##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_52260024-1.34100000035820.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6655324 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66214807874117 33.0 33.0 33.0 33.0 33.0 2 32.70686445919087 33.0 33.0 33.0 33.0 33.0 3 32.7377667864104 33.0 33.0 33.0 33.0 33.0 4 36.66097157704118 37.0 37.0 37.0 37.0 37.0 5 36.66860050690244 37.0 37.0 37.0 37.0 37.0 6 36.55942415425605 37.0 37.0 37.0 37.0 37.0 7 36.49497845634563 37.0 37.0 37.0 37.0 37.0 8 36.44163469727394 37.0 37.0 37.0 37.0 37.0 9 36.46327857216268 37.0 37.0 37.0 37.0 37.0 10-11 36.41266999172392 37.0 37.0 37.0 37.0 37.0 12-13 36.38752944259363 37.0 37.0 37.0 37.0 37.0 14-15 38.277019646226094 40.0 37.0 40.0 37.0 40.0 16-17 38.26703718707008 40.0 37.0 40.0 37.0 40.0 18-19 38.27300774537798 40.0 37.0 40.0 37.0 40.0 20-21 38.22997558045258 40.0 37.0 40.0 37.0 40.0 22-23 38.101470792406204 40.0 37.0 40.0 37.0 40.0 24-25 38.11455745805914 40.0 37.0 40.0 37.0 40.0 26-27 38.03289306425953 40.0 37.0 40.0 37.0 40.0 28-29 37.91349128907924 40.0 37.0 40.0 33.0 40.0 30-31 37.864232755610395 40.0 37.0 40.0 33.0 40.0 32-33 37.74216709810071 40.0 37.0 40.0 33.0 40.0 34-35 37.635820960782674 40.0 37.0 40.0 33.0 40.0 36-37 37.49612408051058 40.0 37.0 40.0 33.0 40.0 38-39 37.44569918759778 40.0 37.0 40.0 33.0 40.0 40-41 37.394071122608004 40.0 37.0 40.0 33.0 40.0 42-43 37.18638348786625 40.0 37.0 40.0 33.0 40.0 44-45 37.108105555792626 40.0 37.0 40.0 33.0 40.0 46-47 36.98463921516067 37.0 37.0 40.0 33.0 40.0 48-49 36.852739776455664 37.0 37.0 40.0 33.0 40.0 50-51 36.72758140700588 37.0 37.0 40.0 33.0 40.0 52-53 36.58095774150139 37.0 37.0 40.0 33.0 40.0 54-55 36.4078067423915 37.0 37.0 40.0 33.0 40.0 56-57 36.21410640563855 37.0 37.0 40.0 33.0 40.0 58-59 35.94866666145781 37.0 37.0 40.0 33.0 40.0 60-61 35.660318866519496 37.0 35.0 40.0 27.0 40.0 62-63 35.40843631354386 37.0 33.0 38.5 27.0 40.0 64-65 35.12523319676097 37.0 33.0 37.0 27.0 40.0 66-67 34.73152441564077 37.0 33.0 37.0 27.0 40.0 68-69 34.43872109006263 37.0 33.0 37.0 27.0 40.0 70-71 34.073127844715 37.0 33.0 37.0 27.0 38.5 72-73 33.5955938433651 37.0 33.0 37.0 27.0 37.0 74-75 33.07502001405191 37.0 33.0 37.0 27.0 37.0 76-77 30.33711499244815 33.0 30.0 35.0 22.0 37.0 78-79 32.7825978119172 35.0 33.0 37.0 27.0 37.0 80-81 33.17590022664561 37.0 33.0 37.0 27.0 37.0 82-83 33.0722805531331 37.0 33.0 37.0 27.0 37.0 84-85 32.94481095135263 37.0 33.0 37.0 27.0 37.0 86-87 32.85033861612147 37.0 33.0 37.0 27.0 37.0 88-89 32.59857228588721 37.0 33.0 37.0 22.0 37.0 90-91 32.59237281310422 35.0 33.0 37.0 24.5 37.0 92-93 32.438043437103886 33.0 33.0 37.0 22.0 37.0 94-95 32.26434701000282 33.0 33.0 37.0 22.0 37.0 96-97 32.15192738926009 33.0 33.0 37.0 22.0 37.0 98-99 32.02377284712209 33.0 33.0 37.0 22.0 37.0 100-101 30.382877527825844 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.18681447171167065 2306 2 0.13013052852633678 2306 3 0.1291553120440767 2306 4 0.17194007994061877 2306 5 0.16814206289539158 2306 6 0.19262114201282543 2306 7 0.19905611112387334 2306 8 0.21417950044924794 2306 9 0.20650270413606364 2306 10-11 0.22701175033379428 2306 12-13 0.24815109917491895 2306 14-15 0.4100562517262958 2306 16-17 0.3756683973354811 