##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_34139-1.342000000356e9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2792336 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.285837377736776 33.0 33.0 33.0 27.0 33.0 2 30.837887345935446 33.0 33.0 33.0 27.0 33.0 3 30.71434813002447 33.0 33.0 33.0 27.0 33.0 4 34.170673586559786 37.0 37.0 37.0 27.0 37.0 5 34.2392434148326 37.0 37.0 37.0 27.0 37.0 6 34.72401602099461 37.0 37.0 37.0 27.0 37.0 7 34.26084396720165 37.0 37.0 37.0 27.0 37.0 8 34.419510402759556 37.0 37.0 37.0 27.0 37.0 9 33.97242129886948 37.0 37.0 37.0 27.0 37.0 10-11 33.97351357429765 37.0 37.0 37.0 27.0 37.0 12-13 33.98233808538801 37.0 37.0 37.0 27.0 37.0 14-15 35.616935605170724 40.0 37.0 40.0 27.0 40.0 16-17 35.5285850270168 40.0 37.0 40.0 24.5 40.0 18-19 35.54151631465554 40.0 37.0 40.0 24.5 40.0 20-21 35.44438008176667 40.0 37.0 40.0 22.0 40.0 22-23 36.1772684232843 40.0 37.0 40.0 27.0 40.0 24-25 35.59198624377582 40.0 37.0 40.0 22.0 40.0 26-27 35.44259018255683 40.0 37.0 40.0 24.5 40.0 28-29 35.239683548111685 40.0 37.0 40.0 22.0 40.0 30-31 35.20123634834776 40.0 37.0 40.0 18.5 40.0 32-33 35.00950942150229 40.0 37.0 40.0 22.0 40.0 34-35 35.21525185364512 40.0 37.0 40.0 22.0 40.0 36-37 34.91473626383071 40.0 37.0 40.0 18.5 40.0 38-39 34.85123423542153 38.5 37.0 40.0 18.5 40.0 40-41 35.267558954223276 37.0 37.0 40.0 22.0 40.0 42-43 35.01779370390956 37.0 37.0 40.0 22.0 40.0 44-45 34.553349775958196 37.0 37.0 40.0 15.0 40.0 46-47 34.41458048028604 37.0 37.0 40.0 15.0 40.0 48-49 34.303220314460724 37.0 37.0 40.0 15.0 40.0 50-51 33.28339282951622 37.0 33.0 38.5 15.0 38.5 52-53 33.70543731126913 37.0 33.0 38.5 18.5 40.0 54-55 34.41185928197753 37.0 37.0 40.0 18.5 40.0 56-57 34.65824349218718 37.0 35.0 40.0 22.0 40.0 58-59 34.19837548203368 37.0 33.0 40.0 15.0 40.0 60-61 33.93923331576143 37.0 33.0 40.0 15.0 40.0 62-63 33.59162436039216 37.0 33.0 40.0 15.0 40.0 64-65 33.372054437574846 37.0 33.0 40.0 15.0 40.0 66-67 33.24480560362363 37.0 33.0 37.0 15.0 40.0 68-69 33.281953711874216 37.0 33.0 37.0 18.5 40.0 70-71 32.85610345603108 37.0 33.0 37.0 15.0 40.0 72-73 32.98619023641854 37.0 33.0 37.0 22.0 38.5 74-75 33.03613193397929 37.0 33.0 37.0 22.0 37.0 76-77 32.79001864388813 37.0 33.0 37.0 22.0 37.0 78-79 32.38908748803868 37.0 33.0 37.0 22.0 37.0 80-81 31.959206019619415 37.0 33.0 37.0 18.5 37.0 82-83 31.612054745560705 37.0 33.0 37.0 15.0 37.0 84-85 31.37416628944368 37.0 33.0 37.0 15.0 37.0 86-87 31.209105028907697 33.0 33.0 37.0 15.0 37.0 88-89 31.05607097426671 33.0 33.0 37.0 15.0 37.0 90-91 30.891000939714992 33.0 33.0 37.0 15.0 37.0 92-93 30.751496059213505 33.0 33.0 37.0 15.0 37.0 94-95 30.632672070982863 33.0 33.0 37.0 15.0 37.0 96-97 30.500441028586817 33.0 27.0 37.0 15.0 37.0 98-99 30.364061667363814 33.0 27.0 37.0 15.0 37.0 100-101 29.14271008216776 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.032246206198369265 2306 2 0.10905001761173239 2306 3 -0.021931681926712798 2306 4 0.20782085996980726 2306 5 0.07814650962815506 2306 6 -0.3087122760532708 2306 7 0.2563719056549587 2306 8 -0.08063775333537393 2306 9 0.15911702630507563 2306 10-11 0.17135683271957447 2306 12-13 0.09051546716100489 2306 14-15 0.43525899661769785 2306 16-17 0.3885523428980804 2306 18-19 