##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_50330665-1.341000000356c2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2871118 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65911188603185 33.0 33.0 33.0 33.0 33.0 2 32.678335756315136 33.0 33.0 33.0 33.0 33.0 3 32.723203644016024 33.0 33.0 33.0 33.0 33.0 4 36.624909530015834 37.0 37.0 37.0 37.0 37.0 5 36.647159399230546 37.0 37.0 37.0 37.0 37.0 6 36.55544739018041 37.0 37.0 37.0 37.0 37.0 7 36.373360830171386 37.0 37.0 37.0 37.0 37.0 8 36.34199639304271 37.0 37.0 37.0 37.0 37.0 9 36.425587175448726 37.0 37.0 37.0 37.0 37.0 10-11 36.32493648815549 37.0 37.0 37.0 35.0 37.0 12-13 36.31719455626693 37.0 37.0 37.0 35.0 37.0 14-15 38.14912918939591 40.0 37.0 40.0 35.0 40.0 16-17 38.15161602553431 40.0 37.0 40.0 37.0 40.0 18-19 38.10846523897659 40.0 37.0 40.0 37.0 40.0 20-21 38.15598592603996 40.0 37.0 40.0 37.0 40.0 22-23 37.83263366395947 40.0 37.0 40.0 33.0 40.0 24-25 38.02108046412582 40.0 37.0 40.0 35.0 40.0 26-27 37.94580926315115 40.0 37.0 40.0 33.0 40.0 28-29 37.70110249735469 40.0 37.0 40.0 33.0 40.0 30-31 37.611566295777465 40.0 37.0 40.0 33.0 40.0 32-33 37.57516044272649 40.0 37.0 40.0 33.0 40.0 34-35 37.41029679030956 40.0 37.0 40.0 33.0 40.0 36-37 37.3675428874745 40.0 37.0 40.0 33.0 40.0 38-39 37.33466266450909 40.0 37.0 40.0 33.0 40.0 40-41 37.271715060126404 40.0 37.0 40.0 33.0 40.0 42-43 36.574279427038526 37.0 37.0 40.0 33.0 40.0 44-45 36.413702954737495 37.0 37.0 40.0 33.0 40.0 46-47 36.743222500781926 37.0 37.0 40.0 33.0 40.0 48-49 36.5234776139469 37.0 37.0 40.0 33.0 40.0 50-51 36.522813412754196 37.0 37.0 40.0 33.0 40.0 52-53 36.49508501566289 37.0 37.0 40.0 33.0 40.0 54-55 36.14912257176473 37.0 37.0 40.0 33.0 40.0 56-57 36.01108801519129 37.0 37.0 40.0 33.0 40.0 58-59 35.83643201010895 37.0 37.0 40.0 33.0 40.0 60-61 35.580681114464824 37.0 33.0 40.0 27.0 40.0 62-63 35.17813844641704 37.0 33.0 37.0 27.0 40.0 64-65 34.8751467198492 37.0 33.0 37.0 27.0 40.0 66-67 34.05353750699205 37.0 33.0 37.0 27.0 40.0 68-69 33.812484021903664 37.0 33.0 37.0 27.0 37.0 70-71 33.30410139882791 37.0 33.0 37.0 27.0 37.0 72-73 31.91740604182761 35.0 33.0 37.0 18.5 37.0 74-75 30.693491176607857 33.0 27.0 37.0 15.0 37.0 76-77 28.08113529294163 30.0 24.5 33.0 15.0 35.0 78-79 30.161009752995174 33.0 27.0 37.0 15.0 37.0 80-81 30.430257133283966 33.0 27.0 37.0 15.0 37.0 82-83 30.18987411872309 33.0 27.0 37.0 6.0 37.0 84-85 30.01680338460488 33.0 27.0 37.0 6.0 37.0 86-87 29.886436572791503 33.0 27.0 37.0 6.0 37.0 88-89 29.64616414233062 33.0 27.0 37.0 6.0 37.0 90-91 29.609341726811646 33.0 27.0 37.0 6.0 37.0 92-93 29.403443884925665 33.0 27.0 37.0 6.0 37.0 94-95 29.174101168952305 33.0 27.0 37.0 6.0 37.0 96-97 29.044652814687517 33.0 27.0 37.0 6.0 37.0 98-99 28.914561157012706 33.0 27.0 37.0 6.0 37.0 100-101 27.407440411714184 33.0 21.