##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_30484-1.34200000035803.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8572676 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.670616152995866 33.0 33.0 33.0 27.0 33.0 2 31.428123610410566 33.0 33.0 33.0 27.0 33.0 3 31.35258931983432 33.0 33.0 33.0 27.0 33.0 4 34.9454263756148 37.0 37.0 37.0 33.0 37.0 5 34.97581816926243 37.0 37.0 37.0 33.0 37.0 6 35.22242284672837 37.0 37.0 37.0 33.0 37.0 7 34.92290085382907 37.0 37.0 37.0 33.0 37.0 8 35.009490968747684 37.0 37.0 37.0 33.0 37.0 9 34.74097119732508 37.0 37.0 37.0 33.0 37.0 10-11 34.69697046756462 37.0 37.0 37.0 33.0 37.0 12-13 34.69798881936049 37.0 37.0 37.0 33.0 37.0 14-15 36.36435565743999 40.0 37.0 40.0 33.0 40.0 16-17 36.30217192391267 40.0 37.0 40.0 33.0 40.0 18-19 36.26897313044375 40.0 37.0 40.0 33.0 40.0 20-21 36.16856271017358 40.0 37.0 40.0 27.0 40.0 22-23 36.5644967219104 40.0 37.0 40.0 33.0 40.0 24-25 36.15059883284986 40.0 37.0 40.0 27.0 40.0 26-27 36.06853606738433 40.0 37.0 40.0 27.0 40.0 28-29 35.94565390083564 40.0 37.0 40.0 27.0 40.0 30-31 35.88981386908826 40.0 37.0 40.0 27.0 40.0 32-33 35.73962838441579 40.0 37.0 40.0 27.0 40.0 34-35 35.84198778771062 40.0 37.0 40.0 27.0 40.0 36-37 35.615735040027175 40.0 37.0 40.0 27.0 40.0 38-39 35.55747254416241 40.0 37.0 40.0 27.0 40.0 40-41 35.74926230735887 37.0 37.0 40.0 27.0 40.0 42-43 35.53300853782413 37.0 37.0 40.0 27.0 40.0 44-45 35.192562625719205 37.0 37.0 40.0 24.5 40.0 46-47 35.06439057069228 37.0 37.0 40.0 22.0 40.0 48-49 34.93016567988805 37.0 37.0 40.0 22.0 40.0 50-51 33.83600482509779 37.0 35.0 38.5 22.0 38.5 52-53 34.245573727503526 37.0 35.0 38.5 22.0 40.0 54-55 34.96502404850014 37.0 37.0 40.0 24.5 40.0 56-57 35.09200604338715 37.0 37.0 40.0 24.5 40.0 58-59 34.75984266756378 37.0 35.0 40.0 22.0 40.0 60-61 34.493110494319396 37.0 33.0 40.0 22.0 40.0 62-63 34.18743219736754 37.0 33.0 40.0 22.0 40.0 64-65 33.955337574871606 37.0 33.0 38.5 22.0 40.0 66-67 33.768300120055855 37.0 33.0 37.0 22.0 40.0 68-69 33.6692970199737 37.0 33.0 37.0 22.0 40.0 70-71 33.28036053153065 37.0 33.0 37.0 22.0 40.0 72-73 33.23914096368509 37.0 33.0 37.0 22.0 38.5 74-75 33.13610254254331 37.0 33.0 37.0 22.0 37.0 76-77 32.885537374794055 37.0 33.0 37.0 22.0 37.0 78-79 32.56062605188858 37.0 33.0 37.0 22.0 37.0 80-81 32.21574255226723 37.0 33.0 37.0 22.0 37.0 82-83 31.92870289277234 37.0 33.0 37.0 18.5 37.0 84-85 31.717819558326944 33.0 33.0 37.0 15.0 37.0 86-87 31.554744749480793 33.0 33.0 37.0 15.0 37.0 88-89 31.3942871514099 33.0 33.0 37.0 15.0 37.0 90-91 31.2278365588528 33.0 33.0 37.0 15.0 37.0 92-93 31.088437612712763 33.0 33.0 37.0 15.0 37.0 94-95 30.962995335412188 33.0 33.0 37.0 15.0 37.0 96-97 30.82049805684946 33.0 33.0 37.0 15.0 37.0 98-99 30.675345773011834 33.0 27.0 37.0 15.0 37.0 100-101 29.38199583187327 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2102534075960385 2306 2 0.30379224287209183 2306 3 0.20263774679418134 2306 4 0.37793347364473817 2306 5 0.3193928833009423 2306 6 0.17858225515374926 2306 7 0.38325652845320235 2306 8 0.1903253455137488 2306 9 0.3204065717479878 2306 10-11 0.32529610261260444 2306 12-13 0.31368537523321294 2306 14-15 0.5336338620063046 2306 16-17 0.4962101616776451 2306 18-19 0.5676051242892086 2306 20-21 0.6179944911049304 2306 22-23 0.37993500307753436 2306 24-25 0.44214389565841117 2306 26-27 