##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n02_30230-1.34200000035711.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2244879 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.874678768877967 33.0 33.0 33.0 27.0 33.0 2 31.706126254466277 33.0 33.0 33.0 33.0 33.0 3 31.652849440883006 33.0 33.0 33.0 33.0 33.0 4 35.33846011299495 37.0 37.0 37.0 33.0 37.0 5 35.35875474802874 37.0 37.0 37.0 33.0 37.0 6 35.51976119871049 37.0 37.0 37.0 33.0 37.0 7 35.338925616926346 37.0 37.0 37.0 33.0 37.0 8 35.34206253432813 37.0 37.0 37.0 33.0 37.0 9 35.16317004168153 37.0 37.0 37.0 33.0 37.0 10-11 35.10774322357686 37.0 37.0 37.0 33.0 37.0 12-13 35.078855920519544 37.0 37.0 37.0 33.0 37.0 14-15 36.783666513874465 40.0 37.0 40.0 33.0 40.0 16-17 36.66952071804316 40.0 37.0 40.0 33.0 40.0 18-19 36.7250878555147 40.0 37.0 40.0 33.0 40.0 20-21 36.614769660191044 40.0 37.0 40.0 33.0 40.0 22-23 36.84743810245452 40.0 37.0 40.0 33.0 40.0 24-25 36.53323007609764 40.0 37.0 40.0 33.0 40.0 26-27 36.40198714496416 40.0 37.0 40.0 33.0 40.0 28-29 36.368255482812216 40.0 37.0 40.0 33.0 40.0 30-31 36.34977453127763 40.0 37.0 40.0 33.0 40.0 32-33 36.33921004205572 40.0 37.0 40.0 33.0 40.0 34-35 36.2525764194863 40.0 37.0 40.0 33.0 40.0 36-37 36.07503878828213 40.0 37.0 40.0 33.0 40.0 38-39 36.04728963120061 38.5 37.0 40.0 33.0 40.0 40-41 35.88418529461944 37.0 37.0 40.0 27.0 40.0 42-43 35.77877070434531 37.0 37.0 40.0 27.0 40.0 44-45 35.59804537349229 37.0 37.0 40.0 27.0 40.0 46-47 35.47734644049858 37.0 37.0 40.0 27.0 40.0 48-49 35.343670638818395 37.0 37.0 40.0 27.0 40.0 50-51 34.14829685697982 37.0 35.0 38.5 24.5 38.5 52-53 34.57788214865924 37.0 35.0 38.5 27.0 40.0 54-55 35.332757801199975 37.0 37.0 40.0 27.0 40.0 56-57 35.359440976551525 37.0 37.0 40.0 27.0 40.0 58-59 35.11088170008272 37.0 37.0 40.0 27.0 40.0 60-61 34.89378068929328 37.0 35.0 40.0 27.0 40.0 62-63 34.6594199509194 37.0 33.0 40.0 27.0 40.0 64-65 34.40750926887374 37.0 33.0 37.0 27.0 40.0 66-67 34.127576586533166 37.0 33.0 37.0 27.0 40.0 68-69 33.965799047521045 37.0 33.0 37.0 27.0 40.0 70-71 33.614974348283354 37.0 33.0 37.0 27.0 37.0 72-73 33.45542499172561 37.0 33.0 37.0 27.0 37.0 74-75 33.31776055635961 37.0 33.0 37.0 27.0 37.0 76-77 33.045410688059356 37.0 33.0 37.0 27.0 37.0 78-79 32.87146879631374 37.0 33.0 37.0 22.0 37.0 80-81 32.695533478641835 37.0 33.0 37.0 22.0 37.0 82-83 32.35485966058749 37.0 33.0 37.0 22.0 37.0 84-85 32.212138159785 33.0 33.0 37.0 22.0 37.0 86-87 32.11000125173784 33.0 33.0 37.0 22.0 37.0 88-89 32.039913732544164 33.0 33.0 37.0 22.0 37.0 90-91 31.932666526792758 33.0 33.0 37.0 22.0 37.0 92-93 31.767955422096247 33.0 33.0 37.0 18.5 37.0 94-95 31.573659203903638 33.0 33.0 37.0 15.0 37.0 96-97 31.43016282837516 33.0 33.0 37.0 15.0 37.0 98-99 31.306773772662133 33.0 33.0 37.0 15.0 37.0 100-101 29.93060405482879 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.27998074135700435 2306 2 0.2622828942600961 2306 3 0.2653672537881704 2306 4 0.3392471676245634 2306 5 0.3194082200357542 2306 6 0.21621008315280932 2306 7 0.3681893838469392 2306 8 0.28683509810070973 2306 9 0.42138479653914374 2306 10-11 0.460977609604754 2306 12-13 0.3945447173202936 2306 14-15 0.6468883749047762 2306 16-17 0.6261741762758604 2306 18-19 0.6363263368100505 2306 20-21 0.7340674064353792 2306 22-23 