##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_30749-1.3420000003587d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4991489 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.608418850567435 33.0 27.0 33.0 15.0 33.0 2 26.88218325233212 33.0 22.0 33.0 6.0 33.0 3 26.41567015373569 33.0 22.0 33.0 6.0 33.0 4 28.879929816533704 37.0 15.0 37.0 6.0 37.0 5 29.132642584206838 37.0 15.0 37.0 6.0 37.0 6 31.17735289008951 37.0 27.0 37.0 15.0 37.0 7 29.240351927050224 37.0 22.0 37.0 6.0 37.0 8 30.260214136503155 37.0 27.0 37.0 6.0 37.0 9 28.551751992241194 37.0 15.0 37.0 6.0 37.0 10-11 28.788004641500763 37.0 15.0 37.0 6.0 37.0 12-13 28.785589229987284 37.0 15.0 37.0 6.0 37.0 14-15 29.874913678062796 37.0 15.0 40.0 6.0 40.0 16-17 29.661213818161272 37.0 15.0 40.0 6.0 40.0 18-19 29.826141758501322 37.0 15.0 40.0 6.0 40.0 20-21 29.733409108985313 37.0 15.0 40.0 6.0 40.0 22-23 32.538166066278016 37.0 27.0 40.0 18.5 40.0 24-25 30.631490222657007 37.0 22.0 40.0 6.0 40.0 26-27 30.392614207904696 37.0 21.0 40.0 10.5 40.0 28-29 29.49639766811066 37.0 15.0 40.0 6.0 40.0 30-31 29.42469150988813 37.0 15.0 40.0 6.0 40.0 32-33 29.195531633947304 37.0 15.0 40.0 6.0 40.0 34-35 29.39948079621131 37.0 15.0 40.0 6.0 40.0 36-37 29.035305797528554 37.0 15.0 40.0 6.0 40.0 38-39 29.03800899891796 37.0 15.0 40.0 6.0 40.0 40-41 29.30703453418409 37.0 15.0 40.0 6.0 40.0 42-43 28.476954471902072 37.0 10.5 40.0 6.0 40.0 44-45 28.157961081352678 37.0 6.0 40.0 6.0 40.0 46-47 28.21470126449242 37.0 10.5 40.0 6.0 40.0 48-49 28.17808353379122 37.0 10.5 40.0 6.0 40.0 50-51 27.692352622634246 35.0 15.0 38.5 6.0 38.5 52-53 28.104175928265093 37.0 15.0 37.0 6.0 40.0 54-55 28.47865877296334 37.0 15.0 40.0 6.0 40.0 56-57 29.155778766616535 37.0 18.5 40.0 6.0 40.0 58-59 27.99773814987872 37.0 10.5 40.0 6.0 40.0 60-61 27.70839843581745 37.0 10.5 38.5 6.0 40.0 62-63 27.299774876795283 37.0 6.0 37.0 6.0 40.0 64-65 27.135818690575096 37.0 6.0 37.0 6.0 40.0 66-67 27.315958123918534 37.0 10.5 37.0 6.0 40.0 68-69 27.948012707230248 35.0 15.0 37.0 6.0 40.0 70-71 27.404138023744018 33.0 15.0 37.0 6.0 40.0 72-73 28.41432897077405 33.0 18.5 37.0 6.0 37.0 74-75 29.284345212420583 33.0 22.0 37.0 15.0 37.0 76-77 29.05060233529514 33.0 22.0 37.0 15.0 37.0 78-79 28.34316523586449 33.0 22.0 37.0 6.0 37.0 80-81 27.56600825925891 33.0 18.5 37.0 6.0 37.0 82-83 26.958043181102873 33.0 15.0 37.0 6.0 37.0 84-85 26.586099558668764 33.0 15.0 37.0 6.0 37.0 86-87 26.33344829568892 33.0 15.0 37.0 6.0 37.0 88-89 26.111144189639603 33.0 15.0 37.0 6.0 37.0 90-91 25.91480758547199 33.0 15.0 37.0 6.0 37.0 92-93 25.768485916727453 33.0 15.0 37.0 6.0 37.0 94-95 25.646177423209785 33.0 15.0 37.0 6.0 37.0 96-97 25.506003118508325 33.0 15.0 37.0 6.0 37.0 98-99 25.388025997853546 33.0 15.0 37.0 6.0 37.0 100-101 24.40929710553304 30.0 15.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -1.0032337947570689 2306 2 -0.9370415319451588 2306 3 -1.0508805728440862 2306 4 -0.684742743880804 2306 5 -0.872657759653066 2306 6 -1.9008920457289236 2306 7 -0.006376619432433728 2306 8 -1.7726212105758208 2306 9 -0.7357000930314861 2306 10-11 -0.8437729843172015 2306 12-13 -0.913551191186631 2306 14-15 -0.2053061680524415 2306 16-17 -0.5387541482470795 2306 18-19 -0.4413426807042953 2306 20-21 -0.4819598159591685 2306 22-23 -1.2490769238375918 2306 24-25 -0.18748098528005386 