2306 18-19 0.3967939657468733 2306 20-21 0.44561808602572484 2306 22-23 0.38975888944424497 2306 24-25 0.43738146779190856 2306 26-27 0.4349934480191777 2306 28-29 0.3909408757838051 2306 30-31 0.4170840464157237 2306 32-33 0.42611440625810815 2306 34-35 0.4796285943826888 2306 36-37 0.44468807027802626 2306 38-39 0.48058719268023964 2306 40-41 0.4632341966407836 2306 42-43 0.5183217077842528 2306 44-45 0.4078090151315408 2306 46-47 0.5800972484346261 2306 48-49 0.49984079992663766 2306 50-51 0.47290350429624795 2306 52-53 0.4632912899514352 2306 54-55 0.519525832634308 2306 56-57 0.45888487262269706 2306 58-59 0.47268342381277506 2306 60-61 0.5199434512711392 2306 62-63 0.46524525156327456 2306 64-65 0.42534034360356543 2306 66-67 0.3803569645982634 2306 68-69 0.3507388292862501 2306 70-71 0.35133862256915194 2306 72-73 0.2888763684123674 2306 74-75 0.29828781623594836 2306 76-77 -0.06310251079390383 2306 78-79 0.3127231681302618 2306 80-81 0.4426962434420503 2306 82-83 0.5355393204899599 2306 84-85 0.5072538448324977 2306 86-87 0.33150018052278796 2306 88-89 0.6547879833678891 2306 90-91 0.44333372868891274 2306 92-93 0.47056089395008627 2306 94-95 0.4349474281766348 2306 96-97 0.3957608483928823 2306 98-99 0.31760285697227886 2306 100-101 0.2994413415744468 2307 1 0.1475619790459035 2307 2 0.13608611602871434 2307 3 0.13644514227019755 2307 4 0.16416652941430954 2307 5 0.15674875457270332 2307 6 0.16540378094656916 2307 7 0.17352708553291052 2307 8 0.19360998545415953 2307 9 0.1733684869758534 2307 10-11 0.17287895622103377 2307 12-13 0.2120846167726924 2307 14-15 0.42598899775258303 2307 16-17 0.38151722209262573 2307 18-19 0.3337333205660755 2307 20-21 0.36097256367818886 2307 22-23 0.3957585173331424 2307 24-25 0.4186093234208528 2307 26-27 0.4120913208805703 2307 28-29 0.3997958490615474 2307 30-31 0.4153614438999682 2307 32-33 0.3856955673010489 2307 34-35 0.37353737455568137 2307 36-37 0.36071562178295125 2307 38-39 0.4309796387766198 2307 40-41 0.34587816989287035 2307 42-43 0.3799259997230209 2307 44-45 0.44215454401876997 2307 46-47 0.46948175366722467 2307 48-49 0.41400473923987846 2307 50-51 0.46183995590881466 2307 52-53 0.3599138829834416 2307 54-55 0.35123796359295767 2307 56-57 0.37312764221682926 2307 58-59 0.48154366584908814 2307 60-61 0.40535175311325844 2307 62-63 0.37413490212070144 2307 64-65 0.4309286664579659 2307 66-67 0.2990792447947044 2307 68-69 0.24475823327944823 2307 70-71 0.18562039947768483 2307 72-73 0.22677141972886972 2307 74-75 0.1760583084691163 2307 76-77 -0.17637555129633853 2307 78-79 0.12735157063221436 2307 80-81 0.2444389517014045 2307 82-83 0.3252257416764479 2307 84-85 0.22454006254033487 2307 86-87 0.17691452253408357 2307 88-89 0.5203058367775171 2307 90-91 0.3215405223951393 2307 92-93 0.30484878422733885 2307 94-95 0.2406615134827419 2307 96-97 0.22430653588985905 2307 98-99 0.21301717485183502 2307 100-101 0.266240943175859 2201 1 0.16970199741479775 2201 2 0.14947593864615527 2201 3 0.11323177107041715 2201 4 0.17019355792842106 2201 5 0.1591337479488999 2201 6 0.1038921141599829 2201 7 0.14000004643764186 2201 8 0.21297774000511538 2201 9 0.17527859790873634 2201 10-11 0.1846170598232817 2201 12-13 0.21124637730835616 