##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_30423-1.34200000035748.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2681201 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.97526407009396 33.0 33.0 33.0 27.0 33.0 2 30.401066164006355 33.0 33.0 33.0 22.0 33.0 3 30.25912454903605 33.0 33.0 33.0 22.0 33.0 4 33.589932272888156 37.0 37.0 37.0 15.0 37.0 5 33.69571956746249 37.0 37.0 37.0 22.0 37.0 6 34.349486293642286 37.0 37.0 37.0 27.0 37.0 7 33.71934144437511 37.0 37.0 37.0 22.0 37.0 8 33.97401873265003 37.0 37.0 37.0 27.0 37.0 9 33.38112435434717 37.0 37.0 37.0 15.0 37.0 10-11 33.38972087508546 37.0 37.0 37.0 15.0 37.0 12-13 33.373632375938996 37.0 37.0 37.0 15.0 37.0 14-15 34.87003417498352 40.0 37.0 40.0 15.0 40.0 16-17 34.73651751584458 40.0 37.0 40.0 15.0 40.0 18-19 34.83587261827815 40.0 37.0 40.0 15.0 40.0 20-21 34.7282520034865 40.0 37.0 40.0 15.0 40.0 22-23 35.62883871817145 40.0 37.0 40.0 24.5 40.0 24-25 34.8980855967158 40.0 37.0 40.0 18.5 40.0 26-27 34.732649100160714 38.5 37.0 40.0 18.5 40.0 28-29 34.49350981146135 37.0 37.0 40.0 15.0 40.0 30-31 34.48054342065365 37.0 37.0 40.0 15.0 40.0 32-33 34.28203816125684 37.0 37.0 40.0 15.0 40.0 34-35 34.553567226030424 37.0 37.0 40.0 18.5 40.0 36-37 34.12081749932213 37.0 37.0 40.0 15.0 40.0 38-39 34.04916472133197 37.0 35.0 40.0 15.0 40.0 40-41 34.62921131239321 37.0 33.0 40.0 18.5 40.0 42-43 34.27214184986504 37.0 33.0 40.0 15.0 40.0 44-45 33.685235086813705 37.0 33.0 40.0 15.0 40.0 46-47 33.522134297279464 37.0 33.0 40.0 10.5 40.0 48-49 33.40574447794104 37.0 33.0 40.0 10.5 40.0 50-51 32.4199384902512 37.0 33.0 38.5 15.0 38.5 52-53 32.87901541137722 37.0 33.0 38.5 15.0 40.0 54-55 33.56217866545626 37.0 33.0 40.0 15.0 40.0 56-57 33.854318270058826 37.0 33.0 40.0 18.5 40.0 58-59 33.294186262051966 37.0 33.0 40.0 15.0 40.0 60-61 33.06673352725141 37.0 33.0 40.0 15.0 40.0 62-63 32.699081866670944 37.0 33.0 38.5 6.0 40.0 64-65 32.469814646496104 37.0 33.0 37.0 6.0 40.0 66-67 32.31807704830783 37.0 33.0 37.0 10.5 40.0 68-69 32.428864154533734 37.0 33.0 37.0 15.0 40.0 70-71 31.992168621449864 37.0 33.0 37.0 15.0 37.0 72-73 32.26900370393716 37.0 33.0 37.0 18.5 37.0 74-75 32.40924272368987 37.0 33.0 37.0 22.0 37.0 76-77 32.10542551640105 37.0 33.0 37.0 22.0 37.0 78-79 31.692668136406034 35.0 33.0 37.0 18.5 37.0 80-81 31.26243985437869 33.0 33.0 37.0 15.0 37.0 82-83 30.780689884868757 33.0 30.0 37.0 15.0 37.0 84-85 30.569263736661295 33.0 30.0 37.0 15.0 37.0 86-87 30.438838043100837 33.0 27.0 37.0 15.0 37.0 88-89 30.36313763869251 33.0 27.0 37.0 15.0 37.0 90-91 30.250670688247542 33.0 27.0 37.0 15.0 37.0 92-93 30.065085758210593 33.0 27.0 37.0 6.0 37.0 94-95 29.891615175438172 33.0 27.0 37.0 6.0 37.0 96-97 29.75914431629706 33.0 27.0 37.0 6.0 37.0 98-99 29.6368720584544 33.0 27.0 37.0 6.0 37.0 100-101 28.386439323273414 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.125285208332496 2306 2 -0.07044052483347585 2306 3 -0.10024547118266369 2306 4 0.007142555011796503 2306 5 -0.04640631722597277 2306 6 -0.36379416375735474 2306 7 0.2982872312284499 2306 8 -0.2573795322993888 2306 9 0.019738224187278774 2306 10-11 0.06761840169002653 2306 12-13 -0.008299128920320697 2306 14-15 0.35049919625346604 2306 16-17 0.2679299600127507 2306 18-19 0.26382842792398975 2306 20-21 0.253439624537549 2306 