FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_53115004-1.3410000003588d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_53115004-1.3410000003588d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4753638
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT52335011.009462647345043TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC89060.18735124550922894No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG70700.14872819512129448No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT69990.14723460221413578TruSeq Adapter, Index 13 (97% over 38bp)
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA65910.13865170212792813No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC62480.13143617582996434No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG50130.10545607385333086No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT48880.1028265088759388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA653200.087.346951
TCGGAAG653250.087.0726243
CGGAAGA654400.086.680084
ATCGGAA660300.086.258062
GAAGAGC657950.086.0733646
GGAAGAG680900.083.353575
AGAGCAC836200.067.6799558
GAGCACA837150.067.563449
AAGAGCA843200.067.0336157
CGGACGG250.00499485357.0788041
GCGTGCG250.005022612456.998942
ATCGGAC2200.051.8172152
TCGGACG2300.049.5642973
TTGTGTT409900.046.289211
CGTCTTC613250.046.1085752-53
GCCGTCT614900.046.10675450-51
ATGCCGT614850.046.10661748-49
TCGTATG617300.046.04119544-45
AATCTCG599600.045.97402240-41
TCTCGTA605650.045.9624942-43
Produced by FastQC (version 0.11.2)