##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_34012-1.34200000035798.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2889630 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.784206628530296 33.0 33.0 33.0 22.0 33.0 2 30.151806632683076 33.0 33.0 33.0 22.0 33.0 3 29.982708858919654 33.0 33.0 33.0 15.0 33.0 4 33.25752535791779 37.0 37.0 37.0 15.0 37.0 5 33.37702266380125 37.0 37.0 37.0 15.0 37.0 6 34.08025491152846 37.0 37.0 37.0 27.0 37.0 7 33.35861165616359 37.0 37.0 37.0 15.0 37.0 8 33.75139377705796 37.0 37.0 37.0 22.0 37.0 9 33.041456864719706 37.0 37.0 37.0 15.0 37.0 10-11 33.10152701210882 37.0 37.0 37.0 15.0 37.0 12-13 33.080563774600904 37.0 37.0 37.0 15.0 37.0 14-15 34.628335288600965 40.0 37.0 40.0 15.0 40.0 16-17 34.540490997117274 40.0 37.0 40.0 15.0 40.0 18-19 34.57948145610338 40.0 37.0 40.0 15.0 40.0 20-21 34.4826498548257 40.0 37.0 40.0 15.0 40.0 22-23 35.47579534404059 40.0 37.0 40.0 24.5 40.0 24-25 34.69516339462146 40.0 37.0 40.0 15.0 40.0 26-27 34.608722050920015 40.0 37.0 40.0 18.5 40.0 28-29 34.26161999979236 40.0 37.0 40.0 15.0 40.0 30-31 34.20755788803411 40.0 37.0 40.0 15.0 40.0 32-33 34.09600485183224 37.0 37.0 40.0 15.0 40.0 34-35 34.18154123538308 37.0 37.0 40.0 15.0 40.0 36-37 34.04292123905137 37.0 37.0 40.0 15.0 40.0 38-39 33.878564037610346 37.0 37.0 40.0 15.0 40.0 40-41 33.91313697601423 37.0 35.0 40.0 15.0 40.0 42-43 33.776947740714206 37.0 33.0 40.0 15.0 40.0 44-45 33.5546616694871 37.0 33.0 40.0 10.5 40.0 46-47 33.47877184968318 37.0 33.0 40.0 10.5 40.0 48-49 33.381388966753526 37.0 33.0 40.0 10.5 40.0 50-51 32.47278129033821 37.0 33.0 38.5 15.0 38.5 52-53 32.86121285424086 37.0 33.0 38.5 15.0 40.0 54-55 33.47629869568076 37.0 33.0 40.0 10.5 40.0 56-57 33.82735782781879 37.0 33.0 40.0 18.5 40.0 58-59 33.208316289628776 37.0 33.0 40.0 6.0 40.0 60-61 33.033401335119024 37.0 33.0 40.0 10.5 40.0 62-63 32.64317109802985 37.0 33.0 40.0 6.0 40.0 64-65 32.49248381280648 37.0 33.0 40.0 6.0 40.0 66-67 32.44412848703813 37.0 33.0 37.0 10.5 40.0 68-69 32.61949263400505 37.0 33.0 37.0 15.0 40.0 70-71 32.14238051238394 37.0 33.0 37.0 15.0 40.0 72-73 32.5347734831103 37.0 33.0 37.0 18.5 40.0 74-75 32.70484404577749 37.0 33.0 37.0 22.0 37.0 76-77 32.39149597007229 37.0 33.0 37.0 22.0 37.0 78-79 31.846839906839286 37.0 33.0 37.0 18.5 37.0 80-81 31.281786768548223 37.0 33.0 37.0 15.0 37.0 82-83 30.884913466429957 37.0 33.0 37.0 15.0 37.0 84-85 30.643899738028743 33.0 30.0 37.0 15.0 37.0 86-87 30.469269595069264 33.0 27.0 37.0 15.0 37.0 88-89 30.31069012295692 33.0 27.0 37.0 6.0 37.0 90-91 30.14161518948793 33.0 27.0 37.0 6.0 37.0 92-93 29.99097825673183 33.0 27.0 37.0 6.0 37.0 94-95 29.881701117444102 33.0 27.0 37.0 6.0 37.0 96-97 29.753010073954105 33.0 27.0 37.0 6.0 37.0 98-99 29.618388859473356 33.0 27.0 37.0 6.0 37.0 100-101 28.47354315258355 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.011738103715693171 2306 2 0.12287658024852632 2306 3 0.0614522180403263 2306 4 0.30818831258561374 2306 5 0.14764145371745485 2306 6 -0.2952208720979428 2306 7 0.43253351250941563 2306 8 -0.25149510246585294 2306 9 0.22211365485723888 2306 10-11 0.12125764121056193 2306 12-13 0.1572886235872275 2306 14-15 0.4505276092902406 2306 16-17 0.39573362653136 2306 18-19 0.4748601679911175 2306 20-21 0.481701355726905 2306 22-23 -0.055836605346677004 2306 24-25 0.2752688028686805 2306 26-27 -0.03860900476511375 2306 28-29 0.2424318823448104 2306 30-31 0.3282179934043725 2306 32-33 0.2877118117167825 2306 34-35 0.23844851091165253 2306 36-37 0.10157663512891446 2306 38-39 0.10517651755576907 2306 40-41 0.12694425415713084 2306 42-43 0.21724171691143823 2306 44-45 0.22531977500114664 2306 46-47 0.2225259739230978 2306 48-49 0.12634817215896277 2306 50-51 0.08092635063654541 2306 52-53 0.03491581870140692 2306 54-55 0.21774564858210255 2306 56-57 0.021486581176240804 2306 58-59 0.44163351251904004 2306 60-61 0.35241668245021174 2306 62-63 0.41078085784266705 2306 64-65 0.37848579650822245 2306 66-67 0.18139969995076655 2306 68-69 -0.03322264876690184 2306 70-71 0.1800651072739612 2306 72-73 -0.26153115124878923 2306 74-75 -0.3463052430002662 2306 76-77 -0.10384222041170688 2306 78-79 0.08440282181875247 2306 80-81 0.3100468790959745 2306 82-83 0.2651872495379344 2306 84-85 0.43500094911239273 2306 86-87 0.4024028802023345 2306 88-89 0.3570931314854775 2306 90-91 0.2548408232182311 2306 92-93 0.39586467784952717 2306 94-95 0.43482221266531695 2306 96-97 0.2729337087502799 2306 98-99 0.23088411340020087 2306 100-101 -0.17956992313080278 2307 1 -0.14192159745002186 2307 2 -0.07623942661661331 2307 3 -0.10880526523527578 2307 4 0.07743650278200676 2307 5 0.007383777828671612 2307 6 -0.2551670620571187 2307 7 0.2109034575538331 2307 8 -0.31536887797823 2307 9 -0.010920114288765603 2307 10-11 0.0018094881175443334 2307 12-13 -0.038630004439205834 2307 14-15 0.20619302194371159 2307 16-17 0.1833870201222041 2307 18-19 0.28706424064481695 2307 20-21 0.29184497419871036 2307 22-23 -0.08753090186757362 2307 24-25 0.09487337814140062 2307 26-27 -0.20460781056337396 2307 28-29 0.0939854090415011 2307 30-31 0.06571562375534512 2307 32-33 0.08125693845119031 2307 34-35 -0.02715199109913158 2307 36-37 -0.11370782919862421 2307 38-39 -0.13307270971055374 2307 40-41 -0.15069994468860415 2307 42-43 -0.08263359139638027 2307 44-45 0.021035652308576402 2307 46-47 -0.002054306744724954 2307 48-49 -0.16015156984075674 2307 50-51 -0.1936489119509801 2307 52-53 -0.20383288496118723 2307 54-55 -0.11748563423183356 2307 56-57 -0.22079718264755144 2307 58-59 0.01588224507360536 2307 60-61 -0.051312308773717064 2307 62-63 -0.06499256011881016 2307 64-65 -0.05875505005713677 2307 66-67 -0.2085748852466125 2307 68-69 -0.3017138691538932 2307 70-71 -0.1706952586099888 2307 72-73 -0.3874362051145823 2307 74-75 -0.38381584939725855 2307 76-77 -0.17303100464743437 2307 78-79 0.13298240096908032 2307 80-81 0.26285158047964785 2307 82-83 0.16306394263315838 2307 84-85 -0.01527210541842905 2307 86-87 0.1464170200374788 2307 88-89 0.26956757222296446 2307 90-91 0.15446340275480708 2307 92-93 0.10146323345340491 2307 94-95 0.1802238770459681 2307 96-97 0.23886976320100928 2307 98-99 0.22074667882532495 2307 100-101 0.08040282717256275 2201 1 -0.02574763470824948 2201 2 -0.05692231672466619 2201 3 -0.11672609015183966 2201 4 0.13057513785022223 2201 5 0.009568473926456988 2201 6 -0.11609389768389633 2201 7 0.354857961884278 2201 8 -0.19912087081443275 2201 9 0.08327518867247363 2201 10-11 0.045174452302411794 2201 12-13 0.09363965377637129 2201 14-15 0.2906510977605734 2201 16-17 0.3091315136467898 2201 18-19 0.3540933393313779 2201 20-21 0.3394567909171684 2201 22-23 0.2439814978735697 2201 24-25 0.30707803923776567 2201 26-27 -0.04594488314002376 2201 28-29 0.11131026120332876 2201 30-31 0.21960548190629225 2201 32-33 0.17158843378600608 2201 34-35 0.19491773946401736 2201 36-37 0.06716794277232196 2201 38-39 0.05831110340749035 2201 40-41 0.08438396075357701 2201 42-43 0.1413340095412039 2201 44-45 0.15043821023483872 2201 46-47 0.169329703053819 2201 48-49 0.1266361671688898 2201 50-51 0.13073488034207514 2201 52-53 0.06200452274360657 2201 54-55 0.226420239881854 2201 56-57 0.37145647888767996 2201 58-59 0.3828877613830457 2201 60-61 0.18256239726606793 2201 62-63 0.33633158167933885 2201 64-65 0.23333631150409673 2201 66-67 0.19231627022431041 2201 68-69 0.12975666849345657 2201 70-71 0.17185024207299193 2201 72-73 -0.059901186345094004 2201 74-75 0.136873203366342 2201 76-77 0.3282643870899804 2201 78-79 0.2995690456692408 2201 80-81 0.37706789740211377 2201 82-83 0.2230492764385268 2201 84-85 0.38898807125464785 2201 86-87 0.485090807309394 2201 88-89 0.4447130965076056 2201 90-91 0.27274734228828024 2201 92-93 0.3415530431669538 2201 94-95 0.37786049093284646 2201 96-97 0.4795210764482505 2201 98-99 0.3255256099478139 2201 100-101 0.3223001692091998 1101 1 -0.1369924037410648 1101 2 -0.22323576264550837 1101 3 -0.04354712442976094 1101 4 -0.044881524448122434 1101 5 -0.09718663669612937 1101 6 -0.26698994023551137 1101 7 0.23560817756000318 1101 8 -0.24042303442852386 1101 9 0.0103750446087858 1101 10-11 -0.0028440947603769473 1101 12-13 0.03278455287931337 1101 14-15 0.20307730797600954 1101 16-17 0.10840344627619203 1101 18-19 0.18064072956764932 1101 20-21 0.1633629843963078 1101 22-23 -0.0824213214087095 1101 24-25 0.07080091010030998 1101 26-27 -0.06547779214200489 1101 28-29 0.05235469844886609 1101 30-31 0.23580101460259328 1101 32-33 0.1366919966160367 1101 34-35 0.03519534718117967 1101 36-37 -0.040211368030909966 1101 38-39 0.08472237130087734 1101 40-41 -0.003675580439612247 1101 42-43 0.12550397110789646 1101 44-45 0.1339138289075663 1101 46-47 0.0741284616852198 1101 48-49 0.10996896408246215 1101 50-51 0.24349340259428232 1101 52-53 0.1695539427455799 1101 54-55 0.2833131520743706 1101 56-57 0.27926525200941654 1101 58-59 0.441788836718068 1101 60-61 0.2884862904980139 1101 62-63 0.23999633729232528 1101 64-65 0.26218963032144416 1101 66-67 0.251064028631113 1101 68-69 0.21856661427541013 1101 70-71 0.4019160677734206 1101 72-73 0.044195060652413076 1101 74-75 0.25588299815779436 1101 76-77 0.3428306322473347 1101 78-79 0.4188772531013534 1101 80-81 0.40725279079568466 1101 82-83 0.29333201279498056 1101 84-85 0.3465151715303918 1101 86-87 0.5074969437647816 1101 88-89 0.3554478353073378 1101 90-91 0.4592401121663059 1101 92-93 0.4477756988785302 1101 94-95 0.3221917527530316 1101 96-97 0.4119297387409979 1101 98-99 0.4097401584911218 1101 100-101 0.2255399579344477 2304 1 0.06706238785603702 2304 2 0.044736148278055055 2304 3 0.21609645629003893 2304 4 0.25455403568241053 2304 5 0.13519375231807373 2304 6 0.04241005489448213 2304 7 0.6101900976481716 2304 8 -0.03734829271331108 2304 9 0.2935418387382853 2304 10-11 0.28756574724205564 2304 12-13 0.27326799555772396 2304 14-15 0.5431863340663696 2304 16-17 0.4324200502040654 2304 18-19 0.5416558838157712 2304 20-21 0.41400007885783197 2304 22-23 0.14890813897759614 2304 24-25 0.32659865969236535 2304 26-27 0.244099399852999 2304 28-29 0.27164252680008616 2304 30-31 