##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_50110001-2.341000000358f7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6097714 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.675585145515186 33.0 33.0 33.0 33.0 33.0 2 32.71383554558315 33.0 33.0 33.0 33.0 33.0 3 32.74309044340223 33.0 33.0 33.0 33.0 33.0 4 36.66185065419598 37.0 37.0 37.0 37.0 37.0 5 36.656451745686994 37.0 37.0 37.0 37.0 37.0 6 36.54637131226555 37.0 37.0 37.0 37.0 37.0 7 36.49497779003738 37.0 37.0 37.0 37.0 37.0 8 36.42839037711509 37.0 37.0 37.0 37.0 37.0 9 36.41627354119921 37.0 37.0 37.0 37.0 37.0 10-11 36.36841019437776 37.0 37.0 37.0 37.0 37.0 12-13 36.37407272627086 37.0 37.0 37.0 37.0 37.0 14-15 38.26055551309884 40.0 37.0 40.0 37.0 40.0 16-17 38.24790987245384 40.0 37.0 40.0 37.0 40.0 18-19 38.24097850440345 40.0 37.0 40.0 37.0 40.0 20-21 38.170475197754435 40.0 37.0 40.0 37.0 40.0 22-23 38.07017170697084 40.0 37.0 40.0 37.0 40.0 24-25 38.046655271139315 40.0 37.0 40.0 35.0 40.0 26-27 37.95760739188489 40.0 37.0 40.0 33.0 40.0 28-29 37.854095485619695 40.0 37.0 40.0 33.0 40.0 30-31 37.79790508377402 40.0 37.0 40.0 33.0 40.0 32-33 37.67827418603103 40.0 37.0 40.0 33.0 40.0 34-35 37.57616780649273 40.0 37.0 40.0 33.0 40.0 36-37 37.480607568672454 40.0 37.0 40.0 33.0 40.0 38-39 37.40977495828764 40.0 37.0 40.0 33.0 40.0 40-41 37.29326326226517 40.0 37.0 40.0 33.0 40.0 42-43 37.14528452793949 40.0 37.0 40.0 33.0 40.0 44-45 37.04908708411054 40.0 37.0 40.0 33.0 40.0 46-47 36.86013873395833 38.5 37.0 40.0 33.0 40.0 48-49 36.77372462860672 37.0 37.0 40.0 33.0 40.0 50-51 36.62551982595445 37.0 37.0 40.0 33.0 40.0 52-53 36.4673061084859 37.0 37.0 40.0 33.0 40.0 54-55 36.323420301444116 37.0 37.0 40.0 33.0 40.0 56-57 36.11851351178491 37.0 37.0 40.0 33.0 40.0 58-59 35.83796706437855 37.0 37.0 40.0 27.0 40.0 60-61 35.59735566476224 37.0 37.0 40.0 27.0 40.0 62-63 35.28666119795058 37.0 33.0 38.5 27.0 40.0 64-65 34.99766838851412 37.0 33.0 37.0 27.0 40.0 66-67 34.734586190824956 37.0 33.0 37.0 27.0 40.0 68-69 34.462757354641425 37.0 33.0 37.0 27.0 40.0 70-71 34.180473616834114 37.0 33.0 37.0 27.0 40.0 72-73 33.91250975365522 37.0 33.0 37.0 27.0 37.0 74-75 33.59096253120431 37.0 33.0 37.0 27.0 37.0 76-77 30.740814426521155 33.0 30.0 35.0 24.5 37.0 78-79 33.25654917564189 35.0 33.0 37.0 27.0 37.0 80-81 33.66056640242556 37.0 33.0 37.0 27.0 37.0 82-83 33.56508799855159 37.0 33.0 37.0 27.0 37.0 84-85 33.45903128615085 37.0 33.0 37.0 27.0 37.0 86-87 33.377266054131105 37.0 33.0 37.0 27.0 37.0 88-89 33.11453767756244 37.0 33.0 37.0 27.0 37.0 90-91 33.1137648469574 37.0 33.0 37.0 27.0 37.0 92-93 32.974827697724095 37.0 33.0 37.0 27.0 37.0 94-95 32.805106635043884 37.0 33.0 37.0 27.0 37.0 96-97 32.71359627558787 33.0 33.0 37.0 27.0 37.0 98-99 32.583830514189415 33.0 33.0 37.0 27.0 37.0 100-101 30.92984748054763 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.13073462578749684 2306 2 0.12953284757607264 2306 3 0.13010466915960706 2306 4 0.20750964328272659 2306 5 0.14499327295394693 2306 6 0.13133803342186212 2306 7 0.20097403029350147 2306 8 0.24149187737057787 2306 9 0.17847739972046384 2306 10-11 0.183324788283322 2306 12-13 0.18729973233479313 2306 14-15 0.3736508760660726 2306 16-17 0.3592158188044081 2306 18-19 0.357029477090272 2306 20-21 