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.1618921667958375 2306 2 0.17839300911484912 2306 3 0.1319360326924297 2306 4 0.1767101674738214 2306 5 0.17949498061135216 2306 6 0.19518107266333118 2306 7 0.23732158212830967 2306 8 0.21164018031715415 2306 9 0.23648143760804174 2306 10-11 0.2679948031793984 2306 12-13 0.24072370387348485 2306 14-15 0.4786519840968495 2306 16-17 0.491690014541156 2306 18-19 0.5399191724324055 2306 20-21 0.459545322237382 2306 22-23 0.5269199888451084 2306 24-25 0.5376443758546756 2306 26-27 0.5007546464091845 2306 28-29 0.4226311613346425 2306 30-31 0.5345837171651127 2306 32-33 0.43799585801517793 2306 34-35 0.558554289760778 2306 36-37 0.4839467943457123 2306 38-39 0.5317395229413933 2306 40-41 0.5655878115991584 2306 42-43 0.5590189740883034 2306 44-45 0.6498441571658304 2306 46-47 0.6263327251296431 2306 48-49 0.5861780922931601 2306 50-51 0.6734923504658994 2306 52-53 0.6555576266943675 2306 54-55 0.5805042526368922 2306 56-57 0.4167478807163647 2306 58-59 0.5553994942873928 2306 60-61 0.5689643378298612 2306 62-63 0.6426211129790644 2306 64-65 0.6417288620459445 2306 66-67 0.5615860471110423 2306 68-69 0.3796608342399068 2306 70-71 0.14290835620758457 2306 72-73 -0.01793398073085939 2306 74-75 0.05519562207341622 2306 76-77 -0.01987868278746774 2306 78-79 0.1205412816038347 2306 80-81 0.39729234550042847 2306 82-83 0.6370827042896394 2306 84-85 0.5789030747394222 2306 86-87 0.5023339479242921 2306 88-89 0.8351036619747099 2306 90-91 0.5827688695442355 2306 92-93 0.660558291664401 2306 94-95 0.6374344470563891 2306 96-97 0.5656943739735389 2306 98-99 0.38101206127710796 2306 100-101 0.29168260726134676 2307 1 0.15491188252256904 2307 2 0.16723299077463594 2307 3 0.12794336877679058 2307 4 0.13832043799192917 2307 5 0.1341096833470985 2307 6 0.17771079908852272 2307 7 0.27733258625485036 2307 8 0.2112849915658188 2307 9 0.19186276115993195 2307 10-11 0.23704080654177773 2307 12-13 0.2464333172007045 2307 14-15 0.4885718985091998 2307 16-17 0.5058125882840869 2307 18-19 0.37284351906239266 2307 20-21 0.42698136075182447 2307 22-23 0.42879833211272 2307 24-25 0.4514765629248245 2307 26-27 0.4638657575202956 2307 28-29 0.5202698591796704 2307 30-31 0.44578805768169616 2307 32-33 0.47137198517397394 2307 34-35 0.4475840926035062 2307 36-37 0.4995381133125463 2307 38-39 0.5311761727962008 2307 40-41 0.5859805977828643 2307 42-43 0.5867490673177116 2307 44-45 0.5795048632639492 2307 46-47 0.683860770368824 2307 48-49 0.5314270606562914 2307 50-51 0.6219924268834447 2307 52-53 0.507262654968855 2307 54-55 0.468831625401684 2307 56-57 0.54838077082794 2307 58-59 0.6475691412383284 2307 60-61 0.6135512245790622 2307 62-63 0.6324077551921121 2307 64-65 0.5916958496663014 2307 66-67 0.5213653532397302 2307 68-69 0.5140343632021569 2307 70-71 0.23064120046862513 2307 72-73 0.03831422345682256 2307 74-75 0.10242594455545984 2307 76-77 -0.102981846473849 2307 78-79 0.2967341594886008 2307 80-81 0.44341522491354013 2307 82-83 0.6321873963269304 2307 84-85 0.6354728439584356 2307 86-87 0.5535489868972405 2307 88-89 0.7214527373224122 2307 90-91 0.6230143227002785 2307 92-93 0.5142416173559781 2307 94-95 0.5122533374736307 2307 96-97 0.5184230916871293 2307 98-99 0.562251553864602 2307 100-101 0.5405779152240591 2201 1 0.175040914780638 2201 2 0.10164159292517638 2201 3 0.12454551931442381 2201 4 0.2099763519704254 2201 5 0.16044660090982177 2201 6 0.1795889352892388 2201 7 0.10924656474947625 2201 8 0.2524749557235211 2201 9 0.2132964529162038 2201 10-11 0.2748759319898042 2201 12-13 0.25369496594622376 2201 14-15 0.3967511099787302 2201 16-17 0.4116477137321155 2201 18-19 0.3841983577720711 2201 20-21 0.4328523284937589 2201 22-23 0.40431804537436733 2201 24-25 0.4553045199140726 