0.262601287230531 2306 20-21 0.3055397499680126 2306 22-23 0.11124544189945595 2306 24-25 0.29934612202445265 2306 26-27 0.043181012223143966 2306 28-29 0.15621698418623708 2306 30-31 0.22644352474856788 2306 32-33 0.08861534801634718 2306 34-35 -0.05018672763463172 2306 36-37 0.22105213531656887 2306 38-39 0.005709686820935644 2306 40-41 -0.2367890102404857 2306 42-43 0.08423574462995731 2306 44-45 0.1967056058365486 2306 46-47 0.20729204981942928 2306 48-49 0.013859129866823139 2306 50-51 -0.09903319307544223 2306 52-53 0.00658259536832162 2306 54-55 0.17879605326150738 2306 56-57 0.08893092605415376 2306 58-59 0.22455542538771311 2306 60-61 0.2615211887222486 2306 62-63 0.27708453375359454 2306 64-65 0.12575785117046223 2306 66-67 0.13711419169392514 2306 68-69 0.0034505915438813872 2306 70-71 0.09499935422570616 2306 72-73 -0.06567739477053891 2306 74-75 -0.18290181864049515 2306 76-77 -0.11641582317106725 2306 78-79 0.10134333628759862 2306 80-81 0.19815722521046553 2306 82-83 0.1282189629351116 2306 84-85 0.16469107384861204 2306 86-87 0.23849881807614537 2306 88-89 0.3756306407373806 2306 90-91 0.36687524117379766 2306 92-93 0.23410817377473236 2306 94-95 0.12309031483583155 2306 96-97 0.13009566921682847 2306 98-99 0.04515008085748207 2306 100-101 -0.03009565327738528 2307 1 0.027293728869981493 2307 2 0.0261912130010451 2307 3 -0.0725942557875996 2307 4 0.03328310614412544 2307 5 -0.06246341985598747 2307 6 -0.022940146419642815 2307 7 0.2828532792719187 2307 8 -0.21496104083333023 2307 9 0.09668713725611866 2307 10-11 0.035267977866176636 2307 12-13 0.03403237562543637 2307 14-15 0.35459534585260855 2307 16-17 0.2445806133429116 2307 18-19 0.39211760767107506 2307 20-21 0.435320595688232 2307 22-23 0.10780009628372511 2307 24-25 0.31015415914858835 2307 26-27 0.07934052823161863 2307 28-29 0.14499955252585295 2307 30-31 0.159164682198373 2307 32-33 0.17363789676656438 2307 34-35 -0.059713270118294304 2307 36-37 0.16308488175135238 2307 38-39 -0.03498286942060247 2307 40-41 -0.26839956749140015 2307 42-43 0.012304633166522194 2307 44-45 0.14846243402887183 2307 46-47 0.05931387336867999 2307 48-49 0.05104683298826984 2307 50-51 -0.07634363336259753 2307 52-53 -0.09492139537371713 2307 54-55 0.04620467986249821 2307 56-57 -0.035911651490430074 2307 58-59 0.2759537769550562 2307 60-61 0.19677478043281837 2307 62-63 0.2624358587944826 2307 64-65 0.28813513430353055 2307 66-67 -0.05489334150742309 2307 68-69 0.0025387485462644577 2307 70-71 -0.004268885091462948 2307 72-73 -0.17734285276892336 2307 74-75 -0.1387803694863976 2307 76-77 -0.11877475619831301 2307 78-79 0.024680576959866585 2307 80-81 0.08832303854450174 2307 82-83 0.03860087504690668 2307 84-85 0.0014942537928988031 2307 86-87 0.12465433842814022 2307 88-89 0.25622333120733387 2307 90-91 0.145863728982345 2307 92-93 0.007599428842485878 2307 94-95 0.09900462573893165 2307 96-97 0.19098124771738156 2307 98-99 0.05278627184729601 2307 100-101 -0.07325280308800686 2201 1 -0.002137482441046501 2201 2 -0.10342963071470024 2201 3 -0.11985431223298093 2201 4 0.033276144151081155 2201 5 -0.03321608706718848 2201 6 -0.1940126967348874 2201 7 0.17758896822972048 2201 8 -0.0778968027282545 2201 9 -0.0617659816041396 2201 10-11 0.0574444852758873 2201 12-13 