0.37470055167552374 2306 22-23 0.34745159697463635 2306 24-25 0.40109773258441095 2306 26-27 0.3528732681039983 2306 28-29 0.3295940778222999 2306 30-31 0.3124349604657084 2306 32-33 0.439713186068289 2306 34-35 0.36432238238073467 2306 36-37 0.2885744575220599 2306 38-39 0.4127729123782089 2306 40-41 0.43959757987325077 2306 42-43 0.41475306957590874 2306 44-45 0.3798384098229022 2306 46-47 0.46734117380897544 2306 48-49 0.4524217959571146 2306 50-51 0.43546359058802153 2306 52-53 0.38410353201083325 2306 54-55 0.4243223538652501 2306 56-57 0.46796254579462726 2306 58-59 0.4436986901039788 2306 60-61 0.4662836814388598 2306 62-63 0.41784432711842356 2306 64-65 0.3098883841986648 2306 66-67 0.24152069674511267 2306 68-69 0.18640439838790002 2306 70-71 0.17911713489787218 2306 72-73 0.18118212726680127 2306 74-75 0.2772390046398314 2306 76-77 -0.21013551307360956 2306 78-79 0.09857809653652083 2306 80-81 0.21709167541265373 2306 82-83 0.27646058693912323 2306 84-85 0.34465687960746294 2306 86-87 0.22418834964434353 2306 88-89 0.6029392989171853 2306 90-91 0.37677923207628794 2306 92-93 0.39953701494201255 2306 94-95 0.30635956438417367 2306 96-97 0.34609750199989264 2306 98-99 0.3240649858822451 2306 100-101 0.13230244658806 2307 1 0.1694223526691374 2307 2 0.13706203023966168 2307 3 0.14071353986539492 2307 4 0.1946638443662181 2307 5 0.15645975824734393 2307 6 0.14535755613656676 2307 7 0.1558761790438794 2307 8 0.191768651186905 2307 9 0.1480518882018913 2307 10-11 0.18511746025386344 2307 12-13 0.1980143689042677 2307 14-15 0.3530605005219982 2307 16-17 0.36016969221179096 2307 18-19 0.31941485779191936 2307 20-21 0.3315745207554457 2307 22-23 0.3913995910675965 2307 24-25 0.3578645493567336 2307 26-27 0.40314770477203155 2307 28-29 0.38097351028347504 2307 30-31 0.34634193166411364 2307 32-33 0.297372833103438 2307 34-35 0.3163889562480904 2307 36-37 0.2801992469806507 2307 38-39 0.38632629680662234 2307 40-41 0.3078034105433005 2307 42-43 0.35217523065374934 2307 44-45 0.261039676319605 2307 46-47 0.4941511367156579 2307 48-49 0.3318848579224394 2307 50-51 0.28216745884574124 2307 52-53 0.20115423703736468 2307 54-55 0.19963396703631275 2307 56-57 0.230808938901383 2307 58-59 0.24749439719829525 2307 60-61 0.2969527317327092 2307 62-63 0.3371217997024871 2307 64-65 0.3063378003869488 2307 66-67 0.2512154057582663 2307 68-69 0.2472391802250158 2307 70-71 0.17295310432321997 2307 72-73 0.19707305215955984 2307 74-75 0.11901089579685475 2307 76-77 -0.2522446864202905 2307 78-79 0.09217341891245923 2307 80-81 0.26446983076092323 2307 82-83 0.3131866937571033 2307 84-85 0.26666931085965473 2307 86-87 0.25144722328146685 2307 88-89 0.44448450649557003 2307 90-91 0.2768716880784794 2307 92-93 0.20494347276005698 2307 94-95 0.29478493646610104 2307 96-97 0.3103753786333243 2307 98-99 0.22113994575459373 2307 100-101 0.1907217460456856 2201 1 0.13468668718656573 2201 2 0.1261822495359155 2201 3 0.10623311250127898 2201 4 0.16822639336898249 2201 5 0.16061598321034154 2201 6 0.10965588031573503 2201 7 0.16575443222295405 2201 8 0.21347998349275343 2201 9 0.1912757803560936 2201 10-11 0.19023743407208116 2201 12-13 0.21036332802217572 2201 14-15 0.3428998238177101 2201 16-17 0.3702328870941116 2201 18-19 0.3506999286329986 2201 20-21 0.3552739823225224 