##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_30521-1.342000000356dc.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3128253 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.846453915332297 33.0 33.0 33.0 27.0 33.0 2 31.70228207245386 33.0 33.0 33.0 27.0 33.0 3 31.650012962506548 33.0 33.0 33.0 27.0 33.0 4 35.324853200812086 37.0 37.0 37.0 33.0 37.0 5 35.34821831865901 37.0 37.0 37.0 33.0 37.0 6 35.45459686284965 37.0 37.0 37.0 33.0 37.0 7 35.27584022136317 37.0 37.0 37.0 33.0 37.0 8 35.2975846263074 37.0 37.0 37.0 33.0 37.0 9 35.12251470709051 37.0 37.0 37.0 33.0 37.0 10-11 35.06740167754974 37.0 37.0 37.0 33.0 37.0 12-13 35.07777679746491 37.0 37.0 37.0 33.0 37.0 14-15 36.811055883267755 40.0 37.0 40.0 33.0 40.0 16-17 36.7448221099764 40.0 37.0 40.0 33.0 40.0 18-19 36.69981823720779 40.0 37.0 40.0 33.0 40.0 20-21 36.59717484487348 40.0 37.0 40.0 33.0 40.0 22-23 36.812943358481554 40.0 37.0 40.0 33.0 40.0 24-25 36.52350720993475 40.0 37.0 40.0 33.0 40.0 26-27 36.454753180129615 40.0 37.0 40.0 33.0 40.0 28-29 36.380553299237626 40.0 37.0 40.0 33.0 40.0 30-31 36.331533766610306 40.0 37.0 40.0 33.0 40.0 32-33 36.23963710735673 40.0 37.0 40.0 33.0 40.0 34-35 36.190829194441754 40.0 37.0 40.0 33.0 40.0 36-37 36.08806656622722 40.0 37.0 40.0 33.0 40.0 38-39 35.99571278282159 38.5 37.0 40.0 30.0 40.0 40-41 35.92515726829 37.0 37.0 40.0 27.0 40.0 42-43 35.82907041086511 37.0 37.0 40.0 27.0 40.0 44-45 35.644341106681594 37.0 37.0 40.0 27.0 40.0 46-47 35.51886644078979 37.0 37.0 40.0 27.0 40.0 48-49 35.38402792229401 37.0 37.0 40.0 27.0 40.0 50-51 34.17977430214244 37.0 35.0 38.5 24.5 38.5 52-53 34.598205292219006 37.0 35.0 38.5 27.0 40.0 54-55 35.31949477871515 37.0 37.0 40.0 27.0 40.0 56-57 35.36215421195153 37.0 37.0 40.0 27.0 40.0 58-59 35.07249733317606 37.0 37.0 40.0 27.0 40.0 60-61 34.80634974217239 37.0 33.0 40.0 27.0 40.0 62-63 34.52737278602466 37.0 33.0 38.5 27.0 40.0 64-65 34.29271817209158 37.0 33.0 37.0 27.0 40.0 66-67 34.08624893830518 37.0 33.0 37.0 27.0 40.0 68-69 33.94881376282545 37.0 33.0 37.0 27.0 40.0 70-71 33.586614158125954 37.0 33.0 37.0 27.0 38.5 72-73 33.45429030196726 37.0 33.0 37.0 27.0 37.0 74-75 33.31410183255638 37.0 33.0 37.0 27.0 37.0 76-77 33.085305600282325 37.0 33.0 37.0 27.0 37.0 78-79 32.868090912084156 37.0 33.0 37.0 22.0 37.0 80-81 32.63057064118535 37.0 33.0 37.0 22.0 37.0 82-83 32.41024718908605 37.0 33.0 37.0 22.0 37.0 84-85 32.26217524605587 37.0 33.0 37.0 22.0 37.0 86-87 32.13799794965433 33.0 33.0 37.0 22.0 37.0 88-89 32.00619083558779 33.0 33.0 37.0 22.0 37.0 90-91 31.86068038614524 33.0 33.0 37.0 22.0 37.0 92-93 31.732036379410488 33.0 33.0 37.0 22.0 37.0 94-95 31.61539699634269 33.0 33.0 37.0 18.5 37.0 96-97 31.460255612317802 33.0 33.0 37.0 15.0 37.0 98-99 31.304670689998538 33.0 33.0 37.0 15.0 37.0 100-101 29.9327805327766 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14282995779716146 2306 2 0.08251447213961782 2306 3 0.11379254345945711 2306 4 0.1969333889243643 2306 5 0.13177138550797451 2306 6 0.10589451880821343 2306 7 0.2184645776034415 2306 8 -0.11449682699625185 2306 9 0.13758311282698799 2306 10-11 0.12143886381373648 2306 12-13 0.1142119889631843 2306 14-15 0.35555031182136076 2306 16-17 0.20409626417217197 2306 18-19 0.30231666719028283 2306 20-21 