FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_34012-1.34200000035798.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_34012-1.34200000035798.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2889630
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT119830.41468976997054985Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC35800.1238912940411056No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG33250.11506663482868049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA40750.086.7449654
GAGCGGC32500.086.5199059
GAGAGCG37550.082.587157
TCGGGAG43000.082.544173
CGGAAAG20950.081.5317155
ATCGGGA53100.081.23962
GATCGGG54300.080.0685351
AAGCGGC13500.079.7327659
AAGCGTC4750.077.86569
GTTCGGA7700.077.656582
ATTCGGA7850.076.172692
TCGGAGA10550.073.757644
AGAGCGG40600.072.879148
TCGGGAA16500.072.471933
GAACCGG4600.072.228131
GGATCGG18650.071.768931
AAAGCGG16850.070.916188
GGTTCGG10000.070.2470251
GATCCGG9450.068.810541
ATCCGGA8850.068.1018452
Produced by FastQC (version 0.11.2)