2201 22-23 0.306587835012202 2201 24-25 0.3356320999411082 2201 26-27 0.3043966194982275 2201 28-29 0.32139585819231087 2201 30-31 0.3582226447654264 2201 32-33 0.4308328031395021 2201 34-35 0.38906947449049767 2201 36-37 0.37655544551048337 2201 38-39 0.482674830099171 2201 40-41 0.38238524090293424 2201 42-43 0.37674964810999967 2201 44-45 0.40123596162183617 2201 46-47 0.42631240607133947 2201 48-49 0.37668431341445086 2201 50-51 0.3613858055578518 2201 52-53 0.3661351386939913 2201 54-55 0.4396440680098621 2201 56-57 0.40885288080250604 2201 58-59 0.46836842373696896 2201 60-61 0.5004232417080843 2201 62-63 0.4280587230838293 2201 64-65 0.5306441154569583 2201 66-67 0.39288039337295544 2201 68-69 0.4645711053764927 2201 70-71 0.356362593296609 2201 72-73 0.3568562325912481 2201 74-75 0.35398981591821865 2201 76-77 0.031229785673389188 2201 78-79 0.2730674007775562 2201 80-81 0.4314556204584292 2201 82-83 0.5151510084115642 2201 84-85 0.5000201298212161 2201 86-87 0.3883250559675204 2201 88-89 0.6143241925807672 2201 90-91 0.3936907374405152 2201 92-93 0.47404731511021225 2201 94-95 0.5745642880879629 2201 96-97 0.4718525765044248 2201 98-99 0.47765415958024704 2201 100-101 0.4032048849041736 1101 1 0.1215356342485876 1101 2 0.12271311883147007 1101 3 0.1049829936779787 1101 4 0.13419663160428286 1101 5 0.1466444442262329 1101 6 0.14961223829043746 1101 7 0.190591326800714 1101 8 0.15799742327046573 1101 9 0.15330342109831463 1101 10-11 0.14487190811193784 1101 12-13 0.1972468708229016 1101 14-15 0.26177980366086473 1101 16-17 0.27200872522632125 1101 18-19 0.26544738483742236 1101 20-21 0.2758682457926227 1101 22-23 0.33451111971555036 1101 24-25 0.35087403947378704 1101 26-27 0.3442359168764 1101 28-29 0.28680383603336423 1101 30-31 0.33295815620172675 1101 32-33 0.3454981342846466 1101 34-35 0.33937833437210685 1101 36-37 0.368828193132515 1101 38-39 0.40347717769931535 1101 40-41 0.4237816226091624 1101 42-43 0.37289081095666887 1101 44-45 0.35989885352316264 1101 46-47 0.46323992729791996 1101 48-49 0.40002407686879593 1101 50-51 0.41134742531291835 1101 52-53 0.25357472782319235 1101 54-55 0.26286207315288124 1101 56-57 0.3491020686627593 1101 58-59 0.43640474327366263 1101 60-61 0.3755849114815035 1101 62-63 0.3931535340967969 1101 64-65 0.32031672041456005 1101 66-67 0.40879634454424263 1101 68-69 0.3677539643071768 1101 70-71 0.20932577671960217 1101 72-73 0.3150153943966032 1101 74-75 0.20699950926618982 1101 76-77 -0.11326563515903132 1101 78-79 0.18430652202115994 1101 80-81 0.30163987876444054 1101 82-83 0.47223080015923813 1101 84-85 0.41284678983689815 1101 86-87 0.3878167810660855 1101 88-89 0.5722413753518651 1101 90-91 0.4469964632752479 1101 92-93 0.4415981050101436 1101 94-95 0.5650522226488803 1101 96-97 0.48218073184604293 1101 98-99 0.38273029101806344 1101 100-101 0.3696130597434326 2304 1 0.11784167295091663 2304 2 0.08902731901569894 2304 3 0.07971197748383929 2304 4 0.08883119064257272 2304 5 0.11528537404664263 2304 6 0.05288014436160893 2304 7 0.09980401271951678 2304 8 0.052053914281074753 2304 9 0.11229021574646225 2304 10-11 0.14319361876580672 2304 12-13 0.11220949057008767 2304 14-15 0.25401792346239915 2304 16-17 0.2634356894947061 2304 18-19 0.24574902531117715 2304 20-21 0.25624449245349723 2304 