##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_34098-1.34200000035704.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2352785 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.149356613545223 33.0 33.0 33.0 27.0 33.0 2 30.753997921612047 33.0 33.0 33.0 27.0 33.0 3 30.606407725312767 33.0 33.0 33.0 27.0 33.0 4 34.09162800680895 37.0 37.0 37.0 27.0 37.0 5 34.17550817435507 37.0 37.0 37.0 27.0 37.0 6 34.58873207709162 37.0 37.0 37.0 27.0 37.0 7 34.10653459623383 37.0 37.0 37.0 27.0 37.0 8 34.29690813227728 37.0 37.0 37.0 27.0 37.0 9 33.85785228994575 37.0 37.0 37.0 27.0 37.0 10-11 33.85006237289 37.0 37.0 37.0 24.5 37.0 12-13 33.840551516606915 37.0 37.0 37.0 24.5 37.0 14-15 35.39304080058314 40.0 37.0 40.0 24.5 40.0 16-17 35.295977958036964 40.0 37.0 40.0 24.5 40.0 18-19 35.2973661001749 40.0 37.0 40.0 22.0 40.0 20-21 35.24420952190702 40.0 37.0 40.0 22.0 40.0 22-23 35.85955431541768 40.0 37.0 40.0 27.0 40.0 24-25 35.319214250345865 40.0 37.0 40.0 22.0 40.0 26-27 35.18610774040127 37.0 37.0 40.0 24.5 40.0 28-29 34.99305950182443 37.0 37.0 40.0 22.0 40.0 30-31 34.97841706743285 37.0 37.0 40.0 18.5 40.0 32-33 34.78436937501726 37.0 37.0 40.0 15.0 40.0 34-35 34.847618248161226 37.0 37.0 40.0 18.5 40.0 36-37 34.70155730336601 37.0 37.0 40.0 18.5 40.0 38-39 34.81731352418517 37.0 37.0 40.0 18.5 40.0 40-41 34.516548473404924 37.0 37.0 40.0 15.0 40.0 42-43 34.3828248224976 37.0 37.0 40.0 15.0 40.0 44-45 34.2307259269334 37.0 33.0 40.0 15.0 40.0 46-47 34.16972927828085 37.0 33.0 40.0 15.0 40.0 48-49 34.06697679558481 37.0 33.0 40.0 15.0 40.0 50-51 33.005837762481484 37.0 33.0 38.5 15.0 38.5 52-53 33.45056921903192 37.0 33.0 38.5 18.5 40.0 54-55 34.15535503669056 37.0 33.0 40.0 18.5 40.0 56-57 34.34182787632529 37.0 33.0 40.0 18.5 40.0 58-59 33.877851992426 37.0 33.0 40.0 15.0 40.0 60-61 33.66318809410975 37.0 33.0 40.0 15.0 40.0 62-63 33.32184857519918 37.0 33.0 40.0 15.0 40.0 64-65 33.09692555843394 37.0 33.0 37.0 15.0 40.0 66-67 32.952003901758985 37.0 33.0 37.0 15.0 40.0 68-69 32.969749254606775 37.0 33.0 37.0 18.5 40.0 70-71 32.542785252371125 37.0 33.0 37.0 15.0 38.5 72-73 32.67284324747055 37.0 33.0 37.0 22.0 37.0 74-75 32.671320371389655 37.0 33.0 37.0 22.0 37.0 76-77 32.38038898581893 37.0 33.0 37.0 22.0 37.0 78-79 31.965644544656648 37.0 33.0 37.0 22.0 37.0 80-81 31.56589042347686 35.0 33.0 37.0 15.0 37.0 82-83 31.274725697418166 33.0 33.0 37.0 15.0 37.0 84-85 31.084189163055697 33.0 33.0 37.0 15.0 37.0 86-87 30.942712997575214 33.0 33.0 37.0 15.0 37.0 88-89 30.80439712935946 33.0 33.0 37.0 15.0 37.0 90-91 30.654565759302272 33.0 30.0 37.0 15.0 37.0 92-93 30.506067915257873 33.0 27.0 37.0 15.0 37.0 94-95 30.38743085322288 33.0 27.0 37.0 15.0 37.0 96-97 30.239110033428467 33.0 27.0 37.0 15.0 37.0 98-99 30.081602016333832 33.0 27.0 37.0 15.0 37.0 100-101 28.859798069096836 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.007412907260935242 2306 2 -0.04400988095823166 2306 3 -0.07459871983899191 2306 4 0.028246350283609445 2306 5 -0.04260790018131644 2306 6 -0.18959582770216343 2306 7 0.30345651881687274 2306 8 -0.13949386542086728 2306 9 0.01436108691275706 2306 10-11 -0.009841987747584824 2306 12-13 0.040337344904031625 2306 14-15 0.2884345483397155 2306 16-17 0.2288729966448244 