2201 26-27 0.3813998076995091 2201 28-29 0.45306555810440585 2201 30-31 0.4180819644917264 2201 32-33 0.4221963579338279 2201 34-35 0.4077776409906022 2201 36-37 0.3395309448537702 2201 38-39 0.5906672122227263 2201 40-41 0.5537583755606406 2201 42-43 0.4012508594324018 2201 44-45 0.4791822826187868 2201 46-47 0.5546923115874662 2201 48-49 0.47202921119392727 2201 50-51 0.4688454734784244 2201 52-53 0.44605118543480415 2201 54-55 0.5102724856167384 2201 56-57 0.41496858444801177 2201 58-59 0.5320207541415556 2201 60-61 0.40417488049303785 2201 62-63 0.555841446948385 2201 64-65 0.6902549894512475 2201 66-67 0.5723044213400854 2201 68-69 0.5349734313025181 2201 70-71 0.3169924549489167 2201 72-73 0.17567623062528526 2201 74-75 0.1581105323690828 2201 76-77 -0.09261625507599902 2201 78-79 0.33461946414809773 2201 80-81 0.4668345797522484 2201 82-83 0.5844096185491523 2201 84-85 0.6057667507684705 2201 86-87 0.47161350302627625 2201 88-89 0.5470943143833402 2201 90-91 0.32495697503002674 2201 92-93 0.5040982481803518 2201 94-95 0.48343613317255674 2201 96-97 0.45931198057601463 2201 98-99 0.42952753952768674 2201 100-101 0.3209148327867801 1101 1 0.13328645993974675 1101 2 0.132790800105262 1101 3 0.07478042083899794 1101 4 0.11218926828266973 1101 5 0.09219036849471962 1101 6 0.14349218290641375 1101 7 0.20202765048744453 1101 8 0.2088770582775581 1101 9 0.19832208051985845 1101 10-11 0.11788101388908245 1101 12-13 0.2304490936262198 1101 14-15 0.25040331613372047 1101 16-17 0.2943552973217294 1101 18-19 0.26332898221317436 1101 20-21 0.30135458590395814 1101 22-23 0.2658932160432954 1101 24-25 0.31881124852320397 1101 26-27 0.2607952143605061 1101 28-29 0.27425904105931664 1101 30-31 0.30258544330261117 1101 32-33 0.33325614113520885 1101 34-35 0.3401020101153449 1101 36-37 0.34361609393008763 1101 38-39 0.4476228710157244 1101 40-41 0.4428726419568534 1101 42-43 0.46737967358320276 1101 44-45 0.5909923548122933 1101 46-47 0.5855614690390993 1101 48-49 0.5167603939896281 1101 50-51 0.47143484293032145 1101 52-53 0.34892278568781165 1101 54-55 0.40589856252135803 1101 56-57 0.4572006941995852 1101 58-59 0.586245273760305 1101 60-61 0.500639797939435 1101 62-63 0.4769049379732664 1101 64-65 0.396089358794093 1101 66-67 0.5305778842291389 1101 68-69 0.37347677924903167 1101 70-71 0.126488845273677 1101 72-73 0.20698945446876849 1101 74-75 0.33581517151061036 1101 76-77 0.18101544899606026 1101 78-79 0.5355058520736797 1101 80-81 0.7161359821819637 1101 82-83 0.8799606665545632 1101 84-85 0.747525131540133 1101 86-87 0.5640290409878439 1101 88-89 0.8208581914580861 1101 90-91 0.6509985462747672 1101 92-93 0.6696957530332384 1101 94-95 0.6996637499144676 1101 96-97 0.731644637776828 1101 98-99 0.659761132408665 1101 100-101 0.4058702298510575 2304 1 0.09060587159360978 2304 2 0.09138599623911858 2304 3 0.14043985589623276 2304 4 0.12155853323002219 2304 5 0.13698660242074112 2304 6 0.13298220163861174 2304 7 0.13122485791761562 2304 8 0.12039870481067538 2304 9 0.11521430942951127 2304 10-11 0.13373141283894086 2304 12-13 0.1718410715592782 2304 14-15 0.29763877334656996 2304 16-17 0.28149460753987654 2304 18-19 0.28060464318416933 2304 20-21 0.31064412421786614 2304 22-23 0.28926151192015936 2304 24-25 0.24275837400991662 2304 26-27 0.24158212494157993 