22-23 0.1970670440973663 2304 24-25 0.22052825558761668 2304 26-27 0.1693669949356078 2304 28-29 0.2156277369130848 2304 30-31 0.291961857803301 2304 32-33 0.15782153321317338 2304 34-35 0.22092263970547776 2304 36-37 0.2595696163711949 2304 38-39 0.3575337609984359 2304 40-41 0.26317278922147835 2304 42-43 0.24960398271399242 2304 44-45 0.2815393416446881 2304 46-47 0.3897295528206257 2304 48-49 0.2995905515677819 2304 50-51 0.3484934430672695 2304 52-53 0.23722277255861712 2304 54-55 0.37085796147258776 2304 56-57 0.22629434641367396 2304 58-59 0.4692543675345817 2304 60-61 0.34273432272993887 2304 62-63 0.35924754674319814 2304 64-65 0.27997975537685704 2304 66-67 0.327990335986982 2304 68-69 0.3128148380388609 2304 70-71 0.29016264897709476 2304 72-73 0.12632566210667306 2304 74-75 0.20002518294776195 2304 76-77 0.03827433866582908 2304 78-79 0.18771153327241308 2304 80-81 0.2910881243105976 2304 82-83 0.42451064042143116 2304 84-85 0.39158398868226385 2304 86-87 0.2649147089392869 2304 88-89 0.5479684367576922 2304 90-91 0.3173848836990629 2304 92-93 0.40448622644186116 2304 94-95 0.37488281043670213 2304 96-97 0.30047476275346696 2304 98-99 0.3345608677615175 2304 100-101 0.4749498663020937 2202 1 0.07520843486700102 2202 2 0.08061036141952371 2202 3 0.02978179115615376 2202 4 0.06965289960472631 2202 5 0.07264533915496685 2202 6 0.06760286649775793 2202 7 0.06339895100084192 2202 8 0.01412960917885897 2202 9 0.054166111683358054 2202 10-11 0.15037465529572103 2202 12-13 0.09128055098607746 2202 14-15 0.10332192511956606 2202 16-17 0.06103736790886671 2202 18-19 0.011888385175275573 2202 20-21 0.15689832444665797 2202 22-23 0.24641181614446594 2202 24-25 0.16905812175429702 2202 26-27 0.09009122512654955 2202 28-29 0.1597296919573168 2202 30-31 0.2212713450071604 2202 32-33 0.24522795816235288 2202 34-35 0.2923499264626983 2202 36-37 0.2597289753715799 2202 38-39 0.21854014027307045 2202 40-41 0.21079894137189115 2202 42-43 0.3180156827179559 2202 44-45 0.3409005170030639 2202 46-47 0.3612675020647771 2202 48-49 0.4069897865400165 2202 50-51 0.26410987222732984 2202 52-53 0.23028919211426313 2202 54-55 0.08992649975006373 2202 56-57 0.3093789427245994 2202 58-59 0.38327129869640686 2202 60-61 0.20689264317809375 2202 62-63 0.28823376731341455 2202 64-65 0.1887935210666356 2202 66-67 0.17716901496569903 2202 68-69 0.1396701338971056 2202 70-71 -0.024796664808825142 2202 72-73 0.06104347381063491 2202 74-75 0.10352055113241221 2202 76-77 -0.257931850549145 2202 78-79 0.18031676328004664 2202 80-81 0.32988566236043226 2202 82-83 0.40687266287118007 2202 84-85 0.40485585377754774 2202 86-87 0.3874527352487931 2202 88-89 0.5520478284914958 2202 90-91 0.2693623521767776 2202 92-93 0.40754965226510365 2202 94-95 0.43985297297347614 2202 96-97 0.34857854227004736 2202 98-99 0.3453876596902177 2202 100-101 0.37514585486797714 1102 1 0.10709389231930544 1102 2 0.08354830050375739 1102 3 0.0823155779159066 1102 4 0.09368034136265635 1102 5 0.08525436473416903 1102 6 0.032666652998798895 1102 7 0.06551799892599064 1102 8 0.10846198528216888 1102 9 0.09206924622679225 1102 10-11 0.10839563499708049 1102 12-13 0.12957467217162133 1102 14-15 0.1664859806322383 1102 16-17 0.1458850562801004 1102 18-19 0.017686507252172134 1102 20-21 0.0664961941932205 