2306 18-19 0.2578908935798552 2306 20-21 0.3135604606413267 2306 22-23 0.12651557079810516 2306 24-25 0.21076793863529986 2306 26-27 0.06882583941214904 2306 28-29 0.1971779629691568 2306 30-31 0.212109708058712 2306 32-33 0.09997298799265764 2306 34-35 0.15863145850987337 2306 36-37 0.1118926427871898 2306 38-39 -0.16067296461520897 2306 40-41 0.027586025144486825 2306 42-43 0.058991443386069875 2306 44-45 0.06375816787207356 2306 46-47 0.1089370950871924 2306 48-49 0.10553947967034816 2306 50-51 0.0227847769613021 2306 52-53 0.0747370195438748 2306 54-55 0.198919235944075 2306 56-57 0.1411468586878044 2306 58-59 0.31131209553206673 2306 60-61 0.20493734148028864 2306 62-63 0.20483059922880642 2306 64-65 0.18120175927565896 2306 66-67 0.16864324688844334 2306 68-69 3.380748069403694E-4 2306 70-71 0.17462541231205364 2306 72-73 -0.075266795798413 2306 74-75 -0.008661436095970032 2306 76-77 0.1147805011902463 2306 78-79 0.4172972366485439 2306 80-81 0.3335616927201208 2306 82-83 0.011007023874160637 2306 84-85 0.12267923915950263 2306 86-87 0.09070150079163497 2306 88-89 0.22746317037144692 2306 90-91 0.0919193131590994 2306 92-93 0.12284502603584713 2306 94-95 -0.04609318491726455 2306 96-97 0.017833156459541755 2306 98-99 -0.12652301507675645 2306 100-101 -0.21988843872035702 2307 1 0.13448639682991725 2307 2 0.08285214144377662 2307 3 0.15357222683782368 2307 4 0.30246884325499934 2307 5 0.17821864805745946 2307 6 -0.030164762298959147 2307 7 0.33768049645728837 2307 8 -0.11984350497622387 2307 9 0.19417807199772597 2307 10-11 0.1788254216463585 2307 12-13 0.06071722575345717 2307 14-15 0.4050034546570984 2307 16-17 0.4312311754036884 2307 18-19 0.5798915970301763 2307 20-21 0.5836381052964015 2307 22-23 0.26582421581373694 2307 24-25 0.39431589108818343 2307 26-27 0.11635925624829468 2307 28-29 0.22096772205215132 2307 30-31 0.3321029268918565 2307 32-33 0.2797790737216914 2307 34-35 0.2058443753660555 2307 36-37 0.16407728612491468 2307 38-39 0.18437781460036717 2307 40-41 0.23782267078227193 2307 42-43 0.11205805082308729 2307 44-45 0.23101217235435456 2307 46-47 0.21514940393605997 2307 48-49 0.11436398118682689 2307 50-51 -0.03527344091741469 2307 52-53 -0.04344749535820114 2307 54-55 0.10308856362466656 2307 56-57 0.14756245525374112 2307 58-59 0.36402417932395537 2307 60-61 0.34188259372084673 2307 62-63 0.22187321512135583 2307 64-65 0.30915298082833687 2307 66-67 0.19106926088203835 2307 68-69 0.13410395546311804 2307 70-71 -0.044597457718126066 2307 72-73 -0.235038097853149 2307 74-75 -0.20053443444398766 2307 76-77 0.06929778344468218 2307 78-79 0.23564503568562856 2307 80-81 0.26922868617108264 2307 82-83 0.18511274644843567 2307 84-85 0.1387504867763738 2307 86-87 0.18484203649792263 2307 88-89 0.22520229045760232 2307 90-91 0.3813665861991602 2307 92-93 0.14802268553761166 2307 94-95 0.09030857241240398 2307 96-97 0.09474814383985475 2307 98-99 0.17307244207168893 2307 100-101 0.21117946839120805 2201 1 0.062188650004376456 2201 2 -0.021904338110328325 2201 3 0.08998793481316625 2201 4 0.15421733062139964 2201 5 -0.04777009606404192 2201 6 -0.04286631590714762 2201 7 0.343420937383911 2201 8 0.06055407709432359 2201 9 0.11281537401332997 2201 10-11 0.08357804644755618 2201 