0.3218662758065989 2306 22-23 0.3002595860656072 2306 24-25 0.2793156618429933 2306 26-27 0.1523371397550619 2306 28-29 0.20045026694570112 2306 30-31 0.222758028309471 2306 32-33 0.12421268476740721 2306 34-35 0.1799995179912841 2306 36-37 0.17141104429119736 2306 38-39 0.10557009496167069 2306 40-41 0.11904950364723987 2306 42-43 0.1012719131169817 2306 44-45 0.041989270508501875 2306 46-47 0.0582361233443649 2306 48-49 -0.07149965368872557 2306 50-51 -0.04265501636020019 2306 52-53 0.017538579574100766 2306 54-55 0.0830413910047696 2306 56-57 0.0694471802352652 2306 58-59 0.043500686896230434 2306 60-61 0.2161543228524465 2306 62-63 0.21789048850716597 2306 64-65 0.0427470962225982 2306 66-67 -0.009486714320040335 2306 68-69 -0.015525869347968069 2306 70-71 0.02306675182355633 2306 72-73 -0.012753040271526572 2306 74-75 0.0296010860520326 2306 76-77 -0.03717920860310642 2306 78-79 0.16373943448731154 2306 80-81 0.08898990587534428 2306 82-83 0.026021828694489102 2306 84-85 0.08096745808988004 2306 86-87 0.10469831066301793 2306 88-89 0.07179972554758507 2306 90-91 0.0078455033206275 2306 92-93 -0.06327328327989434 2306 94-95 -0.025100856848112585 2306 96-97 -0.010235082093164038 2306 98-99 -0.16235824307366542 2306 100-101 -0.2989268308768196 2307 1 0.20424868845918098 2307 2 0.15668935713630816 2307 3 0.16660776714530812 2307 4 0.2381933040654829 2307 5 0.18473454913021214 2307 6 0.1099272148669499 2307 7 0.30620228942577654 2307 8 0.12992793654974832 2307 9 0.3053380043410385 2307 10-11 0.3165393196953161 2307 12-13 0.32009090212444136 2307 14-15 0.5226839555620018 2307 16-17 0.45207141959240715 2307 18-19 0.5222791957370205 2307 20-21 0.5974045739242229 2307 22-23 0.3422223825035857 2307 24-25 0.3411597198221159 2307 26-27 0.2239748881625374 2307 28-29 0.2707192377435561 2307 30-31 0.2945375524893237 2307 32-33 0.2570750759699081 2307 34-35 0.14862142416404112 2307 36-37 0.086813724136519 2307 38-39 0.039749651395091234 2307 40-41 0.06927204662812159 2307 42-43 0.12199300035555893 2307 44-45 0.1844227758386694 2307 46-47 0.1536462201783806 2307 48-49 -0.023162445262016718 2307 50-51 -0.03263725023991526 2307 52-53 -0.13111673303564686 2307 54-55 0.04063694054498512 2307 56-57 0.04011825444580808 2307 58-59 0.11457616358602252 2307 60-61 0.0971941206945246 2307 62-63 0.09055468684263701 2307 64-65 0.09451707761015626 2307 66-67 -0.0398667133737618 2307 68-69 0.015674796515021683 2307 70-71 -0.1853812849401919 2307 72-73 -0.029299988877063754 2307 74-75 -0.023925015683992967 2307 76-77 0.057673114686963345 2307 78-79 0.022949595162074843 2307 80-81 0.08970894727786316 2307 82-83 -0.057219375376654114 2307 84-85 -0.042688337657054376 2307 86-87 -0.03884424439296197 2307 88-89 0.08747019666964206 2307 90-91 0.129289863577025 2307 92-93 0.07760935170363936 2307 94-95 -0.049091450000627646 2307 96-97 -0.09019228613498953 2307 98-99 0.12158464278134318 2307 100-101 -0.23536980169733113 2201 1 0.11000707605341375 2201 2 0.05272110781388051 2201 3 0.10393016309348013 2201 4 0.06656299236691865 2201 5 0.09847320832490425 2201 6 0.12464806633873593 2201 7 0.2051641614253299 2201 8 0.09213197315772703 2201 9 0.08064095156828444 2201 10-11 0.0908986767987301 2201 12-13 0.09145574678248636 2201 14-15 0.24750848975076423 2201 16-17 