0.08545611344168691 2201 14-15 0.25646144665940795 2201 16-17 0.19497579859233127 2201 18-19 0.24196553931412978 2201 20-21 0.3252169828028997 2201 22-23 0.13309537181380904 2201 24-25 0.26032174865473223 2201 26-27 -0.11439146656982047 2201 28-29 0.12773585741528137 2201 30-31 0.25710835516979813 2201 32-33 0.08986894402026735 2201 34-35 -0.034181286824619406 2201 36-37 0.12904380722097386 2201 38-39 0.10035581823142792 2201 40-41 -0.10228221572707241 2201 42-43 0.2519481054073154 2201 44-45 0.333145783132494 2201 46-47 0.3094843910071958 2201 48-49 0.1392584587385457 2201 50-51 0.16699604289243553 2201 52-53 0.12560178202257788 2201 54-55 0.2756395155051976 2201 56-57 0.33178713747087585 2201 58-59 0.5220042280614905 2201 60-61 0.4144300262229166 2201 62-63 0.38548898351101 2201 64-65 0.303342686673588 2201 66-67 0.29328422371570184 2201 68-69 0.13814680389983636 2201 70-71 0.34934998960883235 2201 72-73 0.1455176295047167 2201 74-75 0.20758615610435527 2201 76-77 0.20749392881840123 2201 78-79 0.32069213304927047 2201 80-81 0.2771579879134123 2201 82-83 0.2938821098751383 2201 84-85 0.22391344808963254 2201 86-87 0.2541374780167125 2201 88-89 0.4298173515924617 2201 90-91 0.300072694022699 2201 92-93 0.20225337581134895 2201 94-95 0.23029808828412257 2201 96-97 0.37162899564572527 2201 98-99 0.410145579694138 2201 100-101 0.3003918350818573 1101 1 -0.3532039028093621 1101 2 -0.3282294701945929 1101 3 -0.42495374490673044 1101 4 -0.38366504614396035 1101 5 -0.5222204226621159 1101 6 -0.5123809048848287 1101 7 -0.033238157451535244 1101 8 -0.5043134462690375 1101 9 -0.4142127492708667 1101 10-11 -0.3649473308591169 1101 12-13 -0.3820145456330337 1101 14-15 -0.21290264021804006 1101 16-17 -0.2641874644740341 1101 18-19 -0.2792594944324165 1101 20-21 -0.21878821562456352 1101 22-23 -0.3294344162323881 1101 24-25 -0.17584314732683737 1101 26-27 -0.43632518698916556 1101 28-29 -0.3900289776691537 1101 30-31 -0.30085255907055597 1101 32-33 -0.4178470329408057 1101 34-35 -0.4658054111050163 1101 36-37 -0.32565923981578493 1101 38-39 -0.38457925500587464 1101 40-41 -0.6738136196904918 1101 42-43 -0.29028274349796135 1101 44-45 -0.2781140706183223 1101 46-47 -0.2967748216239343 1101 48-49 -0.23290939045828196 1101 50-51 -0.2254316500781215 1101 52-53 -0.27549525319614787 1101 54-55 -0.17749805399716223 1101 56-57 -0.09447236708218298 1101 58-59 -0.040185489413509856 1101 60-61 -0.08500830120485148 1101 62-63 -0.23525315963198068 1101 64-65 -0.15937352493359924 1101 66-67 -0.3403294593671973 1101 68-69 -0.38854345178312855 1101 70-71 -0.22267740449279216 1101 72-73 -0.4572763959250139 1101 74-75 -0.17114540985725313 1101 76-77 -0.024924518726002987 1101 78-79 0.07933376119024871 1101 80-81 -0.03055368412302073 1101 82-83 -0.060016415106172616 1101 84-85 -0.0142971554755853 1101 86-87 0.073770280877401 1101 88-89 0.07514255446896456 1101 90-91 0.020147555369018022 1101 92-93 0.11259482100226492 1101 94-95 -0.06140517640074705 1101 96-97 -0.05837145147520317 1101 98-99 -0.0529368276492832 1101 100-101 -0.04526747059476932 2304 1 -0.08118853867738096 2304 2 -0.16303857522331455 2304 3 -0.10803642044468731 2304 4 -0.07712092672046111 2304 5 -0.11903143199067046 2304 6 -0.0872878328096931 2304 7 0.27248172513980506 2304 