##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_52150223-1.3410000003573e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3219527 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.674316444620594 33.0 33.0 33.0 33.0 33.0 2 32.71521624139198 33.0 33.0 33.0 33.0 33.0 3 32.747948689357166 33.0 33.0 33.0 33.0 33.0 4 36.67068485525979 37.0 37.0 37.0 37.0 37.0 5 36.67331288105365 37.0 37.0 37.0 37.0 37.0 6 36.568627627598715 37.0 37.0 37.0 37.0 37.0 7 36.50224054651506 37.0 37.0 37.0 37.0 37.0 8 36.451067812135136 37.0 37.0 37.0 37.0 37.0 9 36.472078351882125 37.0 37.0 37.0 37.0 37.0 10-11 36.41596529552322 37.0 37.0 37.0 37.0 37.0 12-13 36.38535800445221 37.0 37.0 37.0 37.0 37.0 14-15 38.25883305218437 40.0 37.0 40.0 37.0 40.0 16-17 38.26030749237388 40.0 37.0 40.0 37.0 40.0 18-19 38.247203859448916 40.0 37.0 40.0 37.0 40.0 20-21 38.21463494482264 40.0 37.0 40.0 37.0 40.0 22-23 38.06457019928703 40.0 37.0 40.0 35.0 40.0 24-25 38.0785817295522 40.0 37.0 40.0 37.0 40.0 26-27 38.00827776875299 40.0 37.0 40.0 35.0 40.0 28-29 37.85064545195614 40.0 37.0 40.0 33.0 40.0 30-31 37.78314438735876 40.0 37.0 40.0 33.0 40.0 32-33 37.66732380253373 40.0 37.0 40.0 33.0 40.0 34-35 37.62021921232529 40.0 37.0 40.0 33.0 40.0 36-37 37.537935075556135 40.0 37.0 40.0 33.0 40.0 38-39 37.34856145017575 40.0 37.0 40.0 33.0 40.0 40-41 37.23720689405617 40.0 37.0 40.0 33.0 40.0 42-43 37.06818812204402 40.0 37.0 40.0 33.0 40.0 44-45 36.9383480865357 40.0 37.0 40.0 33.0 40.0 46-47 36.86344748778314 38.5 37.0 40.0 33.0 40.0 48-49 36.68986795265267 37.0 37.0 40.0 33.0 40.0 50-51 36.6167887394639 37.0 37.0 40.0 33.0 40.0 52-53 36.5284200132504 37.0 37.0 40.0 33.0 40.0 54-55 36.27519834435307 37.0 37.0 40.0 33.0 40.0 56-57 36.09102952079607 37.0 37.0 40.0 33.0 40.0 58-59 35.896451248894635 37.0 37.0 40.0 27.0 40.0 60-61 35.66318282157596 37.0 35.0 40.0 27.0 40.0 62-63 35.458694864183464 37.0 33.0 40.0 27.0 40.0 64-65 35.24325157080528 37.0 33.0 40.0 27.0 40.0 66-67 34.81794064780323 37.0 33.0 40.0 27.0 40.0 68-69 34.62030167785517 37.0 33.0 37.0 27.0 40.0 70-71 34.32566119184588 37.0 33.0 37.0 27.0 40.0 72-73 33.826546570350246 37.0 33.0 37.0 27.0 40.0 74-75 33.210834852448826 37.0 33.0 37.0 22.0 40.0 76-77 30.56846611318992 33.0 30.0 35.0 22.0 37.0 78-79 32.78909650392744 37.0 33.0 37.0 22.0 37.0 80-81 33.02766772883098 37.0 33.0 37.0 22.0 37.0 82-83 32.83326976291859 37.0 33.0 37.0 22.0 37.0 84-85 32.64618622549213 37.0 33.0 37.0 22.0 37.0 86-87 32.50423105630112 37.0 33.0 37.0 22.0 37.0 88-89 32.20838573492317 37.0 33.0 37.0 22.0 37.0 90-91 32.147053744230135 35.0 33.0 37.0 22.0 37.0 92-93 31.97812924072387 33.0 33.0 37.0 22.0 37.0 94-95 31.78000448513089 33.0 33.0 37.0 18.5 37.0 96-97 31.66676176345159 33.0 33.0 37.0 15.0 37.0 98-99 31.531440953904095 33.0 33.0 37.0 15.0 37.0 100-101 29.92326916345165 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.17768754591644154 2306 2 0.12142397170986641 2306 3 0.1393186691886399 2306 4 0.13863319761459536 2306 5 0.1587342946786805 2306 6 0.17010860194816502 2306 7 0.22692388877008796 2306 8 0.1881391580491325 2306 9 0.1930156026163914 2306 10-11 0.25643932497657573 2306 12-13 0.23150259324862077 2306 14-15 0.44546624696170056 2306 16-17 0.37115264035778495 2306 18-19 