FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_30521-1.342000000356dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_30521-1.342000000356dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3128253
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA47790.15276897360923172No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC42780.13675364492577807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC10550.070.5505759
AGAGCGG13350.059.6635638
GGATCGG7400.052.5737721
GATCGGG24200.046.4643631
ATCGGGA22550.045.4400252
GAGAGCG19550.044.1394167
TTGTGTT266900.042.5917241
TCGGGAG21100.038.893243
CGGGAGA20550.038.546714
GAATCGG4850.037.1731071
GGCGCCG12750.036.70118356-57
GATTGGG21900.035.5293051
TCGGGAA7650.034.724593
CGCCGGA7350.034.5757658-59
TCGGAAA10200.034.4123464
AAGGGGC16750.033.1149759
TCTCGGG12100.032.5937148-49
ATTGGGA19650.032.5913662
CCTATGT12250.031.03367836-37
TCCGGAA5100.030.6940573
Produced by FastQC (version 0.11.2)