##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_52370180-2.341000000358c0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5341839 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.682180237929295 33.0 33.0 33.0 33.0 33.0 2 32.71988129930535 33.0 33.0 33.0 33.0 33.0 3 32.757038166069776 33.0 33.0 33.0 33.0 33.0 4 36.67625830729829 37.0 37.0 37.0 37.0 37.0 5 36.69456024414064 37.0 37.0 37.0 37.0 37.0 6 36.59587662600838 37.0 37.0 37.0 37.0 37.0 7 36.49247347215069 37.0 37.0 37.0 37.0 37.0 8 36.449410586878415 37.0 37.0 37.0 37.0 37.0 9 36.52176600605147 37.0 37.0 37.0 37.0 37.0 10-11 36.44758930398314 37.0 37.0 37.0 37.0 37.0 12-13 36.428551197443426 37.0 37.0 37.0 37.0 37.0 14-15 38.36457070308558 40.0 37.0 40.0 37.0 40.0 16-17 38.400112021346956 40.0 37.0 40.0 37.0 40.0 18-19 38.37053587725126 40.0 37.0 40.0 37.0 40.0 20-21 38.40623800155714 40.0 37.0 40.0 37.0 40.0 22-23 38.218094555077386 40.0 37.0 40.0 37.0 40.0 24-25 38.280287088397834 40.0 37.0 40.0 37.0 40.0 26-27 38.236680195715365 40.0 37.0 40.0 37.0 40.0 28-29 38.07689851753301 40.0 37.0 40.0 37.0 40.0 30-31 38.08515962386736 40.0 37.0 40.0 37.0 40.0 32-33 37.96801794288446 40.0 37.0 40.0 35.0 40.0 34-35 37.801384598075686 40.0 37.0 40.0 33.0 40.0 36-37 37.480006604467114 40.0 37.0 40.0 33.0 40.0 38-39 37.69850373626012 40.0 37.0 40.0 33.0 40.0 40-41 37.48602307182976 40.0 37.0 40.0 33.0 40.0 42-43 37.29071682991569 40.0 37.0 40.0 33.0 40.0 44-45 37.39280985443403 40.0 37.0 40.0 33.0 40.0 46-47 37.345512753192295 40.0 37.0 40.0 33.0 40.0 48-49 37.15889537292307 40.0 37.0 40.0 33.0 40.0 50-51 37.11538498258746 40.0 37.0 40.0 33.0 40.0 52-53 37.05345724571632 37.0 37.0 40.0 33.0 40.0 54-55 36.80553242057651 37.0 37.0 40.0 33.0 40.0 56-57 36.69609267520043 37.0 37.0 40.0 33.0 40.0 58-59 36.40041238607154 37.0 37.0 40.0 33.0 40.0 60-61 36.176691117047895 37.0 37.0 40.0 33.0 40.0 62-63 35.99390350401799 37.0 37.0 40.0 33.0 40.0 64-65 35.73311784574563 37.0 37.0 40.0 33.0 40.0 66-67 35.16810128496947 37.0 33.0 38.5 27.0 40.0 68-69 34.99851427570168 37.0 33.0 37.0 27.0 40.0 70-71 34.55453253083816 37.0 33.0 37.0 27.0 40.0 72-73 33.73642326172691 37.0 33.0 37.0 24.5 40.0 74-75 32.83635167589289 37.0 33.0 37.0 22.0 38.5 76-77 30.286019945565563 33.0 30.0 35.0 18.5 37.0 78-79 32.39891206006021 35.0 33.0 37.0 22.0 37.0 80-81 32.60308762581575 37.0 33.0 37.0 18.5 37.0 82-83 32.43334318012954 37.0 33.0 37.0 15.0 37.0 84-85 32.27087141712807 37.0 33.0 37.0 15.0 37.0 86-87 32.15712173279651 37.0 33.0 37.0 15.0 37.0 88-89 31.895243192466115 37.0 33.0 37.0 15.0 37.0 90-91 31.863293614801943 37.0 33.0 37.0 15.0 37.0 92-93 31.712807705361392 37.0 33.0 37.0 15.0 37.0 94-95 31.548489387269065 35.0 33.0 37.0 15.0 37.0 96-97 31.448092501477486 33.0 33.0 37.0 15.0 37.0 98-99 31.33901124687584 33.0 33.0 37.0 15.0 37.0 100-101 29.92463578554127 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.16246087400823228 2306 2 0.13338614501835622 2306 3 0.12051479291863387 2306 4 0.14716727595607892 2306 5 0.15322129362311898 2306 6 0.11472031922640014 2306 7 0.15603215368599166 2306 8 0.12864722099857318 2306 9 0.1583295656769863 2306 10-11 0.17695397309334737 2306 12-13 0.22774634777591984 2306 14-15 0.42007014552289235 2306 16-17 0.3134916218240349 2306 18-19 0.34801957631341907 2306 20-21 0.4153028533092069 