12-13 0.1047567783736838 2201 14-15 0.3776919592128465 2201 16-17 0.33695419855789766 2201 18-19 0.3819952927388428 2201 20-21 0.3513111480099198 2201 22-23 0.3884217767545266 2201 24-25 0.3757372669779926 2201 26-27 0.2154649535730968 2201 28-29 0.2362880805727272 2201 30-31 0.3342097635102945 2201 32-33 0.4270951663157092 2201 34-35 0.22275746068172708 2201 36-37 0.33111643613762 2201 38-39 0.2605537283909527 2201 40-41 0.3879909949926841 2201 42-43 0.32085288374096166 2201 44-45 0.43277950517943253 2201 46-47 0.36688775472999424 2201 48-49 0.27493742565125956 2201 50-51 0.2752350438220432 2201 52-53 0.1913340829204131 2201 54-55 0.382156684247029 2201 56-57 0.41571248794775784 2201 58-59 0.5297217325552381 2201 60-61 0.4151805775423014 2201 62-63 0.4999084047323734 2201 64-65 0.3861886472865592 2201 66-67 0.2789534869475929 2201 68-69 0.22342586159560085 2201 70-71 0.42949417689698066 2201 72-73 0.3551247154882944 2201 74-75 0.3737936250162974 2201 76-77 0.43454090413841584 2201 78-79 0.5635207527410557 2201 80-81 0.47281274976593934 2201 82-83 0.45127758139362584 2201 84-85 0.3682887444851666 2201 86-87 0.3445730032985068 2201 88-89 0.5814065866511058 2201 90-91 0.5858990617785835 2201 92-93 0.4106113091623911 2201 94-95 0.35402378326739026 2201 96-97 0.4993741328695158 2201 98-99 0.49651105299006204 2201 100-101 0.26010884646251853 1101 1 -0.08383373141623451 1101 2 -0.04871053748290066 1101 3 -0.041032339569941456 1101 4 0.0068277159751488625 1101 5 -0.006725005461426292 1101 6 -0.1441071268824743 1101 7 0.12490241886539621 1101 8 -0.1618694842672923 1101 9 0.09556576030353625 1101 10-11 0.0043300247216606635 1101 12-13 -0.04335157017714408 1101 14-15 0.09887002729357164 1101 16-17 0.2385976878120104 1101 18-19 0.20506969457430557 1101 20-21 0.22571298347369861 1101 22-23 0.08867228010237227 1101 24-25 0.14162432976770845 1101 26-27 0.044022335227822396 1101 28-29 0.14786508108777952 1101 30-31 0.1314495443783983 1101 32-33 0.11907671803177067 1101 34-35 0.13441406039142834 1101 36-37 0.14914097141879523 1101 38-39 0.050910520075184706 1101 40-41 0.11383029826561852 1101 42-43 0.20092733239948046 1101 44-45 0.28741264653823606 1101 46-47 0.15523115502030294 1101 48-49 0.24702854331759738 1101 50-51 0.22425175965291544 1101 52-53 0.24760369007497474 1101 54-55 0.41500774010692965 1101 56-57 0.24552472749450516 1101 58-59 0.37438156305578474 1101 60-61 0.4128651774392935 1101 62-63 0.4199393079491145 1101 64-65 0.3208611200121325 1101 66-67 0.12482135228447788 1101 68-69 0.22267435155205106 1101 70-71 0.3088335035814396 1101 72-73 0.1245422366757225 1101 74-75 0.3156417310236961 1101 76-77 0.39157606825322944 1101 78-79 0.4101448104270631 1101 80-81 0.2819924098305542 1101 82-83 0.4768336051797384 1101 84-85 0.3532491790031216 1101 86-87 0.385788998054327 1101 88-89 0.4754670820390352 1101 90-91 0.4648263455731261 1101 92-93 0.4512654272501422 1101 94-95 0.413674015041515 1101 96-97 0.414441792184963 1101 98-99 0.39370166964970466 1101 100-101 0.20199441091445536 2304 1 0.0872383616520267 2304 2 -0.008691701186354095 2304 3 0.07579693299329548 2304 4 0.19075254035249145 2304 5 0.17229952503650736 2304 6 0.05440338651719401 2304 7 0.28105153893204005 2304 8 -0.05167622791948645 2304 9 