0.35529731291373423 2306 20-21 0.43779967719146384 2306 22-23 0.4077339869522163 2306 24-25 0.41510296699967597 2306 26-27 0.3455879828959425 2306 28-29 0.3606259418343356 2306 30-31 0.3905102168211769 2306 32-33 0.43629179838958265 2306 34-35 0.37848897990906494 2306 36-37 0.27720347033465487 2306 38-39 0.35829630583901206 2306 40-41 0.4161374453961173 2306 42-43 0.440313097573366 2306 44-45 0.3168903730026713 2306 46-47 0.3862687930069626 2306 48-49 0.20441574337993274 2306 50-51 0.17986027257296655 2306 52-53 0.21379207033919556 2306 54-55 0.029204669258987792 2306 56-57 0.23434573458582264 2306 58-59 0.3517336638184716 2306 60-61 0.11490618327614044 2306 62-63 0.14777461394031377 2306 64-65 0.12346098926010995 2306 66-67 0.10174299529521136 2306 68-69 0.25963843663369346 2306 70-71 0.383674340326408 2306 72-73 0.2757577058427856 2306 74-75 0.24356797484774972 2306 76-77 -0.31074460527351633 2306 78-79 0.24117456539102733 2306 80-81 0.22929985295434818 2306 82-83 0.3073725789234132 2306 84-85 0.3833932558427122 2306 86-87 0.2861684361081558 2306 88-89 0.688849791934075 2306 90-91 0.28237507190806355 2306 92-93 0.2759419660863429 2306 94-95 0.2073619444443402 2306 96-97 0.30405745585542476 2306 98-99 0.09557289005291736 2306 100-101 0.10273552473201875 2307 1 0.14122482236901845 2307 2 0.13217121579573643 2307 3 0.10042205656379366 2307 4 0.20831923687062925 2307 5 0.17472334035333148 2307 6 0.20208304428835078 2307 7 0.18436367099722162 2307 8 0.25376020115487563 2307 9 0.2191452446121076 2307 10-11 0.21725216000123027 2307 12-13 0.2188834074885122 2307 14-15 0.37991681565977586 2307 16-17 0.4238324726493303 2307 18-19 0.3260245056945337 2307 20-21 0.3210213508459887 2307 22-23 0.27350564290904344 2307 24-25 0.33981204974825374 2307 26-27 0.34733813889107523 2307 28-29 0.3178216965771341 2307 30-31 0.3819108707236083 2307 32-33 0.3176032157741915 2307 34-35 0.44713277093760695 2307 36-37 0.3020659939163721 2307 38-39 0.4403543104878409 2307 40-41 0.3355705170954977 2307 42-43 0.3757721611646474 2307 44-45 0.3922405866521572 2307 46-47 0.47023887843026557 2307 48-49 0.2977537474449363 2307 50-51 0.36954306421090166 2307 52-53 0.2609641795029489 2307 54-55 0.19534998374883372 2307 56-57 0.1571071587211037 2307 58-59 0.17689686575099728 2307 60-61 0.15293798074154097 2307 62-63 0.1958535018682923 2307 64-65 0.13881054608795296 2307 66-67 0.1681932757215634 2307 68-69 0.16271345661202474 2307 70-71 0.09814576311155321 2307 72-73 0.20947938497081253 2307 74-75 0.21995934202198697 2307 76-77 -0.08622746040420637 2307 78-79 0.06854724234060683 2307 80-81 0.2509229686972745 2307 82-83 0.4302999417480393 2307 84-85 0.3734834593479448 2307 86-87 0.4108099209629472 2307 88-89 0.5400947426177112 2307 90-91 0.3795545703152712 2307 92-93 0.4388948537297068 2307 94-95 0.39387967680411506 2307 96-97 0.32562355583098324 2307 98-99 0.22186463927841515 2307 100-101 0.20971489323450498 2201 1 0.14769469594019569 2201 2 0.15916550270507912 2201 3 0.12643314600330768 2201 4 0.18829417619064515 2201 5 0.12447924684899192 2201 6 0.2091074341432062 2201 7 0.22971060810891686 2201 8 0.21130126814789207 2201 9 0.22373313023686592 2201 10-11 0.2209268550061907 2201 12-13 0.2142971834187435 2201 14-15 0.38892288676014886 