22-23 0.027675556563046655 2306 24-25 0.17361932963488158 2306 26-27 -0.1396824199347222 2306 28-29 -0.06973568520847095 2306 30-31 0.1343809602679258 2306 32-33 0.12626933891650793 2306 34-35 -0.1321538916993319 2306 36-37 0.22500414946046732 2306 38-39 0.03017938052808944 2306 40-41 -0.3347706935796424 2306 42-43 0.027469475009112898 2306 44-45 0.2903996465087033 2306 46-47 0.2627042003391864 2306 48-49 0.10009777270820308 2306 50-51 0.02384589223812128 2306 52-53 0.10466093434359891 2306 54-55 0.2654556606803382 2306 56-57 0.0867082130646537 2306 58-59 0.24941471280107663 2306 60-61 0.2615320821538134 2306 62-63 0.4029130892708821 2306 64-65 0.31964355576936043 2306 66-67 0.13893077023364242 2306 68-69 -0.06217433558934715 2306 70-71 0.11384015053616281 2306 72-73 -0.2885277323889319 2306 74-75 -0.3198155729528622 2306 76-77 -0.24986113573373814 2306 78-79 -0.016872786176609367 2306 80-81 0.05657624791528448 2306 82-83 0.19394248976894346 2306 84-85 0.17776499385228206 2306 86-87 0.24156798101969912 2306 88-89 0.27934057003886537 2306 90-91 0.15733700217940338 2306 92-93 0.25046106384926503 2306 94-95 0.2250203444433616 2306 96-97 0.2560610993682779 2306 98-99 0.29179785760495136 2306 100-101 -0.15999371486969594 2307 1 -0.18520496548643095 2307 2 -0.07591887774577089 2307 3 -0.1429484809290038 2307 4 -0.009055438672092464 2307 5 -0.1237962173837559 2307 6 -0.334627541319243 2307 7 0.19635551848379862 2307 8 -0.3750332033919008 2307 9 -0.010659993388358657 2307 10-11 0.048621897242803414 2307 12-13 -0.024276932445921773 2307 14-15 0.20615842354114733 2307 16-17 0.1036701432468945 2307 18-19 0.2249105659295978 2307 20-21 0.29246254636024105 2307 22-23 0.07055071745291741 2307 24-25 0.2724061880112387 2307 26-27 -0.12821062155813934 2307 28-29 0.23576536715894747 2307 30-31 0.19499476551040829 2307 32-33 0.12232306313772767 2307 34-35 -0.11179691691069849 2307 36-37 0.08625223300317231 2307 38-39 -0.06256150246982628 2307 40-41 -0.3181351774242387 2307 42-43 -0.013298229273061679 2307 44-45 0.18213045818841067 2307 46-47 0.08903418019545484 2307 48-49 0.03715305459106588 2307 50-51 -0.054913460059871966 2307 52-53 -0.08267012958856412 2307 54-55 -0.05542781082299797 2307 56-57 -0.00912254480591912 2307 58-59 0.24973397492180993 2307 60-61 0.2347004552261609 2307 62-63 0.41692714496946337 2307 64-65 0.32095721188676407 2307 66-67 0.042259261560893435 2307 68-69 -0.14970087612433502 2307 70-71 -0.11058084324816164 2307 72-73 -0.2248206500772767 2307 74-75 -0.25572295373241616 2307 76-77 -0.11569497191171507 2307 78-79 0.15548684395836077 2307 80-81 0.2567785016935602 2307 82-83 0.1476847290184793 2307 84-85 0.19160485708942687 2307 86-87 0.26400220091100834 2307 88-89 0.3052589113347004 2307 90-91 0.2883893396653221 2307 92-93 0.17450743497120058 2307 94-95 0.0982674307342215 2307 96-97 0.26095563535902144 2307 98-99 0.21164510170330075 2307 100-101 0.13402614905877286 2201 1 0.06931310358859122 2201 2 0.052484613353612986 2201 3 0.07840142292597463 2201 4 0.24854065833087446 2201 5 0.27191231060501053 2201 6 -0.0019169453054743713 2201 7 0.4960286680922792 2201 8 0.17306091628891096 2201 9 0.35015248006772026 2201 10-11 0.35295477243781903 2201 12-13 0.42641099532473703 2201 14-15 0.5122423973077517 2201 16-17 0.4491654395037443 2201 18-19 0.5132459925424584 