FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_34042-1.34200000035847.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_34042-1.34200000035847.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5452897
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT278900.5114712418004594Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC93890.17218370345157813No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG90290.16558170821858548No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC82840.15191924586142008No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG82600.15147911284588725No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA55920.10255099261915271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC48550.090.9174359
ATTCGGA13200.089.857822
CGGGAGA66650.089.25234
GAGAGCG59500.087.4194957
AGAGCGG51750.087.3126758
CGGAAAG34050.087.073295
AAGCGGC19850.086.2250449
GTTCGGA13550.084.7355962
CGGAGAG23550.084.601955
TTCGGAG11900.082.521163
TCGGGAG74700.080.9713753
GGATCGG27800.080.91241
TCGGAGA18550.079.78714
AAAGCGG23850.079.716968
CCGGAGA10550.079.137484
GTCCGGA4950.078.5956342
AAGGGGC51600.078.376549
AAGCGTC8250.077.5839
GGAAAGC25800.077.381456
TCGGGAA24500.077.2589343
Produced by FastQC (version 0.11.2)