2201 16-17 0.4692620444289517 2201 18-19 0.3427249056197397 2201 20-21 0.29304386646681735 2201 22-23 0.4044533451335752 2201 24-25 0.4372913822196409 2201 26-27 0.46280181020980393 2201 28-29 0.3997305972834937 2201 30-31 0.3601497066248882 2201 32-33 0.3923821624989756 2201 34-35 0.3556825568850144 2201 36-37 0.47476968061888414 2201 38-39 0.5964046746563909 2201 40-41 0.5300491034179302 2201 42-43 0.39740461013718686 2201 44-45 0.48263704253322004 2201 46-47 0.5177648895197109 2201 48-49 0.371819665540464 2201 50-51 0.24541626674071892 2201 52-53 0.38482845128549314 2201 54-55 0.43019449764873485 2201 56-57 0.4030271550701059 2201 58-59 0.45445176131229204 2201 60-61 0.2673082381097558 2201 62-63 0.2891608913128607 2201 64-65 0.3543154461243745 2201 66-67 0.19019902071407557 2201 68-69 0.3508251857723508 2201 70-71 0.19439886800396522 2201 72-73 0.21063383337443753 2201 74-75 0.04624509757756812 2201 76-77 -0.12248624559926569 2201 78-79 0.18119225178266873 2201 80-81 0.29045069716993765 2201 82-83 0.39249934429717825 2201 84-85 0.3824836596207106 2201 86-87 0.4025136661521955 2201 88-89 0.6523748745850568 2201 90-91 0.3082004207256972 2201 92-93 0.24260594786587575 2201 94-95 0.34207881704911003 2201 96-97 0.2719348373019379 2201 98-99 0.26208523617986046 2201 100-101 0.16039395967986536 1101 1 0.08478197324976122 1101 2 0.08581503292946735 1101 3 0.11668769093626707 1101 4 0.15042293406857965 1101 5 0.12284132865250541 1101 6 0.13444494232224713 1101 7 0.08615451368213911 1101 8 0.14444847153703932 1101 9 0.19021181072237425 1101 10-11 0.11061543750347624 1101 12-13 0.1715318037571123 1101 14-15 0.2875190864524342 1101 16-17 0.3346877112082538 1101 18-19 0.232578651020809 1101 20-21 0.28487315425822146 1101 22-23 0.3025188297991619 1101 24-25 0.2943255922452721 1101 26-27 0.20742631667224742 1101 28-29 0.329370567465574 1101 30-31 0.40404580882255203 1101 32-33 0.35590752776633394 1101 34-35 0.3888657459592082 1101 36-37 0.4524867158540644 1101 38-39 0.4385353056461838 1101 40-41 0.4984929693351816 1101 42-43 0.339570066663903 1101 44-45 0.4074149374507954 1101 46-47 0.29496445651024317 1101 48-49 0.3102680075200652 1101 50-51 0.17685583757872791 1101 52-53 0.2860607184216164 1101 54-55 0.36867194879912546 1101 56-57 0.4583982822320465 1101 58-59 0.5355286065700469 1101 60-61 0.29979945156983945 1101 62-63 0.3401555179286788 1101 64-65 0.3291690248859851 1101 66-67 0.1967963611483654 1101 68-69 0.32995211736622565 1101 70-71 0.285307757565036 1101 72-73 0.24571270446173088 1101 74-75 0.2706241757618457 1101 76-77 -0.050637534819127694 1101 78-79 0.2702964782233295 1101 80-81 0.41082713781022306 1101 82-83 0.4363333258369053 1101 84-85 0.5099241366398601 1101 86-87 0.499183007487602 1101 88-89 0.7385552113823834 1101 90-91 0.52907553521122 1101 92-93 0.5796915165936838 1101 94-95 0.6600160357395559 1101 96-97 0.6566368922828367 1101 98-99 0.5017175198533685 1101 100-101 0.34875718897533403 2304 1 0.14024517590655705 2304 2 0.11235428634233102 2304 3 0.10899467197175028 2304 4 0.14797918187716164 2304 5 0.12158815268581691 2304 6 0.14883107170427223 2304 7 0.16682094614815668 2304 8 0.14744853464297591 2304 9 0.10433255769421379 2304 10-11 0.14670503241396915 2304 12-13 0.13939957555388105 2304 