1102 22-23 0.09258128506194652 1102 24-25 0.05481710707459797 1102 26-27 0.09943032056877854 1102 28-29 0.10764068146905714 1102 30-31 0.12140089087762362 1102 32-33 0.09594561085847886 1102 34-35 0.06111137302756475 1102 36-37 0.07715829137033836 1102 38-39 0.13490863081924687 1102 40-41 0.15946776311553634 1102 42-43 0.20787655184980025 1102 44-45 0.03801473274978662 1102 46-47 0.16995550332690357 1102 48-49 0.05700688843605661 1102 50-51 0.10580965943371012 1102 52-53 0.049622841931871164 1102 54-55 0.0389001082310827 1102 56-57 0.08548285622566709 1102 58-59 0.18942669565006298 1102 60-61 0.052434143170714265 1102 62-63 0.08145602006245412 1102 64-65 -0.005127044554569693 1102 66-67 0.1480269555863316 1102 68-69 0.14072924519211938 1102 70-71 0.019884232857393158 1102 72-73 0.05481371733687013 1102 74-75 0.055520568273983884 1102 76-77 0.007353078149044734 1102 78-79 0.003837227067599258 1102 80-81 0.024084873816207164 1102 82-83 0.1739857088640946 1102 84-85 0.25352658014070784 1102 86-87 0.23667608696329268 1102 88-89 0.4814489796117911 1102 90-91 0.262255343827718 1102 92-93 0.2375403307397761 1102 94-95 0.3126871467995258 1102 96-97 0.17532370148116172 1102 98-99 0.15068631117198095 1102 100-101 0.20749118270139988 2305 1 0.029160577273053434 2305 2 0.0762243821060764 2305 3 0.04729907586209947 2305 4 0.09459583116236558 2305 5 0.06302059175374097 2305 6 0.03559063522654782 2305 7 0.011278598882078938 2305 8 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-0.7173292596478476 1213 76-77 -0.18190885271484092 1213 78-79 -0.6960092007473548 1213 80-81 -0.7233004028253447 1213 82-83 -1.2181154418812383 1213 84-85 -1.2462484268225396 1213 86-87 -1.0250815370053559 1213 88-89 -0.7214341552237045 1213 90-91 -0.7258178656304182 1213 92-93 -0.9433679924792955 1213 94-95 -0.8325721832964135 1213 96-97 -0.8635505678361923 1213 98-99 -0.9039369308198495 1213 100-101 -0.8808045420452579 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 13.0 8 79.0 9 282.0 10 427.0 11 297.0 12 285.0 13 585.0 14 1421.0 15 3022.0 16 5357.0 17 7876.0 18 10457.0 19 12624.0 20 15325.0 21 18384.0 22 22283.0 23 27085.0 24 32965.0 25 39188.0 26 48158.0 27 59334.0 28 74501.0 29 94365.0 30 110555.0 31 134449.0 32 176494.0 33 244339.0 34 363930.0 35 629107.0 36 1263798.0 37 2138320.0 38 562169.0 39 239.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.08501988046261 28.99502023363753 16.47475579597003 16.44520408992983 2 33.408159188837 23.422941777853143 28.451071991897294 14.717827041412567 3 39.22614667547219 20.58222675362633 21.841768682024494 18.34985788887699 4 33.27544695859508 20.556552314421598 27.297862714974315 18.870138012009 5 35.05664581841654 18.539570730932937 26.96981196559891 19.43397148505161 6 32.10562187731337 19.527941126789482 28.70890632128696 19.65753067461019 7 35.918509789078335 20.02453706421784 22.24025265861928 21.81670048808455 8 31.1015406757352 25.969961857837216 22.193054643100677 20.735442823326906 9 29.402920504307023 22.574722264770045 26.866100968330098 21.156256262592834 10-11 22.553739656935377 30.137412805023544 31.44673757335776 15.862109964683318 12-13 22.466001193234057 29.322283727967562 31.574496606433165 16.637218472365216 14-15 22.387119172857236 29.04135057826589 31.81265962949394 16.758870619382936 16-17 