FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_34139-1.342000000356e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_34139-1.342000000356e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792336
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT95440.34179267824502496Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC27960.10013121630061711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC20700.084.967729
CGGGAGA32500.079.261594
ATCGGGA39200.078.187882
GAGAGCG28500.077.527137
TCGGAGA9250.077.442884
AGAGCGG23750.077.0557868
TCGGGAG34700.076.423793
GTTCGGA6550.075.332962
CGGAAAG16250.074.151315
TTCGGAG6600.073.3166353
ATTCGGA5900.072.374162
AAGCGTC3300.071.830099
AAGCGGC7950.070.366399
GGATCGG15350.069.861811
GATCGGG45700.069.0470661
TCGGAAA12900.068.402114
TCGGGAA10200.065.113973
ATCGGAG7700.064.691153
AAAGCGG10200.062.7501958
ATCCGGA7300.061.7438622
Produced by FastQC (version 0.11.2)