14-15 0.33989946273256777 2304 16-17 0.26590772112297856 2304 18-19 0.2117473635685343 2304 20-21 0.23855702252406275 2304 22-23 0.31453941448412337 2304 24-25 0.23846409662100143 2304 26-27 0.14736355157246805 2304 28-29 0.20121860296418959 2304 30-31 0.23861586670791013 2304 32-33 0.2192374425800665 2304 34-35 0.2138571152939761 2304 36-37 0.1943700096871197 2304 38-39 0.3824128652753913 2304 40-41 0.31137246972512145 2304 42-43 0.4766455315808855 2304 44-45 0.37004356642196257 2304 46-47 0.3202627670596314 2304 48-49 0.4317126037112331 2304 50-51 0.3482224625009849 2304 52-53 0.19982417441332245 2304 54-55 0.11342571921026234 2304 56-57 0.1601915033043042 2304 58-59 0.33503276936852444 2304 60-61 0.2406981240166033 2304 62-63 0.10557876136473254 2304 64-65 0.08500716793803775 2304 66-67 0.13663201910876666 2304 68-69 0.24239355454831468 2304 70-71 0.17669425242156223 2304 72-73 0.08271175044841783 2304 74-75 0.1292362466794259 2304 76-77 0.017826925569412566 2304 78-79 0.2173904159698239 2304 80-81 0.32380278195481793 2304 82-83 0.4751520838917642 2304 84-85 0.433797197861729 2304 86-87 0.3434769482037865 2304 88-89 0.4457128591304311 2304 90-91 0.40265999643666817 2304 92-93 0.4705679332292192 2304 94-95 0.4092337920635636 2304 96-97 0.4313919686832044 2304 98-99 0.31245243070020123 2304 100-101 0.5508390269172594 2202 1 0.05682540279936177 2202 2 0.09885639967162518 2202 3 0.06023264234729453 2202 4 0.09070970757809249 2202 5 0.09590029417140755 2202 6 0.011780506757332887 2202 7 0.03785837339199816 2202 8 0.10650243150222849 2202 9 0.022233410095360284 2202 10-11 0.10109118148455565 2202 12-13 0.06983780128558692 2202 14-15 0.04431192576983278 2202 16-17 0.0737976266283411 2202 18-19 -0.015521546604801983 2202 20-21 0.13143946943019102 2202 22-23 0.09433371567157423 2202 24-25 0.05600762177851237 2202 26-27 0.07244393949103056 2202 28-29 0.10377252160297701 2202 30-31 0.12018787807325992 2202 32-33 0.18504263924929631 2202 34-35 0.1109464406781342 2202 36-37 0.11117502997780093 2202 38-39 0.1839709234546021 2202 40-41 0.22174859877996056 2202 42-43 0.23809460637895796 2202 44-45 0.11603545300840068 2202 46-47 0.25474620282820837 2202 48-49 0.18703834348360004 2202 50-51 0.19081681253172889 2202 52-53 0.16450197649373166 2202 54-55 0.0845442663079794 2202 56-57 0.20215624615936179 2202 58-59 0.41321374233547914 2202 60-61 0.29972852753788715 2202 62-63 0.24817353984541057 2202 64-65 0.15391141437672928 2202 66-67 0.16612279922326678 2202 68-69 0.17103828886293826 2202 70-71 0.05468949234280984 2202 72-73 -0.08329738838865097 2202 74-75 0.16654880876003375 2202 76-77 -0.17667434799801285 2202 78-79 0.12281680577215326 2202 80-81 0.09770282601375868 2202 82-83 0.22379636119313062 2202 84-85 0.27751137319915387 2202 86-87 0.12374604332467243 2202 88-89 0.39000687004305234 2202 90-91 0.1723363096535664 2202 92-93 0.2798465877821812 2202 94-95 0.22679738295957108 2202 96-97 0.21586422904937308 2202 98-99 0.26277109033743073 2202 100-101 0.3959433080603887 1102 1 0.03757646373298229 1102 2 0.04872558688195028 1102 3 0.07643331754611893 1102 4 0.04333592562672095 1102 5 0.058293618901821276 1102 6 -0.04235978786472572 1102 7 0.07472020715454875 1102 8 0.09362747363232415 1102 9 0.07657406708280945 