FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_52370180-2.341000000358c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_52370180-2.341000000358c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5341839
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4037307.557884091976565TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC84600.1583724256758768No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC76920.14399535440884684No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG67600.12654817938166987No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA65290.12222382591463352No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG61710.11552201404797112No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA55170.10327903929714094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA499800.088.353831
TCGGAAG501100.087.969093
ATCGGAA502400.087.7603762
CGGAAGA502200.087.625074
GAAGAGC516400.085.1448446
GGAAGAG537550.081.936165
AGAGCAC693900.063.3921178
GAGCACA696300.063.112249
TCGGACG1450.062.2432333
AAGAGCA712750.061.622327
GCCGTCT470550.046.42587750-51
ATGCCGT469050.046.3659448-49
TCGTATG471200.046.2343744-45
TCTCGTA467350.046.168142-43
TGCCGTC470300.046.152650-51
CGTATCT463000.046.15007438-39
TATGCCG468950.046.1378248-49
CGTCTTC472100.046.0844452-53
TATCTCG464700.046.0736640-41
CTCGTAT467400.046.0107444-45
Produced by FastQC (version 0.11.2)