0.07230422422382787 2304 10-11 0.1052429031639619 2304 12-13 0.07035483056967706 2304 14-15 0.17438892395104943 2304 16-17 0.09391184572173472 2304 18-19 0.16860017315815412 2304 20-21 0.2625177154020548 2304 22-23 0.11939622422382001 2304 24-25 -0.015153972366860557 2304 26-27 0.11116718948151316 2304 28-29 0.0532138428593143 2304 30-31 0.029308021495154435 2304 32-33 -0.18237037768592046 2304 34-35 -0.14787696407640993 2304 36-37 0.013950917425709974 2304 38-39 0.024068106338326345 2304 40-41 0.18350132658549256 2304 42-43 0.043190397315591156 2304 44-45 0.2088879137620694 2304 46-47 0.0277804321175239 2304 48-49 0.06601486672870038 2304 50-51 0.11990337663470996 2304 52-53 0.04310039602692939 2304 54-55 0.32342454200972526 2304 56-57 0.3072714253829858 2304 58-59 0.3056553445713277 2304 60-61 0.17790080881923842 2304 62-63 0.16002652258244865 2304 64-65 0.16347897818513957 2304 66-67 0.0620187988788814 2304 68-69 0.11824072599809199 2304 70-71 0.27900276300350413 2304 72-73 0.12259594223786507 2304 74-75 0.14311521598737187 2304 76-77 0.11163635546067496 2304 78-79 0.20366619340195768 2304 80-81 0.1385637799499584 2304 82-83 0.09410844278118802 2304 84-85 0.06819998254073312 2304 86-87 0.12299294151226192 2304 88-89 0.10000965216138624 2304 90-91 0.07847330693014953 2304 92-93 0.2749976864353698 2304 94-95 0.22377207621155648 2304 96-97 0.18154513101989522 2304 98-99 0.12041801658774531 2304 100-101 0.08650436770340164 2202 1 -0.06274682109906138 2202 2 -0.10395134282828522 2202 3 -0.02551842580563246 2202 4 -0.08113989115160791 2202 5 -0.10842045992688298 2202 6 -0.06140814495838498 2202 7 0.30237934508027564 2202 8 -0.039578987196613014 2202 9 -0.14471793455255977 2202 10-11 0.04671401727053848 2202 12-13 -0.03795151701933008 2202 14-15 0.10780904607614872 2202 16-17 0.17481778823425032 2202 18-19 0.22345076840725397 2202 20-21 0.16472623818040688 2202 22-23 -0.014818265884322557 2202 24-25 -0.18604620731122168 2202 26-27 -0.07749380863761957 2202 28-29 0.012801140541498057 2202 30-31 0.03673567826659507 2202 32-33 0.09148711349792649 2202 34-35 -0.212530922174345 2202 36-37 -0.15160183907720892 2202 38-39 -0.24102774556316575 2202 40-41 -0.14584934242727599 2202 42-43 -0.22049781593924678 2202 44-45 -0.041776926949282256 2202 46-47 -0.06844749200956102 2202 48-49 -0.010250309123435386 2202 50-51 -0.010550994625759813 2202 52-53 -0.04606062510302422 2202 54-55 0.10521614310271588 2202 56-57 0.09439476295862193 2202 58-59 0.03367043967053718 2202 60-61 0.25935427986194526 2202 62-63 0.15080294642544345 2202 64-65 0.013868205163454661 2202 66-67 0.03490398606009393 2202 68-69 0.10053164042234641 2202 70-71 0.256536458803879 2202 72-73 0.14582611970507742 2202 74-75 0.013932784040257218 2202 76-77 0.1029035334693873 2202 78-79 0.2335469285267564 2202 80-81 0.24295559962425628 2202 82-83 0.17990085161855163 2202 84-85 0.08710520074999195 2202 86-87 0.13659448783949912 2202 88-89 0.13376082904665054 2202 90-91 0.3048826982702515 2202 92-93 0.05559068176911808 2202 94-95 -0.009049054862817485 2202 96-97 0.28537931460668275 2202 98-99 0.24232644814001603 2202 100-101 -0.007934841360885514 1102 1 0.032798798264700224 1102 2 -0.010892316497599808 1102 3 0.04183931926172235 1102 4 0.2181968957493794 1102 5 