0.25848714949831475 2201 18-19 0.30686487137877094 2201 20-21 0.4601153816816037 2201 22-23 0.3231081376911362 2201 24-25 0.24391302572092854 2201 26-27 0.25923000384216976 2201 28-29 0.22556187299255015 2201 30-31 0.21221756633016042 2201 32-33 0.10918834060637295 2201 34-35 0.13909121269448832 2201 36-37 0.04849905447273528 2201 38-39 0.06817003957601031 2201 40-41 0.09923587052424665 2201 42-43 0.11271141016798936 2201 44-45 0.14685770990931246 2201 46-47 0.1915564940748098 2201 48-49 0.07944887495764164 2201 50-51 0.024177021694853806 2201 52-53 0.16882541251560923 2201 54-55 0.23627359639525025 2201 56-57 0.2770953123438318 2201 58-59 0.2540875000010345 2201 60-61 0.21240106010637305 2201 62-63 0.29169434468511923 2201 64-65 0.16142807281724458 2201 66-67 0.13128960811095425 2201 68-69 0.17495859811491243 2201 70-71 0.1783945780146894 2201 72-73 0.17199056533538482 2201 74-75 0.3272622200440978 2201 76-77 0.22058509592378073 2201 78-79 0.21530604360787464 2201 80-81 0.1969546397393529 2201 82-83 0.12613142160983415 2201 84-85 0.18036901792309123 2201 86-87 0.26707700721437533 2201 88-89 0.35080354951544024 2201 90-91 0.2633064907960083 2201 92-93 0.22887727146059333 2201 94-95 0.26451413420572933 2201 96-97 0.31440299614203226 2201 98-99 0.33099449488338806 2201 100-101 0.19206514212954318 1101 1 0.2372442807031021 1101 2 0.25810138459803866 1101 3 0.242289196537115 1101 4 0.3662606643968971 1101 5 0.39631002267230997 1101 6 0.2705059052073082 1101 7 0.41895826580663 1101 8 0.21710104817704234 1101 9 0.320171338431976 1101 10-11 0.3602501230160513 1101 12-13 0.3416267831377624 1101 14-15 0.5058394785909783 1101 16-17 0.48616266271901765 1101 18-19 0.5758222194667795 1101 20-21 0.55920035950939 1101 22-23 0.38004605104760714 1101 24-25 0.46224361264803093 1101 26-27 0.4902630544966158 1101 28-29 0.38200833593393213 1101 30-31 0.4591518624686728 1101 32-33 0.3409470677029063 1101 34-35 0.27137644596039223 1101 36-37 0.26264773552789933 1101 38-39 0.29598778537936 1101 40-41 0.1784355275583991 1101 42-43 0.2914274317641201 1101 44-45 0.19386725311273523 1101 46-47 0.2280307783345492 1101 48-49 0.33171320874200916 1101 50-51 0.33716462044267814 1101 52-53 0.35836897319779126 1101 54-55 0.46840163697953585 1101 56-57 0.39727559270054513 1101 58-59 0.49159090509817815 1101 60-61 0.5237028566579625 1101 62-63 0.4787049596568238 1101 64-65 0.44728385746326893 1101 66-67 0.38067177835241495 1101 68-69 0.5029091985284353 1101 70-71 0.5303581468244474 1101 72-73 0.3438486096262139 1101 74-75 0.3790815467056774 1101 76-77 0.3905494596283461 1101 78-79 0.5130428262488067 1101 80-81 0.40136139366582313 1101 82-83 0.3811318905084633 1101 84-85 0.4396996539729088 1101 86-87 0.4249123032233797 1101 88-89 0.3931772483217557 1101 90-91 0.30744950041065877 1101 92-93 0.47629497975205837 1101 94-95 0.3328498566891902 1101 96-97 0.21585993115268565 1101 98-99 0.34639133941893263 1101 100-101 0.27962838762253384 2304 1 0.24328245574965734 2304 2 0.2344168940420417 2304 3 0.2700312821981967 2304 4 0.4115592810502591 2304 5 0.335380254116842 2304 6 0.2654174508311442 2304 7 0.3985991277379952 2304 8 0.27382890132947324 2304 9 0.31321403595791253 2304 10-11 0.3855321129069864 2304 12-13 0.2832528805040724 2304 14-15 0.48234918862024045 2304 16-17 0.3789223727482849 