8 -0.05607786908979051 2304 9 -0.17405601685610606 2304 10-11 0.03845744074570234 2304 12-13 -0.017893983662709445 2304 14-15 0.13901074425626092 2304 16-17 0.08822880209881845 2304 18-19 0.18115090965047642 2304 20-21 0.14289041441802652 2304 22-23 0.04760238864770372 2304 24-25 0.016906232716671354 2304 26-27 -0.03444296167879202 2304 28-29 0.039566452937911833 2304 30-31 0.1012201293182784 2304 32-33 -0.05831462889960903 2304 34-35 -0.1676592076083807 2304 36-37 0.08322039380671953 2304 38-39 -0.09438658116570053 2304 40-41 -0.13046912112798026 2304 42-43 -0.07493643330227684 2304 44-45 0.19415667289592875 2304 46-47 0.035211756272488515 2304 48-49 0.008987241207968566 2304 50-51 -0.0640515059616078 2304 52-53 -0.08565817111036012 2304 54-55 0.07863068712959631 2304 56-57 0.057945008181043534 2304 58-59 0.08790961834953492 2304 60-61 0.14692707836275076 2304 62-63 0.16038771801393636 2304 64-65 -0.03523594539333885 2304 66-67 -0.13018522371324082 2304 68-69 -0.13831542561273835 2304 70-71 0.045194518932774486 2304 72-73 -0.11114143951309075 2304 74-75 -0.06442121925914535 2304 76-77 0.028598088463674287 2304 78-79 0.05719131135518296 2304 80-81 0.22964908045435806 2304 82-83 0.013843131363341143 2304 84-85 0.005848989773443947 2304 86-87 0.15286847212351162 2304 88-89 0.09879042381427183 2304 90-91 -0.08523354784740533 2304 92-93 -0.030256542008448406 2304 94-95 0.03550503589525533 2304 96-97 -0.05346458643128216 2304 98-99 -0.009866688586416927 2304 100-101 -0.03572189464997422 2202 1 0.0055270180413664605 2202 2 -0.05968239770958306 2202 3 0.06937567840120806 2202 4 0.18744194911391077 2202 5 0.17102020684806263 2202 6 -0.010028523730355232 2202 7 0.36938624796056985 2202 8 -0.04007866611968325 2202 9 0.10112626440445638 2202 10-11 0.2048980406587546 2202 12-13 0.13836906272803873 2202 14-15 0.21993565056128972 2202 16-17 0.26202666032774147 2202 18-19 0.19068743090802087 2202 20-21 0.22736054607756273 2202 22-23 0.08866993986126204 2202 24-25 0.14555554002706828 2202 26-27 0.12242765035781389 2202 28-29 0.18763958536563763 2202 30-31 0.1767186624162278 2202 32-33 0.13605440426827187 2202 34-35 -0.1679198462532554 2202 36-37 0.1477317742307278 2202 38-39 0.05306950083707562 2202 40-41 -0.07892374216633158 2202 42-43 0.17157012206808986 2202 44-45 0.2502938416168803 2202 46-47 0.21092461453302747 2202 48-49 0.17802721037549674 2202 50-51 0.1299549464642027 2202 52-53 0.22581387739988656 2202 54-55 0.30126582711027794 2202 56-57 0.2602951359479633 2202 58-59 0.22380298352293693 2202 60-61 0.4327459610310882 2202 62-63 0.4727248092218872 2202 64-65 0.34452805479605075 2202 66-67 0.35573759054255305 2202 68-69 0.2766108869071857 2202 70-71 0.3167747432763335 2202 72-73 0.22298203503755332 2202 74-75 0.2920860254927291 2202 76-77 0.2565051462651482 2202 78-79 0.39613235967465243 2202 80-81 0.5588001402897405 2202 82-83 0.4282467785770585 2202 84-85 0.5022345178204013 2202 86-87 0.5510033327273334 2202 88-89 0.4380404600183674 2202 90-91 0.40771949262576257 2202 92-93 0.4963670015574557 2202 94-95 0.47956465706238305 2202 96-97 0.4536854718731185 2202 98-99 0.44393025355164895 2202 100-101 0.35756778608002193 1102 1 -0.12864697705107986 1102 2 -0.19223088502656438 1102 3 -0.17121071712355018 1102 4 -0.06624159811413932 1102 5 -0.10219676271294986 1102 6 -0.11712016436446504 1102 7 0.16287245968338482 1102 8 -0.13561012816428786 1102 9 -0.0469775636948242 1102 10-11 -0.012186530012904484 1102 12-13 0.02954608550664517 1102 14-15 -0.0229848147097087 1102 16-17 0.053726379223071774 1102 18-19 0.10092047207222521 1102 20-21 0.09360680595070647 1102 22-23 -0.054542063503049576 1102 24-25 -0.14591259382880395 1102 26-27 -0.20165596963376942 1102 28-29 -0.22451830182725985 1102 30-31 -0.28930573414960037 1102 32-33 -0.3281101238782753 1102 34-35 -0.2731914021303652 1102 36-37 -0.15790062444512643 1102 38-39 -0.19300689774369317 1102 40-41 -0.38486259602962036 1102 42-43 -0.2405249937649927 1102 44-45 -0.09827302845651076 1102 46-47 -0.1217803884012909 1102 48-49 -0.1421457966018025 1102 50-51 -0.1255830603131045 1102 52-53 -0.12406378961782849 1102 54-55 -0.09395966369842057 1102 56-57 0.004609168108252959 1102 58-59 0.09936865524694838 1102 60-61 0.07533046430538093 1102 62-63 0.09878685152295219 1102 64-65 0.04957504293084014 1102 66-67 0.0849284221662785 1102 68-69 -0.03617751795584212 1102 70-71 -0.0047709068036567714 1102 72-73 -0.04577838148529878 1102 74-75 0.1390323696446032 1102 76-77 0.1887710058904517 1102 78-79 0.10469176835421479 1102 80-81 0.18530158210944592 1102 82-83 0.2251385856620267 1102 84-85 0.2183716110395686 1102 86-87 0.17967657819309935 1102 88-89 0.19029955286261568 1102 90-91 0.24192666686553466 1102 92-93 0.17404738488122717 1102 94-95 0.15200498405989293 1102 96-97 0.278692869926239 1102 98-99 0.1497709933598088 1102 100-101 0.035602379219842106 2305 1 -0.03922519369038824 2305 2 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-1.0097134702204293 1213 86-87 -0.9751019763428594 1213 88-89 -0.9186160084942365 1213 90-91 -0.8785086436058229 1213 92-93 -0.8768496295239103 1213 94-95 -1.018480783944831 1213 96-97 -0.8038543962840379 1213 98-99 -0.8698273067710218 1213 100-101 -0.6825768749283121 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 295.0 3 187.0 4 277.0 5 183.0 6 581.0 7 2999.0 8 14412.0 9 45009.0 10 62813.0 11 45011.0 12 23753.0 13 14945.0 14 10952.0 15 9111.0 16 8412.0 17 8290.0 18 8138.0 19 8306.0 20 9173.0 21 9773.0 22 10978.0 23 11691.0 24 13266.0 25 15465.0 26 18688.0 27 23116.0 28 28199.0 29 33605.0 30 40158.0 31 51354.0 32 69441.0 33 97717.0 34 149211.0 35 250693.0 36 492068.0 37 893077.0 38 309860.0 39 1129.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.15125450137647 23.90130894663934 19.977411046127607 14.970025505856588 2 32.973700552435844 25.73822239713174 27.87876330999439 13.409313740438025 3 37.00850375695272 20.563304411258915 25.95605848300952 16.472133348778843 4 30.693008235625314 18.694206014355412 30.254850734894628 20.357935015124646 5 35.2568829186559 16.749606936283513 28.79087719197576 19.202632953084834 6 33.297344822405485 17.128439541273778 31.928502506227467 17.64571313009327 7 36.708173304072204 18.71688138130495 25.092612043186215 19.482333271436634 8 29.866973861442638 29.875970348153285 21.632285272500358 18.624770517903723 9 32.291734244203866 26.12461267990559 22.351680373332663 19.231972702557883 10-11 27.234238807237748 30.008874548500852 26.46357036363073 16.293316280630677 12-13 29.02337385934896 25.364347942495723 26.37271043184839 