0.12224504780269285 1102 6 0.11323797111586487 1102 7 0.24376775062825118 1102 8 0.06730478287329333 1102 9 0.16112571110458873 1102 10-11 0.1428903559807111 1102 12-13 0.19098754472812374 1102 14-15 0.2547277342609888 1102 16-17 0.10343998014968747 1102 18-19 0.13488431250065247 1102 20-21 0.20927967307208206 1102 22-23 0.10667107097555117 1102 24-25 -0.017275521176145503 1102 26-27 -0.05034145733048234 1102 28-29 -0.017116017619358104 1102 30-31 0.138348385979306 1102 32-33 0.14139887562824782 1102 34-35 0.006304163618885639 1102 36-37 0.10362868210393117 1102 38-39 -0.024309415924484767 1102 40-41 0.07319838183435223 1102 42-43 0.019167427461866282 1102 44-45 0.07585562647994237 1102 46-47 0.068922127568257 1102 48-49 0.11343696759603006 1102 50-51 0.024312389719185035 1102 52-53 0.11083555056675465 1102 54-55 0.20449155066315683 1102 56-57 0.34040774099990045 1102 58-59 0.2794955093608209 1102 60-61 0.2898297913699608 1102 62-63 0.27815311840111434 1102 64-65 0.16261773285875591 1102 66-67 0.1733427778033132 1102 68-69 -0.03818303402389489 1102 70-71 0.03674196607939706 1102 72-73 0.13339700595139448 1102 74-75 0.23002130650899488 1102 76-77 0.21436754425658222 1102 78-79 0.12479218318210172 1102 80-81 0.03796573086414767 1102 82-83 0.20342740284556626 1102 84-85 0.2714565703120009 1102 86-87 0.28159040939718594 1102 88-89 0.22810835530517082 1102 90-91 0.31436345499562535 1102 92-93 0.13781254497670403 1102 94-95 0.24204119871413 1102 96-97 0.12705071783408428 1102 98-99 0.11036208221211652 1102 100-101 0.06975137642306706 2305 1 0.021928909744637792 2305 2 -0.1696917358977288 2305 3 -0.041397346572118465 2305 4 -0.06528723888316534 2305 5 0.01894467065072547 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-1.4359325962309732 1213 96-97 -1.273015705169179 1213 98-99 -1.2046982042497305 1213 100-101 -1.1505402104460813 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 255.0 3 174.0 4 216.0 5 210.0 6 505.0 7 2727.0 8 14448.0 9 43468.0 10 50425.0 11 32861.0 12 18608.0 13 12214.0 14 9278.0 15 7740.0 16 7176.0 17 6943.0 18 7511.0 19 7891.0 20 8390.0 21 9438.0 22 10313.0 23 11174.0 24 12530.0 25 14282.0 26 17085.0 27 20917.0 28 25705.0 29 31049.0 30 37695.0 31 49357.0 32 67567.0 33 96642.0 34 145121.0 35 245747.0 36 470755.0 37 703822.0 38 152459.0 39 87.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.013344381266094 23.595029111529904 22.332337259437082 14.059289247766923 2 32.568458536593674 24.83602223098322 30.007995592631794 12.587523639791314 3 36.58548212719951 20.05737683984711 27.632737147673005 15.724403885280372 4 30.156728417708646 17.575850953079367 32.697649158857345 19.569771470354645 5 34.08220299882696 15.939504190764483 32.155115320508784 17.823177489899773 6 31.135217347638772 16.503253788710552 36.20144211864796 16.160086745002715 7 36.14749749730585 18.253351355283865 27.93972431861413 17.65942682879615 8 29.657470380346467 29.681164401626 23.320659655164786 17.340705562862748 9 31.41939647159765 26.860795581436008 23.50050557063547 18.21930237633087 10-11 26.580667743088583 29.040383258938583 27.754886268645734 16.624062729327097 12-13 28.33501288697618 24.52706988148406 27.525785650387117 19.612131581152642 14-15 25.87791448807234 24.667090367901142 