FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_52150223-1.3410000003573e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_52150223-1.3410000003573e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3219527
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT1161743.608418255228175TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA183300.069.779894
TCGGAAG184200.069.696793
GATCGGA185400.069.280751
ATCGGAA186900.068.664532
GAAGAGC196450.065.0515066
AGAGCAC197650.064.60858
GAGCACA197500.064.465219
AAGAGCA215350.059.232067
GGAAGAG230400.055.6927385
ATGCCGT139050.044.5388948-49
GCCGTCT139250.044.5333450-51
CGTCTTC140600.044.1074652-53
TGCCGTC138950.044.04833650-51
TCGTATG142700.044.04695544-45
TCTCGTA139550.043.88412542-43
TATGCCG140850.043.81796648-49
AATCTCG139500.043.72827540-41
CGTATGC141200.043.65720446-47
CCGTCTT140600.043.49931352-53
CTCGTAT139550.043.33961544-45
Produced by FastQC (version 0.11.2)