2304 28-29 0.2638686101554768 2304 30-31 0.3124261008771114 2304 32-33 0.29803604970688724 2304 34-35 0.28687058752935 2304 36-37 0.3123369683736925 2304 38-39 0.3119557720676056 2304 40-41 0.37425017883933265 2304 42-43 0.3995286665891058 2304 44-45 0.3073815221242171 2304 46-47 0.5293575177016407 2304 48-49 0.5079001285719826 2304 50-51 0.3469115005238237 2304 52-53 0.28827852798473685 2304 54-55 0.4376903295390733 2304 56-57 0.35837972997443757 2304 58-59 0.4014941997863346 2304 60-61 0.2840086796922563 2304 62-63 0.24558131751322065 2304 64-65 0.26056548276545044 2304 66-67 0.21478559784030438 2304 68-69 0.3151517194342688 2304 70-71 0.28759254056750905 2304 72-73 -0.11533709062072006 2304 74-75 0.08087299113365631 2304 76-77 0.13007670153551842 2304 78-79 0.18547212462000573 2304 80-81 0.33042225067466546 2304 82-83 0.49551812752651614 2304 84-85 0.46406092618586214 2304 86-87 0.4485919221041108 2304 88-89 0.7122588783580603 2304 90-91 0.5155004012847186 2304 92-93 0.5490711775953763 2304 94-95 0.5116834701824509 2304 96-97 0.3457444552052138 2304 98-99 0.4345908721055629 2304 100-101 0.5856671684116712 2202 1 0.0537227209683806 2202 2 0.11014710120408466 2202 3 0.0141437777747484 2202 4 0.016862360282317468 2202 5 -0.022441782228902696 2202 6 -0.05324387030479727 2202 7 0.03941643083562951 2202 8 0.05815343055695621 2202 9 0.04082152053280197 2202 10-11 0.075734910154587 2202 12-13 0.05817786389463464 2202 14-15 0.03794033750033776 2202 16-17 -0.001153839800437595 2202 18-19 0.04312317141413047 2202 20-21 0.13196448958618845 2202 22-23 0.18985576224223166 2202 24-25 0.16005631752604899 2202 26-27 0.043699431685261914 2202 28-29 0.01952855038621948 2202 30-31 0.14270182727951664 2202 32-33 0.15392907447186133 2202 34-35 0.0855830701086262 2202 36-37 -0.00944944633298661 2202 38-39 0.06014021505932021 2202 40-41 0.16907023650133368 2202 42-43 0.3258828437321597 2202 44-45 0.20573696960342147 2202 46-47 0.29268302667421153 2202 48-49 0.14950579276263198 2202 50-51 0.18399208605180917 2202 52-53 0.1460695738509301 2202 54-55 0.02877947816241999 2202 56-57 0.14645524253891296 2202 58-59 0.1748840696636833 2202 60-61 0.22469948633774806 2202 62-63 0.16107612601692978 2202 64-65 0.11101946929820627 2202 66-67 -0.04187783012768165 2202 68-69 0.0419005867877047 2202 70-71 -0.27224496176722823 2202 72-73 -0.3145243927667636 2202 74-75 -0.31336359191352514 2202 76-77 -0.46401303256792303 2202 78-79 -0.44388530199742604 2202 80-81 -0.26258886506601087 2202 82-83 -0.1586859847228297 2202 84-85 -0.22600653572800056 2202 86-87 -0.04420152435019986 2202 88-89 0.1695727100558777 2202 90-91 -0.1756566043617518 2202 92-93 -0.0808546675792634 2202 94-95 -0.08659891201512337 2202 96-97 -0.09250550408656011 2202 98-99 -0.1694883916023393 2202 100-101 0.04079996703046618 1102 1 0.06173490098076684 1102 2 0.06800027271455633 1102 3 0.07332302856687534 1102 4 0.06794186397817725 1102 5 0.046232737997840445 1102 6 0.028100954716464344 1102 7 0.12298151857479667 1102 8 0.15782281277464705 1102 9 0.0842044187784623 1102 10-11 0.08532600379114541 1102 12-13 9.834438746096907E-4 1102 14-15 0.1560905376695345 1102 16-17 0.1764372715993261 1102 18-19 0.0942391035719865 1102 20-21 0.12738165029217896 1102 22-23 0.12040528108123283 1102 24-25 0.09140996864324791 1102 26-27 