29.135330429533507 20.31966471449301 16-17 26.841496090222428 25.52357585453407 29.85582974357491 17.77909831166859 18-19 26.38591230280893 25.71132343323816 29.890397120358998 18.012367143593917 20-21 25.06294009685055 27.730711236918047 29.041319923693504 18.165028742537892 22-23 27.539264454222224 25.580482160159196 28.512220984932362 18.368032400686218 24-25 24.56086502998999 28.132257773267938 28.707340755076295 18.59953644166578 26-27 22.559783836308853 29.838111636272195 29.046974688561477 18.55512983885747 28-29 23.380014549713188 29.05712624227379 28.4001975424645 19.162661665548523 30-31 25.45173222774759 27.264667904656864 28.20178728976852 19.081812577827026 32-33 22.946503088765493 26.998897790232412 29.717667678961 20.336931442041088 34-35 20.592445296690265 27.002390070905363 33.47963348127387 18.925531151130496 36-37 21.399130488691604 27.715752846086865 30.47759209237689 20.407524572844636 38-39 20.481158821191787 27.369138800075433 28.22647129955072 23.923231079182067 40-41 22.008115751307876 26.60872481619809 29.35139191697762 22.03176751551641 42-43 23.985383226730494 26.74080910170145 30.459518273097935 18.81428939847012 44-45 22.025759889025146 28.415550543314396 30.869633312060312 18.68905625560015 46-47 23.30181447114103 29.099460775757436 29.09651966886546 18.502205084236074 48-49 20.84705997673041 28.95872844046863 30.42196376592326 19.772247816877698 50-51 19.86404866030707 27.733369990537327 30.945627989053985 21.456953360101615 52-53 24.127292512854524 27.0278564794351 28.377075446887357 20.46777556082301 54-55 25.913899176731135 26.937136418705876 28.225542173192856 18.923422231370125 56-57 26.339365533243303 26.89375623729499 27.35580887020864 19.411069359253062 58-59 24.665531920696772 26.847559777659114 27.79847064995459 20.688437651689526 60-61 21.260329979906707 27.165184239633376 27.57951251186595 23.994973268593963 62-63 23.397469147176515 27.263383580450594 27.78141918195151 21.557728090421378 64-65 21.62257328034089 28.365370073017264 29.659648959869262 20.35240768677258 66-67 20.844990729826694 28.26860686646839 28.693375303866333 22.193027099838584 68-69 20.93073544193284 27.022504728519696 32.485345743309665 19.561414086237804 70-71 20.412179684081774 30.8394110051196 29.88084369260578 18.86756561819284 72-73 20.163271026624034 33.91402104371708 27.054439792344574 18.868268137314313 74-75 20.868337314616827 33.39779220895412 26.607328714277617 19.126541762151437 76-77 20.903157008582912 32.71633445835119 26.858665264725705 19.52184326834019 78-79 20.81665545458767 32.41804708732342 27.398216832092764 19.367080625996145 80-81 19.884231574345776 32.53524799393953 27.574580967382794 20.0059394643319 82-83 19.272507207804246 32.11795441130405 27.983370375658183 20.626168005233517 84-85 19.40649704386358 31.508491474433104 28.276525696450484 20.808485785252834 86-87 19.403766459322405 31.53595199482822 29.006654576734103 20.053626969115275 88-89 19.769100368021192 31.691968478703693 28.87574980695507 19.663181346320044 90-91 20.50436052426428 30.96791430519984 27.988188990637653 20.53953617989823 92-93 19.93004892740979 31.645873760040555 27.75391877240554 20.670158540144122 94-95 19.762789946710598 31.747985299298666 27.947563465681398 20.541661288309346 96-97 