 0.3014673403139554 2202 20-21 0.44100720405921834 2202 22-23 0.2770179668969348 2202 24-25 0.2097637445716103 2202 26-27 0.26197003722464984 2202 28-29 0.24181331734441613 2202 30-31 0.254469783234633 2202 32-33 0.17304890568669862 2202 34-35 -0.0754416345565474 2202 36-37 0.10583064548755772 2202 38-39 0.06399785986418038 2202 40-41 -0.04536846696136365 2202 42-43 0.05519038748274596 2202 44-45 0.23101884428152886 2202 46-47 0.30249615007182484 2202 48-49 0.1967180453760804 2202 50-51 0.08128930295738712 2202 52-53 0.11111080198044476 2202 54-55 0.2667035298585603 2202 56-57 0.24259725912972385 2202 58-59 0.08276396493988614 2202 60-61 0.23700119905240058 2202 62-63 0.19952866883024 2202 64-65 0.26984707892154347 2202 66-67 0.25262710503849917 2202 68-69 0.1974165720667571 2202 70-71 0.2502537750838627 2202 72-73 0.30043911640689913 2202 74-75 0.12768219527200841 2202 76-77 0.21199081462780356 2202 78-79 0.3631975493741777 2202 80-81 0.34628841187643644 2202 82-83 0.34707973749326015 2202 84-85 0.3182953061817102 2202 86-87 0.38701456335613926 2202 88-89 0.41856259733410184 2202 90-91 0.3754389769034958 2202 92-93 0.3502234894940095 2202 94-95 0.33270420586865157 2202 96-97 0.3349177141755497 2202 98-99 0.2840469811978181 2202 100-101 0.1197278298283635 1102 1 -0.07025660521382804 1102 2 -0.005792951513914346 1102 3 0.04130121117992047 1102 4 0.06354654928065884 1102 5 0.07693698873228527 1102 6 0.05093118577205047 1102 7 0.20577118625889312 1102 8 0.11184709323024578 1102 9 0.16079665854375946 1102 10-11 0.1917511052210088 1102 12-13 0.17952500724020837 1102 14-15 0.20571078962893807 1102 16-17 0.11343465036127753 1102 18-19 0.1839887573499439 1102 20-21 0.27486307413187916 1102 22-23 0.021882273938992114 1102 24-25 -0.04464061226480709 1102 26-27 -0.006916198077057345 1102 28-29 -0.08937870469814158 1102 30-31 -0.10206560823328203 1102 32-33 -0.10125699009768852 1102 34-35 -0.05581210228431388 1102 36-37 -0.008017915603147685 1102 38-39 -0.026870324792064082 1102 40-41 -0.0695156967466275 1102 42-43 0.04935907672805939 1102 44-45 0.0278191283202176 1102 46-47 -0.04274042676274803 1102 48-49 -0.0768697615428593 1102 50-51 -0.1519486209370129 1102 52-53 -0.042758650361570005 1102 54-55 0.18459539436824457 1102 56-57 0.2776186797238651 1102 58-59 0.2794743866000502 1102 60-61 0.2977540460902759 1102 62-63 0.2308653815641506 1102 64-65 0.1749888973669158 1102 66-67 0.2154242117063916 1102 68-69 0.1469761664905107 1102 70-71 0.08203135869019462 1102 72-73 0.09799131908071956 1102 74-75 0.12989279007708632 1102 76-77 0.14471577411145375 1102 78-79 0.06177090747805636 1102 80-81 0.19824649317889254 1102 82-83 0.24314307995989992 1102 84-85 0.24802693319546876 1102 86-87 0.17290009370675108 1102 88-89 0.18906893921162293 1102 90-91 0.22623316085445566 1102 92-93 0.2669220061407138 1102 94-95 0.2753028598817444 1102 96-97 0.2541359465164348 1102 98-99 0.1954734731043466 1102 100-101 0.21190574113458638 2305 1 0.08016912772087892 2305 2 0.05725962788840633 2305 3 0.06067950255887666 2305 4 0.09390070412861462 2305 5 0.12072811581833776 2305 6 0.19138210774938358 2305 7 0.1940412932103186 2305 8 0.16319550913929248 2305 9 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21.476262580670436 26.941129246996454 18.29935054870374 6 31.441247701401764 21.909845280462335 28.43123703823329 18.21766997990261 7 33.940160395518184 22.738408438810904 23.050329598489114 20.271101567181795 8 29.40613053995303 28.288649802508797 22.725531773641777 19.579687883896398 9 29.804586460213823 24.287232965044474 25.104924448439135 20.803256126302568 