1102 10-11 0.09595922350106179 1102 12-13 0.06905776601734459 1102 14-15 0.1282283768979937 1102 16-17 0.16572577921657938 1102 18-19 0.15278600590187352 1102 20-21 0.0574526866427334 1102 22-23 0.14152575629523056 1102 24-25 -0.02629457159351034 1102 26-27 0.017162175259436196 1102 28-29 0.04814347501871907 1102 30-31 0.07728631074051151 1102 32-33 0.14365235533433207 1102 34-35 0.11422150878457416 1102 36-37 0.06202386998025844 1102 38-39 0.19356913732467262 1102 40-41 0.13291042118367358 1102 42-43 0.13053856171081435 1102 44-45 0.020073238818056893 1102 46-47 0.1146865782655695 1102 48-49 0.027167610727147462 1102 50-51 -1.0487297011252394E-4 1102 52-53 0.030522865573800573 1102 54-55 0.05601404343278915 1102 56-57 -0.06215504545167505 1102 58-59 0.14862294894891903 1102 60-61 -0.028439329944127678 1102 62-63 0.14970679608764215 1102 64-65 0.07473513459936498 1102 66-67 0.19884126589254691 1102 68-69 0.04514275660850586 1102 70-71 -0.07668174225060653 1102 72-73 -0.009407807417851188 1102 74-75 0.05284149050601883 1102 76-77 -0.005031209012450688 1102 78-79 0.11190227074877157 1102 80-81 0.19804638415887865 1102 82-83 0.32296046281185653 1102 84-85 0.15121176442715267 1102 86-87 0.12273771413451584 1102 88-89 0.3133058848039525 1102 90-91 0.10370586835359319 1102 92-93 0.3081200265478259 1102 94-95 0.5132055151391448 1102 96-97 0.38117975322226627 1102 98-99 0.22097728853603016 1102 100-101 0.1713503173913935 2305 1 0.07369032862464309 2305 2 0.06996695089807048 2305 3 0.058496599972023944 2305 4 0.04832423898461968 2305 5 -0.020569313089495722 2305 6 -0.02399732300973767 2305 7 0.04790570364197322 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-1.3335208078900642 1213 86-87 -1.0160328903040146 1213 88-89 -0.719492268820467 1213 90-91 -0.7631155883875849 1213 92-93 -1.0219122084457126 1213 94-95 -0.9413664137913642 1213 96-97 -0.9235779428766797 1213 98-99 -1.0115406205795345 1213 100-101 -0.8779976702093464 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 5.0 9 24.0 10 19.0 11 18.0 12 36.0 13 101.0 14 336.0 15 838.0 16 1601.0 17 2759.0 18 4024.0 19 5706.0 20 7693.0 21 10238.0 22 13113.0 23 17143.0 24 21526.0 25 26984.0 26 34566.0 27 47730.0 28 67910.0 29 81415.0 30 68827.0 31 73362.0 32 95624.0 33 132682.0 34 194644.0 35 316463.0 36 563183.0 37 970784.0 38 459096.0 39 1076.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.822721296603376 26.470566274648398 19.545961087712246 16.16075134103598 2 29.64134172504222 27.53168400202887 28.45890095035699 14.368073322571917 3 34.91835123350609 21.083098671169196 27.320515281461038 16.678034813863675 4 29.510950002671795 21.73363961610681 28.28267074933236 20.472739631889034 5 34.229344869603516 19.943923439685395 28.81966823076806 17.007063459943026 6 30.85835279530192 20.814330800766697 31.116216761033535 17.211099642897853 7 31.26170397080068 24.641756382226333 25.146302546926925 18.950237100046063 8 28.696296070820342 30.091625260480807 23.086341565080833 18.125737103618018 9 31.69328910737509 24.554072694529353 24.85706751333348 18.895570684762077 10-11 26.334049850124728 29.976777525651805 27.953923430285442 15.735249193938023 12-13 24.076083225890013 29.346671110383603 27.723373650849954 18.853872012876426 14-15 23.38655647242592 