2201 20-21 0.6259134377024935 2201 22-23 0.47698312355412753 2201 24-25 0.6878912721941575 2201 26-27 0.34384436357726145 2201 28-29 0.5208126820344319 2201 30-31 0.5250178894019726 2201 32-33 0.6293930836045618 2201 34-35 0.4652179402163128 2201 36-37 0.6466309867673417 2201 38-39 0.5860679240960778 2201 40-41 0.3271015623524818 2201 42-43 0.6158432895835375 2201 44-45 0.8315702080192793 2201 46-47 0.8003547496813113 2201 48-49 0.7608655009429981 2201 50-51 0.5610912180730594 2201 52-53 0.6214806462942946 2201 54-55 0.7774536533557708 2201 56-57 0.8335185461466281 2201 58-59 0.9892237820555394 2201 60-61 0.7658400778596146 2201 62-63 0.7740520139838765 2201 64-65 0.7493419755151365 2201 66-67 0.7249381382541102 2201 68-69 0.5573629285294714 2201 70-71 0.7624482371529808 2201 72-73 0.4160995676949284 2201 74-75 0.39931182814323307 2201 76-77 0.4964254900112266 2201 78-79 0.6306441333730213 2201 80-81 0.7671651786185407 2201 82-83 0.7553071298482514 2201 84-85 0.6037854229898478 2201 86-87 0.7372608662345641 2201 88-89 0.7107147945350363 2201 90-91 0.8479573700048917 2201 92-93 0.7381089410653665 2201 94-95 0.6342915352836656 2201 96-97 0.6734173800977281 2201 98-99 0.5841071558987849 2201 100-101 0.5163994905304676 1101 1 -0.11073734441984229 1101 2 -0.19235450518666042 1101 3 -0.20522535383949503 1101 4 -0.06838870609114878 1101 5 -0.08823982820648979 1101 6 -0.35094835190972873 1101 7 0.19270478853167106 1101 8 -0.4537900989458876 1101 9 -0.2087568641081745 1101 10-11 -0.1559133683481022 1101 12-13 -0.13856852847283818 1101 14-15 0.006729090913488278 1101 16-17 -0.03822935590154941 1101 18-19 0.09155387180188512 1101 20-21 0.017177273257047432 1101 22-23 -0.0610039967263063 1101 24-25 0.018979857391052235 1101 26-27 -0.19964194518463074 1101 28-29 -0.023762274670630745 1101 30-31 -0.0013846990911972057 1101 32-33 -0.16389452860670417 1101 34-35 -0.3991083948153573 1101 36-37 0.02312853129628678 1101 38-39 -0.15782895448568723 1101 40-41 -0.44370562669787716 1101 42-43 0.02320834325252008 1101 44-45 0.05929266449427928 1101 46-47 -0.09935996390284174 1101 48-49 -0.10295415788456097 1101 50-51 -0.12718422136005358 1101 52-53 -0.051986671260443984 1101 54-55 0.04203431997224527 1101 56-57 -0.010128706821269873 1101 58-59 0.10694887387425922 1101 60-61 0.18015166751568046 1101 62-63 0.1165170224755343 1101 64-65 0.08118822203272202 1101 66-67 -0.06750605938830034 1101 68-69 -0.06621220804827033 1101 70-71 0.12482786887414932 1101 72-73 -0.0762537524198521 1101 74-75 0.060960361135229846 1101 76-77 0.11866674527145449 1101 78-79 0.23120550341711166 1101 80-81 0.22176772277100554 1101 82-83 0.24030240296188765 1101 84-85 0.17369471278562898 1101 86-87 0.31177102694869774 1101 88-89 0.12965601382073544 1101 90-91 0.28989300905482907 1101 92-93 0.3331690310853652 1101 94-95 0.1858935523673857 1101 96-97 0.14176554246836304 1101 98-99 0.33332648760908157 1101 100-101 0.15721573596044536 2304 1 0.0414039257862413 2304 2 -0.014765700569583373 2304 3 0.03923271029220743 2304 4 0.23652161678056416 2304 5 0.13438162109847696 2304 6 0.02583901923526355 2304 7 0.589048328118146 2304 8 -0.014184899630279801 2304 9 0.16906049588569516 2304 10-11 0.28011006602628896 2304 12-13 0.299587663803095 2304 14-15 0.4965579510307876 2304 16-17 0.36782395666175205 2304 18-19 