FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_30484-1.34200000035803.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_30484-1.34200000035803.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8572676
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT170270.19861942758597198Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC40750.082.135499
CGGGAGA60600.077.343194
ATTCGGA9600.073.152172
AGAGCGG47500.072.8606648
GATCGGG80000.072.2541051
CGGAAAG24700.071.8306665
ATCGGGA79700.070.192732
TTCGGAG10600.069.820333
ACCGGAA6150.067.113093
GAGAGCG64500.065.492957
GTTCGGA11200.065.243822
GATCCGG13450.064.570481
TCGGGAA18350.064.117823
AAGCGGC14450.062.664179
TCGGAAA22250.056.713884
AAAGCGG18300.055.4404268
GGACCGG9600.055.3671261
GAACCGG6750.054.8398251
AAGGGGC42950.053.2031829
ATCCGGA15400.052.9961662
Produced by FastQC (version 0.11.2)