2201 14-15 0.36207773558954415 2201 16-17 0.369691506488806 2201 18-19 0.3663922813269025 2201 20-21 0.37550938399739664 2201 22-23 0.3657740504754585 2201 24-25 0.41157771961331946 2201 26-27 0.43172699039286755 2201 28-29 0.37780269718805215 2201 30-31 0.39672505910128564 2201 32-33 0.38161610702484694 2201 34-35 0.5099249243400337 2201 36-37 0.4469112293319739 2201 38-39 0.5196277475001452 2201 40-41 0.5230474134246066 2201 42-43 0.43845039352839166 2201 44-45 0.39749718312928195 2201 46-47 0.5685984384629421 2201 48-49 0.4272292841441967 2201 50-51 0.4035525800787241 2201 52-53 0.34575798441019856 2201 54-55 0.39673356394679615 2201 56-57 0.5889902818352297 2201 58-59 0.5707481319449315 2201 60-61 0.4827637284464501 2201 62-63 0.5357765055473109 2201 64-65 0.4412433924694241 2201 66-67 0.4218054594060163 2201 68-69 0.3824227174055963 2201 70-71 0.32151634858653466 2201 72-73 0.38351267651668053 2201 74-75 0.3904486855393259 2201 76-77 0.15866628660876003 2201 78-79 0.4560815352064864 2201 80-81 0.4832895838198752 2201 82-83 0.5694763627905175 2201 84-85 0.5880134763187002 2201 86-87 0.5328592296264176 2201 88-89 0.7413250987204663 2201 90-91 0.4916036707556941 2201 92-93 0.6531401385734412 2201 94-95 0.658823862330884 2201 96-97 0.5863576301933904 2201 98-99 0.5143683889340949 2201 100-101 0.5186589497700389 1101 1 0.12174791270078344 1101 2 0.12384309797005244 1101 3 0.09076539813531781 1101 4 0.10560595305530285 1101 5 0.11800404363066974 1101 6 0.104804817554637 1101 7 0.10932476342755137 1101 8 0.15539583922222278 1101 9 0.1816373768256696 1101 10-11 0.11637953312743576 1101 12-13 0.15710058676327066 1101 14-15 0.2868839185701546 1101 16-17 0.2965915322622976 1101 18-19 0.2779811909612562 1101 20-21 0.2959295094237362 1101 22-23 0.24225509087129637 1101 24-25 0.28114305332061207 1101 26-27 0.2628950821786802 1101 28-29 0.24493365975633452 1101 30-31 0.3244271231139635 1101 32-33 0.41832883958515765 1101 34-35 0.35548314078632615 1101 36-37 0.399078126164909 1101 38-39 0.36458413364373143 1101 40-41 0.42738236112749206 1101 42-43 0.4628266277797266 1101 44-45 0.4219931749489021 1101 46-47 0.4825427376424898 1101 48-49 0.4422289152020582 1101 50-51 0.4741074305837216 1101 52-53 0.3521885234469053 1101 54-55 0.38492283441005526 1101 56-57 0.44498134259458055 1101 58-59 0.5205776841750804 1101 60-61 0.4458979635839455 1101 62-63 0.5841555906197655 1101 64-65 0.5318011490494428 1101 66-67 0.48993326515850555 1101 68-69 0.4910251016460663 1101 70-71 0.3507106873705439 1101 72-73 0.3852703798011774 1101 74-75 0.3467399183201252 1101 76-77 0.08452937515452419 1101 78-79 0.45969083842341973 1101 80-81 0.5308245432729635 1101 82-83 0.5924505994387417 1101 84-85 0.5698074933012265 1101 86-87 0.5721812573603771 1101 88-89 0.7130914083234146 1101 90-91 0.6565855191523013 1101 92-93 0.611619163251298 1101 94-95 0.6728912587918998 1101 96-97 0.7225039753756448 1101 98-99 0.6212772063713814 1101 100-101 0.5160206987585916 2304 1 0.10081468862156129 2304 2 0.07666546228045945 2304 3 0.09091496410120214 2304 4 0.08691036224572457 2304 5 0.08479925274802724 2304 6 0.1089043875253708 2304 7 0.15788418785730585 2304 8 0.11391137764498183 2304 9 0.10147680488690014 2304 10-11 