0.357677241997024 2306 28-29 0.37578380002290857 2306 30-31 0.34772056864978396 2306 32-33 0.3719166229141777 2306 34-35 0.20912029519224973 2306 36-37 0.3158001327003106 2306 38-39 0.11720431090206063 2306 40-41 0.006504630190939054 2306 42-43 0.16067901057265033 2306 44-45 0.2712949696536029 2306 46-47 0.1924779331348887 2306 48-49 0.21947018760144488 2306 50-51 0.11296330210902283 2306 52-53 0.148928043083302 2306 54-55 0.3273753262093564 2306 56-57 0.23657111655570162 2306 58-59 0.314593974853274 2306 60-61 0.44570114209205514 2306 62-63 0.5110265682503368 2306 64-65 0.4423768222484128 2306 66-67 0.33465941108939745 2306 68-69 0.15535751852057444 2306 70-71 0.3157323129313738 2306 72-73 0.05179939279727819 2306 74-75 0.023854758577790847 2306 76-77 0.06389746150332343 2306 78-79 0.27569235899338196 2306 80-81 0.2381307177913996 2306 82-83 0.1496687176459588 2306 84-85 0.15859441722533418 2306 86-87 0.24836088628776665 2306 88-89 0.32376734988536526 2306 90-91 0.1621211636537403 2306 92-93 0.20409511686319703 2306 94-95 0.27046338605875775 2306 96-97 0.20008103913760777 2306 98-99 0.01963278129449364 2306 100-101 -0.1335298980757038 2307 1 0.08275762055610159 2307 2 0.061146721106258184 2307 3 2.7349055934777766E-4 2307 4 0.13319616035988702 2307 5 0.05145829286048098 2307 6 -0.06102206871788951 2307 7 0.2350511285712784 2307 8 -0.002426191658422283 2307 9 0.03182718638741733 2307 10-11 0.07116191148659112 2307 12-13 0.10364834871236184 2307 14-15 0.33054021977583403 2307 16-17 0.20879532738740636 2307 18-19 0.34139164224340846 2307 20-21 0.3667570301155365 2307 22-23 0.15791628946335123 2307 24-25 0.1968854167818037 2307 26-27 0.1567244349344179 2307 28-29 0.11717131327227293 2307 30-31 0.07996621464313591 2307 32-33 0.06734962261138833 2307 34-35 -0.04029935496306081 2307 36-37 0.060402975429163064 2307 38-39 -0.06902605985891341 2307 40-41 -0.24195328097572855 2307 42-43 -0.06254915957480023 2307 44-45 0.03164061880180924 2307 46-47 0.04306549407582594 2307 48-49 -0.11959731932181938 2307 50-51 -0.18748849765358244 2307 52-53 -0.1516147557971692 2307 54-55 -0.11754541933604656 2307 56-57 -0.05915163809945767 2307 58-59 0.023205539730817293 2307 60-61 -0.036611841609634155 2307 62-63 -0.007994295991345268 2307 64-65 0.03992066550867435 2307 66-67 -0.04867082884754126 2307 68-69 -0.13802916873049043 2307 70-71 -0.14235945892100688 2307 72-73 -0.25002104025364247 2307 74-75 -0.29168871839156196 2307 76-77 -0.11878533671340819 2307 78-79 -0.01676200726392807 2307 80-81 0.026578992311279137 2307 82-83 -0.09022028063853327 2307 84-85 -0.1030740583612122 2307 86-87 -0.014766566150751004 2307 88-89 0.1068187744251361 2307 90-91 0.06678702972483563 2307 92-93 -0.08011393445563542 2307 94-95 -0.08118568102615598 2307 96-97 -0.0073402031949711954 2307 98-99 -0.02662234483208792 2307 100-101 -0.1537142481121485 2201 1 0.21967625381110167 2201 2 0.2405931709426632 2201 3 0.2682159893991134 2201 4 0.3985327601706885 2201 5 0.3181472732252004 2201 6 0.24270741798183337 2201 7 0.5102426351100462 2201 8 0.3223049786408012 2201 9 0.41695758593432686 2201 10-11 0.37198945526754557 2201 12-13 0.36567949956458534 2201 14-15 0.5681332225720155 2201 16-17 0.5806820047925427 2201 18-19 0.6365121865736754 2201 20-21 0.6452052906155572 2201 22-23 0.510217660748566 2201 24-25 0.6016609742949797 2201 26-27 0.40111126148492815 2201 