0.558063846122316 2306 24-25 0.506973173190957 2306 26-27 0.45929310541608004 2306 28-29 0.554598958677083 2306 30-31 0.4968021178840658 2306 32-33 0.4080941074821425 2306 34-35 0.4020880441205392 2306 36-37 0.4546330903750615 2306 38-39 0.17636071884978577 2306 40-41 0.30266837790616563 2306 42-43 0.1659078776066707 2306 44-45 0.29339720908161127 2306 46-47 0.3057004508615577 2306 48-49 0.3118459111046761 2306 50-51 0.2513268886747042 2306 52-53 0.3340201745400151 2306 54-55 0.5744742071430338 2306 56-57 0.469016933045431 2306 58-59 0.516885837709431 2306 60-61 0.5203313691953113 2306 62-63 0.5458778621059253 2306 64-65 0.4599397332182775 2306 66-67 0.4198931978543001 2306 68-69 0.2914571055858133 2306 70-71 0.4452194152149431 2306 72-73 0.4152959765708104 2306 74-75 0.368818005224945 2306 76-77 0.419328970075604 2306 78-79 0.3729144710981487 2306 80-81 0.3245824278765994 2306 82-83 0.3531729155650467 2306 84-85 0.39991250958052404 2306 86-87 0.5021745495344163 2306 88-89 0.5929806139612595 2306 90-91 0.39749514111738904 2306 92-93 0.4743230603712938 2306 94-95 0.38314637961331854 2306 96-97 0.33984140700323806 2306 98-99 0.15272103921868663 2306 100-101 -0.03933850263163663 2307 1 0.050205596979374434 2307 2 0.06129390137256152 2307 3 0.10380097870658389 2307 4 0.25206281276138753 2307 5 0.10384104142174522 2307 6 0.07955194622361006 2307 7 0.22195217233717557 2307 8 0.07239521480970268 2307 9 0.18640210880744945 2307 10-11 0.16517707824940686 2307 12-13 0.17888441289183987 2307 14-15 0.2774722949633599 2307 16-17 0.38689628460515024 2307 18-19 0.48972999562708 2307 20-21 0.5851946202121283 2307 22-23 0.358334905848416 2307 24-25 0.3648228965045419 2307 26-27 0.2995591745118489 2307 28-29 0.1875237453113172 2307 30-31 0.25608672896554907 2307 32-33 0.1550779564323932 2307 34-35 0.21056771219468828 2307 36-37 0.22874998528967438 2307 38-39 0.053930281876070296 2307 40-41 0.050187496423845346 2307 42-43 0.05788405305505506 2307 44-45 0.21205743685658263 2307 46-47 0.2274287746458299 2307 48-49 0.09703368764839126 2307 50-51 0.008056655827552106 2307 52-53 0.0732228805706896 2307 54-55 0.2181870730206512 2307 56-57 0.13058744853188387 2307 58-59 0.16650999105551278 2307 60-61 0.2156860056702854 2307 62-63 0.2545106740325451 2307 64-65 0.21641670700442006 2307 66-67 0.161788560971587 2307 68-69 0.16018803395721903 2307 70-71 0.08465110310809365 2307 72-73 0.06363724435400542 2307 74-75 0.11156210168437042 2307 76-77 0.014642507241937608 2307 78-79 0.00403790112901703 2307 80-81 -0.01829409968848239 2307 82-83 -0.14422445440538922 2307 84-85 -0.06865532629623772 2307 86-87 0.11473952150697642 2307 88-89 0.18203449809057304 2307 90-91 0.1317930034640611 2307 92-93 0.030849124998329813 2307 94-95 -0.09817153821741798 2307 96-97 -0.002460219518212625 2307 98-99 -0.08845834784709794 2307 100-101 -0.1696596806813453 2201 1 -0.1516450651351562 2201 2 -0.07329861290994444 2201 3 -0.20209241767528496 2201 4 -0.05678644656027387 2201 5 -0.19905915415461095 2201 6 -0.126603062080207 2201 7 0.07293798456535683 2201 8 -0.1339427798938786 2201 9 0.025235217187855596 2201 10-11 -0.07767210331074637 2201 12-13 -0.10560188904867829 2201 14-15 0.12900459777159767 2201 16-17 0.02365578369762744 2201 18-19 0.1257357944136288 2201 20-21 0.2703821025846409 2201 22-23 0.17902242864457207 2201 