FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_34211-2.34200000035860.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_34211-2.34200000035860.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3527945
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT200960.5696233926549309Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA16300.071.901562
GATCGGG18800.064.617191
GAGCGGC11650.063.4956749
GTTCGGA3150.058.746642
TCGGGAG18350.056.6239283
AAGCGGC4650.049.967619
GATCCGG4600.049.5164341
AGAGCGG16050.048.156848
CGGGAGA21100.047.892844
GGTTCGG5050.046.9834181
GAGAGCG20300.046.014767
TTCGGAG3850.045.5966533
GGATCGG8650.045.5331731
TCGGAGA5500.044.855344
TCGGAAA7900.043.8398674
TTCGGAA3850.041.8996283
CGGAAAG10900.040.478445
AGCGGCG11300.040.28452310-11
ATCCGGA5600.036.434212
GGCGCCG12900.034.97896656-57
Produced by FastQC (version 0.11.2)