19.239567766306926 14-15 26.50883459354059 25.56098190427204 27.884984506529836 20.045198995657525 16-17 27.74698339827422 26.034626338862584 28.32567202304289 17.892718239820308 18-19 27.251217021782924 26.158666022988207 28.728881988579367 17.8612349666495 20-21 25.623976465355764 28.077484988457208 28.094185884195056 18.20435266199197 22-23 28.187891676233136 26.16403062344685 27.258488166706545 18.38958953361347 24-25 25.149645241721064 28.92181504871508 27.45447243273945 18.474067276824407 26-27 23.180103595981418 30.844349623509338 27.56925072468348 18.406296055825766 28-29 24.365331432899907 29.808567189734074 27.200294029322254 18.625807348043764 30-31 26.35257630376666 27.658994785202573 27.43040879309432 18.558020117936444 32-33 23.09171402959425 29.731075759798188 28.891676075816353 18.28553413479121 34-35 26.82359238684498 27.82518442939662 27.53047076818521 17.82075241557319 36-37 23.946534642801367 29.14608730305473 29.2888339188362 17.618544135307708 38-39 23.016783267507094 31.36157440040907 27.589549481355775 18.032092850728063 40-41 27.25309351277982 27.006420430470474 27.017947042221152 18.722539014528557 42-43 27.13004133983521 26.90354235999653 27.35386442782518 18.61255187234308 44-45 24.28580944588591 28.47247994768704 28.554874292515546 18.6868363139115 46-47 24.58832455632326 28.967257936347597 27.721524101983917 18.72289340534522 48-49 21.631942474358155 29.527731114164297 28.971029616771034 19.869296794706514 50-51 20.325626064451434 28.04080238537685 30.409999217037832 21.22357233313388 52-53 25.028252209580486 27.421317169819083 27.317574589335074 20.23285603126536 54-55 26.476502867627723 27.157372572570416 27.419727519261894 18.94639704053997 56-57 26.96881672488433 27.019453791252822 26.81114151928796 19.200587964574883 58-59 25.58132895863965 27.424492455394983 26.919178707158796 20.074999878806572 60-61 22.25398216967351 27.437507157655826 26.88690167831098 23.421608994359676 62-63 24.408101799292993 27.493616902889872 26.827944010029874 21.27033728778726 64-65 22.400753412351648 28.638015482860744 28.766025223949736 20.195205880837864 66-67 21.049440201388204 28.546013485714777 28.010860660298853 22.393685652598165 68-69 20.846217724398432 27.52624717853826 32.02450179456994 19.60303330249337 70-71 20.263342340555564 30.806387200470976 29.824668780417323 19.105601678556138 72-73 20.01573966557249 34.12336164798068 26.63163977881911 19.229258907627724 74-75 20.31255552887178 33.995820818583965 26.373391659514102 19.31823199303015 76-77 20.600738812497386 33.463510700145186 26.266667121372056 19.66908336598537 78-79 20.276438057664105 33.26978064089977 26.852508332989306 19.60127296844682 80-81 19.716380549090847 33.270087392487135 26.965388554160096 20.04814350426192 82-83 19.648282503021413 32.77831972597471 26.962266939083168 20.611130831920708 84-85 19.702489232906046 32.29244841956943 27.474977323929195 20.530085023595323 86-87 20.007677140685217 31.87079853394621 28.0196962825475 20.101828042821065 88-89 20.456696639848555 32.09682886560328 27.650421877143227 19.796052617404936 90-91 20.894473388423936 31.4964313662059 27.05047215259251 20.558623092777655 92-93 20.68619925131653 31.80424029016914 26.860585388844626 20.648975069669703 94-95 20.30668463599282 