FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_50330665-1.341000000356c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_50330665-1.341000000356c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2871118
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT36262412.630062574927258TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC206330.7186399165760516No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA192230.6695301272883942No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC114880.4001228789621325No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT106570.3711794499564281TruSeq Adapter, Index 27 (97% over 39bp)
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT85700.2984899958831368No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC79080.2754327756643928No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT76600.2667950254918119No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT56310.19612569041049513No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC50760.1767952414355662No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT49500.17240670707369046No Hit
GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA41720.14530924887099728No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT38160.13290989781680865No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG37200.12956625258871282TruSeq Adapter, Index 27 (97% over 38bp)
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC36900.12852136345493287No Hit
GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA36770.12806857816362827No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36060.12559567388034906No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC35780.12462044402215444No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC31290.10898193665324796TruSeq Adapter, Index 27 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG470200.082.088963
CGGAAGA471050.081.789584
GATCGGA473150.081.761361
GAAGAGC472250.081.485676
ATCGGAA480300.080.3627552
GGAAGAG494700.078.037365
GAGCACA556900.069.091159
AGAGCAC558800.068.864738
AAGAGCA560200.068.599377
CGTGGCA1200.059.4574361
ATCGGAC1500.056.9996032
ATTCGGA701.2951205E-954.2853362
TTCCCTA6150.054.0543068
CGAGGCG1000.052.3225441
TTGTGTT236150.051.7253271
ACCGGGC804.1763997E-947.5659521
GTGACCC1001.2732926E-1147.5659521
CGGCAGA1850.046.2158974
GCCGTCT422850.045.5302250-51
ATGCCGT422000.045.5078648-49
Produced by FastQC (version 0.11.2)