FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_50110001-2.341000000358f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_50110001-2.341000000358f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6097714
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT284020.46578111075724443TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC198390.3253514349803877No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165500.27141318861461855No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82670.13557539759982185No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG77770.1275395992662168No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA64970.10654812606822818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGG602.3228495E-855.5089261
CGACGGG3050.051.4790081
CGCGGAG4450.051.3212551
CGAGTGG3050.048.3590661
ACGAGCG1001.2732926E-1147.5790821
CGGAGCG1052.0008883E-1145.313411
GGACGGG8550.045.074921
CACGGGG6000.042.8211751
CGAGGGG10450.042.798411
CGCGGGG4550.040.782071
CGAGCGG1500.038.0632671
ATTACGG2850.036.7277151
TGTACCG1950.036.5392572
CAACGGG1451.6370905E-1136.0944751
CGAGTCG550.002559527834.602971
CGTCCGC550.002580217634.5462073
CCGGGGC1800.034.3626671
GTTACGG2800.033.985061
ATACGGG6900.033.7880441
CGGGTGG8350.033.618751
Produced by FastQC (version 0.11.2)