0.19167031778088273 1102 28-29 0.21087789392451128 1102 30-31 0.19138704429045816 1102 32-33 0.2898374212941306 1102 34-35 0.17440204410550564 1102 36-37 0.13342374487244513 1102 38-39 0.13907555752295053 1102 40-41 0.2411995626760941 1102 42-43 0.10087936047732171 1102 44-45 0.029714780021102172 1102 46-47 0.20570441857729804 1102 48-49 0.19236661910725417 1102 50-51 0.2106728467169532 1102 52-53 0.14933793546106955 1102 54-55 0.2008743326039948 1102 56-57 0.25839380014389945 1102 58-59 0.3009509725810702 1102 60-61 0.1676547352558586 1102 62-63 0.18737192457322038 1102 64-65 0.11015621883025517 1102 66-67 0.170378744481134 1102 68-69 0.3327292600534051 1102 70-71 0.05023005316496665 1102 72-73 -0.17569663430647608 1102 74-75 -0.1840916088285418 1102 76-77 -0.081626797613918 1102 78-79 0.004830431656532852 1102 80-81 0.046179611449915114 1102 82-83 0.30201367211412133 1102 84-85 0.15788471511297786 1102 86-87 0.08861956235869783 1102 88-89 0.319656863272467 1102 90-91 0.061716458060395496 1102 92-93 0.11552645267990158 1102 94-95 0.20641186081781981 1102 96-97 -0.03726420472981218 1102 98-99 -0.08047374472388569 1102 100-101 0.12458008677671373 2305 1 0.06477011333686278 2305 2 0.044667085677978946 2305 3 0.035545009081417334 2305 4 0.06685939522684237 2305 5 0.002247156496878233 2305 6 -0.006574301477776601 2305 7 0.00913809299181878 2305 8 0.08123773517058197 2305 9 0.06457902751415645 2305 10-11 0.03973637381379547 2305 12-13 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sequence quality scores pass #Quality Count 7 1.0 8 7.0 9 15.0 10 20.0 11 20.0 12 39.0 13 109.0 14 318.0 15 772.0 16 1601.0 17 2684.0 18 3475.0 19 4540.0 20 6182.0 21 8454.0 22 11557.0 23 15649.0 24 21391.0 25 29082.0 26 40245.0 27 60919.0 28 108539.0 29 180114.0 30 134089.0 31 68487.0 32 80920.0 33 114007.0 34 177946.0 35 315293.0 36 582401.0 37 771909.0 38 130299.0 39 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.86907800572311 19.794545707614848 19.923617441149393 12.412758845512647 2 29.215831602880826 30.821791371862805 28.819748961902647 11.142628063353719 3 37.31360438520141 14.245008291215491 34.590338025941875 13.851049297641227 4 29.461109384775952 13.814010069101403 28.436049501073658 28.288831045048983 5 43.69315367741765 12.306948025124708 29.379321922679598 14.62057637477805 6 38.21420784516694 13.210324340553054 33.70972561907939 14.865742195200616 7 32.21511620212057 28.110025432601514 23.2422004250609 16.432657940217016 8 27.839573295141474 37.45603628969621 18.833952488194495 15.870437926967822 9 42.43716210897636 21.407305446867735 19.126207978912742 17.029324465243157 10-11 31.125719519146223 30.669291907652863 23.112105232086012 15.092883341114899 12-13 24.194878092784762 29.03097330029626 22.668573705434607 24.10557490148437 14-15 23.35668544448539 29.389718569560706 22.9216806832739 24.331915302680002 16-17 29.426391267973163 23.149045343817637 23.870740897346856 23.55382249086234 18-19 21.10158830114262 23.277256455499217 31.156695754058177 24.464459489299987 20-21 27.32621926371539 24.19132546972991 31.151297160200315 17.331158106354387 22-23 20.3525259237897 37.674444848469655 24.2472418313766 17.72578739636405 24-25 20.756513664711797 22.259708587386516 32.17838834906821 24.805389398833487 26-27 19.30660808785985 22.36961350944127 25.547173609722762 