24-25 0.266033383939579 2201 26-27 0.04215100251820303 2201 28-29 0.113851816165635 2201 30-31 0.08852333636684051 2201 32-33 -0.06583368613129181 2201 34-35 -0.12893578059293276 2201 36-37 -0.07328418780620183 2201 38-39 -0.06591408402514531 2201 40-41 0.02776996527758513 2201 42-43 -0.14688914563978983 2201 44-45 0.044180838194201044 2201 46-47 0.011001378897162795 2201 48-49 -0.08367355941533816 2201 50-51 -0.1316729007652384 2201 52-53 0.019201931867975475 2201 54-55 -0.06788670536769104 2201 56-57 0.004083982281919418 2201 58-59 -5.163225739153177E-4 2201 60-61 -0.022122409729199433 2201 62-63 -0.018636796959633273 2201 64-65 0.03725172979903846 2201 66-67 0.018856896671714196 2201 68-69 -0.05218712334011144 2201 70-71 0.08468921241732374 2201 72-73 0.099648535818325 2201 74-75 0.008172780067098984 2201 76-77 -0.009404621167611538 2201 78-79 0.006891007793733195 2201 80-81 0.10388280279921247 2201 82-83 -0.1391886254831718 2201 84-85 0.0553705719880071 2201 86-87 0.01754103402996776 2201 88-89 0.09897780439870019 2201 90-91 0.12559846639496186 2201 92-93 0.018621282553009877 2201 94-95 0.05189659567308169 2201 96-97 1.4077940855727888E-4 2201 98-99 0.0589074194398691 2201 100-101 -0.13346293419804667 1101 1 0.21102032500039414 1101 2 0.209158641217023 1101 3 0.17908750892986447 1101 4 0.282322848736527 1101 5 0.23683924443596993 1101 6 0.11907854807834184 1101 7 0.2514540910999514 1101 8 0.08863185365807169 1101 9 0.18196411190191242 1101 10-11 0.18845419931700036 1101 12-13 0.2436569378738298 1101 14-15 0.3978176456944169 1101 16-17 0.38510195578243867 1101 18-19 0.41367206342720664 1101 20-21 0.4994463381535539 1101 22-23 0.2133255046635938 1101 24-25 0.3223475198150467 1101 26-27 0.32068992050449907 1101 28-29 0.33349850603057263 1101 30-31 0.2433360122525201 1101 32-33 0.012513837517808213 1101 34-35 0.09935607970086835 1101 36-37 0.2677093277115645 1101 38-39 0.19266396043614264 1101 40-41 0.08213602593329483 1101 42-43 0.14835418314832083 1101 44-45 0.17980202929330602 1101 46-47 0.16574052909378167 1101 48-49 0.2371308258444813 1101 50-51 0.13543540357024852 1101 52-53 0.32288876875016825 1101 54-55 0.3599983886285685 1101 56-57 0.36644958101995684 1101 58-59 0.36781225221096037 1101 60-61 0.4115330998052471 1101 62-63 0.2996771011306336 1101 64-65 0.28048381100552433 1101 66-67 0.14630649673178198 1101 68-69 0.17708005450786146 1101 70-71 0.2751032544870995 1101 72-73 0.2005832364734843 1101 74-75 0.1802102352192776 1101 76-77 0.1904273932542324 1101 78-79 0.2472380945551933 1101 80-81 0.2452424108290714 1101 82-83 0.22183146736684023 1101 84-85 0.3610235455261517 1101 86-87 0.2239602106673999 1101 88-89 0.18326156748043587 1101 90-91 0.2628737721736627 1101 92-93 0.27025164715509575 1101 94-95 0.11017829036483917 1101 96-97 0.20194447691164186 1101 98-99 0.09780378889209373 1101 100-101 0.17121984156310788 2304 1 0.11481176048197383 2304 2 -0.01406718718332911 2304 3 0.013716458148525845 2304 4 0.14892441244464294 2304 5 0.08132487436250102 2304 6 0.022020758506776872 2304 7 0.18371660189890093 2304 8 0.0551695515184889 2304 9 -0.015651460024741937 2304 10-11 0.11106118878713289 2304 12-13 0.14298162998787234 2304 14-15 0.36856229364359905 2304 16-17 0.25038109953678145 2304 18-19 0.21870764582806856 2304 20-21 0.3326682026445624 2304 22-23 0.16218656371295737 