2306 26-27 -1.6042957668935856 2306 28-29 -1.0503261179739702 2306 30-31 -0.6600914583535911 2306 32-33 -0.7083119970328475 2306 34-35 -0.9672443039749545 2306 36-37 -0.7297739266683401 2306 38-39 -0.7151416621144335 2306 40-41 -1.1912530224396605 2306 42-43 -0.6977830848590649 2306 44-45 -0.4301514056534188 2306 46-47 -0.4324115258960646 2306 48-49 -0.5591659047059281 2306 50-51 -0.6819137427591571 2306 52-53 -0.7168600396498022 2306 54-55 -0.39830781753343913 2306 56-57 -0.5618752276312122 2306 58-59 -0.12388998551503505 2306 60-61 -0.18062126222451624 2306 62-63 -0.12220428124724236 2306 64-65 -0.17364287122498467 2306 66-67 -0.3856792955357342 2306 68-69 -0.8433433480256305 2306 70-71 -0.519149364102045 2306 72-73 -1.1993749826499176 2306 74-75 -1.6319763554162634 2306 76-77 -1.3092334516930464 2306 78-79 -1.073527726709834 2306 80-81 -0.8019308746847926 2306 82-83 -0.6999364993112493 2306 84-85 -0.4277342776142241 2306 86-87 -0.39025046216267967 2306 88-89 -0.30328252323621996 2306 90-91 -0.2591537483913555 2306 92-93 -0.3035662985591401 2306 94-95 -0.3169856903634738 2306 96-97 -0.2685686477015743 2306 98-99 -0.3047017247563808 2306 100-101 -0.5044382960667093 2307 1 -1.0197148110399432 2307 2 -1.228470921507924 2307 3 -1.1700616299684157 2307 4 -1.01243322013503 2307 5 -1.260154115344509 2307 6 -1.9300647849306252 2307 7 -0.0990282128241482 2307 8 -1.8088827697870329 2307 9 -1.0760095241633891 2307 10-11 -1.0458432587141466 2307 12-13 -1.0473364555322924 2307 14-15 -0.53500532575449 2307 16-17 -0.8226797667084895 2307 18-19 -0.6037314160855161 2307 20-21 -0.7450136465113815 2307 22-23 -1.1382973072054625 2307 24-25 -0.4402512898080033 2307 26-27 -1.700416324636997 2307 28-29 -1.0802816648911637 2307 30-31 -0.8289980523824205 2307 32-33 -0.9147816665415078 2307 34-35 -1.1810375964311284 2307 36-37 -0.9319290183600053 2307 38-39 -0.9503657727180759 2307 40-41 -1.29270187036564 2307 42-43 -0.739569029948175 2307 44-45 -0.5059850761820961 2307 46-47 -0.6768637896672978 2307 48-49 -0.7324290914326568 2307 50-51 -0.8423107286501903 2307 52-53 -0.7609438482723725 2307 54-55 -0.572111684981568 2307 56-57 -0.774429888321869 2307 58-59 -0.35745972572840046 2307 60-61 -0.4138223189046819 2307 62-63 -0.3035655901647445 2307 64-65 -0.39984780051949187 2307 66-67 -0.6304270810325576 2307 68-69 -0.9004173939176887 2307 70-71 -0.6639044106523322 2307 72-73 -1.1944749134887758 2307 74-75 -1.371370255910854 2307 76-77 -1.1763382176689774 2307 78-79 -1.0395461146583074 2307 80-81 -0.9198154557511451 2307 82-83 -0.8895899391526356 2307 84-85 -0.7864681199983288 2307 86-87 -0.6376655822590855 2307 88-89 -0.4791719747211225 2307 90-91 -0.38043106062342247 2307 92-93 -0.39908537804027233 2307 94-95 -0.5409183509441888 2307 96-97 -0.4218602360152808 2307 98-99 -0.47783012522505786 2307 100-101 -0.590530134059847 2201 1 -0.8170852840565601 2201 2 -0.9669155217973824 2201 3 -0.8703399591810168 2201 4 -0.5665539129287502 2201 5 -0.7579850301363642 2201 6 -1.2182629910757399 2201 7 0.07480538043743934 2201 8 -1.3811145107915905 2201 9 -0.6605186358286126 2201 10-11 -0.6560511215932294 2201 12-13 -0.6220594779363644 2201 14-15 -0.25493827633869515 2201 16-17 -0.36560530504991107 2201 18-19 -0.33158703220085073 2201 20-21 -0.4947485719863529 2201 22-23 -0.8131177536780996 2201 24-25 -0.22915575892566054 