22.10799406754137 28.924761963254475 31.84083253186212 17.126411437342036 18-19 21.024485569510148 29.259547102405918 31.9172152055672 17.798752122516735 20-21 21.595297844405298 29.054306253130267 31.21013875035792 18.14025715210651 22-23 20.98265545683224 28.543439010384517 31.531648545434848 18.942256987348387 24-25 20.505971582137175 27.81118465051001 32.18494668657795 19.497897080774862 26-27 20.51522095001504 28.234400629481804 31.652165057265723 19.598213363237434 28-29 20.685809468925566 28.76700186332124 30.977461389628967 19.569727278124226 30-31 19.739520416995614 30.460226570154 30.519240489140685 19.281012523709705 32-33 19.38951220080181 31.153494571900225 31.030948647312744 18.42604457998522 34-35 19.98645721986961 30.288957468323375 32.18306073390782 17.541524577899192 36-37 19.635150145326264 29.967763038241397 33.15411701441487 17.242969802017463 38-39 20.158680451946882 29.469386101742085 31.758008620843047 18.613924825467983 40-41 20.342887329042814 29.18297998170203 31.337391294792788 19.136741394462376 42-43 20.40566124687291 28.824592137860506 31.35800486951593 19.411741745750653 44-45 20.00704862171402 28.33527889287112 31.810267437630852 19.847405047784015 46-47 20.71204191401107 28.706541569276006 30.96409704013293 19.617319476579993 48-49 20.443757613578914 29.822768388522853 30.397683544911043 19.33579045298719 50-51 21.432880058018192 29.625669834903345 28.898556400615476 20.042893706462987 52-53 21.08995598927054 29.55606005244027 28.7789187359494 20.57506522233979 54-55 21.725378923780607 29.40126249838549 29.10919442434133 19.764164153492576 56-57 21.21442902108492 30.10786983906745 29.01139913234684 19.666302007500786 58-59 20.82126046042263 31.085949997309847 28.705076118151435 19.387713424116086 60-61 20.96038204884139 31.017899477638384 28.493814561749538 19.52790391177069 62-63 20.401613762754963 30.766401191142624 29.24604289717334 19.58594214892907 64-65 20.803831692372896 29.686438998851205 29.86541707862898 19.644312230146916 66-67 21.063986107455268 29.623410555423725 29.33092297802521 19.981680359095805 68-69 21.23900867233734 30.345545181498075 29.426634348728314 18.988811797436274 70-71 20.54411877373579 30.93601678224667 29.580727649578236 18.9391367944393 72-73 20.31864078967928 30.32580545014532 29.65398421970204 19.70156954047336 74-75 20.603683162674372 29.349297756806408 29.357434333845234 20.68958474667399 76-77 20.583513331808692 28.703205454847918 28.932426663814255 21.78085454952914 78-79 19.907661377922988 29.2819358305953 29.32504938540016 21.485353406081543 80-81 19.444733978940132 29.86355558183463 28.971880455339033 21.71982998388621 82-83 18.65952884892603 30.118404398275928 28.98537177036051 22.23669498243753 84-85 18.353405971020972 30.47822439798693 30.000650312949784 21.167719318042312 86-87 18.80289061117274 30.161888266684734 30.670981543779657 20.364239578362866 88-89 19.60234956987157 30.002214408912746 30.05872284376033 20.336713177455348 90-91 19.541894199491814 30.160157409792017 29.49552097293556 20.802427417780613 92-93 18.93392160684508 30.56012478171076 29.738664873758108 20.76728873768606 94-95 19.1011664490111 30.071909177508026 29.77338668177597 21.053537691704904 96-97 19.419225736569132 30.665564185786675 29.395950441510454 20.51925963613374 98-99 20.194023571336643 