2306 22-23 0.3681837804400985 2306 24-25 0.4097262266540156 2306 26-27 0.3334206469464789 2306 28-29 0.4002307325704777 2306 30-31 0.4290796919508111 2306 32-33 0.4590448928126847 2306 34-35 0.4696225573653905 2306 36-37 0.36054207557781126 2306 38-39 0.46725787852991374 2306 40-41 0.5097942687326835 2306 42-43 0.5539544698106766 2306 44-45 0.59485596814595 2306 46-47 0.5527981969662292 2306 48-49 0.512495675928669 2306 50-51 0.4922770585423635 2306 52-53 0.44508798406073424 2306 54-55 0.4314517523223884 2306 56-57 0.3563712080150907 2306 58-59 0.5204761232668673 2306 60-61 0.4634348990888171 2306 62-63 0.5177097501607903 2306 64-65 0.4718512301394924 2306 66-67 0.31496227995774717 2306 68-69 0.4302514825074155 2306 70-71 0.3020113883389115 2306 72-73 0.1151652416659914 2306 74-75 0.21628361448949818 2306 76-77 -0.08189642403044317 2306 78-79 0.327649537595029 2306 80-81 0.43995484001767693 2306 82-83 0.5061587670185332 2306 84-85 0.5706308004260734 2306 86-87 0.4698392297459151 2306 88-89 0.7117921177259099 2306 90-91 0.47798548403841323 2306 92-93 0.4452419435927979 2306 94-95 0.5110378825738771 2306 96-97 0.4818856855845368 2306 98-99 0.4400387962869843 2306 100-101 0.3688684963470834 2307 1 0.15641485410773015 2307 2 0.09671574700840324 2307 3 0.12104837500818633 2307 4 0.14943468889139666 2307 5 0.14717278616043217 2307 6 0.1581058913657003 2307 7 0.15443016363624196 2307 8 0.16767955930703238 2307 9 0.19512931692077018 2307 10-11 0.14697387359086633 2307 12-13 0.18341612389532003 2307 14-15 0.34325111567213895 2307 16-17 0.3500973432170653 2307 18-19 0.3132154718358038 2307 20-21 0.3063239975878176 2307 22-23 0.36848726302716983 2307 24-25 0.31011117690277246 2307 26-27 0.33375895540418554 2307 28-29 0.327626876849088 2307 30-31 0.30106787603040885 2307 32-33 0.38870969380771214 2307 34-35 0.4057531543803137 2307 36-37 0.3833946875181127 2307 38-39 0.4182398437040362 2307 40-41 0.4042892936083078 2307 42-43 0.34110932055694576 2307 44-45 0.41496479899171845 2307 46-47 0.5494437193542865 2307 48-49 0.44039492966001603 2307 50-51 0.49598912321898325 2307 52-53 0.4330338795831281 2307 54-55 0.43547849361592483 2307 56-57 0.300779168214099 2307 58-59 0.4111726406798013 2307 60-61 0.4345499488400506 2307 62-63 0.41715315812098197 2307 64-65 0.38878220028874466 2307 66-67 0.31523839936073017 2307 68-69 0.33097785066661345 2307 70-71 0.2078154928165219 2307 72-73 0.08248986853165974 2307 74-75 0.10825003239995112 2307 76-77 -0.1726115981597971 2307 78-79 0.26607304505771623 2307 80-81 0.34590384499280447 2307 82-83 0.34656486154590027 2307 84-85 0.26638888500318814 2307 86-87 0.29218313521855066 2307 88-89 0.5093934858851092 2307 90-91 0.35963473776975263 2307 92-93 0.29590425702563294 2307 94-95 0.3876100218773608 2307 96-97 0.2908657850870249 2307 98-99 0.32143867190887576 2307 100-101 0.3036701132625055 2201 1 0.10277320628439668 2201 2 0.09649806255922044 2201 3 0.054063890259449465 2201 4 0.13287580242594998 2201 5 0.12084528728011179 2201 6 0.1271085846569946 2201 7 0.1749323732517425 2201 8 0.19382275161579798 2201 9 0.14110054640399738 2201 10-11 0.1740388718491488 2201 12-13 0.20850943023505408 2201 14-15 0.29925485520818285 2201 16-17 0.2691008557835346 2201 18-19 0.2806148739469876 2201 20-21 0.38990757397278486 2201 22-23 0.34933503684410283 