FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_34098-1.34200000035704.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_34098-1.34200000035704.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2352785
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT56760.24124601270409324Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA35280.1499499529281256No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG33020.1403443153539316No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC31810.1352014739978366No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG28320.1203679894252981No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC27690.1176903116944387No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC27150.1153951593537021No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT25650.10901973618498927No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT25350.10774465155124671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC12850.085.2197349
CGGGAGA21600.081.895864
CGGAAAG11950.081.35665
TCGGAGA4800.081.017614
AGAGCGG14400.080.655678
ATTCGGA3400.076.736412
AAGCGGC6500.075.849249
TCGGGAG22450.075.4231953
AACCGGA3400.075.341212
ACCGGAA4950.073.779983
GAGAGCG20250.072.5749057
GGATCGG10500.071.8439561
GAACCGG3850.071.474261
TCGGGAA7900.070.8447953
GTTCGGA3300.070.4368442
GAATCGG5100.067.910221
GGAAAGC9650.065.347586
AAGCGTC3200.065.182959
ATCGGGA32000.063.2988242
GATCGGG33900.062.6990241
Produced by FastQC (version 0.11.2)