31.838390212568225 27.214321904719856 20.6406032467191 96-97 20.623171655318217 32.136960290596875 26.862731022006543 20.377137032078362 98-99 20.959030948665898 31.61022316270794 26.726209752409236 20.704536136216927 100-101 21.122467591781398 31.53575316613862 26.417868540258578 20.92391070182141 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 96.0 1 113.0 2 133.5 3 133.0 4 114.0 5 113.0 6 164.0 7 265.0 8 398.5 9 548.5 10 694.5 11 809.0 12 925.5 13 922.0 14 803.5 15 717.0 16 634.5 17 578.5 18 527.0 19 529.5 20 631.0 21 788.0 22 1045.5 23 1404.0 24 1913.0 25 2667.0 26 3585.5 27 4768.5 28 6391.5 29 8363.5 30 10867.5 31 14433.0 32 20133.5 33 29381.5 34 42683.0 35 61466.5 36 87908.0 37 120508.5 38 152250.0 39 172242.5 40 178995.5 41 172074.0 42 161463.0 43 158390.5 44 150872.0 45 143359.5 46 144967.5 47 138640.5 48 119200.5 49 99703.0 50 84892.0 51 75246.5 52 67971.5 53 59715.5 54 51244.5 55 43328.0 56 36159.5 57 29739.0 58 24103.5 59 19590.0 60 16121.5 61 13241.0 62 10718.0 63 8539.0 64 6705.0 65 5295.0 66 4254.5 67 3380.5 68 2640.0 69 2069.0 70 1595.0 71 1233.0 72 961.5 73 766.0 74 582.0 75 414.5 76 331.5 77 262.5 78 183.5 79 159.0 80 136.5 81 90.5 82 70.0 83 61.0 84 41.5 85 33.5 86 41.5 87 32.0 88 24.5 89 21.5 90 17.5 91 14.0 92 10.5 93 9.5 94 7.0 95 4.5 96 3.5 97 2.5 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18382458271497412 2 0.1741552592524682 3 0.14464591653726486 4 0.13755507933142716 5 0.14267623953564326 6 0.1247342726663267 7 0.09819735160811593 8 0.08444542490588526 9 0.08347849255963466 10-11 0.06344150560677511 12-13 0.08299502638650937 14-15 0.08777596965408174 16-17 0.08002260472951679 18-19 0.07602953226259304 20-21 0.07400613679729087 22-23 0.09466983915975728 24-25 0.44208504993668385 26-27 0.2747520355716504 28-29 0.12528936345769276 30-31 0.23326347545567583 32-33 0.2639188120627317 34-35 0.2478390852676755 36-37 0.25000572996945924 38-39 0.26784025991141464 40-41 0.26773282298405354 42-43 0.29428765019682446 44-45 0.2706515261773655 46-47 0.28913067768348794 48-49 0.27324791858859393 50-51 0.28785934070971403 52-53 0.287948871482515 54-55 0.26388299975361135 56-57 0.2798552896213063 58-59 0.2697204061402353 60-61 0.2455650036385306 62-63 0.23845626027813271 64-65 0.2367372694403539 66-67 0.24603056365709572 68-69 0.2502922284424224 70-71 0.2366656448221131 72-73 0.22851834449722383 74-75 0.2371849233043588 76-77 0.23813394949604919 78-79 0.24069452959815724 80-81 0.24758839910383276 82-83 0.2732300124340337 84-85 0.267231450656368 86-87 0.2666226414013213 88-89 0.26508271210914447 90-91 0.256648913311292 92-93 0.2332276631465554 94-95 0.23197423232734168 96-97 0.2272649136780101 98-99 0.2511875361704322 100-101 0.2592811180316409 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2792336.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.188402936249076 #Duplication Level Percentage of deduplicated Percentage of total 1 91.79744725096653 55.25141743660244 2 4.034656612039297 4.856790757496455 3 1.1958817873519738 2.1593464464378704 4 0.6036987078244689 1.4534264431452815 5 0.39083631227101673 1.1761906722542814 6 0.339120894797128 1.2246687036090524 7 0.21903734469552352 0.9228455572434174 8 0.16279692409570515 0.7838789491403406 9 0.1406060932952251 0.7616570578690355 >10 