28-29 0.4520987976275066 2201 30-31 0.45078807153020506 2201 32-33 0.41691992896761576 2201 34-35 0.23700339155875128 2201 36-37 0.43742548359730193 2201 38-39 0.3345381272755361 2201 40-41 0.23644876382676472 2201 42-43 0.39041844604918197 2201 44-45 0.5150528635985765 2201 46-47 0.44415491389643336 2201 48-49 0.3879060877784255 2201 50-51 0.3315460940018653 2201 52-53 0.40398392924420534 2201 54-55 0.4978865172992428 2201 56-57 0.5478588144734786 2201 58-59 0.6009480907908298 2201 60-61 0.49669216625594004 2201 62-63 0.5031749882735426 2201 64-65 0.5233753295751811 2201 66-67 0.5538624344455698 2201 68-69 0.41122843594050806 2201 70-71 0.44864575768069415 2201 72-73 0.41097972910407066 2201 74-75 0.34622070503952074 2201 76-77 0.35242565402253234 2201 78-79 0.46417977025455315 2201 80-81 0.549930161107433 2201 82-83 0.5421930775187889 2201 84-85 0.4241013916123286 2201 86-87 0.4809516443836408 2201 88-89 0.603258732370012 2201 90-91 0.4552732777462367 2201 92-93 0.3377972632243633 2201 94-95 0.4218931056975066 2201 96-97 0.33718426323260076 2201 98-99 0.4531818989543588 2201 100-101 0.2748185797539442 1101 1 0.14695073555817828 1101 2 0.14370310204445502 1101 3 0.1590339729616943 1101 4 0.24145987253900358 1101 5 0.23217615036913486 1101 6 0.17575319296949488 1101 7 0.4037097120229447 1101 8 0.187720228882732 1101 9 0.2845033319731627 1101 10-11 0.2694660825362334 1101 12-13 0.34013588195377054 1101 14-15 0.44496520082455504 1101 16-17 0.40347286468298194 1101 18-19 0.42216473448397807 1101 20-21 0.4713566268106959 1101 22-23 0.31194893843949956 1101 24-25 0.3565692478696647 1101 26-27 0.2527956007210648 1101 28-29 0.30624192614099854 1101 30-31 0.29454498133944895 1101 32-33 0.24217254194385873 1101 34-35 0.161330882743151 1101 36-37 0.26476613409121796 1101 38-39 0.266887200499518 1101 40-41 0.08503647463626152 1101 42-43 0.21899266119135063 1101 44-45 0.31821276588939185 1101 46-47 0.26160209607277096 1101 48-49 0.23850195112893147 1101 50-51 0.2725648583556577 1101 52-53 0.2499006972317659 1101 54-55 0.3739079973286934 1101 56-57 0.17193417516207177 1101 58-59 0.36653469051677945 1101 60-61 0.359283527374636 1101 62-63 0.39860775944823246 1101 64-65 0.389884225136079 1101 66-67 0.2272408865931581 1101 68-69 0.24232843621620503 1101 70-71 0.3138424442806951 1101 72-73 0.24993926691537638 1101 74-75 0.23302996535564802 1101 76-77 0.20803255928267106 1101 78-79 0.3913311505621877 1101 80-81 0.2965159916217033 1101 82-83 0.2782744816195972 1101 84-85 0.2933419536055588 1101 86-87 0.3665848930761193 1101 88-89 0.3695966920015614 1101 90-91 0.42254545737232974 1101 92-93 0.3951697712128599 1101 94-95 0.3657764943093156 1101 96-97 0.34275174294774047 1101 98-99 0.3548221946183787 1101 100-101 0.1499333411742576 2304 1 0.2017331468776895 2304 2 0.177615458704242 2304 3 0.2125561228785955 2304 4 0.27508789602903505 2304 5 0.278268372078351 2304 6 0.21634193444070604 2304 7 0.4222063711686985 2304 8 0.19650503489249616 2304 9 0.2942468852327593 2304 10-11 0.40583299670585404 2304 12-13 0.34627384863524924 2304 14-15 0.5257415925083748 2304 16-17 0.43672544739609975 2304 18-19 0.4833708975687827 2304 20-21 0.5774490759864435 2304 22-23 0.4548962900998603 2304 24-25 0.4633470004809297 2304 26-27 0.39748815307950736 2304 28-29 0.45917409078030147 2304 30-31 0.4551944321771515 2304 32-33 0.3613680315244423 2304 34-35 