2304 18-19 0.4247744008980163 2304 20-21 0.5716158955562136 2304 22-23 0.42161561742164366 2304 24-25 0.3743947831721144 2304 26-27 0.38857909599701657 2304 28-29 0.3946280486234812 2304 30-31 0.3547088590103016 2304 32-33 0.2526975332522525 2304 34-35 0.19703985378162514 2304 36-37 0.3305048783850353 2304 38-39 0.2528330234746008 2304 40-41 0.28359481194254244 2304 42-43 0.2749060830011487 2304 44-45 0.38994366971289907 2304 46-47 0.33954082089906024 2304 48-49 0.2626968480077707 2304 50-51 0.30150287529768605 2304 52-53 0.32250903704456135 2304 54-55 0.4827648319782085 2304 56-57 0.4703980986861822 2304 58-59 0.43764936479490757 2304 60-61 0.39875040919853433 2304 62-63 0.4025351006517752 2304 64-65 0.2939462011717069 2304 66-67 0.15963593105260543 2304 68-69 0.2908907095686075 2304 70-71 0.43952281827962025 2304 72-73 0.48290055163206347 2304 74-75 0.44129656399750417 2304 76-77 0.356651813977912 2304 78-79 0.4270432252911007 2304 80-81 0.42927406990950345 2304 82-83 0.3329132818359426 2304 84-85 0.34934583646825246 2304 86-87 0.45225900447371004 2304 88-89 0.5334615659594206 2304 90-91 0.2740210619961907 2304 92-93 0.3375000874934777 2304 94-95 0.40487998438272754 2304 96-97 0.3588394469813636 2304 98-99 0.4244390249736014 2304 100-101 0.3219860625360731 2202 1 0.20538822164058956 2202 2 0.2790162092804671 2202 3 0.24832843398701243 2202 4 0.27424262844270686 2202 5 0.38134090513654684 2202 6 0.3305537828908882 2202 7 0.3869934114674649 2202 8 0.273656222323865 2202 9 0.32680345192576254 2202 10-11 0.36404424509574085 2202 12-13 0.3251470153723375 2202 14-15 0.48540084346070245 2202 16-17 0.4262658823994556 2202 18-19 0.4413486702375664 2202 20-21 0.4634488732766471 2202 22-23 0.34165134411245646 2202 24-25 0.3601173182785473 2202 26-27 0.4764275552834718 2202 28-29 0.3556828543595927 2202 30-31 0.3317300841380728 2202 32-33 0.34982500806989236 2202 34-35 0.23817609900565628 2202 36-37 0.39668733261021316 2202 38-39 0.24903861321310927 2202 40-41 0.10673497717848335 2202 42-43 0.2924184260766225 2202 44-45 0.26638098673765853 2202 46-47 0.3289437013635066 2202 48-49 0.32209879808964814 2202 50-51 0.24249267830468568 2202 52-53 0.451923144125395 2202 54-55 0.6058016627230103 2202 56-57 0.5059475206084798 2202 58-59 0.3999707933663359 2202 60-61 0.5455411475501393 2202 62-63 0.4979629525844871 2202 64-65 0.4255084375633089 2202 66-67 0.657334350642742 2202 68-69 0.6046128601169158 2202 70-71 0.5101797924273868 2202 72-73 0.38424160507323535 2202 74-75 0.3056659506893169 2202 76-77 0.32164207597364225 2202 78-79 0.322304848870715 2202 80-81 0.38732169309215436 2202 82-83 0.5636172092909177 2202 84-85 0.3859696641862129 2202 86-87 0.47368143537730134 2202 88-89 0.4020765984607877 2202 90-91 0.4058129788323903 2202 92-93 0.4521187546894332 2202 94-95 0.4930391294231704 2202 96-97 0.30748133652634735 2202 98-99 0.24693350470366582 2202 100-101 0.09784784490098275 1102 1 0.12584221670583418 1102 2 0.16131311866734777 1102 3 0.2406454920255534 1102 4 0.1960124208719236 1102 5 0.2727194686871357 1102 6 0.16374746411135988 1102 7 0.22837905564541217 1102 8 0.20816583929190813 1102 9 0.24184664836542424 1102 10-11 0.27377627909935853 1102 12-13 0.23659143106374358 1102 14-15 0.24356622523567495 1102 16-17 0.3218973774912186 1102 