2304 24-25 0.2590824440729378 2304 26-27 0.19660615572875173 2304 28-29 0.18573270245491358 2304 30-31 0.09635061943889411 2304 32-33 0.046171757810760994 2304 34-35 0.05057422151913471 2304 36-37 0.1397531482397696 2304 38-39 0.05245394025062211 2304 40-41 0.2693395059261263 2304 42-43 0.1353176120897075 2304 44-45 0.26202041647188423 2304 46-47 0.2851432468201409 2304 48-49 0.24199421787871955 2304 50-51 0.10104269097789853 2304 52-53 0.1282777009379359 2304 54-55 0.28445202377571377 2304 56-57 0.399632115695141 2304 58-59 0.384366986118728 2304 60-61 0.4557297870285666 2304 62-63 0.2929760840193296 2304 64-65 0.21108691800942836 2304 66-67 0.21893766140978954 2304 68-69 0.144042364894851 2304 70-71 0.3648186622903822 2304 72-73 0.41858304472129504 2304 74-75 0.343186961040459 2304 76-77 0.30161801451068726 2304 78-79 0.2432035667913297 2304 80-81 0.3118237168469946 2304 82-83 0.3124748371743209 2304 84-85 0.310087954671701 2304 86-87 0.32057663516961554 2304 88-89 0.48627705095366736 2304 90-91 0.37039455563595425 2304 92-93 0.4783415531552926 2304 94-95 0.6722204304841348 2304 96-97 0.434235621672169 2304 98-99 0.39580575177464183 2304 100-101 0.3579301524696845 2202 1 0.08967252441654239 2202 2 0.1279083156585905 2202 3 0.08494016250474345 2202 4 0.17016089162644477 2202 5 0.16940467507522783 2202 6 0.12237678526908269 2202 7 0.3273127915688363 2202 8 0.07221012016982797 2202 9 0.07333139692200064 2202 10-11 0.1619592161252612 2202 12-13 0.16804245341118218 2202 14-15 0.26351771522237755 2202 16-17 0.20544088381382863 2202 18-19 0.29032901429788893 2202 20-21 0.40486944332798913 2202 22-23 0.20683207660314196 2202 24-25 0.12088290320398443 2202 26-27 0.22618512035774074 2202 28-29 0.14818007780026932 2202 30-31 0.09234144539809819 2202 32-33 0.02412636523274614 2202 34-35 -0.03546851885256075 2202 36-37 -0.08136216076801617 2202 38-39 -0.017954415629326093 2202 40-41 0.05283123756326091 2202 42-43 0.04700114343283701 2202 44-45 0.10595971507675728 2202 46-47 0.1352131508564156 2202 48-49 0.2198484857191474 2202 50-51 0.14849926133763347 2202 52-53 0.17332099710987592 2202 54-55 0.32504605793216257 2202 56-57 0.2477998577351812 2202 58-59 0.4114442537932632 2202 60-61 0.45463212920703455 2202 62-63 0.27494069965914747 2202 64-65 0.32254596039325634 2202 66-67 0.3171742826400319 2202 68-69 0.264519576131363 2202 70-71 0.3601829021215295 2202 72-73 0.3205846406211563 2202 74-75 0.18069904469078324 2202 76-77 0.21738174411147781 2202 78-79 0.43765976915981497 2202 80-81 0.3396928568724249 2202 82-83 0.26671021619577573 2202 84-85 0.24763824363063236 2202 86-87 0.14152541860582346 2202 88-89 0.2941998023460286 2202 90-91 0.38037715231946834 2202 92-93 0.18411813384928521 2202 94-95 0.19903517072617305 2202 96-97 0.28374400538979216 2202 98-99 0.22338517019594306 2202 100-101 0.015883363240959625 1102 1 -0.06218802956631464 1102 2 -0.18528417684092702 1102 3 -0.14048828128314383 1102 4 -0.13779591361949173 1102 5 -0.12992922197794599 1102 6 -0.13154134612220503 1102 7 0.053690312293333875 1102 8 -0.12288729975354329 1102 9 -0.05373350550210887 1102 10-11 -0.04153684953362102 1102 12-13 -0.07112872245715351 1102 14-15 -0.09720850298621997 1102 16-17 -0.08196287439269412 1102 18-19 -0.010142571882731488 1102 20-21 0.03500517057671715 1102 22-23 -0.07888832058539919 