2201 24-25 0.33226468115511665 2201 26-27 0.39488246690255835 2201 28-29 0.3618569523801938 2201 30-31 0.2996683416214623 2201 32-33 0.3720114395313914 2201 34-35 0.3643682880314074 2201 36-37 0.4018312009742502 2201 38-39 0.449318167624817 2201 40-41 0.39437745975488525 2201 42-43 0.3102900089665681 2201 44-45 0.305333066218644 2201 46-47 0.416063444587607 2201 48-49 0.35660820337438537 2201 50-51 0.3245023269005003 2201 52-53 0.28389876107952716 2201 54-55 0.4640824244375352 2201 56-57 0.43034632389701954 2201 58-59 0.5231523857699187 2201 60-61 0.3555106187765418 2201 62-63 0.4152211858768169 2201 64-65 0.43442365634100355 2201 66-67 0.4307452758103949 2201 68-69 0.41478527123636866 2201 70-71 0.11296427318887936 2201 72-73 0.15276410724296596 2201 74-75 0.2862128045358503 2201 76-77 0.09580149009495287 2201 78-79 0.3265796120034281 2201 80-81 0.5013610976414924 2201 82-83 0.552314140280302 2201 84-85 0.463327830189435 2201 86-87 0.47870026292473966 2201 88-89 0.7450794134077832 2201 90-91 0.35736179533385837 2201 92-93 0.4656260311751339 2201 94-95 0.5014584729617759 2201 96-97 0.5081103745330573 2201 98-99 0.5170090634253128 2201 100-101 0.434827846188508 1101 1 0.10310656561820508 1101 2 0.09528920624284609 1101 3 0.09873984960797344 1101 4 0.09770185645493967 1101 5 0.08543047729659037 1101 6 0.08181895868217737 1101 7 0.12983714234812282 1101 8 0.13276400104392394 1101 9 0.1534922640896852 1101 10-11 0.13434399576908618 1101 12-13 0.1562035473223986 1101 14-15 0.29939979404896633 1101 16-17 0.2508901479011243 1101 18-19 0.24281407744719985 1101 20-21 0.2506322183885672 1101 22-23 0.22623593463511327 1101 24-25 0.258019594000956 1101 26-27 0.2843004655405821 1101 28-29 0.28871514300131906 1101 30-31 0.33033734501203327 1101 32-33 0.3621460832888772 1101 34-35 0.22575010838580312 1101 36-37 0.28033430913790625 1101 38-39 0.3377054408881861 1101 40-41 0.3316389399344999 1101 42-43 0.3657869528038731 1101 44-45 0.3078194035200994 1101 46-47 0.37146202916577664 1101 48-49 0.33747923601937657 1101 50-51 0.3527444621704845 1101 52-53 0.3300361133124383 1101 54-55 0.28408072057635536 1101 56-57 0.30686100393346294 1101 58-59 0.3931065087543928 1101 60-61 0.33681159976908503 1101 62-63 0.41858985220159894 1101 64-65 0.3596134183481112 1101 66-67 0.3788523026334829 1101 68-69 0.30278209475230966 1101 70-71 0.04097198924594636 1101 72-73 -0.016020132483667737 1101 74-75 0.009923637165229593 1101 76-77 -0.19914982539098602 1101 78-79 0.16727113623448986 1101 80-81 0.2802059738045273 1101 82-83 0.347965172914229 1101 84-85 0.3207835782038515 1101 86-87 0.2713950687481841 1101 88-89 0.4311977299708083 1101 90-91 0.26278480376630853 1101 92-93 0.31958009552023725 1101 94-95 0.3166070435716115 1101 96-97 0.20978817991560206 1101 98-99 0.1943276284865334 1101 100-101 0.221022974805134 2304 1 0.09898730381758725 2304 2 0.10210754883117801 2304 3 0.09669893503301807 2304 4 0.10166077682262653 2304 5 0.09856314804252975 2304 6 0.10879050986764582 2304 7 0.0659212351418006 2304 8 0.1040865624197167 2304 9 0.07769655007906806 2304 10-11 0.07220613947116306 2304 12-13 0.1367171016754014 2304 14-15 0.22957512819186832 2304 16-17 0.21500245371311166 2304 18-19 0.19492057284721653 2304 20-21 0.22251125885512835 2304 22-23 0.2654706088629766 2304 24-25 0.2583967205881592 