FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_50220371-2.34100000035905.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_50220371-2.34100000035905.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3444146
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1879255.456359863954664No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1135703.2974792590093447No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT39370.11430990440010383TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG200.00207601371.23637
CGGACGG806.91216E-1153.5227931
CGGGACG451.5023577E-552.8620221
CGACGGG1450.049.2163621
CGCGGAG1550.042.9717061
GTCGCAC608.216628E-539.646511
CCGCGAG608.216628E-539.646511
CGGCGAG1101.5970727E-938.9256671
AATCCGG500.001603649938.0606541
TCGTGCG651.328537E-436.539393
GTTACGG1203.739842E-935.6818581
TCGACGC550.00258001734.546332
CGATGCG702.0350252E-433.9827271
AGTCGCC702.0350252E-433.9827271
CTGCGGA1308.169081E-932.9371031
GTTTGAT136450.031.7835123
GGCCGTC753.051041E-431.7172131
AGTACGC902.3881761E-531.7172131
CGAGGGG4350.031.7172091
AATGCGC600.003951547731.667472
Produced by FastQC (version 0.11.2)