0.10258249882192416 2304 12-13 0.12055217388373762 2304 14-15 0.22222841373233848 2304 16-17 0.262871874407999 2304 18-19 0.15641563469872466 2304 20-21 0.1955475383351768 2304 22-23 0.22324107979064678 2304 24-25 0.16072131881709595 2304 26-27 0.21033768852442591 2304 28-29 0.160074355410039 2304 30-31 0.25255155133864804 2304 32-33 0.17657424830196788 2304 34-35 0.2355586891057797 2304 36-37 0.2611603496441077 2304 38-39 0.26565112739105956 2304 40-41 0.17872937663268118 2304 42-43 0.23257724427067927 2304 44-45 0.27582719328940897 2304 46-47 0.30598090503646347 2304 48-49 0.15594622437183858 2304 50-51 0.1828053716270901 2304 52-53 0.21389811790066204 2304 54-55 0.31141917187202495 2304 56-57 0.09400114637406887 2304 58-59 0.2654737339696638 2304 60-61 0.1746894120173792 2304 62-63 0.15321181140355122 2304 64-65 0.118446507136305 2304 66-67 0.18309190036209344 2304 68-69 0.08918583068022201 2304 70-71 -0.03515991467507007 2304 72-73 -0.0647373122955699 2304 74-75 0.02672828954304407 2304 76-77 -0.0473346515039772 2304 78-79 0.11441109785700121 2304 80-81 0.11430978970744121 2304 82-83 0.24062566691307552 2304 84-85 0.23224830947219743 2304 86-87 0.19351998585135988 2304 88-89 0.4487249187221565 2304 90-91 0.2668800079392639 2304 92-93 0.2801699675750555 2304 94-95 0.26281283039029546 2304 96-97 0.18647389981958185 2304 98-99 0.3105885073086725 2304 100-101 0.2601262674803131 2202 1 0.07510972574792163 2202 2 0.08725030754784768 2202 3 0.058628911335219414 2202 4 0.06867630687160187 2202 5 0.05684615113744229 2202 6 0.08101853792827995 2202 7 0.09553111619929666 2202 8 0.10430620771708732 2202 9 0.09315648750261118 2202 10-11 0.09572823294617905 2202 12-13 0.04125533889630617 2202 14-15 0.023741513393190417 2202 16-17 -0.004512594474171294 2202 18-19 -0.0026545214432189823 2202 20-21 0.12524396472424826 2202 22-23 0.14162129526553002 2202 24-25 0.07482396714769379 2202 26-27 0.06476893923527172 2202 28-29 0.08100654461143364 2202 30-31 0.07076483770860165 2202 32-33 0.13567018909193962 2202 34-35 0.10483045293818805 2202 36-37 0.09299619539115866 2202 38-39 0.199498458256123 2202 40-41 0.1718239653180973 2202 42-43 0.2434825962452507 2202 44-45 0.16972654556492017 2202 46-47 0.33028254727128825 2202 48-49 0.2361010170314728 2202 50-51 0.2030037226811814 2202 52-53 0.08800517434642785 2202 54-55 0.1309563056505496 2202 56-57 0.23975632220064824 2202 58-59 0.24835795524857218 2202 60-61 0.21774461730203853 2202 62-63 0.1520861172490342 2202 64-65 0.11012555396616364 2202 66-67 0.11920771511105244 2202 68-69 0.12162129311905545 2202 70-71 0.019449921214466315 2202 72-73 0.13954237413484805 2202 74-75 0.15291206979850358 2202 76-77 -0.15311804209335733 2202 78-79 0.21156652135748288 2202 80-81 0.2586957654020594 2202 82-83 0.3957976787726025 2202 84-85 0.4943387309346363 2202 86-87 0.3485464733959631 2202 88-89 0.5817912926447661 2202 90-91 0.28684135216008144 2202 92-93 0.35475697168858034 2202 94-95 0.40287277793090226 2202 96-97 0.4092870967556621 2202 98-99 0.32416705617693964 2202 100-101 0.30711069991075846 1102 1 0.07634257014328227 1102 2 0.06572990747940821 1102 3 0.049656536589928635 1102 4 0.0798215520405563 1102 5 0.072811168480996 1102 6 0.02773674270152071 1102 7 