2304 26-27 0.2639206469464739 2304 28-29 0.21549958005748238 2304 30-31 0.31794212695947266 2304 32-33 0.2523241389132096 2304 34-35 0.3276047930673016 2304 36-37 0.28862244819480054 2304 38-39 0.31025679534099737 2304 40-41 0.23882286491986093 2304 42-43 0.38245641955071363 2304 44-45 0.3177101709189145 2304 46-47 0.43305426282411474 2304 48-49 0.336753474888809 2304 50-51 0.3466559580224384 2304 52-53 0.3014807483588271 2304 54-55 0.4135953831716108 2304 56-57 0.3149102028157884 2304 58-59 0.3334050660744978 2304 60-61 0.2987210775983442 2304 62-63 0.2475591869025493 2304 64-65 0.19304382112736107 2304 66-67 0.2612551742385065 2304 68-69 0.33374714975177255 2304 70-71 0.2551181907652591 2304 72-73 -0.003754602354810288 2304 74-75 0.11279877913420933 2304 76-77 0.09869499711340524 2304 78-79 0.20395911298498248 2304 80-81 0.2809171450436736 2304 82-83 0.4619692089596157 2304 84-85 0.35856039314704446 2304 86-87 0.3545517514096943 2304 88-89 0.5823967537744359 2304 90-91 0.5507289849049641 2304 92-93 0.41967976854947153 2304 94-95 0.44018779591876367 2304 96-97 0.4198362921703236 2304 98-99 0.43278294706688314 2304 100-101 0.5743271185307925 2202 1 0.09487218117047291 2202 2 0.09725715149152592 2202 3 0.044219167527110415 2202 4 0.07322422939103035 2202 5 0.053393197987290364 2202 6 0.08453124008670443 2202 7 0.09334516266490311 2202 8 0.09295072903783108 2202 9 0.07880059303134601 2202 10-11 0.1316127745132718 2202 12-13 0.11441143965313927 2202 14-15 0.14939711879497253 2202 16-17 0.0893612710201026 2202 18-19 0.04001725328146932 2202 20-21 0.17389071089952068 2202 22-23 0.164556509611117 2202 24-25 0.14769320827857513 2202 26-27 0.11831352646203186 2202 28-29 0.1807229282271976 2202 30-31 0.21224614110511908 2202 32-33 0.17202487297555535 2202 34-35 0.18574601737791596 2202 36-37 0.1820440331890083 2202 38-39 0.18053912200453937 2202 40-41 0.18778998808954128 2202 42-43 0.27988381309737065 2202 44-45 0.22716738493441824 2202 46-47 0.28824228759057746 2202 48-49 0.21470172103203566 2202 50-51 0.2420928081769418 2202 52-53 0.12236959295611882 2202 54-55 0.12177789034703324 2202 56-57 0.26160486848701225 2202 58-59 0.3356087187982908 2202 60-61 0.2248808429184166 2202 62-63 0.24039833337231187 2202 64-65 0.24059013700939857 2202 66-67 0.11813402771301895 2202 68-69 0.1422673522088047 2202 70-71 0.06460631943100026 2202 72-73 -0.02011973432064451 2202 74-75 0.11980465228444359 2202 76-77 -0.20679802144117687 2202 78-79 0.19313724379674824 2202 80-81 0.22216861115987996 2202 82-83 0.3065580413973237 2202 84-85 0.3881509246691266 2202 86-87 0.3165081846425508 2202 88-89 0.5637504336582531 2202 90-91 0.3541075345708862 2202 92-93 0.5290090400638761 2202 94-95 0.453880647242908 2202 96-97 0.347190550900887 2202 98-99 0.3213501086695274 2202 100-101 0.2786625295481713 1102 1 0.09041087400822789 1102 2 0.07943614501835583 1102 3 0.07576479291863336 1102 4 0.10331727595607987 1102 5 0.08127129362311791 1102 6 0.02782031922640016 1102 7 0.023232153685995627 1102 8 0.0937472209985728 1102 9 0.07567956567699241 1102 10-11 0.04005397309335024 1102 12-13 0.023571347775920515 1102 14-15 0.14804514552289305 1102 16-17 0.0982416218240374 1102 18-19 0.0959945763134229 1102 20-21 0.09905285330921032 1102 22-23 0.07988378044010602 1102 24-25 0.06840122665401793 1102 