FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_50330665-2.34100000035758.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_50330665-2.34100000035758.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2529953
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT200860.7939277923344821TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC75920.3000846260780338No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG67260.26585474117503366No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA35230.13925159874511503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCG353.5497578E-881.5704651
CGCGTAG301.2903629E-463.44371
TCGGAAG40750.058.515333
CGGAAGA41200.057.875074
GATCGGA42050.056.9182781
ATCGGAA42200.056.5047382
CGTCCCG352.7885856E-454.285713
GAAGAGC46250.051.3391726
GGTCCGG459.54513E-442.2957951
TGCCGTC27100.042.0676750-51
GCCGTCT27700.041.75665350-51
ATGCCGT27550.041.6358748-49
CGTATGC27400.041.5150646-47
TATGCCG27450.041.3549748-49
AATCTCG27700.041.06462540-41
TCGTATG28150.040.91554344-45
CCGTCTT28100.040.74205852-53
GGCCGCC704.3122545E-640.7142832
GGAAGAG59600.040.5566525
TCTCGTA28450.040.23407742-43
Produced by FastQC (version 0.11.2)