29.604752499357822 27.98111337472865 19.027577653487608 16-17 24.860572185566227 27.673914951013316 28.212772334813117 19.252740528607337 18-19 22.105591908376603 27.768908289944456 30.523179336591987 19.602320465086954 20-21 23.938733857489 27.819505784545367 30.43852404405989 17.80323631390574 22-23 21.669795762620655 32.33195015333838 28.01486932730675 17.983384756734207 24-25 21.37435095279524 28.020699935114692 30.38317429858485 20.221774813505213 26-27 21.339578764209772 27.971624403212026 28.23983150319907 22.448965329379128 28-29 23.25116391320837 30.29525765741365 28.119875994206602 18.33370243517138 30-31 20.93012420768641 28.262195036724343 30.338618064082084 20.46906269150717 32-33 20.839008338802564 30.449519448043144 28.18452834841888 20.526943864735408 34-35 20.77783475647199 28.27385824066703 30.393672735156436 20.554634267704543 36-37 22.90653308037417 28.207509134981546 28.226984257143673 20.658973527500613 38-39 22.856442661425287 30.466238940856798 28.132574915173375 18.544743482544536 40-41 22.689380767511018 30.34880468462799 28.212803674495763 18.749010873365233 42-43 20.578234409230728 30.309192242953003 30.23520378353747 18.877369564278794 44-45 20.393241430806917 28.202844045872986 30.47520141338805 20.928713109932044 46-47 22.559981437367735 28.14939626405282 28.170378623650393 21.120243674929046 48-49 20.3029113233593 30.424034228644064 30.2004757567813 19.072578691215337 50-51 22.468947015050897 28.177956197418975 30.166400233088613 19.18669655444152 52-53 20.34971829275376 28.27664244844737 27.99616210956003 23.37747714923884 54-55 22.21675329794772 28.224148305215056 30.35654210876681 19.20255628807042 56-57 20.18997250491971 28.198914580407614 30.252246120189586 21.358866794483088 58-59 20.12603743842242 28.51122877725914 30.157662524187934 21.205071260130502 60-61 22.11374196319085 28.329858061349615 30.263274179932885 19.293125795526645 62-63 20.033744260191106 28.45943506008134 30.132142758969106 21.37467792075845 64-65 22.06767515231041 28.393014907346313 30.214216218029726 19.325093722313554 66-67 20.109654877027424 32.29355081612752 27.995938432073835 19.600855874771224 68-69 20.017735855973292 32.56432393271869 27.90821904232591 19.509721168982104 70-71 19.953079104168523 32.47531262083855 28.03202713898898 19.53958113600395 72-73 19.87476161862584 32.40203294494283 27.974834880702122 19.7483705557292 74-75 19.84737176509699 32.05032925755102 28.188065947825226 19.914233029526766 76-77 19.948924003248255 31.742560570405598 28.139680018419426 20.16883540792672 78-79 19.893401743770276 31.67623070752985 28.23503992852443 20.195327620175448 80-81 19.87269274629474 31.398003380404898 28.28484368966752 20.44446018363284 82-83 19.92971256823082 31.38196073948876 28.106757132214387 20.58156956006603 84-85 19.915947970246613 31.141854807558346 28.309999094494543 20.632198127700498 86-87 20.07108101076079 30.8807847492709 28.332337898869554 20.71579634109876 88-89 20.078133070093752 31.006138028359352 28.208073181017856 20.707655720529036 90-91 20.174652084572173 30.72646083469877 28.252155655843183 20.846731424885874 92-93 20.137910183126714 30.764066600967038 28.134740069818932 20.963283146087317 94-95 20.025839993985066 30.679237345797343 28.22864775151352 