26-27 0.06262064694647052 1102 28-29 0.05655573257048019 1102 30-31 0.07550469195080467 1102 32-33 0.12866989281268104 1102 34-35 0.0336725573653851 1102 36-37 -0.021182924422184612 1102 38-39 0.12173287852991166 1102 40-41 0.17059426873268535 1102 42-43 0.06307946981067403 1102 44-45 0.043830968145947224 1102 46-47 0.08454819696623161 1102 48-49 0.0539456759286665 1102 50-51 -0.03359794145762862 1102 52-53 -0.03558701593925662 1102 54-55 -0.031173247677614313 1102 56-57 0.007871208015089337 1102 58-59 0.08467612326686691 1102 60-61 -0.0219401009111877 1102 62-63 0.05863475016077757 1102 64-65 0.046676230139489405 1102 66-67 0.040062279957744806 1102 68-69 -0.10054851749259086 1102 70-71 -0.26326361166108825 1102 72-73 -0.2197847583340078 1102 74-75 -0.2219663855105054 1102 76-77 -0.18784642403044316 1102 78-79 -0.08302546240496866 1102 80-81 -0.05649515998231891 1102 82-83 0.03858376701853672 1102 84-85 0.10660580042607393 1102 86-87 0.08996422974591667 1102 88-89 0.2598671177259142 1102 90-91 0.05718548403841339 1102 92-93 0.06176694359279722 1102 94-95 0.17113788257387696 1102 96-97 0.026685685584535435 1102 98-99 0.06471379628698415 1102 100-101 0.11659349634708605 2305 1 0.08049672396074925 2305 2 0.06320594558815884 2305 3 0.053595752082927106 2305 4 0.041948614987418864 2305 5 0.06050073331922334 2305 6 0.044289644962390184 2305 7 0.04585551550935918 2305 8 0.06811683163484616 2305 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-0.7773782197290124 1213 90-91 -0.8547571743850781 1213 92-93 -0.9694177034963332 1213 94-95 -0.9568750489047453 1213 96-97 -0.9655872665020162 1213 98-99 -1.1260591557995667 1213 100-101 -0.949491820499901 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 5.0 9 24.0 10 42.0 11 17.0 12 34.0 13 81.0 14 273.0 15 653.0 16 1489.0 17 2705.0 18 4050.0 19 5957.0 20 8251.0 21 11511.0 22 15469.0 23 21168.0 24 27596.0 25 37798.0 26 52378.0 27 79674.0 28 142884.0 29 208504.0 30 138684.0 31 93141.0 32 118104.0 33 166838.0 34 256918.0 35 458498.0 36 978219.0 37 1823643.0 38 686776.0 39 452.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.621275137128656 24.422270759062343 23.124339483527685 12.832114620281315 2 26.950793537581347 29.47009447495516 32.212820341459185 11.366291646004306 3 34.65643221758544 18.19809623222612 32.40130532095006 14.744166229238381 4 25.551672736185548 18.499983428893383 33.72982280525055 22.21852102967052 5 36.54033751298008 17.483791630560187 31.889635760269076 14.086235096190656 6 30.13263784251079 18.09092336927414 37.52612162216046 14.25031716605461 7 30.039973125360014 26.220127562811236 28.504471961809408 15.235427350019348 8 25.460651285072426 33.705920376858984 26.346245178860688 14.487183159207905 9 36.17128857683655 23.370285027309883 24.565734759134447 15.892691636719114 10-11 26.20740709817658 30.835493260381757 28.166640567515667 14.790459073925993 12-13 22.522215663931465 29.11278306965073 27.579228801167538 20.78577246525026 14-15 21.436138378562138 29.97710151878407 28.15917888951726 20.427581213136527 16-17 24.531274716737414 26.661371922254308 28.8581791632931 19.949174197715177 18-19 19.163531510403065 27.001141367233267 33.28394023107023 20.55138689129343 20-21 23.452691105067 27.136347613621453 32.95072165222501 16.460239629086537 22-23 19.000998445562438 35.75105299174707 