FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_30749-1.3420000003587d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_30749-1.3420000003587d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4991489
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT308790.6186330371558466Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC156700.089.149669
ATCGGGA256000.087.48532
TCGGGAG233100.085.052483
GAGAGCG188750.084.841867
GGATCGG78450.082.5516361
GATCGGG279250.081.5776751
TCCGGAG37700.080.449683
AGAGCGG178900.080.208948
GAGCGTC312550.080.179729
CGGGAGA238650.080.142854
ATTGGGA111250.077.701082
GGAGAGC211050.077.108756
AGAGCGT330800.076.746468
CGGAAGA335450.075.677374
CCGGAGA46850.075.5701754
CGGAGAG97400.075.377875
AAGAGCG325450.075.090727
GAGGGGC201950.074.6236959
CGGAAAG91100.074.599715
TCCGGAA25450.074.5996863
Produced by FastQC (version 0.11.2)