32.776604792976116 28-29 25.902993189412626 30.55752149511096 23.915979768159996 19.623505547316412 30-31 17.42502398020562 24.726239046949654 31.130103325603475 26.718633647241248 32-33 17.91518147286179 30.576555892164652 24.1908900992575 27.31737253571605 34-35 19.064890401578758 30.234215382300555 31.410934695125732 19.289959520994955 36-37 17.81596644626303 32.20787462348873 32.01723314023634 17.958925790011897 38-39 17.78781534483437 25.2730744943668 30.002058442347767 26.93705171845106 40-41 26.144876541092042 24.15090271581907 22.799915917081144 26.904304826007746 42-43 19.64444131742221 31.30671525316167 30.211359612626964 18.83748381678916 44-45 18.090986524133385 25.350462915636207 29.959017385524096 26.59953317470631 46-47 25.960341806273323 24.501033269789477 23.91032966757379 25.628295256363415 48-49 18.837308250361342 30.785553721247894 31.442682491213112 18.934455537177648 50-51 25.123938645276507 24.030669698851646 29.6313241102245 21.214067545647353 52-53 17.528364811089922 24.724658882126032 23.426207953466907 34.320768353317135 54-55 25.538548407266358 24.638056248060224 31.162013348803946 18.661381995869473 56-57 19.005123013639075 24.470994671452697 29.922792498787697 26.601089816120528 58-59 18.125737254768072 24.190474232924082 30.16210721260738 27.52168129970046 60-61 24.313396005846464 23.78404804799784 31.74258986009455 20.15996608606115 62-63 17.80604255366577 23.555364069013173 31.61188170125499 27.02671167606607 64-65 25.94582421320436 25.203046423524768 30.147850157652613 18.703279205618262 66-67 19.51960615230241 38.1471667807724 23.20597043006538 19.12725663685981 68-69 18.635743073039084 36.6861471929335 24.876496180709054 19.80161355331836 70-71 18.12573353446499 38.9705770909554 23.770810950932436 19.132878423647174 72-73 19.97626700800553 38.379247777035296 23.367834200732617 18.276651014226555 74-75 21.302406183107387 36.04771603527471 23.556216971771317 19.093660809846593 76-77 20.70667775601642 34.24004563032814 24.07938826928444 20.973888344371 78-79 20.411902039758463 34.45235442762819 24.596064841425274 20.53967869118808 80-81 19.67214542379016 34.65233617155351 24.716446495163556 20.95907190949278 82-83 19.2900840848926 33.847377987392576 24.879274797189147 21.983263130525675 84-85 20.053190516622035 32.805503668386564 24.817991422288987 22.323314392702414 86-87 18.907106337972234 33.1457010396766 25.58095988584599 22.366232736505175 88-89 18.488060666529176 33.53253705262336 25.698725523530296 22.28067675731716 90-91 19.428150781404867 32.25119294929256 24.990933341508715 23.329722927793863 92-93 19.943161653908064 32.116505368579006 24.248704699320726 23.691628278192205 94-95 20.23940523735756 33.238140744860814 23.71384181179704 22.80861220598459 96-97 19.79668665123157 33.93221994158447 23.02824950087944 23.24284390630452 98-99 19.752227195742016 32.18556985515083 24.67162168141824 23.390581267688912 100-101 20.734901553415035 33.09396187544045 23.887209241971224 22.283927329173288 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1395.0 1 824.5 2 365.0 3 571.5 4 541.5 5 362.5 6 334.5 7 364.0 8 391.5 9 405.5 10 365.5 11 318.5 12 310.0 13 311.0 14 293.0 15 292.0 16 291.0 17 308.0 18 319.0 19 338.0 20 392.0 21 441.5 22 473.5 23 488.0 24 579.5 25 706.5 26 869.0 27 1159.5 28 1514.5 29 1999.5 30 