0.26517964072746736 2304 36-37 0.44012057051141795 2304 38-39 0.3535606621239822 2304 40-41 0.3009691261507541 2304 42-43 0.34462726862766146 2304 44-45 0.47603751792667026 2304 46-47 0.4324820963437972 2304 48-49 0.30752872829594224 2304 50-51 0.24852575927801723 2304 52-53 0.3529365043362702 2304 54-55 0.5034808006553178 2304 56-57 0.5188898501617629 2304 58-59 0.47324045238932655 2304 60-61 0.4720029962838268 2304 62-63 0.45455847761331825 2304 64-65 0.3809462421931684 2304 66-67 0.34060306945882957 2304 68-69 0.3365773804327077 2304 70-71 0.4583725032574364 2304 72-73 0.37478899381034125 2304 74-75 0.37363985637497166 2304 76-77 0.42321808002132855 2304 78-79 0.4584942750425185 2304 80-81 0.5256917932476526 2304 82-83 0.387447892427069 2304 84-85 0.3313271711845509 2304 86-87 0.3864019470650568 2304 88-89 0.39974254160843614 2304 90-91 0.3095144319047911 2304 92-93 0.38024639355742806 2304 94-95 0.44471282690991387 2304 96-97 0.40248058347821214 2304 98-99 0.37952530239202176 2304 100-101 0.29771387274903205 2202 1 0.05435865487474345 2202 2 0.008851794249657985 2202 3 0.03283549124330065 2202 4 0.0826264141974562 2202 5 0.045395830655245106 2202 6 -0.010821185690531365 2202 7 0.21119329663200404 2202 8 0.007731894268601991 2202 9 0.01408537452755354 2202 10-11 0.1319259846919678 2202 12-13 0.09814005513960211 2202 14-15 0.18373987766141653 2202 16-17 0.19795478855635906 2202 18-19 0.26711887919742594 2202 20-21 0.2587330852142884 2202 22-23 0.19468067357196617 2202 24-25 0.13166526155114866 2202 26-27 0.1593378304622064 2202 28-29 0.18657861749601778 2202 30-31 0.18392257710280546 2202 32-33 0.09284623487084787 2202 34-35 -0.048614657289270724 2202 36-37 0.08549832441331517 2202 38-39 0.08945435479037656 2202 40-41 -0.06594296627293517 2202 42-43 0.08475207381859207 2202 44-45 0.1710463059316467 2202 46-47 0.19593600192531113 2202 48-49 0.21531500026669192 2202 50-51 0.1222739224649132 2202 52-53 0.17649296250964142 2202 54-55 0.2715395139578547 2202 56-57 0.1877968577831055 2202 58-59 0.20382843463878686 2202 60-61 0.3095741407626136 2202 62-63 0.24306079628787103 2202 64-65 0.14622532065034477 2202 66-67 0.24640999045931977 2202 68-69 0.2121408332840744 2202 70-71 0.2576757075848519 2202 72-73 0.1996258402958162 2202 74-75 0.20408948830954188 2202 76-77 0.21264085509818642 2202 78-79 0.35975678572371095 2202 80-81 0.3022236763405317 2202 82-83 0.2639910643434007 2202 84-85 0.3333337558923084 2202 86-87 0.36970345645835323 2202 88-89 0.3557843975202921 2202 90-91 0.37725447387402866 2202 92-93 0.29535693031975185 2202 94-95 0.23322766431864395 2202 96-97 0.23140351551510463 2202 98-99 0.33210759513303145 2202 100-101 0.2157698626778206 1102 1 -0.0584896121659213 1102 2 -0.032031883426665075 1102 3 -0.027880022665723203 1102 4 0.05288706852898173 1102 5 0.05314325259480057 1102 6 0.023922973845031947 1102 7 0.13723584176652537 1102 8 -0.009567280187646077 1102 9 0.06237824009432558 1102 10-11 0.09461072955701155 1102 12-13 0.02945013413690134 1102 14-15 0.06329933499061013 1102 16-17 0.05140906633518938 1102 18-19 0.10009602629043712 1102 20-21 0.19983878487229134 1102 22-23 0.01872038584250646 1102 24-25 -0.06280082578887658 1102 26-27 -0.09254625542479289 1102 28-29 -0.06705771437007257 1102 30-31 -0.011802539929625766 1102 32-33 -0.07120526924111914 1102 34-35 -0.07005931947524857 1102 36-37 -0.008940141436454496 1102 38-39 -0.08885688517909074 1102 40-41 -0.09831589429200704 1102 42-43 -0.026742723792260392 1102 44-45 0.13841621174785956 1102 46-47 -0.07308804312749828 1102 48-49 -0.05406168434068803 1102 50-51 -0.052678012696539156 1102 52-53 -0.03267715075897826 1102 54-55 0.10067022702053663 1102 56-57 0.21030377012872492 1102 58-59 0.1936215105456327 1102 60-61 0.17556765631629645 1102 62-63 0.18610586583135813 1102 64-65 0.15666757294948042 1102 66-67 0.14832118101036684 1102 68-69 0.1159075366153175 1102 70-71 0.18963895648245455 1102 72-73 0.14550589854787432 1102 74-75 0.19034847370256358 1102 76-77 0.24168001608742173 1102 78-79 0.1809456517446293 1102 80-81 0.10530445283337997 1102 82-83 0.1527912432473073 1102 84-85 0.1530595525102001 1102 86-87 0.22710705770345996 1102 88-89 0.259815207254082 1102 90-91 0.34342017459522367 1102 92-93 0.2817963618962267 1102 94-95 0.2730577630308133 1102 96-97 0.17952317046551158 1102 98-99 0.18568381108567067 1102 100-101 0.13397354044602494 2305 1 0.11929270012050708 2305 2 0.07205048418142113 2305 3 0.10010242039704664 2305 4 0.16884122292954373 2305 5 0.12906480562787692 2305 6 0.18955087173822704 2305 7 0.24486328431198245 2305 8 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-0.959480306250164 1213 88-89 -1.1441070767418644 1213 90-91 -1.0863211899194205 1213 92-93 -1.084810178358449 1213 94-95 -1.1416669486412196 1213 96-97 -1.1127752551598888 1213 98-99 -1.0391551835783126 1213 100-101 -0.8717889583222309 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 800.0 3 460.0 4 556.0 5 534.0 6 1789.0 7 7377.0 8 27857.0 9 77283.0 10 113118.0 11 90882.0 12 54751.0 13 38300.0 14 29854.0 15 25622.0 16 24439.0 17 24919.0 18 26312.0 19 28355.0 20 30833.0 21 34346.0 22 38343.0 23 42104.0 24 48054.0 25 55522.0 26 65789.0 27 78846.0 28 96590.0 29 117130.0 30 142462.0 31 181823.0 32 245064.0 33 347056.0 34 519573.0 35 852323.0 36 1588718.0 37 2684685.0 38 828754.0 39 1453.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.427835460584454 27.927050049078723 17.31733908822612 17.327775402110706 2 32.34631926376511 26.927203262131478 25.363691551189323 15.36278592291409 3 34.403869664593635 22.659278742837984 24.245953376873487 18.690898215694897 4 30.651760590691985 21.07985995269455 26.535698231979865 21.7326812246336 5 33.32959118707032 19.866053749058135 25.928669474249865 20.875685589621675 6 31.98152762551803 19.84006088469214 27.72229721979995 20.45611426998988 7 33.55673570047362 20.809033713533054 23.226524769031005 22.407705816962327 8 29.117568078757 26.44883643818855 22.540898625582013 21.892696857472433 9 28.64850425292272 24.69264923232798 25.35674335155739 21.30210316319191 10-11 24.596218617049313 29.63934853348712 28.265195829892452 17.49923701957111 12-13 25.124607665798454 27.017619093708724 28.742938042748683 19.114835197744142 14-15 23.91340377952857 27.04691382673692 29.60519014547224 19.43449224826227 16-17 25.129291981848162 26.935375199219457 29.6556584162872 18.27967440264518 18-19 24.964258370826144 26.736203777837087 30.014702925945308 18.284834925391465 20-21 24.28432329326306 28.27753982642539 29.153975976515518 18.28416090379603 22-23 25.869362434561033 27.25915830252751 28.238094351214617 18.63338491169684 24-25 23.879598689638996 28.657661508508696 28.548849473697285 18.913890328155023 26-27 22.917754569190603 29.77430116885183 