28.745572539311866 16.50237602337862 24-25 18.99592069322943 27.147224017796116 32.971641414127234 20.88521387484722 26-27 19.08300680720628 27.235498860972783 28.37951499474245 25.301979337078485 28-29 22.845821448381354 31.60379786811246 28.361262853485474 17.189117830020713 30-31 18.15090271346628 27.929669913301392 32.33993012518723 21.5794972480451 32-33 17.937333940614835 32.245505714417824 28.514309397943293 21.302850947024048 34-35 22.489109087713054 32.18701462174356 28.76271636041446 16.561159930128934 36-37 18.122016914199452 32.222212654049656 33.180927944671666 16.474842487079222 38-39 18.514282184172174 27.94724432517534 32.524207686742 21.014265803910483 40-41 22.659085964999644 27.791233099930245 32.282086497055346 17.267594438014765 42-43 18.424738899074548 32.10545672080807 32.55359452365047 16.916209856466917 44-45 18.001217759444305 28.01784611957349 32.8261145283569 21.1548215926253 46-47 22.40324571118764 27.920329446088154 28.461059777908076 21.215365064816126 48-49 18.521805559832938 32.287509186493466 32.03335304414019 17.157332209533397 50-51 22.792486902852005 27.88357932579373 31.704042225390484 17.61989154596379 52-53 18.300809109547057 27.757696397042427 27.78177096530314 26.15972352810739 54-55 22.63880775855613 27.730172994832618 31.924698569609827 17.706320677001422 56-57 18.483548225618502 27.635644367443575 31.833142615123478 22.047664791814448 58-59 18.540564037464684 27.711024867384904 32.21238750806448 21.536023587085936 60-61 22.449816562053382 28.08939486119124 32.02577258450123 17.435015992254147 62-63 18.226004841250653 28.231000346980323 31.93265418042958 21.610340631339444 64-65 22.537714748547746 27.995105423801547 31.960195198889203 17.506984628761504 66-67 18.594030420098715 35.95703196871641 27.661622867818238 17.787314743366643 68-69 18.473574866911683 36.21646180510663 27.61286609175672 17.697097236224963 70-71 18.078171107189615 36.69566026736212 27.717382246035854 17.50878637941241 72-73 17.985129414690384 36.55554316511769 27.63487732857915 17.824450091612775 74-75 18.50811348295986 35.475241026294036 27.624623491508256 18.392021999237855 76-77 18.958221377231645 34.54513260050252 27.99484503309125 18.501800989174587 78-79 18.943062114371944 34.019215741820894 28.420317881982594 18.617404261824568 80-81 18.49428872300473 34.042026768237925 28.271099625014756 19.19258488374259 82-83 18.137502900481497 33.73715586296006 28.34664551768318 19.778695718875273 84-85 18.17018997263371 33.07020481936508 28.983665412713854 19.77593979528735 86-87 18.11561570979963 32.92631914408776 29.556218064460804 19.40184708165181 88-89 18.37233524149414 33.255811667383654 29.057461557869978 19.314391533252227 90-91 18.774128910914058 32.604175804854094 28.890747240567777 19.730948043664064 92-93 18.389523630264943 33.09994765373555 28.766557198360506 19.743971517639007 94-95 18.137657917185486 33.308709061797465 28.616212376945356 19.937420644071697 96-97 18.593442382583454 33.388019313557685 28.337736893645943 19.68080141021292 98-99 18.858523060587093 32.634432892289354 28.209710396050035 20.297333651073522 100-101 18.768305768853057 32.68859143012441 27.925800866562778 20.617301934459753 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 477.0 1 334.0 2 268.0 3 616.0 4 721.0 5 473.0 6 416.5 7 399.0 8 364.5 9 357.5 10 