FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n02_30230-1.34200000035711.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n02_30230-1.34200000035711.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244879
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA328591.4637314527865422No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC226421.0086066999602206No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT125290.5581147135324442No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT119620.5328572274942214No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC106460.474234914220321No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT59980.2671858928699498No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT58300.2597021933030689No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC53830.23979020695547512No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC31990.14250211258602358No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC30600.13631024211104475No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT29300.1305192841128631No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC27370.12192193877710111No Hit
TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA25920.11546279331759084No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG24670.10989456447318541No Hit
GTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCT23380.10414815230575902No Hit
AACTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAA23030.1025890482293255No Hit
GTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC22820.10165358578346538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC8650.072.325829
TCGGAGA4000.064.023654
CGGGAGA12400.061.9583744
AGAGCGG10300.061.2011578
ATTCGGA2450.058.086892
GTTCGGA2050.055.5367362
AAGCGGC3650.054.5370949
TCGGGAG13550.053.9023253
GGATCGG7200.053.3720931
GGTTCGG3000.052.186051
TTGTGTT293750.051.8591
ATCGGGA15900.049.2277262
TCGGAAA4850.046.9360164
ATCCGGA2250.046.383462
TCGGGAA4100.046.270293
CGGAAAG7050.045.7431955
ACCGGAA1600.044.4628563
TGTGTTT541300.042.1656761
CGGAGAG7600.041.8088235
AAGGGGC11000.041.7940649
Produced by FastQC (version 0.11.2)