0.5305738501806303 2304 20-21 0.5668152875066426 2304 22-23 0.28172734643022324 2304 24-25 0.3541710011894139 2304 26-27 0.2418127650990911 2304 28-29 0.4179503584405282 2304 30-31 0.43143832158519047 2304 32-33 0.1978011650184328 2304 34-35 -0.055168091607072256 2304 36-37 0.3012929805076183 2304 38-39 0.15742594647365138 2304 40-41 0.048186190423777475 2304 42-43 0.2886807335927557 2304 44-45 0.40753657606845906 2304 46-47 0.30378916316033155 2304 48-49 0.16990414629412243 2304 50-51 0.08053665166190171 2304 52-53 0.21902361160343276 2304 54-55 0.510919506008662 2304 56-57 0.2886404139427938 2304 58-59 0.4021398474265325 2304 60-61 0.43333590783506537 2304 62-63 0.39721579755492087 2304 64-65 0.4060177631343791 2304 66-67 0.10955051338763866 2304 68-69 0.1565024509913755 2304 70-71 0.36623639990575896 2304 72-73 0.19611062528812795 2304 74-75 0.20708704976364345 2304 76-77 0.2986192040506239 2304 78-79 0.3085706472324965 2304 80-81 0.5474116786091727 2304 82-83 0.2594523216693787 2304 84-85 0.4208284070406769 2304 86-87 0.32356163276941174 2304 88-89 0.4362892271305725 2304 90-91 0.21977034674646134 2304 92-93 0.405703867691674 2304 94-95 0.2963839036701934 2304 96-97 0.3409345287380141 2304 98-99 0.4162788953144201 2304 100-101 0.39811416714271886 2202 1 -0.046335834315467395 2202 2 -0.1388642394379005 2202 3 -0.07130475769371714 2202 4 -0.033094951310388865 2202 5 -0.08654959700260179 2202 6 -0.1960355777098144 2202 7 0.2833641020757298 2202 8 -0.13905177634613608 2202 9 -0.062421087958938415 2202 10-11 -0.07813620744302341 2202 12-13 0.008974467554502041 2202 14-15 0.12570964708591248 2202 16-17 0.033140965119002885 2202 18-19 0.12487856891738858 2202 20-21 0.2317508908453192 2202 22-23 0.0448673976527445 2202 24-25 0.07880663953628897 2202 26-27 -0.033728480087283685 2202 28-29 0.15290328162517142 2202 30-31 0.17134582623759087 2202 32-33 0.09662746907385866 2202 34-35 -0.17362846785308506 2202 36-37 0.060902731212387096 2202 38-39 -0.0261303338130503 2202 40-41 -0.16953717110037303 2202 42-43 0.16983158208095261 2202 44-45 0.13295713509412366 2202 46-47 0.06665651893575841 2202 48-49 0.003819721257734443 2202 50-51 -0.09187504476173558 2202 52-53 -0.02319167039682668 2202 54-55 0.17138327252239804 2202 56-57 0.1558902435081464 2202 58-59 0.16397998893335597 2202 60-61 0.2324791282208949 2202 62-63 0.1565224509553218 2202 64-65 0.22622486427122368 2202 66-67 0.12662069561452682 2202 68-69 0.009565668429146967 2202 70-71 0.24971621491506113 2202 72-73 -0.010577783312839983 2202 74-75 0.18937290092351589 2202 76-77 0.16392607855544128 2202 78-79 0.3403691474075323 2202 80-81 0.31694809634911536 2202 82-83 0.18125966602108434 2202 84-85 0.22286625274664473 2202 86-87 0.3523964124941372 2202 88-89 0.37946354953766104 2202 90-91 0.48110010555839366 2202 92-93 0.328597387489733 2202 94-95 0.2819955337625011 2202 96-97 0.4002678989220847 2202 98-99 0.399118428784103 2202 100-101 0.1184557734663052 1102 1 -0.13357954626736301 1102 2 -0.12417744305301781 1102 3 -0.13516451335623714 1102 4 -0.039411289201609634 1102 5 -0.06832466913741087 1102 6 -0.17001345360309728 1102 7 0.15332615559901086 1102 8 -0.053500805782448424 1102 9 0.05420268774038561 1102 10-11 0.10004199664497548 1102 12-13 0.11532656174538403 1102 14-15 0.13209741461520963 1102 16-17 0.0744068874573145 