FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_52260024-1.34100000035820.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_52260024-1.34100000035820.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6655324
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1695492.54756943463609TruSeq Adapter, Index 7 (97% over 38bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC67510.101437585908665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA276800.066.353394
TCGGAAG279400.065.9234163
GAAGAGC282250.065.090176
GATCGGA284950.064.6954041
ATCGGAA288650.063.7944032
CGACGCG301.2916842E-463.432111
GGAAGAG310150.059.3267485
GTTACGG1750.046.2148171
TTGTGTT629350.045.15891
GCCGTCT204200.044.6911350-51
ATGCCGT205000.044.4368548-49
TATGCCG204400.044.40463648-49
TGCCGTC205050.044.2973650-51
TCGTATG206050.044.23262444-45
CGTCTTC206100.044.18859552-53
CGTATGC204800.044.17743746-47
TCTCGTA203600.043.9254542-43
CTCGTAT205250.043.61828244-45
CATCTCG204100.043.58268440-41
ATCTCGT204400.043.4282242-43
Produced by FastQC (version 0.11.2)