2602.5 31 3655.0 32 6098.5 33 10714.5 34 18971.0 35 33458.0 36 54640.5 37 83534.0 38 121357.0 39 153447.0 40 171444.5 41 173135.0 42 187734.0 43 218197.5 44 208726.5 45 178958.5 46 173271.0 47 168147.5 48 145782.0 49 121086.0 50 99530.5 51 86408.0 52 81659.0 53 82794.5 54 74413.5 55 48242.0 56 28854.0 57 23534.5 58 22592.5 59 19981.0 60 15608.0 61 11710.0 62 7847.0 63 4902.0 64 3334.0 65 2262.5 66 1504.5 67 1186.0 68 914.5 69 648.0 70 458.0 71 341.0 72 256.0 73 176.5 74 113.5 75 68.0 76 56.5 77 43.0 78 28.5 79 20.5 80 11.5 81 9.5 82 6.0 83 3.5 84 2.5 85 2.0 86 2.0 87 1.0 88 1.0 89 0.5 90 1.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.156559221878028 2 0.0 3 2.4380746454865317E-4 4 0.019295619337136267 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.746890235789682E-4 12-13 0.0 14-15 0.0 16-17 0.0011145484093652716 18-19 0.0 20-21 0.0 22-23 3.482963779266474E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.662520314386242E-4 38-39 5.224445668899711E-4 40-41 0.0049632233854547255 42-43 0.003030178487961832 44-45 7.836668503349566E-4 46-47 0.00470200110200974 48-49 0.0076451054954899105 50-51 0.004910978928765728 52-53 0.001497674425084584 54-55 0.010414061700006756 56-57 0.01887766368362429 58-59 0.023405516596670704 60-61 0.011215143369238047 62-63 4.8761492909730634E-4 64-65 0.01419307740051088 66-67 0.03388923757226279 68-69 0.019469767526099586 70-71 0.035682963918585026 72-73 0.029674851399350357 74-75 0.02946587357259437 76-77 0.03820811265855322 78-79 0.016857544691649736 80-81 0.01266057333763363 82-83 0.0010971335904689393 84-85 0.010640454345659077 86-87 0.02124607905352549 88-89 0.021106760502354834 90-91 0.02476387247058463 92-93 0.04264889147711797 94-95 0.027741806501857466 96-97 0.028542888171088753 98-99 0.035839697288652016 100-101 0.016544077951515752 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2871118.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.645357746804343 #Duplication Level Percentage of deduplicated Percentage of total 1 76.05147838334524 18.743158919309007 2 9.86639729425402 4.863217819779854 3 3.9546241863761837 2.923893834822183 4 2.0251853891654217 1.9964567367833197 5 1.3423370484121446 1.6541188387453363 6 1.0035242827242925 1.4839328973207249 7 0.7409459475832957 1.2782614564464683 8 0.5656799898703128 1.1153108576450013 9 0.39756916566377026 0.8818410885193926 >10 3.301939254691813 16.076524598664925 >50 0.39024202312373957 6.712672138539941 >100 0.3174468737931118 16.06601739485872 >500 0.028920131083242755 4.720730585071633 >1k 0.012287276997350563 5.372889774042408 >5k 7.113764580660622E-4 1.221813951587769 >10k+ 7.113764579179829E-4 14.889159107863184 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 362624 12.630062574927258 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 20633 0.7186399165760516 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 19223 0.6695301272883942 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11488 0.4001228789621325 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 10657 0.3711794499564281 TruSeq Adapter, Index 27 (97% over 39bp) TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 8570 0.2984899958831368 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7908 0.2754327756643928 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7660 