346.5 11 363.0 12 380.0 13 390.0 14 424.0 15 510.0 16 632.5 17 829.0 18 1163.0 19 1713.5 20 2710.5 21 4238.0 22 6598.0 23 10053.5 24 14594.0 25 20668.5 26 28813.0 27 39302.5 28 50856.5 29 62971.5 30 75986.0 31 89590.5 32 106005.5 33 127729.0 34 155909.5 35 195706.5 36 253793.5 37 326823.0 38 393435.5 39 414784.0 40 411223.0 41 381448.5 42 327890.5 43 302473.5 44 275059.0 45 244472.5 46 237233.0 47 209379.5 48 148594.5 49 100586.0 50 74057.5 51 57123.5 52 45066.0 53 33826.5 54 25385.0 55 19329.5 56 14514.5 57 10941.0 58 8480.0 59 6466.5 60 4767.0 61 3549.0 62 2623.0 63 1930.5 64 1340.5 65 848.5 66 564.5 67 368.0 68 241.0 69 168.5 70 117.0 71 80.5 72 53.0 73 34.5 74 25.0 75 21.5 76 17.0 77 12.0 78 6.5 79 3.0 80 1.5 81 0.5 82 0.0 83 1.0 84 1.5 85 0.5 86 1.0 87 2.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1664408081187022 2 0.0 3 2.0592159366839772E-4 4 0.02280113646255531 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.709644188078301E-4 12-13 0.0 14-15 0.0 16-17 0.0011980892722524958 18-19 0.0 20-21 0.0 22-23 6.645651432025562E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0010296079683419885 38-39 8.236863746735909E-4 40-41 0.005485002449530958 42-43 0.0033415458608917264 44-45 0.0011512889100551327 46-47 0.004998278682678381 48-49 0.008190063384538544 50-51 0.005868765419549335 52-53 0.0019188148500918877 54-55 0.011962172577646013 56-57 0.019890153933879325 58-59 0.024139626821399897 60-61 0.012345935547664392 62-63 7.020054329604468E-4 64-65 0.01544411952512983 66-67 0.03585843751561962 68-69 0.02093848204710026 70-71 0.03818909555304831 72-73 0.031814886221767445 74-75 0.03096311962977544 76-77 0.039958149244108634 78-79 0.017924538721590076 80-81 0.01301986076330642 82-83 0.0010015277510235707 84-85 0.011157206347851367 86-87 0.022426733564976407 88-89 0.02211785117447381 90-91 0.02589932044002075 92-93 0.04481602684019492 94-95 0.029568468836294017 96-97 0.030935039412457024 98-99 0.038310776494761445 100-101 0.018121100242819 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5341839.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.89855345530242 #Duplication Level Percentage of deduplicated Percentage of total 1 93.37015820503645 49.391463049391646 2 3.4478892938620165 3.6477671223864965 3 0.958067801726769 1.5204120247034272 4 0.49903589975501955 1.0559310887722337 5 0.2859800847486254 0.7563966400113539 6 0.21892617803165185 0.6948526878823436 7 0.13175437686955974 0.48787311634631264 8 0.10802366194814154 0.4571436364801027 9 0.06989635521075684 0.33276744742023234 >10 0.696038118735829 7.384894911112179 >50 0.09851935391711085 3.630854555887422 >100 0.09088412915573765 9.918678764571167 >500 0.016864733987592976 6.221752725490772 >1k 0.007680266432440133 6.120394721193046 >5k 2.4634869122014316E-4 0.8629793270149175 >10k+ 3.519189094176475E-5 7.515838181336232 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 403730 7.557884091976565 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8460 0.1583724256758768 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 7692 0.14399535440884684 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6760 0.12654817938166987 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6529 0.12222382591463352 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6171 0.11552201404797112 