10-11 25.96924099836769 29.73932273010995 27.86018903807241 16.431247233449948 12-13 26.428261270154753 28.162112681191132 27.35154643811176 18.058079610542354 14-15 24.94110547220459 28.186454272793384 28.16708993931374 18.70535031568829 16-17 25.51363081336629 28.031016157475815 28.200855195517033 18.25449783364087 18-19 24.961197800012425 28.07090582533719 28.602888171648278 18.365008203002102 20-21 24.342214352695237 29.060583017339486 28.052877888005057 18.544324741960224 22-23 25.36649997559788 28.61049223047859 27.507516420356588 18.51549137356694 24-25 23.404129615451307 30.034525929041045 27.818742004525443 18.742602450982208 26-27 23.183902921091263 30.098918937127056 27.837813137768606 18.87936500401307 28-29 23.482290139052367 29.807168515235173 27.73349396120099 18.977047384511472 30-31 24.01749683695836 28.572717144743937 28.336620074628886 19.07316594366881 32-33 22.813569301918218 29.46994310507445 28.562298394716823 19.15418919829051 34-35 24.199497311531605 28.640939730800834 27.99536067300928 19.164202284658284 36-37 22.861344464392598 29.086152858660768 28.67094021526103 19.381562461685604 38-39 22.703261011503393 29.908564817610618 27.99747586663357 19.39069830425242 40-41 24.075355803987495 28.419034016027787 27.960514677265273 19.54509550271944 42-43 23.78564213511915 28.41892249710377 28.195894215918155 19.599541151858922 44-45 22.639561652213416 29.102920817100692 28.576186743136823 19.681330787549076 46-47 23.101846719706206 29.166958014627088 27.877181911981648 19.854013353685055 48-49 21.808663999839712 29.017310123573907 28.73522858892304 20.43879728766334 50-51 21.44520544440901 28.42187063872266 29.31676566677411 20.816158250094226 52-53 23.470724109492515 28.391057128600565 28.00430848196502 20.133910279941897 54-55 23.263263652973706 28.359926733816444 28.527448319448744 19.849361293761106 56-57 23.52532569731377 28.245906356915228 28.32045702429718 19.908310921473817 58-59 23.004423389256836 28.530576518382 27.979095116367965 20.4859049759932 60-61 21.851352004068655 28.35344457735134 27.944234981915507 21.8509684366645 62-63 22.753819797603285 28.45202311481837 28.242182838895168 20.551974248683173 64-65 21.801062786025565 29.063358200994088 28.856789803231596 20.278789209748744 66-67 21.452174939591917 28.92692316052078 28.258410357871277 21.362491542016027 68-69 21.26732323864454 28.47939083516518 29.962324988211908 20.29096093797837 70-71 21.18529308026224 29.781252516953344 28.781062311632454 20.252392091151957 72-73 21.18061695783955 30.756915986335276 27.86805541055739 20.194411645267778 74-75 20.9928774165908 30.902701967876283 27.971421026486702 20.132999589046214 76-77 21.17129703483796 30.81900816361725 27.805292565012827 20.204402236531962 78-79 21.09670242345234 30.89259284355728 27.855503435398777 20.155201297591603 80-81 21.12241758035686 30.716342204716042 27.862031715129255 20.299208499797842 82-83 21.238727418779792 30.691077099105495 27.70232527472465 20.36787020739006 84-85 21.223168068385103 30.479525532873247 27.87637880641508 20.420927592326567 86-87 21.464311260135673 30.10435413737825 27.850130282819862 20.581204319666213 88-89 21.501443113288335 30.18251585804818 27.79528692399973 20.52075410466376 90-91 21.568361127919506 29.915758812164157 27.837796251776485 20.678083808139853 92-93 21.627885075258018 29.92239609737583 27.73352655355178 20.716192273814375 94-95 21.565985986204954 29.828198980605713 27.8794096018743 20.72640543131503 96-97 21.725345350873308 29.61168551225105 27.822122000302258 20.840847136573387 98-99 21.672560148319683 29.744816269703144 