21.06627490870407 96-97 20.116320019710308 30.57336209720904 28.212272928728314 21.09804495435234 98-99 20.114082435092623 30.6487327686694 28.13740290323578 21.0997818930022 100-101 20.266131016434834 30.54660181869242 27.994331186921716 21.192935977951027 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8.0 1 7.0 2 7.5 3 11.5 4 13.0 5 8.0 6 7.0 7 14.0 8 21.0 9 23.5 10 30.0 11 35.5 12 39.5 13 64.5 14 87.0 15 137.5 16 227.5 17 350.5 18 628.0 19 1085.0 20 1834.0 21 3001.5 22 4613.0 23 6922.0 24 10141.5 25 14262.5 26 19310.5 27 25343.5 28 32458.5 29 40348.5 30 48607.0 31 57819.0 32 67926.0 33 79043.5 34 91227.5 35 104139.0 36 117884.0 37 131985.0 38 144923.5 39 154557.0 40 161217.5 41 164237.5 42 163709.5 43 161432.0 44 156053.5 45 148418.5 46 139965.5 47 129872.0 48 118549.5 49 106980.5 50 95769.0 51 85892.0 52 75964.5 53 65209.0 54 55288.0 55 46360.0 56 38488.0 57 31666.5 58 25765.0 59 20859.5 60 16809.0 61 13129.5 62 9877.5 63 7457.0 64 5551.5 65 4177.0 66 3171.5 67 2290.0 68 1671.5 69 1221.0 70 871.5 71 623.5 72 466.0 73 335.5 74 236.5 75 190.0 76 163.0 77 117.0 78 79.0 79 52.5 80 42.0 81 31.0 82 25.0 83 24.5 84 19.0 85 12.5 86 7.5 87 8.5 88 7.5 89 4.5 90 3.5 91 1.5 92 1.0 93 1.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16216667852141015 2 0.0 3 1.5530231614768256E-4 4 0.022208231209118606 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.124976432873525E-4 12-13 0.0 14-15 0.0 16-17 9.16283665271327E-4 18-19 0.0 20-21 0.0 22-23 4.1931625359874295E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0010871162130337779 38-39 5.901488013611937E-4 40-41 0.004550357863127099 42-43 0.00329240910233087 44-45 9.007534336565588E-4 46-47 0.004845432263807696 48-49 0.00729920885894108 50-51 0.004674599716045245 52-53 0.0015530231614768258 54-55 0.010187831939287977 56-57 0.018248022147352702 58-59 0.022410124220110593 60-61 0.011010934214870694 62-63 5.124976432873525E-4 64-65 0.013759785210684675 66-67 0.033094923571071154 68-69 0.019381729055230786 70-71 0.035144914144220565 72-73 0.029352137751912005 74-75 0.028637747097632667 76-77 0.036822179158615534 78-79 0.01630674319550667 80-81 0.011989338806601094 82-83 0.0010094650549599365 84-85 0.010265483097361817 86-87 0.020639677816027012 88-89 0.020484375499879328 90-91 0.024382463635186164 92-93 0.04179185327534138 94-95 0.027115784399385376 96-97 0.028156309917574847 98-99 0.03579718387204083 100-101 0.015871896710293158 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3219527.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.26796360105921 #Duplication Level Percentage of deduplicated Percentage of total 1 97.91904827358577 88.38953085410401 2 1.444584806330117 2.6079945743290036 3 0.23551988435666654 0.6377970104529974 4 0.09343948232210408 0.33738367156614035 5 0.07752103376985497 0.34988329273268753 6 0.03791823995012166 0.20536813821802868 7 0.03828610198539231 0.24192059223106838 8 0.02012606686355859 0.14533912568577526 9 0.008956423239999841 0.07276302783215878 >10 0.10663939622484399 1.8417995791387463 >50 0.0140955014193367 0.8511841082113656 >100 0.003594862678766813 0.4923312711397651 >500 1.6503864751803905E-4 0.10395715714976465 >1k 7.062112586316586E-5 0.12918668810556097 >5k 0.0 0.0 >10k+ 3.4267500072676474E-5 