0.2667950254918119 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5631 0.19612569041049513 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 5076 0.1767952414355662 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 4950 0.17240670707369046 No Hit GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA 4172 0.14530924887099728 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 3816 0.13290989781680865 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG 3720 0.12956625258871282 TruSeq Adapter, Index 27 (97% over 38bp) AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 3690 0.12852136345493287 No Hit GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA 3677 0.12806857816362827 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3606 0.12559567388034906 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 3578 0.12462044402215444 No Hit CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC 3129 0.10898193665324796 TruSeq Adapter, Index 27 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.006652460818398965 0.0 0.0 2 0.00734905357425226 0.0 0.0 3 0.008150135243483549 0.0 0.0 4 0.008707409448166184 0.0 0.0 5 0.01051855061338475 0.0 0.0 6 0.011702758298335352 0.0 0.0 7 0.013339751274590595 0.0 0.0 8 0.014314981132785207 0.0 0.0 9 0.01602163338462578 0.0 0.0 10-11 0.02492060584065162 0.0 0.0 12-13 0.03169497039132491 0.0 0.0 14-15 0.03873055722544319 0.0 0.0 16-17 0.04533077358715316 0.0 0.0 18-19 0.05334159027946605 0.0 0.0 20-21 0.0590710656963594 0.0 0.0 22-23 0.06478312629435641 0.0 0.0 24-25 0.07056484616793876 0.0 0.0 26-27 0.07690384024620375 0.0 0.0 28-29 0.08421806418266334 0.0 0.0 30-31 0.16373412726331693 0.0 0.0 32-33 0.17609864867971292 0.0 0.0 34-35 0.18449259138774513 0.0 0.0 36-37 0.1949763123633372 0.0 0.0 38-39 0.20697512258291023 0.0 0.0 40-41 0.22120302962121374 0.0 0.0 42-43 0.23776452239162582 0.0 0.0 44-45 0.2554231487525069 0.0 0.0 46-47 0.27337782703462554 0.0 0.0 48-49 0.2941537059779501 0.0 0.0 50-51 0.3159744740550545 0.0 0.0 52-53 0.3411563021791511 0.0 0.0 54-55 0.3684453233897039 0.0 0.0 56-57 0.398102759970158 0.0 0.0 58-59 0.43139989369994547 0.0 0.0 60-61 0.4652891312722083 0.0 0.0 62-63 0.5029747993638715 0.0 0.0 64-65 0.5442479201481791 0.0 0.0 66-67 0.5889517602550645 0.0 0.0 68-69 0.6368947566766674 0.0 0.0 70-71 0.6907065470663345 0.0 0.0 72-73 0.7496905386682122 0.0 0.0 74-75 0.8140905389468492 0.0 0.0 76-77 0.8874069265004085 0.0 0.0 78-79 0.9702143903524689 0.0 0.0 80-81 1.0597439742985135 0.0 0.0 82-83 1.1631705837238315 0.0 0.0 84-85 1.2842384046911341 0.0 0.0 86-87 1.4168696654056014 0.0 0.0 88-89 1.5674381895832912 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 47020 0.0 82.08896 3 CGGAAGA 47105 0.0 81.78958 4 GATCGGA 47315 0.0 81.76136 1 GAAGAGC 47225 0.0 81.48567 6 ATCGGAA 48030 0.0 80.362755 2 GGAAGAG 49470 0.0 78.03736 5 GAGCACA 55690 0.0 69.09115 9 AGAGCAC 55880 0.0 68.86473 8 AAGAGCA 56020 0.0 68.59937 7 CGTGGCA 120 0.0 59.457436 1 ATCGGAC 150 0.0 56.999603 2 ATTCGGA 70 1.2951205E-9 54.285336 2 TTCCCTA 615 0.0 54.054306 8 CGAGGCG 100 0.0 52.322544 1 TTGTGTT 23615 0.0 51.725327 1 ACCGGGC 80 4.1763997E-9 47.565952 1 GTGACCC 100 1.2732926E-11 47.565952 1 CGGCAGA 185 0.0 46.215897 4 GCCGTCT 42285 0.0 45.53022 50-51 ATGCCGT 42200 0.0 45.50786 48-49 >>END_MODULE