FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_34211-1.34200000035897.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_34211-1.34200000035897.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3843546
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT72550.18875798546446432Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA9600.062.2895362
GATCGGG11900.052.653551
GAGCGGC7450.050.9152379
TCGGGAG12600.041.0459063
GTTCGGA2200.038.8298382
TCGTGCG500.001623210137.966952
AGCGGCG6000.036.35088710-11
AGAGCGG11400.033.690358
GTCGCCG10850.033.2713656-57
TCGCCGT9650.032.9787556-57
CGCCGTA10800.032.7609858-59
GGTTCGG3650.032.512311
GCCGTAT11200.032.43902258-59
TTGTGTT327850.032.1134531
CGACGCC753.091989E-431.6453151
GTGTTTG727800.031.3713861
TGTTTGA739100.030.7765562
CGAGGGG12000.030.4586141
CGGGAGA18800.030.2840984
TTTGACG21200.029.9888744
Produced by FastQC (version 0.11.2)