1102 18-19 0.15243804798709704 1102 20-21 0.1925994849009811 1102 22-23 -0.029187881088766687 1102 24-25 -0.022582463669174047 1102 26-27 -0.08687768921766548 1102 28-29 0.041545139559005406 1102 30-31 0.1973935853712092 1102 32-33 0.07840104790611946 1102 34-35 0.03886441821953923 1102 36-37 0.24055075980415808 1102 38-39 -0.011374098860144954 1102 40-41 -0.048727419502952785 1102 42-43 0.09471622445988714 1102 44-45 0.15131322469891018 1102 46-47 0.07805132987536467 1102 48-49 0.18913889808300866 1102 50-51 -0.024235159646472937 1102 52-53 0.15674871364740284 1102 54-55 0.2727125606650773 1102 56-57 0.3627122302310255 1102 58-59 0.37426660346717 1102 60-61 0.3852001743290927 1102 62-63 0.3606711772512128 1102 64-65 0.3570469605029558 1102 66-67 0.227594909598146 1102 68-69 0.10273837218612414 1102 70-71 0.203103937645281 1102 72-73 0.20133852205757563 1102 74-75 0.40335336520786313 1102 76-77 0.3563396868555486 1102 78-79 0.31466924480404757 1102 80-81 0.2110335398018961 1102 82-83 0.4379639307272072 1102 84-85 0.4372062710976756 1102 86-87 0.40224882442662135 1102 88-89 0.3315495272609077 1102 90-91 0.38363407404307637 1102 92-93 0.30398436025023656 1102 94-95 0.49959659301176984 1102 96-97 0.4487544367997067 1102 98-99 0.3474621073855708 1102 100-101 0.23063987775855566 2305 1 0.12322083870921219 2305 2 0.2412351767808154 2305 3 0.3128859441924945 2305 4 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-0.8264755731556939 1213 86-87 -0.8066586548282828 1213 88-89 -1.0254994970370888 1213 90-91 -0.8734549752016356 1213 92-93 -0.9548176465074185 1213 94-95 -1.0053333382845828 1213 96-97 -0.9549767999410932 1213 98-99 -0.8900696708692095 1213 100-101 -0.6953803952025659 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 272.0 3 171.0 4 252.0 5 242.0 6 581.0 7 3132.0 8 15942.0 9 51013.0 10 75777.0 11 56161.0 12 30559.0 13 18716.0 14 13634.0 15 10864.0 16 9850.0 17 9098.0 18 9140.0 19 9228.0 20 9547.0 21 10076.0 22 10889.0 23 11317.0 24 13095.0 25 14970.0 26 17955.0 27 21838.0 28 26491.0 29 32312.0 30 39280.0 31 51662.0 32 71629.0 33 103339.0 34 157200.0 35 273462.0 36 532723.0 37 801871.0 38 166858.0 39 55.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.143857824052745 20.460006942739586 24.599082945560028 11.79705228764764 2 34.694068942841554 23.448113524783107 31.40235837424619 10.455459158129141 3 39.90320270766463 16.681489693669477 29.495719500765926 13.919588097899963 4 31.75530236110774 14.074652314139568 35.57117218737219 18.598873137380505 5 35.57205289108023 12.714178992977738 35.235619303750184 16.478148812191844 6 31.407341070813704 13.077892909666572 40.99948388580955 14.515282133710175 7 39.60105003731732 15.40736736110359 28.82801214614351 16.16357045543558 8 30.94246773555907 31.85846390238931 21.66345680385766 15.53561155819396 9 36.179251639858634 28.058185630018922 19.515913656323857 16.246649073798586 10-11 31.455313024741976 28.23774779391429 24.742348074659994 15.56459110668374 12-13 33.433472838022624 21.786380504732968 24.772684257621894 20.007462399622515 14-15 29.160102351057855 22.572428978460582 26.108162250468897 22.159306420012665 16-17 29.937011903848664 25.023055692586286 27.35888058989723 17.68105181366782 18-19 29.600605611876475 25.182763279628706 27.26619654352546 17.95043456496936 20-21 27.463552454715156 