2201 26-27 -1.1162916642811567 2201 28-29 -0.5669837948006382 2201 30-31 -0.38463178679662846 2201 32-33 -0.5743620678151586 2201 34-35 -0.7547611530987055 2201 36-37 -0.560547053948401 2201 38-39 -0.4845405640173084 2201 40-41 -0.8293981669820916 2201 42-43 -0.41770660906773927 2201 44-45 -0.28532071448009333 2201 46-47 -0.4376738198858341 2201 48-49 -0.43396651983254486 2201 50-51 -0.39323253122309865 2201 52-53 -0.3689516714219998 2201 54-55 -0.20761965867399468 2201 56-57 -0.23758535607852238 2201 58-59 0.11533082705936337 2201 60-61 -0.09687786008315058 2201 62-63 -0.08085077020893294 2201 64-65 -0.08386896895153839 2201 66-67 -0.2495121238309359 2201 68-69 -0.5594804124780204 2201 70-71 -0.22244583207010393 2201 72-73 -0.7612084778107757 2201 74-75 -0.900301274394586 2201 76-77 -0.7532204813588912 2201 78-79 -0.7298162511127586 2201 80-81 -0.5484520640523272 2201 82-83 -0.4371092822676985 2201 84-85 -0.2088529455305732 2201 86-87 -0.19454380378840597 2201 88-89 -0.1861453631383796 2201 90-91 -0.11496581647531201 2201 92-93 -0.18461238203405728 2201 94-95 -0.06567200871115375 2201 96-97 -0.08049408263481794 2201 98-99 -0.1498842757187866 2201 100-101 -0.14883226356941037 1101 1 -0.7424696424469275 1101 2 -0.8830895551767348 1101 3 -0.6771364925437418 1101 4 -0.541390759407193 1101 5 -0.7428696859627735 1101 6 -1.0226090834628003 1101 7 0.3561930366950712 1101 8 -1.1792378706823357 1101 9 -0.5745283275873128 1101 10-11 -0.44623983773453446 1101 12-13 -0.45214117289194533 1101 14-15 -0.20266238100327882 1101 16-17 -0.32117214404614103 1101 18-19 -0.3103579593852004 1101 20-21 -0.36191532363644896 1101 22-23 -0.6087568055820256 1101 24-25 -0.10798935617372507 1101 26-27 -0.9681816401965975 1101 28-29 -0.4710632640462826 1101 30-31 -0.17349811595665443 1101 32-33 -0.4319847237942014 1101 34-35 -0.7081561761286288 1101 36-37 -0.30361513479464364 1101 38-39 -0.4492082234964201 1101 40-41 -0.7615804606220244 1101 42-43 -0.231741348824972 1101 44-45 -0.09836587233075988 1101 46-47 -0.2966676070237675 1101 48-49 -0.2504536317360859 1101 50-51 -0.16942394370777691 1101 52-53 -0.21143645290324642 1101 54-55 -0.0479607692129882 1101 56-57 -0.09689463928019393 1101 58-59 0.2075025979650178 1101 60-61 0.03626562725484206 1101 62-63 0.08518083933525844 1101 64-65 0.02365914907188227 1101 66-67 -0.14695208534485005 1101 68-69 -0.3756398333328228 1101 70-71 -0.0985629159423489 1101 72-73 -0.5047994409907162 1101 74-75 -0.5709008535281299 1101 76-77 -0.486468845567952 1101 78-79 -0.40993428426266476 1101 80-81 -0.3785415552153246 1101 82-83 -0.3233040728705099 1101 84-85 -0.10943399186314551 1101 86-87 0.017770563327278666 1101 88-89 0.003432187560751032 1101 90-91 0.04191819534145225 1101 92-93 0.06840566218074073 1101 94-95 0.014351411382044432 1101 96-97 -0.008321336130364898 1101 98-99 0.02114026824420634 1101 100-101 -0.09537538585892946 2304 1 -0.8120486812274237 2304 2 -1.0581382991804809 2304 3 -0.7875253104239839 2304 4 -0.7478221309407438 2304 5 -0.9559577611621677 2304 6 -1.291579514776707 2304 7 -0.08300222599894624 2304 8 -1.3962005237727162 2304 9 -0.8343057359466499 2304 10-11 -0.6481386087465317 2304 12-13 -0.701090370790407 2304 14-15 -0.5889783091683789 2304 16-17 -0.7902317206712866 2304 18-19 -0.6452329303253919 2304 20-21 -0.6744274259406886 2304 22-23 -0.8347646677085194 