18-19 0.4022025454626146 1102 20-21 0.46198679291914146 1102 22-23 0.21961125392325442 1102 24-25 0.13772029066594627 1102 26-27 0.10485589356863301 1102 28-29 0.07449666937855426 1102 30-31 0.16257003336052378 1102 32-33 0.24195384135317966 1102 34-35 0.1698403185890598 1102 36-37 0.1556424234429059 1102 38-39 0.21149243345373492 1102 40-41 0.21211051105087364 1102 42-43 0.07750212739500029 1102 44-45 0.2462116928584166 1102 46-47 0.2555773889127195 1102 48-49 0.09994642114379815 1102 50-51 0.17083459603379225 1102 52-53 0.22012381602387876 1102 54-55 0.29201426282624254 1102 56-57 0.31150841930845985 1102 58-59 0.31514343612862206 1102 60-61 0.5429433991435104 1102 62-63 0.39434927253566343 1102 64-65 0.4284304072047149 1102 66-67 0.4486349350366723 1102 68-69 0.27136832292470814 1102 70-71 0.4188958025330791 1102 72-73 0.2653581023814482 1102 74-75 0.3311805992849344 1102 76-77 0.34173837250570216 1102 78-79 0.24032865500083744 1102 80-81 0.31382847135135705 1102 82-83 0.3309703393202952 1102 84-85 0.4070549998148678 1102 86-87 0.4079284694708676 1102 88-89 0.49988468301501143 1102 90-91 0.5526005535537699 1102 92-93 0.3323530565695556 1102 94-95 0.4911204985166684 1102 96-97 0.4944370565431164 1102 98-99 0.22824641446326055 1102 100-101 0.29731759355485465 2305 1 -0.028823187084583424 2305 2 0.003500849522275473 2305 3 0.0735957259301614 2305 4 0.11319705414603476 2305 5 0.11903092053923103 2305 6 0.0877731738192793 2305 7 0.11365019721996816 2305 8 -0.010478340002457287 2305 9 0.059958299229109 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-0.7708658156453296 1213 80-81 -0.7871363129398787 1213 82-83 -0.7238535971655438 1213 84-85 -0.8002739009695006 1213 86-87 -0.7577866690695814 1213 88-89 -0.8078459907889446 1213 90-91 -0.6551209220948699 1213 92-93 -0.8390756093312 1213 94-95 -0.9591561825965123 1213 96-97 -0.853500978550553 1213 98-99 -0.6437289404923483 1213 100-101 -0.5713776120419496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 319.0 3 140.0 4 149.0 5 188.0 6 597.0 7 2365.0 8 8958.0 9 23388.0 10 29573.0 11 22382.0 12 13923.0 13 9816.0 14 7793.0 15 7166.0 16 6934.0 17 7621.0 18 8142.0 19 9225.0 20 10043.0 21 11361.0 22 12535.0 23 13975.0 24 15972.0 25 18591.0 26 22045.0 27 26816.0 28 32534.0 29 40201.0 30 49796.0 31 65825.0 32 89850.0 33 129195.0 34 198707.0 35 340167.0 36 665484.0 37 985191.0 38 241211.0 39 75.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.498092990338456 25.05576922461083 19.025839900854713 16.420297884195996 2 33.85413197735482 23.051079744857443 28.609076004815943 14.485712272971796 3 39.763341682288946 19.63976561459582 23.016450771894238 17.580441931220996 4 33.46227224420287 18.473443962150604 29.118490633682892 18.945793159963635 5 35.873296359431144 16.847550679055328 28.0481461029347 19.231006858578827 6 31.972422463382888 17.438205469354862 31.51064179127885 19.078730275983403 7 36.35144237153366 17.95359388973949 24.56903240876286 21.125931329963986 8 31.643461685386143 25.60367184515594 22.471143343628885 20.281723125829036 9 31.262211463923173 23.71113967051323 24.065658044137393 20.960990821426208 10-11 25.30442510967173 28.483205899442517 28.54615648754911 17.666212503336652 12-13 26.087906589250952 25.951086434888772 28.107287604826663 19.853719371033606 14-15 