0.2831525922595617 2304 32-33 0.34267152615176855 2304 34-35 0.13798627923048912 2304 36-37 0.2027348396537434 2304 38-39 0.24663183851449588 2304 40-41 0.3038589233184368 2304 42-43 0.1601885707693853 2304 44-45 0.28054613463349654 2304 46-47 0.16537437820866785 2304 48-49 0.32367900735262367 2304 50-51 0.1920554403411714 2304 52-53 0.19699890664006148 2304 54-55 0.32843158394492633 2304 56-57 0.4099884587189777 2304 58-59 0.4839918567109933 2304 60-61 0.4059418334718572 2304 62-63 0.5228139333985524 2304 64-65 0.39089814357227937 2304 66-67 0.3584783642219733 2304 68-69 0.25808097233963423 2304 70-71 0.3881040180453894 2304 72-73 0.16155514220795197 2304 74-75 0.30336803568552995 2304 76-77 0.33592685627132113 2304 78-79 0.48759582586087546 2304 80-81 0.49971326193617216 2304 82-83 0.4326257316818065 2304 84-85 0.29014198578201444 2304 86-87 0.383285198396603 2304 88-89 0.5354869070222037 2304 90-91 0.42742563643095366 2304 92-93 0.4020987673047465 2304 94-95 0.3894038807423641 2304 96-97 0.28454008198179537 2304 98-99 0.3923859550635349 2304 100-101 0.30465521747706603 2202 1 -0.15443178747023723 2202 2 -0.374826999376932 2202 3 -0.18615469059805534 2202 4 -0.2277771700588218 2202 5 -0.17096803424927032 2202 6 -0.37493288298365 2202 7 0.047851019646707016 2202 8 -0.3942460702522439 2202 9 -0.19575820548870837 2202 10-11 -0.12524519432819403 2202 12-13 -0.11480037400568222 2202 14-15 7.099708181996789E-4 2202 16-17 -0.013756493220093091 2202 18-19 -0.043569757384908314 2202 20-21 -0.029751895561680897 2202 22-23 -0.10679405719419321 2202 24-25 -0.29216491614386086 2202 26-27 -0.2568051112280756 2202 28-29 -0.1226012097057847 2202 30-31 -0.08240634184802076 2202 32-33 -0.017478279184082623 2202 34-35 -0.3509723674379259 2202 36-37 -0.11896928681949248 2202 38-39 -0.09999075124125056 2202 40-41 -0.16844568086445832 2202 42-43 -0.1533698724112682 2202 44-45 -0.08325875241337144 2202 46-47 -0.1446424919578604 2202 48-49 -0.21553211083512736 2202 50-51 -0.001128155437150724 2202 52-53 -0.17609735352796463 2202 54-55 0.017752107668101758 2202 56-57 -0.08089983579650806 2202 58-59 -0.013546028541377098 2202 60-61 0.037565951927589936 2202 62-63 0.09635384336139907 2202 64-65 -0.06508030793089148 2202 66-67 0.01034544793445491 2202 68-69 -0.0259294794656455 2202 70-71 -0.04153838235490781 2202 72-73 -0.05625192496116682 2202 74-75 0.09015069957415278 2202 76-77 0.12028720244743596 2202 78-79 0.2270685296699888 2202 80-81 0.23651535229722498 2202 82-83 0.14699631034376992 2202 84-85 0.1667473567840645 2202 86-87 0.0900057411468218 2202 88-89 0.1971071692323747 2202 90-91 0.1851692462108545 2202 92-93 0.11455727272273819 2202 94-95 0.13193500122498492 2202 96-97 0.13015518219697242 2202 98-99 0.15735301720718908 2202 100-101 -0.04689254586420333 1102 1 -0.16755395726909228 1102 2 -0.13198260867378764 1102 3 -0.07784314259791358 1102 4 -0.003948613231806064 1102 5 -0.15153142770492423 1102 6 -0.2665606049076885 1102 7 0.1959611010426272 1102 8 -0.20299052337993828 1102 9 -0.09277978838831302 1102 10-11 -0.012948167322960558 1102 12-13 -0.08662371997530016 1102 14-15 -0.018118250502254796 1102 16-17 -0.07829785575628279 1102 18-19 0.006539536417854208 1102 20-21 -0.00563026189553284 1102 22-23 -0.21132346349669717 1102 24-25 -0.258814412104563 1102 26-27 -0.33730025112753026 1102 28-29 -0.16422876218205573 1102 30-31 -0.15760983165845488 1102 32-33 -0.18916186464438312 1102 34-35 -0.24103662880498433 1102 36-37 -0.08959368824529435 1102 38-39 -0.06665603973284107 1102 40-41 -0.025348474109058827 1102 42-43 -0.10568883273405305 1102 44-45 0.03705478015889696 1102 46-47 -0.1655600718914485 1102 48-49 -0.16108847898681944 1102 50-51 -0.17477286144868032 1102 52-53 -0.1324600828839806 1102 54-55 0.045039499873915645 1102 56-57 0.1789079020013844 1102 58-59 0.11818645276346018 1102 60-61 0.09213348397524612 1102 62-63 0.13849312806141967 1102 64-65 0.04558988505655037 1102 66-67 0.013748636670108283 1102 68-69 -0.10049286343222974 1102 70-71 -0.04561665095128831 1102 72-73 -0.1456000323314015 1102 74-75 -0.08800396013747758 1102 76-77 0.11580835935537692 1102 78-79 0.15860255376274424 1102 80-81 -0.032193340576004204 1102 82-83 0.06417905839325755 1102 84-85 0.06308965751858508 1102 86-87 0.15002447529074914 1102 88-89 0.19427671391269996 1102 90-91 0.1415416679785224 1102 92-93 0.10847253935555656 1102 94-95 0.09492246005765281 1102 96-97 0.15767485338699316 1102 98-99 0.09386494192358086 1102 100-101 0.025668440077733834 2305 1 -0.1267327127098774 2305 2 -0.15332176973305778 2305 3 -0.14517915507281742 2305 4 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-0.5786207879139269 1213 70-71 -1.6790084524359514 1213 72-73 -0.6946275521821548 1213 74-75 -0.8517436771256399 1213 76-77 -0.8872624857101101 1213 78-79 -1.0361559448373505 1213 80-81 -1.3291442899858197 1213 82-83 -1.4132694272533648 1213 84-85 -1.408935289552911 1213 86-87 -1.3260230118473224 1213 88-89 -1.4435041313651382 1213 90-91 -1.4310068009158954 1213 92-93 -1.4153627866925014 1213 94-95 -1.4641978942956975 1213 96-97 -1.418865613596779 1213 98-99 -1.3429829442613688 1213 100-101 -1.1903644026410447 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 273.0 3 242.0 4 350.0 5 242.0 6 737.0 7 4067.0 8 20012.0 9 68530.0 10 93129.0 11 63669.0 12 33857.0 13 21302.0 14 14857.0 15 11585.0 16 10233.0 17 9880.0 18 9924.0 19 9935.0 20 10462.0 21 11130.0 22 11676.0 23 12661.0 24 13478.0 25 15249.0 26 17892.0 27 21355.0 28 26737.0 29 32898.0 30 39135.0 31 49067.0 32 66434.0 33 95558.0 34 143823.0 35 242239.0 36 486374.0 37 901247.0 38 318892.0 39 499.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.009577464788734 22.075562296858074 23.588749729144094 13.326110509209101 2 31.03802469067326 26.364913341366652 31.091376013467137 11.505685954492952 3 36.17751071604829 19.867699085600847 29.54180922384203 14.412980974508836 4 27.095549872938168 17.700667763375627 35.696733057180765 19.507049306505433 5 34.87290333916599 16.106084219580723 32.08598497096325 16.93502747029005 6 31.222780430218627 16.354385501699397 37.59629607684767 14.826537991234298 7 36.12291218064366 19.024513431048963 28.750765568405097 16.101808819902285 8 27.154271273935386 33.005616615428096 24.387180705665244 15.452931404971274 9 33.41374820711777 27.55869102365652 22.63754320495471 16.390017564270995 10-11 26.923236753771462 30.755962157738885 26.702037732137768 15.618763356351884 12-13 29.548858403836654 23.807764752010495 26.684424983149167 19.95895186100368 14-15 25.608935551957412 24.26777836874427 29.083339380588985 21.03994669870933 16-17 27.42669338916125 25.252255165072246 30.14064984290173 17.180401602864777 18-19 27.403474751364982 25.381734480258384 30.590727127231897 16.624063641144737 20-21 