1102 24-25 -0.1177121866402544 1102 26-27 -0.25999728200230976 1102 28-29 -0.21164552555264038 1102 30-31 -0.1453739741219735 1102 32-33 -0.3036582269449184 1102 34-35 -0.20781471858986578 1102 36-37 -0.043118671676431575 1102 38-39 -0.1994890259106441 1102 40-41 -0.11617542346620269 1102 42-43 -0.1639215411659123 1102 44-45 0.04979483705080412 1102 46-47 -0.1007314530858352 1102 48-49 -0.026743515920763627 1102 50-51 -0.00245711276289029 1102 52-53 0.159765094706934 1102 54-55 0.17107456254831988 1102 56-57 0.10964761104425236 1102 58-59 0.18909900101616728 1102 60-61 0.2597281119790509 1102 62-63 0.2945397678503099 1102 64-65 0.18649529690836886 1102 66-67 0.18305018183443877 1102 68-69 0.05657613973533415 1102 70-71 0.16244791951773863 1102 72-73 0.3063782322629507 1102 74-75 0.21789987489636076 1102 76-77 0.13119644516728357 1102 78-79 -0.0398035806951853 1102 80-81 -0.07427747987775746 1102 82-83 0.08417037980821362 1102 84-85 0.005750740274493182 1102 86-87 0.06004105291875561 1102 88-89 0.20266640640191724 1102 90-91 0.25989206360043227 1102 92-93 0.16612544438205035 1102 94-95 0.29304581455884104 1102 96-97 0.283242116482711 1102 98-99 0.2675375795412158 1102 100-101 0.12203080605848626 2305 1 0.0710365701550586 2305 2 0.07406244929713068 2305 3 0.09498507708581272 2305 4 0.14005790414035602 2305 5 0.08273407236090691 2305 6 0.1656542988794456 2305 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-1.1219257904315754 1213 100-101 -0.9566821077886978 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 221.0 3 85.0 4 143.0 5 130.0 6 433.0 7 2189.0 8 9833.0 9 18856.0 10 19405.0 11 16182.0 12 10253.0 13 7209.0 14 6025.0 15 5625.0 16 5797.0 17 5742.0 18 5879.0 19 6406.0 20 7099.0 21 7814.0 22 8541.0 23 9342.0 24 10445.0 25 12166.0 26 14746.0 27 17751.0 28 21782.0 29 26324.0 30 33492.0 31 44026.0 32 61553.0 33 88363.0 34 137423.0 35 243380.0 36 480933.0 37 725613.0 38 173516.0 39 157.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.96339721342209 20.885098399554092 27.08503565447005 12.066468732553764 2 36.52424767072431 19.239460250597936 33.23546210687895 11.000829971798808 3 43.337711037614056 15.31707277903627 27.239655220100083 14.105560963249589 4 35.89171934745411 13.705257016224435 34.959925131724276 15.443098504597192 5 34.91983480733321 12.757020643730376 36.783962143793275 15.539182405143134 6 27.27848886010663 13.438296399571477 43.93659221041496 15.346622529906936 7 37.56941428478428 14.063710876294314 31.346318311816056 17.020556527105345 8 33.73329124657934 26.703109158951506 23.18066184987334 16.38293774459582 9 34.84353474603097 27.685143491500185 20.375391776155936 17.095929986312914 10-11 31.111978338434547 26.524875742198127 26.18831378245853 16.174832136908794 12-13 31.6196880436343 22.813068712254893 26.26363906293608 19.303604181174727 14-15 29.25494811956409 23.414021371908436 26.343713879033704 20.98731662949377 16-17 27.67984539064163 26.180518017139438 27.70505634570439 18.434580246514546 18-19 26.995731238547112 26.428784616677437 27.131722641732754 19.4437615030427 20-21 25.793170593837857 27.902548885039717 27.319987348411072 18.984293172711354 22-23 27.469909977957652 25.263802551222764 27.320293116175232 19.945994354644352 24-25 27.47217838761939 25.278976735570634 27.735797476827067 19.51304739998291 26-27 