FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_30423-1.34200000035748.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_30423-1.34200000035748.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2681201
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA318561.1881242771429668No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC245150.9143290637292766No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC123070.45901071944997784No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT122780.45792911460200114No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT118730.44282394344922293No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT97050.36196465688324003Illumina Single End PCR Primer 1 (97% over 34bp)
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT60950.22732350166958762No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC51500.1920781023131052No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT50810.18950462870929854No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC36880.13755029928752077No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG35040.13068770301070304No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC27040.10085032789410417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC27350.088.440149
ATCGGGA45550.087.004732
CGGGAGA38550.085.30464
AAGCGGC9050.081.2306069
ATCCGGA6800.079.568412
TCGGGAG41850.079.491133
AGAGCGG31950.078.3789758
GTTCGGA5700.078.270432
ATTCGGA7650.078.172482
CGGAGAG15000.073.076255
CGGAAAG18050.071.5067755
TCGGAGA10650.070.845324
TTGGGAG24050.069.655543
AAGGGGC25850.069.353419
TCGGGAA14650.068.350383
GAGAGCG45650.066.177587
ACCGGAA4900.065.8580553
GGATCGG17400.065.478091
GGAAAGC14900.064.949796
TCGGAAA15100.063.4801644
Produced by FastQC (version 0.11.2)