25.36107378366397 28.78861659093428 29.202472154494068 16.64783747090768 22-23 29.380359181996596 25.896651649511337 27.996322583807487 16.72666658468458 24-25 24.406322639985845 30.812093967789604 27.928898109096544 16.852685283128004 26-27 22.337883174379535 33.087075680979595 27.810116072567258 16.76492507207361 28-29 24.387270358486774 30.84332402408854 27.675293310510668 17.09411230691402 30-31 27.69909529063071 27.04928908259317 28.05781898146614 17.193796645309977 32-33 27.23614719992894 26.74377591867234 29.03203588238121 16.98804099901751 34-35 22.84092300743084 26.794803165504867 28.32919391059055 22.035079916473745 36-37 19.24993078621997 27.00452821181429 29.4037137442955 24.34182725767024 38-39 19.28765054596987 28.987325476326966 31.3908386435907 20.334185334112465 40-41 21.38981783418825 26.85959812308229 33.89158536045825 17.858998682271213 42-43 23.09840935091188 26.380988709820734 33.00006802985539 17.520533909412002 44-45 21.129854809642065 28.876434730964682 32.57990709524377 17.41380336414948 46-47 23.840643227410048 30.46898007285454 28.269009608948707 17.4213670907867 48-49 20.182353976103716 29.523531744943877 30.936741225587788 19.357373053364626 50-51 19.228472537360314 27.160754989401237 32.80441616565133 20.80635630758712 52-53 25.63822105560724 26.59118607572597 28.129465803366955 19.64112706529984 54-55 27.536961555263552 26.750453941230006 27.86989227201706 17.842692231489384 56-57 28.366585574924258 26.53324125198247 27.338530839733334 17.76164233335994 58-59 26.00444372113585 26.519240104947606 27.837678216432053 19.63863795748449 60-61 21.178106214814704 26.73694691167326 27.47137691749284 24.613569956019195 62-63 24.402617369157557 26.752220628831857 27.702999435953686 21.1421625660569 64-65 20.96750023414817 28.73752510588701 30.7226853152675 19.572289344697325 66-67 19.246775557724956 28.764977377386547 29.11838416049324 22.869862904395262 68-69 18.808567092865683 27.282750562209397 35.037476000718854 18.87120634420607 70-71 18.333796719710097 32.215476573086896 31.36242359111318 18.088303116089822 72-73 18.248528115134103 36.27570985883641 27.1425489362263 18.333213089803188 74-75 18.67237535090283 36.54029105964199 26.574416549518382 18.212917039936798 76-77 18.91973411967251 36.06916440708628 26.787566141833487 18.223535331407724 78-79 19.16185934099557 35.586860290637155 26.999758730328328 18.25152163803895 80-81 18.63509798588426 35.699434787556164 26.928633082343666 18.736834144215905 82-83 18.4846495805509 34.99199169655791 27.106116136796505 19.417242586094684 84-85 18.715279928421936 33.85613287953368 27.70357007219398 19.725017119850406 86-87 18.817363526697655 33.56550723717392 28.017744595866983 19.599384640261437 88-89 19.221171677664163 33.8697362145593 27.485886699067247 19.423205408709293 90-91 19.80691028173605 33.143760624540384 27.083719686827713 19.965609406895847 92-93 19.58069332104931 33.08932948255181 27.38532466514365 19.944652531255226 94-95 19.09813817563759 33.17474440021853 27.480805085113207 20.246312339030673 96-97 19.505576121142436 33.639883422171465 27.043229354997873 19.81131110168823 98-99 19.943115771513163 32.87890018736477 26.946700178725973 20.231283862396094 100-101 20.142385809786546 32.73820573130902 26.425219952541536 20.6941885063629 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 148.0 1 176.0 2 206.0 3 255.5 4 307.0 5 317.5 