2304 24-25 -0.4515336546849831 2304 26-27 -1.194256491413224 2304 28-29 -0.681663127707143 2304 30-31 -0.44837497947350613 2304 32-33 -0.636741717141053 2304 34-35 -0.7877686637581824 2304 36-37 -0.47626401289081244 2304 38-39 -0.4902423256081079 2304 40-41 -0.8667004961227533 2304 42-43 -0.47998387687582067 2304 44-45 -0.3610823278532749 2304 46-47 -0.4387450570743674 2304 48-49 -0.4149220026991358 2304 50-51 -0.456110466562734 2304 52-53 -0.47225976056240526 2304 54-55 -0.2893756778047738 2304 56-57 -0.24198586852016035 2304 58-59 -0.07080080035249381 2304 60-61 -0.1643939249229298 2304 62-63 -0.18796776277601168 2304 64-65 -0.19761108339454125 2304 66-67 -0.38143113203964063 2304 68-69 -0.5393070960151611 2304 70-71 -0.33855404456857485 2304 72-73 -0.6786359077696815 2304 74-75 -0.7235308464763435 2304 76-77 -0.6799324176242543 2304 78-79 -0.6114386098031126 2304 80-81 -0.5817848362984357 2304 82-83 -0.4864932727186755 2304 84-85 -0.44135214496726505 2304 86-87 -0.16116675297235972 2304 88-89 -0.18470211836151051 2304 90-91 -0.22012789327949633 2304 92-93 -0.1521748143106585 2304 94-95 -0.17360737741204701 2304 96-97 -0.26436236531380786 2304 98-99 -0.15772942429840242 2304 100-101 -0.20304065771817648 2202 1 -0.715933465179166 2202 2 -0.9329072000328864 2202 3 -0.7154938275965854 2202 4 -0.7118276691890344 2202 5 -0.9034735797651017 2202 6 -1.1348564189445867 2202 7 -0.006175033981829614 2202 8 -1.133324266621898 2202 9 -0.71684350114732 2202 10-11 -0.49596910107198156 2202 12-13 -0.5675843377178929 2202 14-15 -0.5298988742662409 2202 16-17 -0.6478965309325346 2202 18-19 -0.5396083004487835 2202 20-21 -0.6100157921591745 2202 22-23 -0.7682880705213861 2202 24-25 -0.4581821938477191 2202 26-27 -0.8232460171483815 2202 28-29 -0.4753616200402071 2202 30-31 -0.3386598968470018 2202 32-33 -0.6152494315375421 2202 34-35 -0.7132547555383404 2202 36-37 -0.41152643696956304 2202 38-39 -0.4567055206449737 2202 40-41 -0.7945339537604497 2202 42-43 -0.4669145024673149 2202 44-45 -0.36617514495238623 2202 46-47 -0.3886250230568997 2202 48-49 -0.3208577942890116 2202 50-51 -0.350276327168487 2202 52-53 -0.43994578647365046 2202 54-55 -0.16677411629279604 2202 56-57 -0.1969569792164414 2202 58-59 -0.11599188220185752 2202 60-61 -0.147733650805133 2202 62-63 -0.1518153471105137 2202 64-65 -0.2358172747842815 2202 66-67 -0.2828175741037988 2202 68-69 -0.38318542455941795 2202 70-71 -0.16197837930953796 2202 72-73 -0.48720343416352563 2202 74-75 -0.5379143813003964 2202 76-77 -0.3903040787429255 2202 78-79 -0.31783561644092373 2202 80-81 -0.38026632192564236 2202 82-83 -0.28779474607287625 2202 84-85 -0.2171634666816722 2202 86-87 -0.060468710856962815 2202 88-89 -0.17805213748725635 2202 90-91 -0.026010491481244458 2202 92-93 -0.176315898815659 2202 94-95 -0.06150787694202009 2202 96-97 -0.16309363611157934 2202 98-99 -0.06960244342281996 2202 100-101 -0.226303682240939 1102 1 -0.629461367541758 1102 2 -0.830809027630071 1102 3 -0.6470809819952166 1102 4 -0.446937159287085 1102 5 -0.7008665659788953 1102 6 -0.9075656983114015 1102 7 0.060365436141037065 1102 8 -0.8081360213792408 1102 9 -0.43648966573524106 1102 10-11 -0.24683014356969224 1102 12-13 -0.3373303923440609 1102 14-15 -0.44531988182913196 1102 16-17 -0.41503111174143825 1102 18-19 -0.24695844209664486 1102 20-21 -0.39101172546935814 