23.781006945126887 26.111251570113264 29.458317624755885 20.649423860003964 28-29 23.36800795850552 27.20112732114715 27.2494742163644 22.181390503982932 30-31 22.80979588410707 27.363816699758054 27.879609261183248 21.946778154951634 32-33 22.34412357970627 24.92339016629632 30.202864492092935 22.529621761904476 34-35 22.325918271933613 26.80525271595583 28.42343949476903 22.44538951734153 36-37 21.100807227672856 29.641961013542513 28.412743989107764 20.844487769676874 38-39 20.4583687195551 27.98202845175054 27.907485696075916 23.652117132618443 40-41 22.66596992627281 27.593956670340187 26.238796604283838 23.50127679910317 42-43 25.09124285285726 27.097703286887096 26.680864342724213 21.130189517531427 44-45 21.74696516217439 29.267141211576845 26.34680070766357 22.63909291858519 46-47 22.29920569702035 29.39865852342871 27.109338895382564 21.192796884168374 48-49 22.140444565140683 27.11297078605113 28.622554672865054 22.124029975943134 50-51 20.693885947590008 27.200473883789915 27.20541017326823 24.900229995351847 52-53 22.641197571620662 28.383247041585324 26.477576802633873 22.49797858416014 54-55 23.257728689718007 27.963967021875252 27.539413861173884 21.238890427232857 56-57 24.759244026578468 27.463121382016002 25.075383158170737 22.70225143323479 58-59 22.808684998845436 26.130210666560664 26.053835273015235 25.00726906157867 60-61 20.575025831866476 26.047876249954232 28.53362965185699 24.8434682663223 62-63 22.23325567252799 26.11704754395098 28.533894074709753 23.115802708811273 64-65 22.866824024437264 28.320938413698833 27.28273878424044 21.529498777623466 66-67 22.109057768051247 27.8373245032462 26.853785804597486 23.199831924105073 68-69 21.258146308004346 24.745730696029845 30.76444230891175 23.23168068705406 70-71 21.08460753148948 29.061518482691582 28.28962129128917 21.564252694529767 72-73 23.493415820024005 29.88928184210156 26.52396237024675 20.09333996762768 74-75 24.778716412312583 28.085825527088932 26.897497834492732 20.23796022610575 76-77 23.336647087018516 27.310383226348872 26.64215545649766 22.710814230134947 78-79 23.660670233063 28.732180483289717 26.42441158255749 21.18273770108979 80-81 22.723442619876273 29.61877867353983 26.29019207531903 21.367586631264864 82-83 22.42929395682499 29.285180054442776 26.993370969971608 21.292155018760624 84-85 22.913508631986275 28.43001646272127 26.882091900037423 21.77438300525503 86-87 20.59180958191289 29.120365015044452 28.000728886737164 22.28709651630549 88-89 19.99028613474136 29.423833805814255 28.4978233126067 22.08805674683769 90-91 21.55023963616032 28.533226629892088 27.109521674434223 22.80701205951337 92-93 22.007194943225134 28.663533156901327 26.039354531218084 23.289917368655452 94-95 22.631156797923737 29.977781404109 25.768325207201297 21.622736590765967 96-97 21.284413868476758 31.290059246332014 24.725013845573805 22.700513039617427 98-99 21.525970307081657 29.165802392697366 26.527498648606706 22.78072865161427 100-101 23.16114777470997 29.817379178524494 26.23204020181292 20.789432844952614 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 191.0 1 164.5 2 132.5 3 154.5 4 167.5 5 168.0 6 218.0 7 294.0 8 402.0 9 521.0 10 610.0 11 724.5 12 828.5 13 833.0 14 760.0 15 703.0 16 644.0 17 584.0 18 586.0 19 573.0 20 583.5 21 627.0 22 715.0 23 842.5 24 966.0 25 1217.5 26 1538.5 