FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_30245-1.3420000003577e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_30245-1.3420000003577e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256156
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG26990.11962825265628796No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC26880.11914069771771102No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC26830.11891908183653967No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG25250.11191601999152542No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA22800.10105684181412988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA9200.074.261282
GAGCGGC5400.070.243249
AGAGCGG5850.066.463858
CGGGAGA8750.065.052584
GATCGGG11650.063.1336631
GAGAGCG9050.055.538547
GGTTCGG2250.054.833511
TCGGGAG11400.054.0940063
GATCCGG2100.049.71171
TTGTGTT287900.049.6606831
GTTCGGA1950.046.2281722
CGGAAAG4750.044.937645
GGACCGG1750.043.3847581
AGCGGCG5450.041.3233710-11
GAGCGTC18550.040.8963289
AGAGCGT19450.040.224618
TCGGAAA4900.039.689824
GGTCCGG1800.039.5433961
CGGCGTG2700.039.51094412-13
GGCGCCG3850.039.4814556-57
Produced by FastQC (version 0.11.2)