28.178001610310165 26.72973030477565 17.628715630199025 22-23 31.739995952907375 24.171167377147963 25.80575971470237 18.28307695524229 24-25 28.49291938906 27.331133877409275 26.109688451620116 18.066258281910606 26-27 23.836722419810364 29.563092289100478 27.492948727778817 19.107236563310344 28-29 25.197297765589266 28.845485994416585 25.523015520931825 20.43420071906232 30-31 26.805886615532504 26.56911214620506 26.612362550532787 20.012638687729652 32-33 23.657239033065522 26.871223730174933 29.12109341404333 20.350443822716212 34-35 29.076206737849436 24.44325760801821 26.214327454442742 20.26620819968961 36-37 23.959412916261005 28.25826539905872 28.855597230883813 18.926724453796467 38-39 22.867487856045056 30.67198420444474 24.983415414876394 21.477112524633814 40-41 29.43481310171741 25.159540245874556 23.888378761472957 21.51726789093507 42-43 30.579329268954105 25.607234939148395 24.701315256458738 19.112120535438763 44-45 24.962977893688965 28.964656229175066 25.569916060788056 20.502449816347916 46-47 25.6198495481301 29.3767888951746 25.734974683130034 19.26838687356526 48-49 22.51617501816546 27.57574613009348 28.72343289961859 21.18464595212247 50-51 19.870539497926792 26.415582686336275 29.155897927689427 24.557979888047505 52-53 26.133948426364345 27.02085009033587 25.40234104637004 21.442860436929745 54-55 27.833954221023554 26.63407201050757 25.95517455747145 19.576799210997425 56-57 29.046733935209772 25.997060429491448 24.110499427793528 20.845706207505255 58-59 26.193518567340195 24.811836069362347 25.09576008056449 23.89888528273297 60-61 21.458562052846148 24.84218704113951 26.764748821812162 26.934502084202176 62-63 24.96020025761186 24.88072227022927 26.987337541629213 23.171739930529657 64-65 23.141492077335627 28.12279031654209 27.89105334156008 20.844664264562205 66-67 21.0259298591077 27.843086526765724 26.766808479297406 24.364175134829175 68-69 19.948714420793 24.107138517425394 34.0754294704578 21.868717591323815 70-71 19.628254692003207 30.817959677435937 29.746409677594816 19.80737595296604 72-73 21.43490198057367 34.51782740581669 25.313514513678236 18.73375609993141 74-75 22.554581675689782 33.45200079851004 25.188786317066807 18.804631208733365 76-77 21.624260712829752 32.29663189167234 25.186521585288148 20.892585810209766 78-79 21.860157083271183 33.37531641523548 25.09021876438145 19.674307737111885 80-81 21.10724961813467 33.90102327382945 24.882664389027628 20.109062719008246 82-83 20.772188990082217 32.85253051147994 25.78910257141409 20.586177927023755 84-85 21.354236586514137 31.5701783678291 26.060628383964808 21.014956661691958 86-87 19.60293642244905 31.95985425957195 27.1557002049019 21.281509113077092 88-89 19.45797463677884 32.046830793944956 27.194789149620867 21.300405419655338 90-91 21.00931644795302 30.705335855209835 26.099178395966145 22.186169300870997 92-93 21.170377146155282 31.092180171720457 25.06631986388564 22.671122818238622 94-95 21.522921842419855 32.253917000045604 24.912621199536172 21.310539957998365 96-97 20.813939753685965 33.21320278519963 23.91604031571975 22.056817145394657 98-99 21.205066672200175 30.898590844179285 25.462931026908354 22.43341145671219 100-101 22.60130335508209 31.465815486843713 25.00224816318991 20.93063299488428 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 