25.493635517665197 26.04274599069368 28.20807295578529 20.255545535855834 16-17 25.608605732324012 26.13253125949278 28.716663192812046 19.54219981537116 18-19 25.14559211940665 26.122508522465527 28.619312597893188 20.112586760234635 20-21 24.258263518951935 27.183877452567383 28.162169200117216 20.39568982836347 22-23 25.125230342236005 26.546816709495364 27.74317244654092 20.584780501727707 24-25 24.027883087020744 26.919434536055416 28.275910341436333 20.776772035487507 26-27 23.10497445657618 27.49385956125413 28.744914354402486 20.656251627767205 28-29 23.377286767593457 27.916911910378907 27.818574382697975 20.88722693932967 30-31 23.655586002173894 27.914896139295813 27.881078232145274 20.548439626385015 32-33 23.444862524675788 27.634125794877246 28.281116570671667 20.639895109775306 34-35 22.774067253436165 27.507359719909264 28.106391412447774 21.6121816142068 36-37 21.331943655562863 27.984387755347285 28.84238826966692 21.841280319422935 38-39 21.32930415797564 28.156894255139942 28.953653693051066 21.560147893833356 40-41 22.0619159046993 27.452395001062463 29.239403314570062 21.246285779668174 42-43 22.977605499787128 27.185382502449308 28.65814132124153 21.17887067652203 44-45 22.122473312830834 27.686177160174296 28.708583003867055 21.48276652312781 46-47 22.58833482320533 28.153195066159455 28.357898188927948 20.900571921707268 48-49 21.616666490378343 28.259434870920085 28.744500204975242 21.37939843372633 50-51 21.510613910500364 27.81082255502264 27.966845123958116 22.711718410518877 52-53 23.13259798154397 27.06330419751915 27.566924428754884 22.237173392181994 54-55 23.898426519329224 26.96967118450848 27.94520644532399 21.18669585083831 56-57 24.410588641253018 27.104884874402245 26.998849088606715 21.48567739573802 58-59 23.65771005421788 27.55411531523678 27.004372430291756 21.78380220025359 60-61 22.47594343703637 27.32050103019447 27.06847989438504 23.135075638384116 62-63 23.22504614342535 27.16110716024525 27.47836373595371 22.13548296037569 64-65 22.635248522007796 27.82426955873544 28.20528019252548 21.335201726731285 66-67 22.489732831722904 27.726505356006744 27.772824372101756 22.0109374401686 68-69 22.691215384233306 26.95483797574022 29.115145416863275 21.238801223163193 70-71 22.240373313314265 28.252790069414708 28.36336273553764 21.143473881733385 72-73 22.18498686358974 29.55816455810769 27.020204649589203 21.23664392871337 74-75 22.539493243930607 29.08601498522032 26.88629332473782 21.488198446111248 76-77 22.155820241402164 28.668465570609435 26.769988027777863 22.405726160210538 78-79 21.899178078504775 28.750222090067396 27.17019044233292 22.18040938909491 80-81 21.40915729858218 28.861727265211478 27.61296977410852 22.11614566209782 82-83 20.897389072396884 28.813716052067583 27.790352050614338 22.498542824921195 84-85 20.89923043615242 29.384832884039152 27.479272159454936 22.236664520353493 86-87 20.73495881907636 29.283902550339185 27.780361356275087 22.20077727430937 88-89 21.114217430307328 29.105082010138574 27.732958181992416 22.047742377561686 90-91 21.615314863914932 28.944778097944845 26.916060484816224 22.523846553324 92-93 21.61835228342231 28.930413991693733 26.885582580068967 22.565651144814993 94-95 21.92644078244616 29.235873799254513 26.7535481704711 22.084137247828227 96-97 