1102 22-23 -0.5944765248410917 1102 24-25 -0.539972803137168 1102 26-27 -0.7459213018653514 1102 28-29 -0.44493740303477836 1102 30-31 -0.25593595277243963 1102 32-33 -0.5848687155392334 1102 34-35 -0.5169699404678774 1102 36-37 -0.37344012741178645 1102 38-39 -0.3723446875907399 1102 40-41 -0.6433032521630153 1102 42-43 -0.3302840946665775 1102 44-45 -0.21701032241084306 1102 46-47 -0.2917416320345616 1102 48-49 -0.20941313474171253 1102 50-51 -0.24206113318279066 1102 52-53 -0.3804343409974784 1102 54-55 -0.12326748178720592 1102 56-57 -0.12839860678018766 1102 58-59 0.045679820146975914 1102 60-61 -0.15139584930938454 1102 62-63 0.0032850072525647533 1102 64-65 0.014900570354857479 1102 66-67 -0.05377278398019314 1102 68-69 -0.370216732616381 1102 70-71 -0.1878488627297905 1102 72-73 -0.3560376637083422 1102 74-75 -0.16463278886410393 1102 76-77 -0.2798672187011064 1102 78-79 -0.38621961841422703 1102 80-81 -0.2939938002083764 1102 82-83 -0.2102768151252512 1102 84-85 -0.14374065039820394 1102 86-87 0.003920579498700505 1102 88-89 0.040268633599765735 1102 90-91 0.10012269404987251 1102 92-93 -0.018272866216495487 1102 94-95 0.10674273764711728 1102 96-97 0.042598101618075646 1102 98-99 0.01767677849649374 1102 100-101 0.08469603686086202 2305 1 -0.33163245781239326 2305 2 -0.6156550679586168 2305 3 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90-91 -0.3993102987113488 1213 92-93 -0.4153855054043838 1213 94-95 -0.5079804312227694 1213 96-97 -0.38901214177343135 1213 98-99 -0.3705755472421757 1213 100-101 -0.3049591008587029 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 498.0 3 670.0 4 1239.0 5 727.0 6 1658.0 7 22131.0 8 179708.0 9 350233.0 10 323293.0 11 214960.0 12 128151.0 13 81752.0 14 54606.0 15 39223.0 16 31146.0 17 26898.0 18 23760.0 19 21978.0 20 20343.0 21 19094.0 22 18355.0 23 18320.0 24 20575.0 25 24195.0 26 28877.0 27 34148.0 28 39585.0 29 46976.0 30 57867.0 31 74364.0 32 100501.0 33 143385.0 34 218554.0 35 370411.0 36 724416.0 37 1149676.0 38 378479.0 39 737.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.496862990350934 19.37030201194111 15.377950454916576 12.75488454279138 2 30.667381905775077 36.205909392209314 21.569355817207843 11.557352884807772 3 27.836855171727493 23.83425417024963 34.96675717873599 13.362133479286886 4 22.07131053628651 18.12870178167004 31.61287190348339 28.187115778560056 5 41.318919821194015 16.083028699359218 22.331587953367276 20.266463526079484 6 46.524273768740116 15.606532806751607 23.760285363487217 14.108908061021058 7 42.79834491410828 22.250348887180746 19.744457561902784 15.206848636808193 8 23.77392797210944 42.72018386243042 18.86456688082127 14.641321284638877 9 39.58409027291532 26.445724524521637 19.00864775901771 14.961537443545343 10-11 32.33945315072481 33.233544368316906 21.111711083381188 13.315291397577097 12-13 37.7658523909045 20.730897196490304 20.837900847627726 20.665349564977472 14-15 29.51680537463986 20.352995482633162 24.885591990015595 25.244607152711385 16-17 37.539117533507365 20.15133817429154 25.52716374660931 16.78238054559178 18-19 38.448873106514384 20.263301040328127 26.405431432317318 14.882394420840168 20-21 33.591984863452076 27.703846470782008 23.61579962399224 15.088369041773673 22-23 44.044899724330016 20.528294099852236 20.5428531807932 14.883952995024543 24-25 