6 377.5 7 526.0 8 706.5 9 864.0 10 1043.5 11 1152.0 12 1196.5 13 1201.5 14 1098.0 15 1040.5 16 998.5 17 975.0 18 1015.5 19 1137.5 20 1295.0 21 1558.0 22 2056.5 23 2829.0 24 3919.5 25 5496.0 26 8314.5 27 12484.0 28 16899.5 29 21372.0 30 25888.5 31 30467.0 32 37466.5 33 48893.5 34 65351.5 35 88468.0 36 114078.5 37 142631.0 38 175059.0 39 190466.0 40 185216.0 41 170367.5 42 156104.0 43 153586.0 44 148081.5 45 140707.0 46 143187.5 47 135178.5 48 112360.5 49 93401.0 50 80864.0 51 69855.5 52 60067.0 53 51160.0 54 42707.5 55 34267.0 56 26305.5 57 19793.0 58 14625.0 59 11109.5 60 8432.0 61 6150.5 62 4418.5 63 3136.5 64 2205.5 65 1582.0 66 1167.0 67 845.0 68 619.5 69 460.0 70 357.0 71 300.5 72 228.0 73 164.5 74 148.0 75 123.5 76 103.0 77 93.0 78 73.0 79 64.5 80 55.5 81 44.0 82 40.5 83 33.5 84 22.5 85 22.5 86 27.0 87 18.5 88 12.5 89 11.5 90 11.0 91 10.5 92 7.5 93 8.5 94 10.5 95 9.0 96 5.5 97 1.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18185719278938825 2 0.1722711904292245 3 0.14666237545983396 4 0.13936040254288612 5 0.14358239636216402 6 0.124894882735852 7 0.09987437838062312 8 0.08589334966760451 9 0.08717379041607404 10-11 0.06779414665545416 12-13 0.08762367500337413 14-15 0.0915515135155712 16-17 0.08390347553146943 18-19 0.08013136629949164 20-21 0.07855677024394127 22-23 0.09760765219076491 24-25 0.4449358568398031 26-27 0.27780373265781433 28-29 0.12492948924256739 30-31 0.2343033537165658 32-33 0.2650339316798344 34-35 0.24857853773666527 36-37 0.2511048127268889 38-39 0.26944626128604704 40-41 0.26915210597896616 42-43 0.29560878036288385 44-45 0.2720590525430592 46-47 0.2910234182230943 48-49 0.27432577873291736 50-51 0.2906081401425096 52-53 0.2892930928873247 54-55 0.2647051698660382 56-57 0.2810048345289881 58-59 0.2703114239539318 60-61 0.246484844080384 62-63 0.23836961825562442 64-65 0.23670850593328555 66-67 0.24625990178673393 68-69 0.25096638670002736 70-71 0.23762557836124348 72-73 0.2281260922678682 74-75 0.23835231500226672 76-77 0.2389406256164284 78-79 0.24098240951263655 80-81 0.2482670791762267 82-83 0.2745507210265674 84-85 0.26790627173721204 86-87 0.26780245221706583 88-89 0.26596830736115007 90-91 0.2576973522561712 92-93 0.23366313334233102 94-95 0.23203662752670756 96-97 0.22762429792049502 98-99 0.2520910981682776 100-101 0.2606216020736219 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2889630.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.69777794131771 #Duplication Level Percentage of deduplicated Percentage of total 1 92.33683853670753 56.969777598410886 2 3.7038846858849928 4.570429097399592 3 1.2834323960716134 2.3755478092655915 4 0.48906146894349195 1.2069602364212086 5 0.3654611477770455 1.1274070370863627 6 0.27041809170132686 1.001051722386201 7 0.18624658896239654 0.8043700481690871 8 0.13955574209762822 0.6888223349100216 9 0.12002345275125566 0.666466230203749 >10 0.9176894860185605 10.827999849859617 >50 0.10399776092627516 4.350416399504434 >100 0.07286730067564125 9.602303986689652 >500 0.00846240348537095 3.470386840038568 >1k 0.0020048895402513035 1.9236808531846963 >5k 0.0 0.0 >10k+ 5.60484566064971E-5 0.4143799564703063 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 11983 0.41468976997054985 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3580 0.1238912940411056 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3325 