0.10513605120632263 1102 8 0.08931193705388552 1102 9 0.07750023215565705 1102 10-11 0.09660947494194971 1102 12-13 0.09323228414873341 1102 14-15 0.15455367928989006 1102 16-17 0.15927734885465128 1102 18-19 0.10228878145463227 1102 20-21 0.07389873718402384 1102 22-23 0.12202929663435214 1102 24-25 0.027156986793357873 1102 26-27 0.0775775067973754 1102 28-29 0.10415468555830643 1102 30-31 0.09659611003615964 1102 32-33 0.21111168504511113 1102 34-35 0.13977830560349247 1102 36-37 0.10224375977490041 1102 38-39 0.1325752676636469 1102 40-41 0.1700951184961923 1102 42-43 0.11339930050259284 1102 44-45 0.02624917514903302 1102 46-47 0.14692769651921367 1102 48-49 0.06340649178869029 1102 50-51 0.056974140119109506 1102 52-53 0.10846752664102155 1102 54-55 0.030935630781335988 1102 56-57 0.06810403072517346 1102 58-59 0.13776743172717687 1102 60-61 0.08818110980723048 1102 62-63 0.03228834209828335 1102 64-65 0.06037468938072976 1102 66-67 0.03832827487503465 1102 68-69 0.04707212192854371 1102 70-71 -0.04200155830250196 1102 72-73 -0.08443101166987788 1102 74-75 -0.13375678749694941 1102 76-77 -0.07591684904322449 1102 78-79 0.007455268525220049 1102 80-81 0.0470606748992779 1102 82-83 0.07706747868653707 1102 84-85 0.1240507863925373 1102 86-87 0.12125700707979803 1102 88-89 0.2711459385924897 1102 90-91 0.03266269536550226 1102 92-93 0.04097072261539125 1102 94-95 0.10960896141485676 1102 96-97 0.011660234721205143 1102 98-99 -0.018943351513335926 1102 100-101 0.052564950767568064 2305 1 0.062437320682612096 2305 2 0.0775828563773544 2305 3 0.038017606799606085 2305 4 0.04296517883688722 2305 5 0.04490792794634757 2305 6 0.007184059757989303 2305 7 0.009277463618289516 2305 8 0.01582462410658536 2305 9 0.04097972627155855 2305 10-11 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-0.7579572775459127 1213 90-91 -0.8370717305629753 1213 92-93 -0.9716525714343618 1213 94-95 -0.8874769869983901 1213 96-97 -0.8852416743691123 1213 98-99 -1.0003110301093585 1213 100-101 -0.9100138295394835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 15.0 8 125.0 9 385.0 10 324.0 11 106.0 12 120.0 13 210.0 14 549.0 15 1200.0 16 2329.0 17 3873.0 18 5750.0 19 8080.0 20 10860.0 21 14638.0 22 19184.0 23 25037.0 24 32849.0 25 42242.0 26 55687.0 27 76308.0 28 112931.0 29 154590.0 30 145550.0 31 148793.0 32 195032.0 33 276474.0 34 427013.0 35 755472.0 36 1503033.0 37 2184316.0 38 451776.0 39 473.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.34897440370147 25.893170382167764 21.885076805150174 15.872778408980595 2 29.63655864087158 24.926359708407887 31.09662279402175 14.340458856698787 3 35.731468556686316 19.453413890562572 27.238535845813345 17.57658170693777 4 28.802123394581862 19.317678693645234 31.754298323052105 20.1258995887208 5 33.70259659785159 18.00420234987808 30.767803340603706 17.525397711666628 6 28.718992493829 19.02816451911282 34.82512647017636 17.427716516881823 7 31.85838886281119 21.78212510765817 27.52411452845872 18.835371501071922 8 28.500475709371926 27.72112973012283 25.225969464446813 18.552425096058435 9 30.953925008008625 23.43236783062703 25.979276140425316 19.634431020939026 10-11 24.94765816295253 28.410100359293246 29.156063569300084 17.486177908454152 