21.944111830802488 29.871889239256756 26.57224621935093 21.611752710589823 98-99 22.17964812546416 29.434006099842257 26.797070088901958 21.589275685791627 100-101 22.316086607200944 29.515395913459457 26.486768593560768 21.68174888577883 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 54.0 1 88.0 2 134.0 3 148.0 4 119.0 5 108.5 6 171.0 7 282.5 8 423.5 9 564.5 10 711.5 11 854.0 12 964.0 13 1028.5 14 1006.5 15 875.5 16 729.0 17 654.0 18 614.0 19 631.0 20 700.5 21 796.0 22 994.5 23 1274.0 24 1644.0 25 2147.0 26 2783.5 27 3590.5 28 4558.0 29 5745.0 30 7374.5 31 9715.0 32 14332.5 33 22687.0 34 36182.5 35 55607.0 36 84249.0 37 117295.5 38 138466.5 39 142284.0 40 143900.0 41 147745.0 42 157276.5 43 172896.5 44 173290.5 45 165102.5 46 164109.0 47 161990.0 48 153648.5 49 138654.0 50 124676.0 51 121017.0 52 117099.0 53 106260.0 54 91626.5 55 75697.5 56 61524.5 57 47459.5 58 34212.5 59 24937.0 60 18740.0 61 14832.5 62 11768.5 63 9119.5 64 7065.5 65 5136.5 66 3654.0 67 2796.5 68 2260.0 69 1646.5 70 1153.5 71 949.0 72 743.0 73 562.0 74 420.5 75 320.5 76 267.5 77 220.0 78 179.0 79 139.5 80 102.0 81 82.5 82 69.5 83 60.0 84 57.5 85 47.0 86 39.0 87 36.0 88 24.5 89 20.0 90 17.0 91 11.5 92 9.5 93 9.0 94 6.0 95 2.0 96 1.5 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17783088516178197 2 0.1691998697036333 3 0.14413795815108305 4 0.1372331457845641 5 0.14154865351363846 6 0.12230468571435879 7 0.09647557278775087 8 0.08410445063107108 9 0.08324134908525621 10-11 0.06366173068482632 12-13 0.0836569164962041 14-15 0.08770070707196637 16-17 0.08063606108585207 18-19 0.0766242372340089 20-21 0.07496196759021728 22-23 0.09628377244423646 24-25 0.439494503801323 26-27 0.2738109737287873 28-29 0.12179321813165368 30-31 0.22777889128532763 32-33 0.2569645102234378 34-35 0.2413487655889725 36-37 0.24376225324486225 38-39 0.26139190148622893 40-41 0.260528799940414 42-43 0.2878603488912182 44-45 0.26557954231962694 46-47 0.28314525711315547 48-49 0.26736967885909485 50-51 0.28234608901517877 52-53 0.2825538727206527 54-55 0.25792351194100993 56-57 0.27336343959392034 58-59 0.2636935056084019 60-61 0.23920699508639484 62-63 0.23263783332102614 64-65 0.2305280295423676 66-67 0.23989427965065485 68-69 0.24472125496243433 70-71 0.23107146384899174 72-73 0.22269618218219564 74-75 0.23158293143169684 76-77 0.2326698000449452 78-79 0.23445993658441308 80-81 0.24138073231289156 82-83 0.2668102611905111 84-85 0.26129600131447167 86-87 0.2595378314989229 88-89 0.25861079650526986 90-91 0.2504113318200286 92-93 0.22745922404613694 94-95 0.2259088379360621 96-97 0.22154538012110914 98-99 0.24552042306041102 100-101 0.2529207196476756 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3128253.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.644742493156706 #Duplication Level Percentage of deduplicated Percentage of total 1 85.85992670964552 43.483538786913044 2 7.091703894334576 7.183150351325825 3 2.3054725248478687 3.502801870379044 4 1.0423655636680709 2.1116134222281433 5 0.6494755737077027 1.6446261593010911 6 0.4955191975780607 1.5057265297053912 7 0.3090177128351812 1.0955085744653397 8 0.22126794151745333 0.8964846336113823 9 0.2487824635764684 1.133957142417904 >10 1.451482835469703 14.530078102847222 >50 0.1803810052625435 6.441459583917157 >100 0.1367908397706645 13.11478192583338 >500 0.0060945184437298985 2.079744969072709 >1k 0.001719219342554932 1.276527947982469 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 4779 0.15276897360923172 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 4278 0.13675364492577807 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0013426024046009066 0.0 0.0 2 0.0014704693002771834 0.0 0.0 3 0.0016303029198725294 0.0 0.0 4 0.0017581698155488064 0.0 0.0 5 0.0019499701590632215 0.0 0.0 6 0.0023016041221729827 0.0 0.0 7 0.002781104980959021 0.0 0.0 8 0.003100772220149713 0.0 0.0 9 0.0035483063550166816 0.0 0.0 10-11 0.005018775655293866 0.0 0.0 12-13 0.0067609621088831375 3.1966723919069206E-5 0.0 14-15 0.00864699882010822 3.1966723919069206E-5 0.0 16-17 0.010628935703090512 3.1966723919069206E-5 0.0 18-19 0.012498989052356059 3.1966723919069206E-5 0.0 20-21 0.015040343603922061 3.1966723919069206E-5 0.0 22-23 0.017597681517447596 3.1966723919069206E-5 0.0 24-25 0.020890254081111725 3.1966723919069206E-5 0.0 26-27 0.025205761810186068 3.1966723919069206E-5 0.0 28-29 0.030144620655682262 3.1966723919069206E-5 0.0 30-31 0.06279862913901145 3.1966723919069206E-5 0.0 32-33 0.07026285917411412 3.1966723919069206E-5 0.0 34-35 0.07636850344265633 3.1966723919069206E-5 0.0 36-37 0.0835130662385683 3.1966723919069206E-5 0.0 38-39 0.09227194859239327 6.393344783813841E-5 0.0 40-41 0.10242138343669774 6.393344783813841E-5 0.0 42-43 0.11442488826830821 6.393344783813841E-5 0.0 44-45 0.12906564782324192 6.393344783813841E-5 0.0 46-47 0.14477729262946443 6.393344783813841E-5 0.0 48-49 0.16061680433136322 6.393344783813841E-5 0.0 50-51 0.17987675549260243 6.393344783813841E-5 0.0 52-53 0.20006374164749463 6.393344783813841E-5 0.0 54-55 0.22459820225538024 6.393344783813841E-5 0.0 56-57 0.2507150156972598 6.393344783813841E-5 0.0 58-59 0.27938916705266487 6.393344783813841E-5 0.0 60-61 0.3103329558063238 6.393344783813841E-5 0.0 62-63 0.3450008678965544 6.393344783813841E-5 0.0 64-65 0.38251381841558213 6.393344783813841E-5 0.0 66-67 0.4221046059893493 6.393344783813841E-5 0.0 68-69 0.4670977699054392 6.393344783813841E-5 0.0 70-71 0.5167900422376324 6.393344783813841E-5 0.0 72-73 0.5723162416850556 6.393344783813841E-5 0.0 74-75 0.6345554531554833 6.393344783813841E-5 0.0 76-77 0.7046904454339211 6.393344783813841E-5 0.0 78-79 0.7853584732436922 6.393344783813841E-5 0.0 80-81 0.8762878194314846 6.393344783813841E-5 0.0 82-83 0.9788530531258182 6.393344783813841E-5 0.0 84-85 1.0955635621543398 6.393344783813841E-5 0.0 86-87 1.2280336660749627 6.393344783813841E-5 0.0 88-89 1.3714843396617857 6.393344783813841E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1055 0.0 70.550575 9 AGAGCGG 1335 0.0 59.663563 8 GGATCGG 740 0.0 52.573772 1 GATCGGG 2420 0.0 46.464363 1 ATCGGGA 2255 0.0 45.440025 2 GAGAGCG 1955 0.0 44.139416 7 TTGTGTT 26690 0.0 42.591724 1 TCGGGAG 2110 0.0 38.89324 3 CGGGAGA 2055 0.0 38.54671 4 GAATCGG 485 0.0 37.173107 1 GGCGCCG 1275 0.0 36.701183 56-57 GATTGGG 2190 0.0 35.529305 1 TCGGGAA 765 0.0 34.72459 3 CGCCGGA 735 0.0 34.57576 58-59 TCGGAAA 1020 0.0 34.412346 4 AAGGGGC 1675 0.0 33.114975 9 TCTCGGG 1210 0.0 32.59371 48-49 ATTGGGA 1965 0.0 32.591366 2 CCTATGT 1225 0.0 31.033678 36-37 TCCGGAA 510 0.0 30.694057 3 >>END_MODULE