0.11506663482868049 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004706484913293397 0.0 0.0 2 0.005329402034170465 0.0 0.0 3 0.005917712648332139 0.0 0.0 4 0.006298384222201458 0.0 0.0 5 0.007544218463955593 0.0 0.0 6 0.008409381131840409 0.0 0.0 7 0.009828247907171506 0.0 0.0 8 0.01117790166907182 0.0 0.0 9 0.012458342417541346 0.0 0.0 10-11 0.01967379906770071 0.0 0.0 12-13 0.02479556206157882 0.0 1.7303253357696314E-5 14-15 0.03047102916290321 0.0 3.460650671539263E-5 16-17 0.03502178479597734 0.0 3.460650671539263E-5 18-19 0.03908804933503597 0.0 3.460650671539263E-5 20-21 0.04433093510241796 0.0 3.460650671539263E-5 22-23 0.04964303388323073 0.0 3.460650671539263E-5 24-25 0.054799403383824226 0.0 3.460650671539263E-5 26-27 0.06063059976536789 0.0 3.460650671539263E-5 28-29 0.06836515401625815 0.0 3.460650671539263E-5 30-31 0.13622851368514308 0.0 3.460650671539263E-5 32-33 0.14747562836764566 0.0 3.460650671539263E-5 34-35 0.15572928021926682 0.0 3.460650671539263E-5 36-37 0.16488270124548818 0.0 3.460650671539263E-5 38-39 0.1749531946996674 0.0 3.460650671539263E-5 40-41 0.18609648986202387 0.0 3.460650671539263E-5 42-43 0.19775888262511118 3.460650671539263E-5 3.460650671539263E-5 44-45 0.21182642760491827 3.460650671539263E-5 5.190976007308894E-5 46-47 0.2263438571720255 3.460650671539263E-5 6.921301343078525E-5 48-49 0.24193408844730985 3.460650671539263E-5 6.921301343078525E-5 50-51 0.2587701539643484 3.460650671539263E-5 6.921301343078525E-5 52-53 0.2770942992701488 3.460650671539263E-5 6.921301343078525E-5 54-55 0.29547035433602226 3.460650671539263E-5 6.921301343078525E-5 56-57 0.3164072908988348 3.460650671539263E-5 6.921301343078525E-5 58-59 0.33955904389143243 3.460650671539263E-5 6.921301343078525E-5 60-61 0.36406045064593046 3.460650671539263E-5 6.921301343078525E-5 62-63 0.3911054356440098 3.460650671539263E-5 6.921301343078525E-5 64-65 0.41892906704318544 3.460650671539263E-5 6.921301343078525E-5 66-67 0.44957312873966565 3.460650671539263E-5 6.921301343078525E-5 68-69 0.48277807193308486 3.460650671539263E-5 6.921301343078525E-5 70-71 0.5197205178517665 3.460650671539263E-5 8.651626678848158E-5 72-73 0.5593968778009641 3.460650671539263E-5 1.0381952014617788E-4 74-75 0.6019109713008239 3.460650671539263E-5 1.0381952014617788E-4 76-77 0.6453940469887148 3.460650671539263E-5 1.384260268615705E-4 78-79 0.6946564092980763 3.460650671539263E-5 1.384260268615705E-4 80-81 0.7493000834016812 3.460650671539263E-5 1.384260268615705E-4 82-83 0.8091001270058796 3.460650671539263E-5 1.384260268615705E-4 84-85 0.8745237279513294 3.460650671539263E-5 1.384260268615705E-4 86-87 0.9446192073033572 3.460650671539263E-5 1.384260268615705E-4 88-89 1.0208400383440095 3.460650671539263E-5 1.384260268615705E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGA 4075 0.0 86.744965 4 GAGCGGC 3250 0.0 86.519905 9 GAGAGCG 3755 0.0 82.58715 7 TCGGGAG 4300 0.0 82.54417 3 CGGAAAG 2095 0.0 81.531715 5 ATCGGGA 5310 0.0 81.2396 2 GATCGGG 5430 0.0 80.068535 1 AAGCGGC 1350 0.0 79.732765 9 AAGCGTC 475 0.0 77.8656 9 GTTCGGA 770 0.0 77.65658 2 ATTCGGA 785 0.0 76.17269 2 TCGGAGA 1055 0.0 73.75764 4 AGAGCGG 4060 0.0 72.87914 8 TCGGGAA 1650 0.0 72.47193 3 GAACCGG 460 0.0 72.22813 1 GGATCGG 1865 0.0 71.76893 1 AAAGCGG 1685 0.0 70.91618 8 GGTTCGG 1000 0.0 70.247025 1 GATCCGG 945 0.0 68.81054 1 ATCCGGA 885 0.0 68.101845 2 >>END_MODULE