FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_50331580-1.341000000357a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_50331580-1.341000000357a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3136629
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT732842.3363936251306736TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG108000.072.6591953
CGGAAGA109100.071.708924
GCGACGA200.002074209371.2517553
GATCGGA111000.071.0260541
ATCGGAA111600.070.357922
GAAGAGC118400.065.903296
CGCGATA352.7883492E-454.287053
GGAAGAG153300.051.2406435
TTGTGTT227650.050.9964941
AGAGCAC157800.049.2978678
GAGCACA159200.049.01359
TCGGACG502.8277107E-547.501173
GCCGTCT85850.045.20171450-51
AAGAGCA172250.045.1898577
TCGTATG86900.044.95401444-45
ATGCCGT86200.044.93266748-49
TATGCCG85750.044.9192248-49
TGCCGTC85700.044.8928750-51
TCTCGTA86450.044.85802542-43
CGTATGC86150.044.8488446-47
Produced by FastQC (version 0.11.2)