28.750204712840528 18.557739549117045 28-29 23.44285930605397 29.32690232909392 28.645495329484532 18.584743035367584 30-31 24.497229031335717 28.125096641474556 28.995903190687155 18.38177113650257 32-33 22.46136300891904 29.35305499226421 29.750806224443927 18.434775774372824 34-35 24.62577422745644 28.43088576871503 28.778388437517282 18.164951566311245 36-37 22.875324355030557 28.747332233044826 30.099111916671827 18.278231495252793 38-39 22.694067093954764 29.59922530006523 29.067639575755994 18.63906803022401 40-41 25.08368659747175 27.51398246448455 28.235119089912747 19.167211848130954 42-43 24.901383800181513 27.413405368663874 28.585463327233224 19.099747503921396 44-45 23.31663878068165 28.19300571540856 29.355181676071485 19.135173827838305 46-47 23.57637479592808 28.718516362106634 28.61141132225035 19.093697519714937 48-49 21.737051240153523 29.038116354325034 29.522676514877 19.702155890644445 50-51 21.352462434783565 28.267659398097884 29.94163612343882 20.43824204367973 52-53 24.01913529688084 27.932960599764645 28.036846916630605 20.011057186723914 54-55 24.755193532090694 27.823381576813706 28.079466922377755 19.341957968717846 56-57 24.921412417724078 27.50569181315284 28.174584769750826 19.39831099937226 58-59 24.222258623310204 27.74345568445972 28.167266495126704 19.86701919710337 60-61 22.114888715008373 27.960787897401605 27.959349612034217 21.9649737755558 62-63 23.22322881783997 27.964239612369745 28.13197458514957 20.680556984640713 64-65 21.955241240191974 28.45427326280725 29.34953030516061 20.24095519184017 66-67 21.41682541423556 28.140719941224322 28.90250948421959 21.539945160320524 68-69 21.040173229283813 27.847055250688157 31.17081086099992 19.94196065902811 70-71 20.773621597503826 30.045577856979 29.68326189711735 19.49753864839982 72-73 20.79072457004057 31.702340393019917 27.865514472940117 19.641420563999397 74-75 20.980802513581285 31.664037439939165 27.629879552106978 19.725280494372573 76-77 21.11342895704767 31.340560709810404 27.748158692197034 19.79785164094489 78-79 20.998080454858474 31.107324339520197 28.043956432002133 19.850638773619195 80-81 20.63599997520951 31.367357966581256 27.85068104265519 20.145961015554043 82-83 20.565535500144307 31.131512546190525 27.79346646575841 20.50948548790676 84-85 20.82319275598889 30.722357254481846 28.016736111968 20.43771387756126 86-87 20.873690967055726 30.394470699112308 28.619192904245537 20.112645429586433 88-89 21.121340124762156 30.71574207306996 28.226370200705503 19.936547601462376 90-91 21.394566329590013 30.24196158461715 27.832063987006784 20.531408098786056 92-93 21.204303594680802 30.37098458390287 27.66091157160757 20.76380024980875 94-95 20.85476916644667 30.393277435898273 27.659099460005244 21.092853937649817 96-97 20.83837466848192 30.698080483266704 27.736049350817886 20.727495497433495 98-99 21.24876832140187 30.3889870801505 27.578403520010554 20.78384107843708 100-101 21.504682690418495 30.30220544894885 27.131027618985243 21.06208424164741 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 190.0 1 263.5 2 343.5 3 332.0 4 329.5 5 438.0 6 599.5 7 880.5 8 1281.0 9 1750.5 10 2165.5 11 2560.5 12 2817.5 13 2805.5 14 2644.5 15 2338.5 16 2040.0 17 1808.5 18 1721.5 19 1865.5 20 2228.5 21 2854.5 22 3861.5 23 5422.0 24 7473.0 25 10117.5 26 13906.0 27 18955.0 28 24926.0 29 31782.0 30 39695.0 31 49169.5 32 