30.810255564118407 33.35763684179815 20.734900267415725 15.097207326667728 26-27 23.840418135126495 40.1759058010684 20.815208405613735 15.16846765819137 28-29 30.1639749031268 34.079770284302455 20.586159332370702 15.170095480200038 30-31 40.08365846041006 22.40761122126876 22.4055833077418 15.103147010579384 32-33 28.05464207704243 21.563939707773685 35.0734642924605 15.307953922723383 34-35 18.472519618803126 29.958759296829644 27.106299082149864 24.462422002217362 36-37 28.56763744355426 33.811447200884636 21.14033024065945 16.480585114901654 38-39 33.88271801759958 24.359182033868517 20.841154391734975 20.916945556796925 40-41 26.76528988630626 22.11762680831218 20.79199986181448 30.32508344356708 42-43 36.41936746636676 21.44907131585828 24.591820057785416 17.539741159989543 44-45 29.318748757829503 27.266690628819724 26.5600581840444 16.854502429306375 46-47 31.78635272837252 30.25198815499277 21.706424744216488 16.25523437241822 48-49 21.35262929176947 30.046526547710478 28.19808878298133 20.402755377538725 50-51 17.790857300469952 24.109711831867653 33.750418479660006 24.34901238800239 52-53 35.20971141672036 22.29778805217712 22.523648501035336 19.968852030067183 54-55 38.73488835058605 21.74484833671184 23.17196566474989 16.34829764795222 56-57 40.812715080878895 21.383088253830763 21.93257241125307 15.871624254037267 58-59 36.20716302932134 21.78200311705027 22.088485944903187 19.9223479087252 60-61 26.3999241755116 21.70688835155333 21.03113472772613 30.862052745208935 62-63 34.159295304947825 21.57496601327623 21.90429663871528 22.36144204306066 64-65 26.410734185348417 25.157458813135626 28.598080456192836 19.83372654532312 66-67 19.88933501609974 26.83462201512281 25.628301399929114 27.64774156884834 68-69 17.697203995574394 23.77286037328456 39.68185977374558 18.84807585739547 70-71 16.99879746610507 33.40901794002269 31.99280286776926 17.599381726102976 72-73 16.354334562865557 44.30321421085473 22.138576954984345 17.203874271295362 74-75 16.060785038344545 46.03646222836375 21.12201171635188 16.780741016939828 76-77 16.12865722879936 46.05465602022707 20.860632379075255 16.956054371898315 78-79 16.15246173406895 45.10479407439297 21.25071383420734 17.492030357330744 80-81 16.342296185166987 43.90545018314805 21.52686897660191 18.225384655083054 82-83 16.664460554309333 42.71102150969591 21.685589017939208 18.93892891805555 84-85 16.923278081104446 41.8531918124295 21.764322142561035 19.45920796390502 86-87 17.292002020129594 40.73470414129579 21.9906310414463 19.982662797128313 88-89 17.983752906969155 39.846842216573144 21.96157327711302 20.207831599344686 90-91 18.38320940963263 38.97233365894098 22.064189156622636 20.58026777480375 92-93 18.727967199280474 38.36688702269983 22.020507778284532 20.88463799973517 94-95 19.043897918038038 37.84100664050967 22.14137688955664 20.973718551895654 96-97 19.406517281595654 37.3368616687336 22.256179154472385 21.00044189519836 98-99 19.745718004293092 36.85353868731988 22.27864766632213 21.1220956420649 100-101 20.046607430156634 37.38312745556609 21.855534840695857 20.714730273581416 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 86.0 1 159.0 2 236.0 3 253.0 4 259.5 5 279.5 6 375.0 7 539.0 8 738.5 9 938.0 10 1131.0 11 1316.0 12 1451.0 13 1537.5 14 1567.5 15 1649.0 16 1673.0 17 1638.0 18 1711.0 19 1860.0 20 2166.0 21 2790.0 