139.0 1 144.0 2 150.5 3 189.5 4 211.0 5 206.0 6 265.5 7 360.0 8 475.0 9 612.5 10 732.5 11 872.5 12 995.0 13 959.0 14 846.0 15 796.5 16 747.0 17 664.5 18 605.5 19 587.0 20 621.5 21 663.5 22 678.5 23 735.5 24 887.0 25 1101.0 26 1399.0 27 1722.5 28 2074.0 29 2549.0 30 3203.0 31 4457.5 32 6875.0 33 10900.0 34 17674.5 35 29067.5 36 45793.5 37 67222.5 38 92449.0 39 110941.5 40 119103.0 41 121602.5 42 151693.5 43 189586.5 44 182372.5 45 162164.5 46 162049.5 47 156561.0 48 133953.0 49 116950.5 50 103861.5 51 97459.0 52 97535.5 53 100153.0 54 87153.0 55 58414.5 56 42076.0 57 35641.0 58 31158.0 59 25832.5 60 20471.5 61 16397.0 62 12910.5 63 10053.0 64 7999.0 65 6210.0 66 4799.0 67 3785.0 68 2898.5 69 2156.5 70 1696.0 71 1329.0 72 958.5 73 674.5 74 466.0 75 332.5 76 273.0 77 212.5 78 159.0 79 120.0 80 86.0 81 67.5 82 60.0 83 46.0 84 31.5 85 37.5 86 31.5 87 22.5 88 19.0 89 10.5 90 9.0 91 11.0 92 12.0 93 8.5 94 5.5 95 6.0 96 4.0 97 1.0 98 1.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18737871573224088 2 0.17831561304057397 3 0.15142467871673926 4 0.14381614806200654 5 0.14922417230189008 6 0.1305012194162243 7 0.10517674728601101 8 0.09242126942366499 9 0.09189911535912451 10-11 0.07116213965308829 12-13 0.09092940066783506 14-15 0.0961136445943441 16-17 0.08807620167231028 18-19 0.08419734290715243 20-21 0.08257493563518736 22-23 0.10200652617987238 24-25 0.44399879009443904 26-27 0.2823361620408168 28-29 0.12192297407020213 30-31 0.226819995964495 32-33 0.2586527455420164 34-35 0.2420557056334083 36-37 0.24455458579942346 38-39 0.26223323055600833 40-41 0.2622891756343519 42-43 0.28830363706413653 44-45 0.26566452869441715 46-47 0.2830261513403881 48-49 0.26741747448251735 50-51 0.2822242718841296 52-53 0.28224292024357744 54-55 0.25878328405815154 56-57 0.27379521341369034 58-59 0.2636878025929425 60-61 0.24006033117248576 62-63 0.23297395458229353 64-65 0.23069885472965287 66-67 0.23972466070242404 68-69 0.24503944314506818 70-71 0.23114641535640185 72-73 0.22251222493203607 74-75 0.23194829481266044 76-77 0.23282476770671054 78-79 0.23549148310775658 80-81 0.24248461790070944 82-83 0.2672869359663822 84-85 0.2613754060214061 86-87 0.26087190031631347 88-89 0.2586527455420164 90-91 0.25128664356010605 92-93 0.22741674346682697 94-95 0.22627919354050666 96-97 0.22068468570614436 98-99 0.24561754228795232 100-101 0.2534498532560595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2681201.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.58847220341589 #Duplication Level Percentage of deduplicated Percentage of total 1 87.5803856148031 37.29914818320487 2 5.3110718541292705 4.523808720598579 3 2.0894683329466006 2.669617920528421 4 1.1379357612223238 1.9385178214435934 5 0.6584126884406949 1.402039524001643 6 0.46010761994781724 1.1757168349636475 7 0.3636343032914136 1.0840640592554411 8 0.25439845734576544 0.8667553303409612 9 0.20471112843792041 0.7846500782887446 >10 1.526680286843628 13.074730168517048 >50 0.21368486216448096 6.4603997726334805 >100 0.1763951102307983 15.06885987126969 >500 0.015943489313047596 4.655536138337475 >1k 0.006368745336645506 4.486723674968478 >5k 3.5633135454534777E-4 0.9875906281772012 >10k+ 4.454141917418566E-4 3.5218412734709235 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 31856 1.1881242771429668 