65974.0 33 94645.5 34 137163.5 35 198838.5 36 271162.0 37 353133.0 38 440580.0 39 492037.5 40 529087.5 41 535011.0 42 502476.5 43 499150.0 44 494914.0 45 488285.5 46 508241.5 47 488593.0 48 410829.0 49 335744.5 50 278178.5 51 233249.0 52 194904.5 53 160401.5 54 134707.0 55 111832.0 56 87393.5 57 65340.0 58 49776.0 59 38881.0 60 30167.5 61 23247.0 62 17630.0 63 13399.5 64 10437.5 65 8153.0 66 6307.0 67 4929.5 68 3824.0 69 2951.5 70 2305.5 71 1813.0 72 1383.5 73 1092.0 74 872.0 75 670.5 76 552.0 77 460.0 78 373.5 79 308.0 80 263.5 81 212.0 82 181.5 83 164.5 84 152.0 85 134.0 86 111.5 87 98.0 88 82.5 89 76.5 90 68.5 91 59.5 92 49.0 93 36.5 94 26.0 95 25.0 96 21.5 97 14.5 98 9.5 99 6.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18681447893283265 2 0.17591939786363092 3 0.1488566697259992 4 0.1416127239615728 5 0.14611540200516152 6 0.12735813181321678 7 0.10213846878150999 8 0.08860710471269415 9 0.08894538881441454 10-11 0.06931907843011914 12-13 0.08857210980561962 14-15 0.09312728020982014 16-17 0.08600581662015455 18-19 0.08205139212073337 20-21 0.07989920533565015 22-23 0.10130442349623385 24-25 0.4459984256957804 26-27 0.28131239300307165 28-29 0.12379448377612778 30-31 0.23097221917636918 32-33 0.26225766610099344 34-35 0.24597920182682748 36-37 0.24825970327118393 38-39 0.26578631923100793 40-41 0.2656171771801477 42-43 0.2918400275479909 44-45 0.26807265315987683 46-47 0.2860891978187441 48-49 0.27027733230557177 50-51 0.2847302289273501 52-53 0.2845027620313657 54-55 0.26131280360998127 56-57 0.27732880608108834 58-59 0.26678367408263187 60-61 0.2429404773958563 62-63 0.23558571442569393 64-65 0.23386513149452984 66-67 0.24308628950866681 68-69 0.24770561724250395 70-71 0.2339759487002658 72-73 0.2259387850421502 74-75 0.23444254746125948 76-77 0.23557404945666907 78-79 0.23784288593200067 80-81 0.24506933424288985 82-83 0.27025983485203453 84-85 0.26434569555644005 86-87 0.26435152804095247 88-89 0.2623101584616052 90-91 0.25374223871285934 92-93 0.23018483376719243 94-95 0.22898334195763376 96-97 0.22421236962647367 98-99 0.24890710905206265 100-101 0.2570492574314018 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8572676.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.072084413349906 #Duplication Level Percentage of deduplicated Percentage of total 1 89.77604715704713 44.95273811544545 2 4.903777720252507 4.910847439055761 3 1.492368119972257 2.2417794743712944 4 0.8529365388937908 1.7083324149888157 5 0.4644177414815445 1.1627182177260604 6 0.3407451017827915 1.0237090499942063 7 0.2457260705709298 0.861281158373186 8 0.2122119072685883 0.8500714027416587 9 0.15253364908260778 0.6873909977466565 >10 1.2433340996050122 12.758636918153579 >50 0.19023028021584856 6.70232282377871 >100 0.10497507574165062 10.078417649280178 >500 0.011700050989960182 4.056424525421524 >1k 0.008878993985890149 7.543952021672902 >5k 9.406683909485316E-5 0.26165070853228095 >10k+ 2.342627040043129E-5 0.1997270827176852 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 17027 0.19861942758597198 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.00270627281376317 0.0 0.0 2 0.003161206605731979 0.0 0.0 3 0.0033711760481791218 0.0 0.0 4 0.003581145490626264 0.0 0.0 5 0.004036079282595073 0.0 1.1664969024841252E-5 6 0.004409358291389993 0.0 1.1664969024841252E-5 7 0.004922616928483008 0.0 1.1664969024841252E-5 