20.243201012829918 32.24878827611884 27.386897961304925 20.121112749746324 98-99 20.707560495167503 31.421269466446066 27.223091682172786 20.64807835621365 100-101 20.699707274585556 31.469590668069745 26.858829818490644 20.971872238854054 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 76.0 1 89.0 2 108.0 3 120.5 4 119.0 5 129.5 6 186.0 7 261.5 8 384.0 9 525.5 10 651.0 11 755.0 12 778.0 13 733.5 14 679.0 15 625.0 16 525.5 17 460.5 18 472.0 19 484.5 20 533.5 21 602.5 22 688.5 23 872.5 24 1106.0 25 1368.0 26 1829.0 27 2591.5 28 3431.5 29 4291.5 30 5385.5 31 6962.5 32 11131.0 33 19634.5 34 34156.5 35 54457.5 36 82462.0 37 117552.0 38 147060.0 39 163104.0 40 166628.5 41 154327.5 42 143355.5 43 146088.5 44 142771.5 45 132919.5 46 128090.5 47 115800.0 48 94365.5 49 79231.5 50 68850.0 51 58700.5 52 49230.0 53 41936.5 54 34946.0 55 26961.0 56 21098.0 57 17099.5 58 13935.0 59 11271.0 60 8766.5 61 6812.0 62 5244.5 63 3964.5 64 3084.5 65 2355.0 66 1765.0 67 1379.5 68 1119.5 69 847.0 70 593.0 71 437.5 72 326.5 73 250.5 74 181.5 75 130.0 76 101.5 77 79.0 78 64.0 79 55.5 80 50.5 81 42.0 82 29.5 83 25.5 84 21.5 85 13.0 86 12.0 87 15.0 88 11.0 89 7.0 90 9.0 91 10.0 92 8.0 93 6.5 94 5.5 95 4.5 96 2.5 97 1.5 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17982943617882635 2 0.16962875910888586 3 0.14472210592978108 4 0.13783664890757125 5 0.14166190280879895 6 0.12364070665190402 7 0.09852153936717549 8 0.08419808864813402 9 0.08398557454251027 10-11 0.06275541539069657 12-13 0.08339053504676372 14-15 0.08806584537048646 16-17 0.08007531499903306 18-19 0.076398820971742 20-21 0.07395490875706875 22-23 0.0942500058441379 24-25 0.4358026764026462 26-27 0.27265559751528506 28-29 0.12370446088359115 30-31 0.2308753243496537 32-33 0.2607760590109168 34-35 0.24581506597500408 36-37 0.24798270985236645 38-39 0.2661739172937604 40-41 0.2650900953550792 42-43 0.29114432470455226 44-45 0.2680865442443742 46-47 0.2860227347590196 48-49 0.27095548467029495 50-51 0.28583147206395826 52-53 0.2857889692428335 54-55 0.2619873894129723 56-57 0.27692713103832267 58-59 0.2676827674436891 60-61 0.24366867350820412 62-63 0.23599691429518635 64-65 0.2351256064621289 66-67 0.2440299474877645 68-69 0.24889652050654865 70-71 0.2351256064621289 72-73 0.2269013105744894 74-75 0.23597566288462396 76-77 0.23644319391699625 78-79 0.23861083779435863 80-81 0.24532628353206945 82-83 0.2707429705646712 84-85 0.2646013129121445 86-87 0.26370875366852475 88-89 0.2627099373720931 90-91 0.2546556527689525 92-93 0.2307903187074042 94-95 0.2301102735694082 96-97 0.22539246042456068 98-99 0.24915153743329715 100-101 0.2569933079308139 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2352785.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.457999611739744 #Duplication Level Percentage of deduplicated Percentage of total 1 87.96961206263579 38.22983366862922 2 4.899665977616474 4.258593643058224 3 2.0237135035800073 2.638396219585573 4 0.9825693069333344 1.7080198623686493 5 0.6922955933012002 1.5042890812446348 6 0.490089592039705 1.2778987980347514 7 0.2949948908080582 0.897392149514126 8 0.283862777961758 0.9868886795559554 9 0.23877508991417026 0.9339018988304819 >10 1.6587963086505495 14.37545343488936 >50 0.22874204242592389 