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5517 0.10327903929714094 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0013104101415261672 0.0 0.0 2 0.001366570576163003 0.0 1.8720144878945247E-5 3 0.0014976115903156198 0.0 1.8720144878945247E-5 4 0.0015724921698314007 0.0 1.8720144878945247E-5 5 0.0018720144878945247 0.0 1.8720144878945247E-5 6 0.0019468950674103058 0.0 1.8720144878945247E-5 7 0.002265137530352375 0.0 5.616043463683574E-5 8 0.002564659848415499 0.0 7.488057951578099E-5 9 0.0029016224562365134 0.0 9.360072439472624E-5 10-11 0.005924925854186171 0.0 9.360072439472624E-5 12-13 0.00820878352941749 0.0 1.3104101415261672E-4 14-15 0.010267999466101468 0.0 1.3104101415261672E-4 16-17 0.011335047724201347 0.0 1.4976115903156197E-4 18-19 0.012551857141332789 0.0 1.4976115903156197E-4 20-21 0.013637625544311614 0.0 1.4976115903156197E-4 22-23 0.015425399380250882 0.0 1.8720144878945247E-4 24-25 0.017493975389374333 0.0 2.1528166610787036E-4 26-27 0.019440870456784637 0.0 2.2464173854734297E-4 28-29 0.021930649725684356 0.0 2.2464173854734297E-4 30-31 0.03923742366626924 0.0 2.3400181098681558E-4 32-33 0.042045445398111024 0.0 2.4336188342628822E-4 34-35 0.044319943000902876 0.0 2.4336188342628822E-4 36-37 0.046697401400528915 0.0 2.4336188342628822E-4 38-39 0.0494399026252944 0.0 2.4336188342628822E-4 40-41 0.05278144848618613 0.0 2.4336188342628822E-4 42-43 0.056085554057319964 0.0 2.4336188342628822E-4 44-45 0.05963302151188009 0.0 2.4336188342628822E-4 46-47 0.06354553179157965 0.0 2.527219558657608E-4 48-49 0.06816940757667911 0.0 2.6208202830523344E-4 50-51 0.07329872727351011 0.0 2.6208202830523344E-4 52-53 0.07882117001279895 0.0 2.6208202830523344E-4 54-55 0.0852702599235956 0.0 2.6208202830523344E-4 56-57 0.0921405530941685 0.0 2.6208202830523344E-4 58-59 0.10033061647870706 0.0 2.6208202830523344E-4 60-61 0.1096158083386639 0.0 2.6208202830523344E-4 62-63 0.11970596642841538 0.0 2.714421007447061E-4 64-65 0.1305074900235668 0.0 2.808021731841787E-4 66-67 0.14292830615074698 0.0 2.9016224562365136E-4 68-69 0.1569964950272743 0.0 3.088823905025966E-4 70-71 0.17243125447996466 0.0 3.276025353815418E-4 72-73 0.19032771298423634 0.0 3.3696260782101444E-4 74-75 0.21066715039521033 0.0 3.3696260782101444E-4 76-77 0.2333091656262946 0.0 3.463226802604871E-4 78-79 0.25952672852925746 0.0 3.6504282513943236E-4 80-81 0.2892355984521435 0.0 3.7440289757890494E-4 82-83 0.32202393220761616 0.0 3.7440289757890494E-4 84-85 0.3591834197923225 0.0 3.7440289757890494E-4 86-87 0.4004332590330783 0.0 3.7440289757890494E-4 88-89 0.44605425210306787 0.0 3.7440289757890494E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 49980 0.0 88.35383 1 TCGGAAG 50110 0.0 87.96909 3 ATCGGAA 50240 0.0 87.760376 2 CGGAAGA 50220 0.0 87.62507 4 GAAGAGC 51640 0.0 85.144844 6 GGAAGAG 53755 0.0 81.93616 5 AGAGCAC 69390 0.0 63.392117 8 GAGCACA 69630 0.0 63.11224 9 TCGGACG 145 0.0 62.243233 3 AAGAGCA 71275 0.0 61.62232 7 GCCGTCT 47055 0.0 46.425877 50-51 ATGCCGT 46905 0.0 46.36594 48-49 TCGTATG 47120 0.0 46.23437 44-45 TCTCGTA 46735 0.0 46.1681 42-43 TGCCGTC 47030 0.0 46.1526 50-51 CGTATCT 46300 0.0 46.150074 38-39 TATGCCG 46895 0.0 46.13782 48-49 CGTCTTC 47210 0.0 46.08444 52-53 TATCTCG 46470 0.0 46.07366 40-41 CTCGTAT 46740 0.0 46.01074 44-45 >>END_MODULE