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 24515 0.9143290637292766 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 12307 0.45901071944997784 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 12278 0.45792911460200114 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 11873 0.44282394344922293 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 9705 0.36196465688324003 Illumina Single End PCR Primer 1 (97% over 34bp) TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 6095 0.22732350166958762 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5150 0.1920781023131052 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 5081 0.18950462870929854 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3688 0.13755029928752077 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3504 0.13068770301070304 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2704 0.10085032789410417 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004177232516323842 0.0 0.0 2 0.004587496424177076 0.0 0.0 3 0.004960463613134561 0.0 0.0 4 0.005146947207613305 0.0 0.0 5 0.0059301783044240245 0.0 0.0 6 0.006713409401234745 0.0 0.0 7 0.007757717530315705 0.0 0.0 8 0.008764728940500918 0.0 0.0 9 0.009883630507373375 0.0 0.0 10-11 0.015347599825600543 0.0 0.0 12-13 0.020308063438735105 0.0 0.0 14-15 0.025734736038066523 0.0 0.0 16-17 0.02993061691383824 0.0 0.0 18-19 0.034667300213598304 0.0 0.0 20-21 0.03975830234286799 0.0 0.0 22-23 0.04462552415876318 0.0 0.0 24-25 0.04984706480416798 0.0 0.0 26-27 0.05547886935742602 0.0 0.0 28-29 0.06277037790154487 0.0 0.0 30-31 0.11343797052141932 0.0 0.0 32-33 0.12233323797805536 0.0 0.0 34-35 0.13033338418119345 0.0 0.0 36-37 0.1389116295272156 0.0 0.0 38-39 0.14845958956452726 0.0 0.0 40-41 0.1592196929659507 0.0 0.0 42-43 0.17102410449645514 0.0 0.0 44-45 0.1831641864970213 0.0 0.0 46-47 0.19646046678335566 0.0 0.0 48-49 0.21178941824950834 0.0 0.0 50-51 0.22924428269271868 0.0 0.0 52-53 0.24725859791936525 0.0 0.0 54-55 0.26792098018760996 0.0 0.0 56-57 0.29123142949745284 0.0 0.0 58-59 0.316574549987114 0.0 0.0 60-61 0.34337224251370935 0.0 0.0 62-63 0.3740674421649104 0.0 0.0 64-65 0.4057883015857446 0.0 0.0 66-67 0.4406047886749259 0.0 0.0 68-69 0.4794866181237438 0.0 0.0 70-71 0.5231424275912175 0.0 0.0 72-73 0.5703041286348915 0.0 0.0 74-75 0.6216803589137854 0.0 0.0 76-77 0.6818213181331798 0.0 0.0 78-79 0.7491791924589017 0.0 1.8648359447874292E-5 80-81 0.8232877729047543 0.0 3.7296718895748584E-5 82-83 0.9063102691666907 0.0 3.7296718895748584E-5 84-85 1.0006709679729346 0.0 3.7296718895748584E-5 86-87 1.104038824392502 0.0 3.7296718895748584E-5 88-89 1.218632993199689 0.0 3.7296718895748584E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2735 0.0 88.44014 9 ATCGGGA 4555 0.0 87.00473 2 CGGGAGA 3855 0.0 85.3046 4 AAGCGGC 905 0.0 81.230606 9 ATCCGGA 680 0.0 79.56841 2 TCGGGAG 4185 0.0 79.49113 3 AGAGCGG 3195 0.0 78.378975 8 GTTCGGA 570 0.0 78.27043 2 ATTCGGA 765 0.0 78.17248 2 CGGAGAG 1500 0.0 73.07625 5 CGGAAAG 1805 0.0 71.506775 5 TCGGAGA 1065 0.0 70.84532 4 TTGGGAG 2405 0.0 69.65554 3 AAGGGGC 2585 0.0 69.35341 9 TCGGGAA 1465 0.0 68.35038 3 GAGAGCG 4565 0.0 66.17758 7 ACCGGAA 490 0.0 65.858055 3 GGATCGG 1740 0.0 65.47809 1 GGAAAGC 1490 0.0 64.94979 6 TCGGAAA 1510 0.0 63.480164 4 >>END_MODULE