22 3714.5 23 4942.0 24 6635.0 25 8912.5 26 11806.0 27 15306.5 28 19468.0 29 24203.0 30 29801.5 31 36822.0 32 45636.5 33 56935.0 34 71365.0 35 88924.5 36 109984.5 37 133583.0 38 154145.0 39 167613.5 40 178345.5 41 187801.5 42 195634.0 43 204146.0 44 210770.0 45 217753.0 46 226464.0 47 233047.0 48 235257.5 49 230532.5 50 221438.5 51 213276.0 52 203882.5 53 191064.5 54 174731.5 55 154490.0 56 133348.5 57 111128.0 58 90125.0 59 73369.0 60 59582.0 61 48302.5 62 38734.5 63 30517.5 64 24131.0 65 19171.5 66 15207.5 67 12117.0 68 9519.0 69 7438.5 70 5887.5 71 4646.5 72 3618.0 73 2762.5 74 2107.5 75 1625.5 76 1235.5 77 951.5 78 738.0 79 569.0 80 429.0 81 336.0 82 249.5 83 186.0 84 152.5 85 112.5 86 88.5 87 75.0 88 68.5 89 49.5 90 37.5 91 34.0 92 24.5 93 20.0 94 16.0 95 14.5 96 10.0 97 5.5 98 3.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18104818021235747 2 0.1710912314942495 3 0.14534741036191803 4 0.13815516772650405 5 0.14208185172801144 6 0.12276897735325071 7 0.09856778207865428 8 0.08578602497170684 9 0.08616667291062848 10-11 0.06602238330085472 12-13 0.08566582035941579 14-15 0.09041390254491195 16-17 0.08245034698063043 18-19 0.07869395284653537 20-21 0.07665047443758766 22-23 0.09837745810919347 24-25 0.4347199803505527 26-27 0.27582951700384395 28-29 0.11715942877966876 30-31 0.2203851395846009 32-33 0.2505364631676039 34-35 0.23439899396753153 36-37 0.23675300095823112 38-39 0.2539622946178986 40-41 0.25393224346482585 42-43 0.27918522909696886 44-45 0.25652665968010746 46-47 0.2743770446053272 48-49 0.25911105884436486 50-51 0.2734354418090474 52-53 0.27319503258446526 54-55 0.24974511613668784 56-57 0.2659426876429058 58-59 0.25530457945514856 60-61 0.23174447544610435 62-63 0.22509315356600007 64-65 0.2231598627183191 66-67 0.2322753791503898 68-69 0.2369934101828132 70-71 0.22382098808591985 72-73 0.21502601728662527 74-75 0.2240714143615262 76-77 0.2250330512598545 78-79 0.22720675133211754 80-81 0.2331568796405241 82-83 0.2583798141195944 84-85 0.2529305383624005 86-87 0.25243970286221207 88-89 0.25054648021862813 90-91 0.2420319868480127 92-93 0.22014473036001883 94-95 0.21882247962481735 96-97 0.21376386885756937 98-99 0.23760445029529265 100-101 0.2452174090737253 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4991489.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.09445255840465 #Duplication Level Percentage of deduplicated Percentage of total 1 94.32796057429871 75.55146363149234 2 3.1524611771937003 5.049893043979067 3 0.7611109538320516 1.8288229555015012 4 0.5018129387752279 1.6076973047170442 5 0.2719706564644071 1.0891670420733313 6 0.1855843801910043 0.8918567600873595 7 0.13266735890834655 0.7438143638893385 8 0.10649621660090802 0.6823804934552811 9 0.06545654420579868 0.47184354670756135 >10 0.4372907060147998 6.621100346409908 >50 0.03778452265322128 2.1581964780230996 >100 0.01886170826245342 2.4184012707806892 >500 4.912405813346832E-4 0.22981345051056576 >1k 2.61191894222371E-5 0.039643441273921554 >5k 0.0 0.0 >10k+ 2.49028285834799E-5 0.6159058710990953 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT 30879 0.6186330371558466 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.006831628798540876 0.0 0.0 2 0.0072924131456565365 0.0 0.0 3 0.008113811329645322 0.0 0.0 4 0.008474425166518447 0.0 2.003410204850697E-5 5 0.009556266677137824 