8 0.005704169853147372 0.0 1.1664969024841252E-5 9 0.006427397932687529 0.0 1.1664969024841252E-5 10-11 0.009757746589279706 0.0 2.3329938049682503E-5 12-13 0.01267982133000244 0.0 2.3329938049682503E-5 14-15 0.016336789119290172 0.0 2.3329938049682503E-5 16-17 0.020320376041273458 0.0 2.3329938049682503E-5 18-19 0.025482124834765713 0.0 2.3329938049682503E-5 20-21 0.03161206605731979 0.0 2.3329938049682503E-5 22-23 0.03859355001868728 1.1664969024841252E-5 2.3329938049682503E-5 24-25 0.04700399268559782 1.1664969024841252E-5 2.916242256210313E-5 26-27 0.056102668524974 1.1664969024841252E-5 3.499490707452375E-5 28-29 0.06742352096358244 1.1664969024841252E-5 5.249236061178563E-5 30-31 0.10243009300713102 1.1664969024841252E-5 5.832484512420626E-5 32-33 0.11689465459793418 1.1664969024841252E-5 5.832484512420626E-5 34-35 0.13154002320862238 1.1664969024841252E-5 5.832484512420626E-5 36-37 0.14694944729043768 1.1664969024841252E-5 5.832484512420626E-5 38-39 0.16363035299596065 1.1664969024841252E-5 6.99898141490475E-5 40-41 0.18116280144029706 1.1664969024841252E-5 6.99898141490475E-5 42-43 0.20036334045518575 1.1664969024841252E-5 8.165478317388877E-5 44-45 0.22053207189913626 1.1664969024841252E-5 8.165478317388877E-5 46-47 0.24116156961956803 1.1664969024841252E-5 8.165478317388877E-5 48-49 0.2624093106983164 1.1664969024841252E-5 8.165478317388877E-5 50-51 0.2849460308543097 1.1664969024841252E-5 8.165478317388877E-5 52-53 0.30782686759653577 1.1664969024841252E-5 8.165478317388877E-5 54-55 0.3311684706152431 1.1664969024841252E-5 8.74872676863094E-5 56-57 0.35614900178194064 1.7497453537261876E-5 9.331975219873001E-5 58-59 0.3820219030790386 2.3329938049682503E-5 9.331975219873001E-5 60-61 0.4093879204113161 2.3329938049682503E-5 9.915223671115063E-5 62-63 0.43867282514818007 2.3329938049682503E-5 1.2831465927325378E-4 64-65 0.46989411474316767 2.3329938049682503E-5 1.2831465927325378E-4 66-67 0.5018561298712327 2.3329938049682503E-5 1.3414714378567438E-4 68-69 0.5351537839526421 2.3329938049682503E-5 1.39979628298095E-4 70-71 0.5719159338344293 2.3329938049682503E-5 1.39979628298095E-4 72-73 0.6109702501295978 2.3329938049682503E-5 1.5164459732293627E-4 74-75 0.6533082552052591 2.3329938049682503E-5 1.6330956634777753E-4 76-77 0.6985625025371307 2.3329938049682503E-5 1.6330956634777753E-4 78-79 0.7474737176582902 2.3329938049682503E-5 1.6330956634777753E-4 80-81 0.7997677737966535 2.3329938049682503E-5 1.808070198850394E-4 82-83 0.8575210354386424 2.3329938049682503E-5 1.9830447342230128E-4 84-85 0.9195261782901862 2.3329938049682503E-5 2.0996944244714252E-4 86-87 0.9884078203818738 2.3329938049682503E-5 2.0996944244714252E-4 88-89 1.0646208955056742 2.3329938049682503E-5 2.0996944244714252E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 4075 0.0 82.13549 9 CGGGAGA 6060 0.0 77.34319 4 ATTCGGA 960 0.0 73.15217 2 AGAGCGG 4750 0.0 72.860664 8 GATCGGG 8000 0.0 72.254105 1 CGGAAAG 2470 0.0 71.830666 5 ATCGGGA 7970 0.0 70.19273 2 TTCGGAG 1060 0.0 69.82033 3 ACCGGAA 615 0.0 67.11309 3 GAGAGCG 6450 0.0 65.49295 7 GTTCGGA 1120 0.0 65.24382 2 GATCCGG 1345 0.0 64.57048 1 TCGGGAA 1835 0.0 64.11782 3 AAGCGGC 1445 0.0 62.66417 9 TCGGAAA 2225 0.0 56.71388 4 AAAGCGG 1830 0.0 55.440426 8 GGACCGG 960 0.0 55.367126 1 GAACCGG 675 0.0 54.839825 1 AAGGGGC 4295 0.0 53.203182 9 ATCCGGA 1540 0.0 52.996166 2 >>END_MODULE