30.47279249524629 28.747756642957633 20.58542729045944 100-101 19.886856406624755 30.970171868201984 27.957908221322185 21.185063503851072 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 12211.0 1 6927.5 2 1773.5 3 1902.5 4 1529.0 5 1097.0 6 970.5 7 917.0 8 941.5 9 938.5 10 883.0 11 827.5 12 807.0 13 781.5 14 794.5 15 827.0 16 877.0 17 966.5 18 1068.0 19 1257.5 20 1610.5 21 2088.5 22 2782.0 23 3845.5 24 5467.5 25 7752.0 26 10475.0 27 14159.5 28 19209.0 29 25015.0 30 32513.5 31 41955.0 32 59366.0 33 94762.0 34 143678.0 35 188031.5 36 246464.0 37 334133.5 38 415829.5 39 459281.5 40 462009.0 41 450584.5 42 451592.5 43 446228.0 44 396773.0 45 328007.5 46 281991.5 47 245926.5 48 208304.5 49 167807.0 50 123210.0 51 92688.5 52 75107.0 53 57453.5 54 41433.5 55 30334.0 56 22968.5 57 17518.5 58 13133.5 59 9804.0 60 7352.5 61 5851.0 62 4729.5 63 3669.5 64 2735.5 65 2022.5 66 1578.0 67 1285.0 68 1066.5 69 878.5 70 743.5 71 579.5 72 422.0 73 318.0 74 236.0 75 193.0 76 143.0 77 116.0 78 92.5 79 59.5 80 45.5 81 35.0 82 26.5 83 16.0 84 6.0 85 8.0 86 8.0 87 3.5 88 3.0 89 2.0 90 0.0 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16529145184572447 2 0.0 3 3.4439135715450084E-4 4 0.021122669905476052 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.755880646419297E-4 12-13 0.0 14-15 0.0 16-17 0.001205369750040753 18-19 0.0 20-21 0.0 22-23 5.329866241676799E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.019773639760737E-4 38-39 8.281792160143949E-4 40-41 0.005206869328407335 42-43 0.003083122625954579 44-45 0.0011151720136431456 46-47 0.004559085585188154 48-49 0.007650408005360697 50-51 0.0051822699457534415 52-53 0.0018039547279521473 54-55 0.011094321576905706 56-57 0.018916925260843653 58-59 0.022992222987171917 60-61 0.011356714991880564 62-63 6.477837432191802E-4 64-65 0.014398838646745321 66-67 0.03395534785659019 68-69 0.01972870488842212 70-71 0.03648908426994116 72-73 0.029871850336043965 74-75 0.02926506556391461 76-77 0.03758785669514838 78-79 0.016784978764172936 80-81 0.012504686182395567 82-83 8.527785986682879E-4 84-85 0.010651532689135632 86-87 0.021106270317040123 88-89 0.020975073609552697 90-91 0.024992972776355205 92-93 0.042474934049055106 94-95 0.02773170404515528 96-97 0.028953473383631967 98-99 0.0363660873566717 100-101 0.016998173413840006 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6097714.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.807048861505756 #Duplication Level Percentage of deduplicated Percentage of total 1 79.99740232793691 28.64470893949972 2 9.352214792634529 6.697504240863231 3 3.404278966437626 3.6569154986828516 4 1.6169733765640828 2.3159617880953625 5 1.093038238710968 1.9569236810508914 6 0.7384044894453577 1.5864051379875084 7 0.5809735276606053 1.4562063244529264 8 0.39015575092873156 1.117626082968205 9 0.330051762704778 1.063636163459648 >10 2.0315932880171137 13.981930223103687 >50 0.22784734255624906 5.774935838732588 >100 0.19490407967958334 14.615338452171814 >500 0.026724778775351548 6.569081031502925 >1k 0.01516350782797025 9.13599084612934 >5k 1.3688667618293624E-4 0.3682820849340925 >10k+ 1.3688344398983245E-4 1.0585536663650859 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 28402 0.46578111075724443 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 