7.058019771387672 >100 0.21840379499488588 18.922325079562057 >500 0.014215078735694851 4.00055912848796 >1k 0.004165434831159433 2.9653485835512616 >5k 9.854557128216994E-5 0.2430800012999505 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 5676 0.24124601270409324 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3528 0.1499499529281256 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3302 0.1403443153539316 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3181 0.1352014739978366 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2832 0.1203679894252981 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2769 0.1176903116944387 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 2715 0.1153951593537021 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 2565 0.10901973618498927 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 2535 0.10774465155124671 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0043352877547247195 0.0 0.0 2 0.004717813144847489 0.0 0.0 3 0.005270349819469267 0.0 0.0 4 0.005652875209592036 0.0 0.0 5 0.006715445737710841 0.0 0.0 6 0.008203044477077166 0.0 0.0 7 0.009350620647445474 0.0 0.0 8 0.01066820810231279 0.0 0.0 9 0.012240812483928621 0.0 0.0 10-11 0.019317532201199854 0.0 0.0 12-13 0.026564263202970097 0.0 0.0 14-15 0.03257841239212253 0.0 0.0 16-17 0.038125030548902684 0.0 0.0 18-19 0.04377790575849472 0.0 0.0 20-21 0.05045084867508081 0.0 0.0 22-23 0.05648624927479562 0.0 0.0 24-25 0.06239414141113617 0.0 0.0 26-27 0.06959836959178164 0.0 0.0 28-29 0.07786516830054595 0.0 0.0 30-31 0.13290632165709998 0.0 0.0 32-33 0.14472210592978108 0.0 0.0 34-35 0.15396646952441467 0.0 0.0 36-37 0.16571849956540866 0.0 0.0 38-39 0.17887312270351946 0.0 0.0 40-41 0.19236776841062825 0.0 0.0 42-43 0.20979392507177663 0.0 0.0 44-45 0.22934522278916264 0.0 0.0 46-47 0.25010785090860405 0.0 0.0 48-49 0.2745469730553366 0.0 0.0 50-51 0.30091997356324524 0.0 0.0 52-53 0.3302044173181995 0.0 0.0 54-55 0.3602539118533993 0.0 0.0 56-57 0.39336360950958116 0.0 0.0 58-59 0.43238119930210367 0.0 0.0 60-61 0.4735664329719885 0.0 0.0 62-63 0.5180243838684793 0.0 0.0 64-65 0.5619510495009106 0.0 0.0 66-67 0.6089591696648865 0.0 0.0 68-69 0.6589424873075951 0.0 0.0 70-71 0.7137073723268382 0.0 0.0 72-73 0.7709374209713169 0.0 0.0 74-75 0.8329490369923304 0.0 0.0 76-77 0.8987221526828844 0.0 0.0 78-79 0.9685117849697273 0.0 0.0 80-81 1.0448468517097822 0.0 4.250282112475216E-5 82-83 1.131191332824716 0.0 4.250282112475216E-5 84-85 1.2220836157999988 0.0 4.250282112475216E-5 86-87 1.3182462485947504 0.0 4.250282112475216E-5 88-89 1.417171564762611 0.0 4.250282112475216E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1285 0.0 85.219734 9 CGGGAGA 2160 0.0 81.89586 4 CGGAAAG 1195 0.0 81.3566 5 TCGGAGA 480 0.0 81.01761 4 AGAGCGG 1440 0.0 80.65567 8 ATTCGGA 340 0.0 76.73641 2 AAGCGGC 650 0.0 75.84924 9 TCGGGAG 2245 0.0 75.423195 3 AACCGGA 340 0.0 75.34121 2 ACCGGAA 495 0.0 73.77998 3 GAGAGCG 2025 0.0 72.574905 7 GGATCGG 1050 0.0 71.843956 1 GAACCGG 385 0.0 71.47426 1 TCGGGAA 790 0.0 70.844795 3 GTTCGGA 330 0.0 70.436844 2 GAATCGG 510 0.0 67.91022 1 GGAAAGC 965 0.0 65.34758 6 AAGCGTC 320 0.0 65.18295 9 ATCGGGA 3200 0.0 63.298824 2 GATCGGG 3390 0.0 62.699024 1 >>END_MODULE