0.0 2.003410204850697E-5 6 0.01057800588161168 0.0 2.003410204850697E-5 7 0.011920290718861646 0.0 2.003410204850697E-5 8 0.013523018882742203 0.0 2.003410204850697E-5 9 0.014564792189264566 0.0 2.003410204850697E-5 10-11 0.023009166202710256 0.0 2.003410204850697E-5 12-13 0.029430095909256736 0.0 2.003410204850697E-5 14-15 0.03505967858488719 0.0 2.003410204850697E-5 16-17 0.03805477684113899 0.0 2.003410204850697E-5 18-19 0.04114002855660906 0.0 2.003410204850697E-5 20-21 0.044435638343588456 0.0 2.003410204850697E-5 22-23 0.04818201542665926 0.0 4.006820409701394E-5 24-25 0.052018545968948345 0.0 4.006820409701394E-5 26-27 0.056446082521668384 0.0 4.006820409701394E-5 28-29 0.06178517071759549 0.0 4.006820409701394E-5 30-31 0.10831437272525293 0.0 4.006820409701394E-5 32-33 0.11680883199381989 0.0 4.006820409701394E-5 34-35 0.12309954003705106 0.0 8.013640819402787E-5 36-37 0.12958057204974308 0.0 8.013640819402787E-5 38-39 0.1369130533994966 1.0017051024253484E-5 8.013640819402787E-5 40-41 0.14525725690269978 2.003410204850697E-5 8.013640819402787E-5 42-43 0.15455308025320702 2.003410204850697E-5 8.013640819402787E-5 44-45 0.1647203870428243 2.003410204850697E-5 8.013640819402787E-5 46-47 0.17540858048570276 2.003410204850697E-5 8.013640819402787E-5 48-49 0.18737895645968566 2.003410204850697E-5 1.0017051024253484E-4 50-51 0.20054136150555474 2.003410204850697E-5 1.0017051024253484E-4 52-53 0.21501600023560102 2.003410204850697E-5 1.0017051024253484E-4 54-55 0.2303721394557816 2.003410204850697E-5 1.0017051024253484E-4 56-57 0.2468101201865816 2.003410204850697E-5 1.0017051024253484E-4 58-59 0.2649009143363834 2.003410204850697E-5 1.0017051024253484E-4 60-61 0.28425385691524113 2.003410204850697E-5 1.0017051024253484E-4 62-63 0.3049090161272518 2.003410204850697E-5 1.202046122910418E-4 64-65 0.3270266647888035 3.0051153072760455E-5 1.202046122910418E-4 66-67 0.3521494287576312 4.006820409701394E-5 1.302216633152953E-4 68-69 0.3781637102676175 4.006820409701394E-5 1.4023871433954877E-4 70-71 0.40641179415601236 4.006820409701394E-5 1.4023871433954877E-4 72-73 0.4368035269635974 4.006820409701394E-5 1.4023871433954877E-4 74-75 0.4674356689957646 4.006820409701394E-5 1.4023871433954877E-4 76-77 0.5032866946115678 4.006820409701394E-5 1.5025576536380225E-4 78-79 0.5417521705447013 4.006820409701394E-5 1.6027281638805575E-4 80-81 0.5853563936532766 4.006820409701394E-5 1.6027281638805575E-4 82-83 0.6321059707834676 4.006820409701394E-5 1.6027281638805575E-4 84-85 0.6843949771300708 4.006820409701394E-5 1.6027281638805575E-4 86-87 0.738226609334409 4.006820409701394E-5 1.6027281638805575E-4 88-89 0.797267108071359 4.006820409701394E-5 1.6027281638805575E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 15670 0.0 89.14966 9 ATCGGGA 25600 0.0 87.4853 2 TCGGGAG 23310 0.0 85.05248 3 GAGAGCG 18875 0.0 84.84186 7 GGATCGG 7845 0.0 82.551636 1 GATCGGG 27925 0.0 81.577675 1 TCCGGAG 3770 0.0 80.44968 3 AGAGCGG 17890 0.0 80.20894 8 GAGCGTC 31255 0.0 80.17972 9 CGGGAGA 23865 0.0 80.14285 4 ATTGGGA 11125 0.0 77.70108 2 GGAGAGC 21105 0.0 77.10875 6 AGAGCGT 33080 0.0 76.74646 8 CGGAAGA 33545 0.0 75.67737 4 CCGGAGA 4685 0.0 75.570175 4 CGGAGAG 9740 0.0 75.37787 5 AAGAGCG 32545 0.0 75.09072 7 GAGGGGC 20195 0.0 74.623695 9 CGGAAAG 9110 0.0 74.59971 5 TCCGGAA 2545 0.0 74.599686 3 >>END_MODULE