27 1931.5 28 2414.5 29 2990.5 30 3851.0 31 4996.5 32 6653.5 33 9448.5 34 14156.5 35 23450.5 36 35697.5 37 48878.5 38 65337.0 39 79824.0 40 89680.0 41 96690.5 42 132663.0 43 171281.5 44 160871.0 45 138365.0 46 135830.0 47 131837.0 48 116361.5 49 105621.0 50 95666.0 51 89933.0 52 88633.0 53 89522.5 54 76925.0 55 48613.0 56 31712.5 57 25864.5 58 22190.0 59 18290.5 60 14275.5 61 11108.5 62 8608.5 63 6647.5 64 5161.5 65 3917.0 66 3060.0 67 2492.5 68 1959.0 69 1434.5 70 1067.0 71 843.0 72 604.5 73 407.0 74 296.5 75 218.5 76 179.0 77 150.5 78 116.5 79 92.5 80 66.0 81 57.5 82 59.5 83 44.5 84 24.5 85 23.0 86 25.0 87 22.0 88 18.5 89 13.0 90 13.0 91 10.5 92 7.5 93 7.0 94 5.0 95 2.5 96 0.5 97 2.5 98 2.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18112334785081957 2 0.17101144426937934 3 0.14330393753961795 4 0.13697842957237338 5 0.13956208775617748 6 0.12281285539220599 7 0.09733264020020678 8 0.08432525761967571 9 0.08414707429665474 10-11 0.06497009415652247 12-13 0.08343434100457085 14-15 0.08826756364151475 16-17 0.08047204325934716 18-19 0.07673019347590672 20-21 0.07494836024569698 22-23 0.09579580903915089 24-25 0.4381527913085739 26-27 0.27560505488269077 28-29 0.12247876166154167 30-31 0.22816374512835658 32-33 0.25718535386539765 34-35 0.24179476933946106 36-37 0.2443784275232652 38-39 0.26261994521753734 40-41 0.2619963035869639 42-43 0.2888574395323757 44-45 0.26558224296276106 46-47 0.28400194398005413 48-49 0.26803226365429944 50-51 0.28311102736494925 52-53 0.2828214794650402 54-55 0.25921218916476124 56-57 0.27393458622936917 58-59 0.2645131430246352 60-61 0.24041384858604853 62-63 0.23275196569614667 64-65 0.2309478595505593 66-67 0.24119340062426528 68-69 0.2459375315996987 70-71 0.23174968450415367 72-73 0.22284051835310498 74-75 0.2324846907116152 76-77 0.2326851469500138 78-79 0.2347565280801326 80-81 0.2425520484623002 82-83 0.2679208990774113 84-85 0.2619740306715863 86-87 0.26050401825666325 88-89 0.2588780954340969 90-91 0.2517284895978803 92-93 0.22769601390542651 94-95 0.22667145979805592 96-97 0.22243960587630782 98-99 0.24604889617658682 100-101 0.252864408282139 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2244879.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.796243351111464 #Duplication Level Percentage of deduplicated Percentage of total 1 87.57534640031757 40.106218753068276 2 5.696689898192021 5.217739937468404 3 1.8222422677335974 2.503555510134271 4 1.063329437401279 1.9478597471051766 5 0.6717973953810731 1.5382898500757232 6 0.4333479377668095 1.190742456820267 7 0.3456481501433829 1.1080570758479518 8 0.2535458525178049 0.928915805005633 9 0.2414322732073594 0.9951022022954635 >10 1.5413650526975629 14.279400944944634 >50 0.19816202852071715 6.2789489238502805 >100 0.14237189673837408 12.80792232499234 >500 0.009483904167679729 2.9120081089338785 >1k 0.004449383497539151 3.3194940322350064 >5k 2.9554564397071636E-4 0.7764960789455175 >10k+ 4.925760732671835E-4 4.089248248277269 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 32859 1.4637314527865422 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 22642 1.0086066999602206 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 12529 0.5581147135324442 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 11962 0.5328572274942214 