19839 0.3253514349803877 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16550 0.27141318861461855 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8267 0.13557539759982185 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7777 0.1275395992662168 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6497 0.10654812606822818 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.231843605652873E-4 0.0 0.0 2 6.887827143090017E-4 0.0 0.0 3 7.871802449245734E-4 0.0 0.0 4 9.347765408479309E-4 0.0 0.0 5 0.001016774483027574 0.0 0.0 6 0.0012299691326946459 0.0 0.0 7 0.0013119670748742889 0.0 0.0 8 0.0015907600782850753 0.0 0.0 9 0.0018039547279521473 0.0 0.0 10-11 0.0026239341497485778 0.0 0.0 12-13 0.004419689083482761 0.0 0.0 14-15 0.007068222615885231 0.0 0.0 16-17 0.013857652228359676 0.0 0.0 18-19 0.02155725899902816 0.0 0.0 20-21 0.02854348367273375 0.0 0.0 22-23 0.03644808529885134 0.0 8.199794217964306E-6 24-25 0.04591884762060011 0.0 1.6399588435928612E-5 26-27 0.056283387512107 0.0 1.6399588435928612E-5 28-29 0.06754170497337199 0.0 1.6399588435928612E-5 30-31 0.08074337366429452 0.0 1.6399588435928612E-5 32-33 0.09496181683824463 0.0 1.6399588435928612E-5 34-35 0.11077921988469777 0.0 1.6399588435928612E-5 36-37 0.1272444066743701 0.0 1.6399588435928612E-5 38-39 0.14460337103380055 0.0 1.6399588435928612E-5 40-41 0.16265931790175792 0.0 1.6399588435928612E-5 42-43 0.1822404264942567 0.0 1.6399588435928612E-5 44-45 0.20205932911907643 0.0 1.6399588435928612E-5 46-47 0.22355098976436089 0.0 1.6399588435928612E-5 48-49 0.24545264012054352 0.0 1.6399588435928612E-5 50-51 0.27007662215709033 0.0 1.6399588435928612E-5 52-53 0.29550418402699763 0.0 1.6399588435928612E-5 54-55 0.32115314034079 0.0 1.6399588435928612E-5 56-57 0.34963102565978005 0.0 1.6399588435928612E-5 58-59 0.37990466591250427 0.0 1.6399588435928612E-5 60-61 0.41271204257857946 0.0 1.6399588435928612E-5 62-63 0.4494963194403673 0.0 1.6399588435928612E-5 64-65 0.4877565592613888 0.0 1.6399588435928612E-5 66-67 0.5312892667645612 0.0 1.6399588435928612E-5 68-69 0.5818409981183112 0.0 1.6399588435928612E-5 70-71 0.6375831992120327 0.0 1.6399588435928612E-5 72-73 0.7006806157192679 0.0 1.6399588435928612E-5 74-75 0.7758727286980005 0.0 1.6399588435928612E-5 76-77 0.8669478430769302 0.0 1.6399588435928612E-5 78-79 0.975652515024483 0.0 1.6399588435928612E-5 80-81 1.1100553420511359 0.0 1.6399588435928612E-5 82-83 1.281603236885167 0.0 1.6399588435928612E-5 84-85 1.4917721624858102 0.0 1.6399588435928612E-5 86-87 1.7383809735910867 0.0 1.6399588435928612E-5 88-89 2.0333275716112627 0.0 1.6399588435928612E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGACGG 60 2.3228495E-8 55.508926 1 CGACGGG 305 0.0 51.479008 1 CGCGGAG 445 0.0 51.321255 1 CGAGTGG 305 0.0 48.359066 1 ACGAGCG 100 1.2732926E-11 47.579082 1 CGGAGCG 105 2.0008883E-11 45.31341 1 GGACGGG 855 0.0 45.07492 1 CACGGGG 600 0.0 42.821175 1 CGAGGGG 1045 0.0 42.79841 1 CGCGGGG 455 0.0 40.78207 1 CGAGCGG 150 0.0 38.063267 1 ATTACGG 285 0.0 36.727715 1 TGTACCG 195 0.0 36.539257 2 CAACGGG 145 1.6370905E-11 36.094475 1 CGAGTCG 55 0.0025595278 34.60297 1 CGTCCGC 55 0.0025802176 34.546207 3 CCGGGGC 180 0.0 34.362667 1 GTTACGG 280 0.0 33.98506 1 ATACGGG 690 0.0 33.788044 1 CGGGTGG 835 0.0 33.61875 1 >>END_MODULE