12-13 23.766551410569946 27.052424495035854 28.577496752975513 20.60352734141869 14-15 23.396719678861615 27.468737810510802 28.611266709178995 20.523275801448584 16-17 24.301192614942536 26.39233134685634 29.3164065449387 19.990069493262425 18-19 22.003594415538597 26.396220529609078 30.982473580549946 20.61771147430238 20-21 23.356586396094315 26.73212453668672 30.4964266202517 19.41486244696727 22-23 21.996215793670977 29.767053814689586 28.70747604256312 19.529254349076318 24-25 21.85711770005487 26.253988536095314 30.685846399063365 21.20304736478645 26-27 21.728243433377546 26.38535704647888 29.23415148533715 22.65224803480642 28-29 22.792346398161833 28.380105912199017 28.681616402146616 20.14593128749254 30-31 20.88718746074571 27.56522447291822 30.113590262472574 21.433997803863495 32-33 20.72823952673078 28.927487226767624 28.968762452436575 21.375510794065022 34-35 22.470235859290998 29.096081573188627 29.159106303464714 19.274576264055664 36-37 22.168653916254534 29.440944623968225 29.458021337101382 18.932380122675863 38-39 21.066973971566483 27.63985344266909 31.82503454588196 19.468138039882472 40-41 21.04851595926435 27.32122255036284 28.719846383309044 22.910415107063763 42-43 21.358316632513723 28.901126765325152 30.13320961892079 19.60734698324033 44-45 20.9237470462289 27.601604354207765 30.41077937785634 21.063869221706998 46-47 22.453008915499968 27.517269245153937 29.01094193437484 21.018779904971254 48-49 21.08725854128541 29.231145128465553 30.007918298843766 19.67367803140527 50-51 22.410480190551652 27.844535084820144 29.40071992557168 20.34426479905653 52-53 20.811407883057253 27.55356413703674 28.400793512162814 23.23423446774319 54-55 22.484202728025448 27.629424926705166 29.755531111421675 20.13084123384771 56-57 21.22320605965107 27.338011539294172 29.42648507190324 22.01229732915152 58-59 21.212252147508863 27.470574514604984 29.557951929707073 21.75922140817908 60-61 22.300175164955828 27.701248665487682 29.452642760791452 20.54593340876504 62-63 20.847675002421006 27.765100721058367 29.48718285831872 21.90004141820191 64-65 22.65841650183243 27.668813738252624 29.39793708310087 20.27483267681407 66-67 21.706789674851898 30.363682603890368 27.754310499670897 20.175217221586834 68-69 21.79461187822616 30.246293699420512 27.756327878825495 20.20276654352783 70-71 21.45880982099696 30.820958182119902 27.808361171035322 19.911870825847817 72-73 21.38830281512054 31.12015261674057 27.46932240436748 20.02222216377141 74-75 21.977663320240364 30.34595603561021 27.30796738739244 20.368413256756988 76-77 22.059018053302516 29.47912169775359 27.816694885599087 20.64516536334481 78-79 21.790370484027573 29.21718310591512 28.506179642982683 20.486266767074625 80-81 21.343081754177767 29.504523982679636 28.333726176480067 20.818668086662527 82-83 20.539276919689208 29.591457949035377 28.546743491072895 21.322521640202524 84-85 20.65282277687711 29.505402158519168 28.54042427890386 21.301350785699867 86-87 20.497995759474303 29.650962173717275 29.029498825649707 20.821543241158707 88-89 20.567413138716628 29.96977937523703 28.890540050988143 20.572267435058194 90-91 21.002758190331363 29.395617855975935 28.417250833303527 21.18437312038918 92-93 