0.9093812949882705 10.773526231797184 >50 0.09786494859309884 4.203869751968484 >100 0.10001806659551732 11.665190784957586 >500 0.006468163590138484 2.5316643896557887 >1k 0.0021259229657338377 1.8927255264916663 >5k 5.9675930411657915E-5 0.342801291331074 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 9544 0.34179267824502496 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2796 0.10013121630061711 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004763037113012188 0.0 0.0 2 0.005300221749818074 0.0 0.0 3 0.005980655623105529 0.0 0.0 4 0.006482027950791022 0.0 0.0 5 0.008057769552088288 0.0 0.0 6 0.01006325886283026 0.0 0.0 7 0.011889686627970273 0.0 0.0 8 0.013895175938712246 0.0 0.0 9 0.01604391448593579 0.0 0.0 10-11 0.02578486256668252 0.0 0.0 12-13 0.03402169366437277 0.0 0.0 14-15 0.04227643091662321 0.0 0.0 16-17 0.04961795428630365 0.0 0.0 18-19 0.05701319611966468 0.0 0.0 20-21 0.06374591023429844 0.0 0.0 22-23 0.0705681551217332 0.0 0.0 24-25 0.0776231800184505 0.0 1.7906154560196196E-5 26-27 0.08543026340669604 0.0 3.581230912039239E-5 28-29 0.09454449607783591 0.0 3.581230912039239E-5 30-31 0.19508755393333754 0.0 3.581230912039239E-5 32-33 0.2093229468086935 0.0 3.581230912039239E-5 34-35 0.22037104417233455 0.0 3.581230912039239E-5 36-37 0.23118636152669308 0.0 7.162461824078478E-5 38-39 0.24398926203723334 0.0 7.162461824078478E-5 40-41 0.2591378687951593 0.0 7.162461824078478E-5 42-43 0.2790853249752179 0.0 7.162461824078478E-5 44-45 0.30032202428361054 0.0 7.162461824078478E-5 46-47 0.3218273159104062 0.0 7.162461824078478E-5 48-49 0.3467884953673197 0.0 8.953077280098097E-5 50-51 0.37608296422780063 0.0 1.2534308192137337E-4 52-53 0.4087796024547189 0.0 1.4324923648156957E-4 54-55 0.44380404077446267 0.0 1.4324923648156957E-4 56-57 0.4832692054251351 0.0 1.4324923648156957E-4 58-59 0.5271750964067361 0.0 1.4324923648156957E-4 60-61 0.5707407704516935 0.0 1.4324923648156957E-4 62-63 0.6200364139559136 0.0 1.4324923648156957E-4 64-65 0.6718031067894408 0.0 1.4324923648156957E-4 66-67 0.7263094412706781 0.0 1.4324923648156957E-4 68-69 0.7846118805186768 0.0 1.6115539104176577E-4 70-71 0.8471580783974422 0.0 1.7906154560196194E-4 72-73 0.9180664504558191 0.0 1.7906154560196194E-4 74-75 0.9905147518063728 0.0 1.7906154560196194E-4 76-77 1.0703403888357275 0.0 1.7906154560196194E-4 78-79 1.1542844414139273 0.0 1.7906154560196194E-4 80-81 1.2473069143541464 0.0 1.7906154560196194E-4 82-83 1.3469725706361986 0.0 1.7906154560196194E-4 84-85 1.4570417027177245 0.0 1.7906154560196194E-4 86-87 1.5744702643234911 0.0 2.1487385472235434E-4 88-89 1.6985061969619704 0.0 2.1487385472235434E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2070 0.0 84.96772 9 CGGGAGA 3250 0.0 79.26159 4 ATCGGGA 3920 0.0 78.18788 2 GAGAGCG 2850 0.0 77.52713 7 TCGGAGA 925 0.0 77.44288 4 AGAGCGG 2375 0.0 77.055786 8 TCGGGAG 3470 0.0 76.42379 3 GTTCGGA 655 0.0 75.33296 2 CGGAAAG 1625 0.0 74.15131 5 TTCGGAG 660 0.0 73.316635 3 ATTCGGA 590 0.0 72.37416 2 AAGCGTC 330 0.0 71.83009 9 AAGCGGC 795 0.0 70.36639 9 GGATCGG 1535 0.0 69.86181 1 GATCGGG 4570 0.0 69.047066 1 TCGGAAA 1290 0.0 68.40211 4 TCGGGAA 1020 0.0 65.11397 3 ATCGGAG 770 0.0 64.69115 3 AAAGCGG 1020 0.0 62.750195 8 ATCCGGA 730 0.0 61.743862 2 >>END_MODULE