27.77193717723776 20.81068640473941 100-101 21.896031929496182 29.6063357731893 27.71843938065916 20.779192916655358 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 63.0 1 106.0 2 166.0 3 166.5 4 147.5 5 171.0 6 254.0 7 387.0 8 518.0 9 704.5 10 920.0 11 1058.5 12 1141.0 13 1169.5 14 1124.5 15 1079.0 16 1041.5 17 1032.5 18 1162.0 19 1482.0 20 2064.5 21 2965.0 22 4287.0 23 6192.0 24 8663.0 25 11871.5 26 16190.5 27 21232.5 28 27164.0 29 34055.0 30 41642.0 31 50594.0 32 60932.5 33 72367.5 34 84356.0 35 96801.5 36 110381.5 37 124362.5 38 136735.5 39 147284.0 40 156310.5 41 163145.5 42 167151.0 43 168164.0 44 167300.5 45 165256.0 46 161854.0 47 157256.0 48 150655.0 49 141763.0 50 131507.5 51 120282.5 52 107356.0 53 92509.0 54 77712.0 55 64443.0 56 53038.0 57 43564.0 58 35514.5 59 28634.0 60 22888.5 61 18016.0 62 13899.0 63 10686.5 64 8234.5 65 6164.0 66 4692.0 67 3560.0 68 2679.5 69 2071.5 70 1571.0 71 1228.0 72 980.5 73 774.5 74 619.0 75 492.5 76 374.0 77 296.5 78 238.0 79 192.5 80 152.0 81 133.5 82 107.0 83 83.0 84 74.0 85 60.0 86 58.5 87 47.0 88 29.5 89 24.0 90 19.0 91 18.0 92 17.5 93 15.0 94 10.5 95 5.5 96 4.0 97 4.0 98 1.5 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1880698253515857 2 0.17744040794286758 3 0.15119283322160634 4 0.14563719105598302 5 0.14963385200166104 6 0.1304442104397886 7 0.10365807856981897 8 0.0915830603935152 9 0.09257513935166223 10-11 0.07286111319762638 12-13 0.09207909987258872 14-15 0.09705366721986879 16-17 0.0889186197630632 18-19 0.08548886107918349 20-21 0.08377398173724364 22-23 0.10377145902217863 24-25 0.44790947704683604 26-27 0.28527939069344904 28-29 0.12110449567666164 30-31 0.22660500659732508 32-33 0.25516270803541435 34-35 0.24005476275848972 36-37 0.24216647368368838 38-39 0.25832318814493993 40-41 0.2584649137103895 42-43 0.28360702902114404 44-45 0.2603923814005037 46-47 0.27820728497751523 48-49 0.2630851671440456 50-51 0.2768467195491993 52-53 0.2768892372188342 54-55 0.25497846480032993 56-57 0.27019979052961424 58-59 0.2593577847727218 60-61 0.23682341986623942 62-63 0.23030404385555894 64-65 0.22805060736491076 66-67 0.23644076083952553 68-69 0.2410326691600918 70-71 0.22858916451361913 72-73 0.22068087796153288 74-75 0.22915606677541742 76-77 0.23017649084665437 78-79 0.23224568410221816 80-81 0.2388359228956234 82-83 0.26319854759640526 84-85 0.2565232734637303 86-87 0.25686341482080927 88-89 0.25483673923488037 90-91 0.24673003689116468 92-93 0.22547120207372845 94-95 0.22430905243704197 96-97 0.21987304223847026 98-99 0.2415003635260754 100-101 0.24990468955723516 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3527945.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.14020948934915 #Duplication Level Percentage of deduplicated Percentage of total 1 97.70054525705369 92.95250342979725 2 1.712783551949907 3.2590917188485133 3 0.27924382316413693 0.7970194750332827 4 0.10811159766116564 0.41143040198846115 5 0.05247731684243585 0.24963514589141483 6 0.03721366187277984 0.2124309351865247 7 0.015027599148279122 0.10008102517624683 8 0.01151856664725794 0.08767030750697204 9 0.007321674634847454 0.06269270927150168 >10 0.07404943390830059 1.2069903354058709 >50 0.0016778285051450466 0.09282676387837002 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 2.968861207612551E-5 0.5676277520155962 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 20096 0.5696233926549309 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.003911625606408263 0.0 0.0 2 0.004932049677645201 0.0 5.66902261798299E-5 3 0.007086278272478738 0.0 8.503533926974485E-5 4 