20.619054229552766 29.876389530776574 28.085731458711464 21.418824780959195 94-95 20.374850377880392 30.131091822140405 28.03322587995241 21.46083192002679 96-97 20.72325469421993 30.537907226993365 27.66267943824245 21.07615864054426 98-99 20.934656786487775 29.97727214139928 27.681548738108198 21.406522334004748 100-101 21.05718943775365 29.904641565613467 27.267664175586898 21.770504821045986 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 16.0 1 13.5 2 26.5 3 69.0 4 78.0 5 55.5 6 47.5 7 52.0 8 54.5 9 49.0 10 58.5 11 61.5 12 63.0 13 69.0 14 66.0 15 78.5 16 99.0 17 125.0 18 174.0 19 245.5 20 339.0 21 486.5 22 750.5 23 1062.0 24 1501.0 25 2313.0 26 3685.0 27 5892.0 28 8271.0 29 10548.5 30 13436.5 31 17745.0 32 29436.0 33 52024.5 34 89161.0 35 143523.0 36 219920.5 37 316188.0 38 406301.5 39 442965.0 40 451652.0 41 440865.0 42 417953.5 43 428089.0 44 425797.5 45 409863.0 46 408325.0 47 370324.0 48 296701.5 49 243890.0 50 203576.5 51 172722.0 52 151343.5 53 123079.5 54 89490.5 55 61157.0 56 43309.0 57 32428.5 58 26352.0 59 21117.0 60 16174.5 61 12705.5 62 9832.0 63 7518.5 64 6041.0 65 4685.0 66 3430.0 67 2564.0 68 1990.0 69 1513.0 70 1057.5 71 755.0 72 580.0 73 424.0 74 298.5 75 194.0 76 130.5 77 101.0 78 76.0 79 45.5 80 28.5 81 21.5 82 21.0 83 13.5 84 11.0 85 9.5 86 3.5 87 3.0 88 5.0 89 4.5 90 3.0 91 1.5 92 0.0 93 0.5 94 1.0 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1659273087230614 2 0.0 3 2.103579029360554E-4 4 0.021787068518377167 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.40920321835571E-4 12-13 0.0 14-15 0.0 16-17 0.0011194045549097235 18-19 0.0 20-21 0.0 22-23 4.733052816061247E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.315849987168168E-4 38-39 6.686376200467475E-4 40-41 0.004778129509547544 42-43 0.00317790689078398 44-45 9.090466519736679E-4 46-47 0.004282285881198271 48-49 0.007294911562532493 50-51 0.004965949065740451 52-53 0.0015250947962864017 54-55 0.010427741759830175 56-57 0.01919515864291506 58-59 0.023560085128838205 60-61 0.011111404944372355 62-63 5.634586685787199E-4 64-65 0.013695802037586751 66-67 0.03493443745188063 68-69 0.01978866844048464 70-71 0.037203297690690945 72-73 0.030900073384856994 74-75 0.030163820724580803 76-77 0.03876595639821592 78-79 0.017084066831306786 80-81 0.012689089216392769 82-83 9.090466519736679E-4 84-85 0.010750791396481975 86-87 0.02151660835745938 88-89 0.02140391662374364 90-91 0.025333101739299247 92-93 0.04287544828771672 94-95 0.028488470283340075 96-97 0.02938249137081831 98-99 0.037195784908443226 100-101 0.016745991630159555 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6655324.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.717773212191663 #Duplication Level Percentage of deduplicated Percentage of total 1 84.82155225704004 26.055292057378193 2 6.347648183990342 3.8997123469319113 3 2.387721036471781 2.2003641987695817 4 1.2595318714319779 1.5476005752069935 5 0.8053296159991992 1.2368966252662397 6 0.5474329711311899 1.0089553113650493 7 0.45988549284879754 0.9888660791034447 8 0.2670473723793566 0.6562480497328617 9 0.24131745551371842 0.6671461383552196 >10 2.247123611185628 13.879553246718865 >50 0.28567150312056616 6.1191879017784405 >100 0.2560062574401566 17.13925739001684 >500 0.05148512275989116 10.962578390387085 >1k 0.021954489518794914 10.64838357496741 >5k 2.4397462720483057E-4 0.44917737971294625 >10k+ 4.8784541240883206E-5 2.5407807343088615 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 169549 2.54756943463609 TruSeq Adapter, Index 7 (97% over 38bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6751 0.101437585908665 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0012471218531209 0.0 0.0 2 0.0014124030625706576 0.0 0.0 3 0.001562658707524983 0.0 0.0 4 0.0017429654814701734 0.0 0.0 5 0.0021787068518377166 0.0 0.0 6 0.002644499351196125 0.0 0.0 7 0.0031703941085362637 0.0 0.0 8 0.0038014678173444298 0.0 0.0 9 0.004597822735602354 0.0 0.0 10-11 0.00709957922409187 0.0 0.0 12-13 0.009646412406067683 0.0 0.0 14-15 0.012298424539511525 0.0 0.0 16-17 0.014800181028001042 0.0 0.0 18-19 0.01796306235428959 0.0 0.0 20-21 0.02127619932553246 0.0 0.0 22-23 0.02495746262691343 0.0 0.0 24-25 0.028984313911689347 0.0 0.0 26-27 0.03312385693018101 0.0 0.0 28-29 0.03787944809298541 0.0 0.0 30-31 0.06590963865921479 0.0 0.0 32-33 0.07285144945610461 0.0 0.0 34-35 0.07855365118212126 0.0 0.0 36-37 0.08465403036726687 0.0 7.5127822477162646E-6 38-39 0.0915507644706704 0.0 1.5025564495432529E-5 40-41 0.0999124911123786 0.0 1.5025564495432529E-5 42-43 0.10854467791500459 0.0 1.5025564495432529E-5 44-45 0.11864185725593525 0.0 1.5025564495432529E-5 46-47 0.12963305768434413 0.0 1.5025564495432529E-5 48-49 0.14260011984390242 0.0 1.5025564495432529E-5 50-51 0.15619074293002114 0.0 1.5025564495432529E-5 52-53 0.17135153750591256 0.0 1.5025564495432529E-5 54-55 0.18709832909712587 0.0 1.5025564495432529E-5 56-57 0.20530180048334234 0.0 2.2538346743148795E-5 58-59 0.2247599065049275 0.0 3.0051128990865058E-5 60-61 0.24762130288472808 0.0 3.0051128990865058E-5 62-63 0.27302201966425677 0.0 3.0051128990865058E-5 64-65 0.30150297716534913 0.0 3.0051128990865058E-5 66-67 0.33122354373731466 0.0 3.0051128990865058E-5 68-69 0.3651212172390105 0.0 3.0051128990865058E-5 70-71 0.4030682803722253 0.0 3.0051128990865058E-5 72-73 0.44466655567783026 0.0 3.0051128990865058E-5 74-75 0.49099037101724874 0.0 3.0051128990865058E-5 76-77 0.5435197444932809 0.0 3.756391123858132E-5 78-79 0.6000083542138595 0.0 4.507669348629759E-5 80-81 0.6634688258603187 0.0 4.507669348629759E-5 82-83 0.7330071383451805 0.0 4.507669348629759E-5 84-85 0.810756921826796 0.0 4.507669348629759E-5 86-87 0.897026200377322 0.0 4.507669348629759E-5 88-89 0.9948501380248355 0.0 4.507669348629759E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 27680 0.0 66.35339 4 TCGGAAG 27940 0.0 65.923416 3 GAAGAGC 28225 0.0 65.09017 6 GATCGGA 28495 0.0 64.695404 1 ATCGGAA 28865 0.0 63.794403 2 CGACGCG 30 1.2916842E-4 63.43211 1 GGAAGAG 31015 0.0 59.326748 5 GTTACGG 175 0.0 46.214817 1 TTGTGTT 62935 0.0 45.1589 1 GCCGTCT 20420 0.0 44.69113 50-51 ATGCCGT 20500 0.0 44.43685 48-49 TATGCCG 20440 0.0 44.404636 48-49 TGCCGTC 20505 0.0 44.29736 50-51 TCGTATG 20605 0.0 44.232624 44-45 CGTCTTC 20610 0.0 44.188595 52-53 CGTATGC 20480 0.0 44.177437 46-47 TCTCGTA 20360 0.0 43.92545 42-43 CTCGTAT 20525 0.0 43.618282 44-45 CATCTCG 20410 0.0 43.582684 40-41 ATCTCGT 20440 0.0 43.42822 42-43 >>END_MODULE