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10646 0.474234914220321 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 5998 0.2671858928699498 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5830 0.2597021933030689 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5383 0.23979020695547512 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 3199 0.14250211258602358 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 3060 0.13631024211104475 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 2930 0.1305192841128631 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 2737 0.12192193877710111 No Hit TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA 2592 0.11546279331759084 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2467 0.10989456447318541 No Hit GTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCT 2338 0.10414815230575902 No Hit AACTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAA 2303 0.1025890482293255 No Hit GTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC 2282 0.10165358578346538 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002450020691538386 0.0 0.0 2 0.0028954789990908196 0.0 0.0 3 0.0034300289681537402 0.0 0.0 4 0.0035191206296642267 0.0 0.0 5 0.004098216429482391 0.0 0.0 6 0.004543674737034825 0.0 0.0 7 0.0050782247060977455 0.0 0.0 8 0.005657320505915909 0.0 0.0 9 0.0062809621364893166 0.0 0.0 10-11 0.009532807781622084 0.0 0.0 12-13 0.01265101593448912 0.0 0.0 14-15 0.01619240947953097 0.0 0.0 16-17 0.01937743637853087 0.0 0.0 18-19 0.022272915377621688 0.0 0.0 20-21 0.024945665222936293 0.0 0.0 22-23 0.02817523795269144 0.0 0.0 24-25 0.03207299814377523 0.0 0.0 26-27 0.03608212291174714 0.0 0.0 28-29 0.041561260094642075 0.0 0.0 30-31 0.09274441963241671 0.0 0.0 32-33 0.10131949205280107 0.0 0.0 34-35 0.10780091042768897 0.0 0.0 36-37 0.11463869544861884 0.0 0.0 38-39 0.1226123991538074 0.0 0.0 40-41 0.132056115273919 0.0 0.0 42-43 0.14319257296272986 0.0 0.0 44-45 0.15584358889721894 0.0 0.0 46-47 0.17074416928484787 0.0 0.0 48-49 0.1879165870409942 0.0 0.0 50-51 0.20604674015837826 0.0 0.0 52-53 0.2266269139673007 0.0 0.0 54-55 0.24999120219842583 0.0 0.0 56-57 0.278478260966404 0.0 0.0 58-59 0.31026171121027013 0.0 0.0 60-61 0.3457424654068215 0.0 0.0 62-63 0.3848759777253028 0.0 0.0 64-65 0.42819679813477696 0.0 0.0 66-67 0.47659584325034887 0.0 0.0 68-69 0.5311199400947668 0.0 0.0 70-71 0.5906999887299048 0.0 0.0 72-73 0.6538882496562175 0.0 0.0 74-75 0.7237583851958168 0.0 0.0 76-77 0.8052104367317794 0.0 0.0 78-79 0.8933220899656507 0.0 0.0 80-81 0.9937952112341022 0.0 0.0 82-83 1.1129775814197558 0.0 0.0 84-85 1.2397104699184232 0.0 0.0 86-87 1.3833485011887054 0.0 0.0 88-89 1.539927096293386 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 865 0.0 72.32582 9 TCGGAGA 400 0.0 64.02365 4 CGGGAGA 1240 0.0 61.958374 4 AGAGCGG 1030 0.0 61.201157 8 ATTCGGA 245 0.0 58.08689 2 GTTCGGA 205 0.0 55.536736 2 AAGCGGC 365 0.0 54.537094 9 TCGGGAG 1355 0.0 53.902325 3 GGATCGG 720 0.0 53.372093 1 GGTTCGG 300 0.0 52.18605 1 TTGTGTT 29375 0.0 51.859 1 ATCGGGA 1590 0.0 49.227726 2 TCGGAAA 485 0.0 46.936016 4 ATCCGGA 225 0.0 46.38346 2 TCGGGAA 410 0.0 46.27029 3 CGGAAAG 705 0.0 45.743195 5 ACCGGAA 160 0.0 44.462856 3 TGTGTTT 54130 0.0 42.165676 1 CGGAGAG 760 0.0 41.808823 5 AAGGGGC 1100 0.0 41.794064 9 >>END_MODULE