0.009807409129110572 0.0 1.4172556544957474E-4 5 0.012670265551191983 0.0 1.700706785394897E-4 6 0.022109188210133662 0.0 1.9841579162940465E-4 7 0.031122934172726616 0.0 1.9841579162940465E-4 8 0.03925798162953221 0.0 1.9841579162940465E-4 9 0.04367981927155894 0.0 1.9841579162940465E-4 10-11 0.053132914487045574 2.834511308991495E-5 1.9841579162940465E-4 12-13 0.06543469356806866 2.834511308991495E-5 2.125883481743621E-4 14-15 0.07663101323858507 2.834511308991495E-5 2.267609047193196E-4 16-17 0.08959890247722116 2.834511308991495E-5 2.267609047193196E-4 18-19 0.10613827596518653 2.834511308991495E-5 2.4093346126427708E-4 20-21 0.12488856827416528 2.834511308991495E-5 2.834511308991495E-4 22-23 0.14719617227592835 2.834511308991495E-5 2.834511308991495E-4 24-25 0.16912111725097756 2.834511308991495E-5 2.834511308991495E-4 26-27 0.18958628890189616 2.834511308991495E-5 2.9762368744410696E-4 28-29 0.21281510907908147 2.834511308991495E-5 3.2596880053402193E-4 30-31 0.2421948187967783 2.834511308991495E-5 3.401413570789794E-4 32-33 0.27612391916540646 2.834511308991495E-5 3.401413570789794E-4 34-35 0.31627477185727104 2.834511308991495E-5 3.543139136239369E-4 36-37 0.363596938160884 2.834511308991495E-5 3.968315832588093E-4 38-39 0.4183738692071447 2.834511308991495E-5 3.968315832588093E-4 40-41 0.47743091232998247 2.834511308991495E-5 4.2517669634872427E-4 42-43 0.5453316307368737 2.834511308991495E-5 4.535218094386392E-4 44-45 0.618575402961214 2.834511308991495E-5 4.535218094386392E-4 46-47 0.6984802767616842 2.834511308991495E-5 4.960394790735116E-4 48-49 0.7818999445853039 2.834511308991495E-5 5.243845921634266E-4 50-51 0.8717822981934242 2.834511308991495E-5 5.385571487083841E-4 52-53 0.9647259240152553 2.834511308991495E-5 5.66902261798299E-4 54-55 1.0647133104399304 2.834511308991495E-5 5.66902261798299E-4 56-57 1.1699728878993296 2.834511308991495E-5 5.810748183432564E-4 58-59 1.2778685608760907 2.834511308991495E-5 6.094199314331714E-4 60-61 1.3872948699597074 2.834511308991495E-5 6.235924879781289E-4 62-63 1.5011713617984408 5.66902261798299E-5 6.235924879781289E-4 64-65 1.6209436371598764 5.66902261798299E-5 6.377650445230864E-4 66-67 1.7451377501633387 5.66902261798299E-5 6.519376010680439E-4 68-69 1.8738670812611875 5.66902261798299E-5 6.519376010680439E-4 70-71 2.0037160443260875 5.66902261798299E-5 6.802827141579588E-4 72-73 2.139106477000067 5.66902261798299E-5 7.086278272478738E-4 74-75 2.2808036973365517 5.66902261798299E-5 7.511454968827461E-4 76-77 2.427815626377395 5.66902261798299E-5 7.653180534277036E-4 78-79 2.5806099584885818 7.086278272478737E-5 7.653180534277036E-4 80-81 2.7365364255962037 8.503533926974485E-5 8.078357230625761E-4 82-83 2.901434120996784 8.503533926974485E-5 8.220082796075336E-4 84-85 3.0766636101186386 8.503533926974485E-5 8.503533926974485E-4 86-87 3.2620123046135925 8.503533926974485E-5 8.786985057873634E-4 88-89 3.454333896928665 8.503533926974485E-5 9.353887319671933E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 1630 0.0 71.90156 2 GATCGGG 1880 0.0 64.61719 1 GAGCGGC 1165 0.0 63.495674 9 GTTCGGA 315 0.0 58.74664 2 TCGGGAG 1835 0.0 56.623928 3 AAGCGGC 465 0.0 49.96761 9 GATCCGG 460 0.0 49.516434 1 AGAGCGG 1605 0.0 48.15684 8 CGGGAGA 2110 0.0 47.89284 4 GGTTCGG 505 0.0 46.983418 1 GAGAGCG 2030 0.0 46.01476 7 TTCGGAG 385 0.0 45.596653 3 GGATCGG 865 0.0 45.533173 1 TCGGAGA 550 0.0 44.85534 4 TCGGAAA 790 0.0 43.839867 4 TTCGGAA 385 0.0 41.899628 3 CGGAAAG 1090 0.0 40.47844 5 AGCGGCG 1130 0.0 40.284523 10-11 ATCCGGA 560 0.0 36.43421 2 GGCGCCG 1290 0.0 34.978966 56-57 >>END_MODULE