3.59356090910291 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 116174 3.608418255228175 TruSeq Adapter, Index 6 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0018015068673131178 0.0 0.0 2 0.0019568091834608003 0.0 0.0 3 0.002484837058362921 0.0 0.0 4 0.002733320764199213 0.0 0.0 5 0.00338559049201948 0.0 3.1060463229536514E-5 6 0.004379525315364648 0.0 6.212092645907303E-5 7 0.0050939159696439884 0.0 6.212092645907303E-5 8 0.00596360894007101 0.0 6.212092645907303E-5 9 0.006429515888514058 0.0 6.212092645907303E-5 10-11 0.009380259895320027 0.0 6.212092645907303E-5 12-13 0.012020399269830631 0.0 6.212092645907303E-5 14-15 0.01495561304502183 0.0 6.212092645907303E-5 16-17 0.017657873345991507 0.0 6.212092645907303E-5 18-19 0.020965812679937145 0.0 6.212092645907303E-5 20-21 0.025003672899776893 0.0 7.765115807384128E-5 22-23 0.029786984237125516 0.0 9.318138968860953E-5 24-25 0.0347877188170809 0.0 1.087116213033778E-4 26-27 0.04058049520938946 0.0 1.397720845329143E-4 28-29 0.046621755307534304 0.0 1.8636277937721907E-4 30-31 0.07884698590817844 0.0 1.8636277937721907E-4 32-33 0.0876681574653668 0.0 1.8636277937721907E-4 34-35 0.0963340267064075 0.0 2.0189301099198732E-4 36-37 0.1060714819288672 0.0 2.1742324260675558E-4 38-39 0.11670969058498346 0.0 2.3295347422152386E-4 40-41 0.1284350154541335 0.0 2.6401393745106036E-4 42-43 0.1418220751060637 0.0 2.9507440068059687E-4 44-45 0.1563583718974868 0.0 3.261348639101334E-4 46-47 0.1722147383761652 0.0 3.7272555875443814E-4 48-49 0.1890029187517297 0.0 3.882557903692064E-4 50-51 0.20532519217885112 0.0 4.0378602198397464E-4 52-53 0.22371298641073672 0.0 4.0378602198397464E-4 54-55 0.24511364557588738 0.0 4.503767168282794E-4 56-57 0.26747717910115365 0.0 4.8143718005781597E-4 58-59 0.2922634287583238 0.0 4.969674116725842E-4 60-61 0.3196276968635455 0.0 4.969674116725842E-4 62-63 0.34922831832129375 0.0 5.280278749021207E-4 64-65 0.38104976289995396 0.0 5.746185697464254E-4 66-67 0.41416021670263986 0.0 5.901488013611937E-4 68-69 0.45239564693819934 0.0 5.901488013611937E-4 70-71 0.49294508168435924 0.0 6.05679032975962E-4 72-73 0.5383710091575564 0.0 6.212092645907302E-4 74-75 0.5898226664972837 0.0 6.212092645907302E-4 76-77 0.6466167235124911 0.0 6.212092645907302E-4 78-79 0.7066100082403409 0.0 6.677999594350351E-4 80-81 0.7728464460773274 0.0 7.454511175088763E-4 82-83 0.8452794463286066 0.0 8.075720439679493E-4 84-85 0.9270927064752058 0.0 8.386325071974858E-4 86-87 1.017338882388624 0.0 8.386325071974858E-4 88-89 1.1181145553368554 0.0 8.541627388122541E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 18330 0.0 69.77989 4 TCGGAAG 18420 0.0 69.69679 3 GATCGGA 18540 0.0 69.28075 1 ATCGGAA 18690 0.0 68.66453 2 GAAGAGC 19645 0.0 65.051506 6 AGAGCAC 19765 0.0 64.6085 8 GAGCACA 19750 0.0 64.46521 9 AAGAGCA 21535 0.0 59.23206 7 GGAAGAG 23040 0.0 55.692738 5 ATGCCGT 13905 0.0 44.53889 48-49 GCCGTCT 13925 0.0 44.53334 50-51 CGTCTTC 14060 0.0 44.10746 52-53 TGCCGTC 13895 0.0 44.048336 50-51 TCGTATG 14270 0.0 44.046955 44-45 TCTCGTA 13955 0.0 43.884125 42-43 TATGCCG 14085 0.0 43.817966 48-49 AATCTCG 13950 0.0 43.728275 40-41 CGTATGC 14120 0.0 43.657204 46-47 CCGTCTT 14060 0.0 43.499313 52-53 CTCGTAT 13955 0.0 43.339615 44-45 >>END_MODULE