##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_44065-1.341000000357de.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3956237 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67114280565092 33.0 33.0 33.0 33.0 33.0 2 32.71993336091847 33.0 33.0 33.0 33.0 33.0 3 32.743905888347946 33.0 33.0 33.0 33.0 33.0 4 36.67584399013507 37.0 37.0 37.0 37.0 37.0 5 36.67613062614803 37.0 37.0 37.0 37.0 37.0 6 36.57030000983258 37.0 37.0 37.0 37.0 37.0 7 36.53456276759962 37.0 37.0 37.0 37.0 37.0 8 36.484591292179914 37.0 37.0 37.0 37.0 37.0 9 36.48705044717998 37.0 37.0 37.0 37.0 37.0 10-11 36.453238645713085 37.0 37.0 37.0 37.0 37.0 12-13 36.43865559621428 37.0 37.0 37.0 37.0 37.0 14-15 38.38273667629113 40.0 37.0 40.0 37.0 40.0 16-17 38.359693820162946 40.0 37.0 40.0 37.0 40.0 18-19 38.35770442973967 40.0 37.0 40.0 37.0 40.0 20-21 38.30343177115021 40.0 37.0 40.0 37.0 40.0 22-23 38.22347978647386 40.0 37.0 40.0 37.0 40.0 24-25 38.19135393051529 40.0 37.0 40.0 37.0 40.0 26-27 38.11192062052905 40.0 37.0 40.0 37.0 40.0 28-29 38.01693806513614 40.0 37.0 40.0 37.0 40.0 30-31 37.967097522216186 40.0 37.0 40.0 35.0 40.0 32-33 37.86897890596544 40.0 37.0 40.0 33.0 40.0 34-35 37.79753399505641 40.0 37.0 40.0 33.0 40.0 36-37 37.72369261497731 40.0 37.0 40.0 33.0 40.0 38-39 37.6339516565868 40.0 37.0 40.0 33.0 40.0 40-41 37.55380112465456 40.0 37.0 40.0 33.0 40.0 42-43 37.425224272458905 40.0 37.0 40.0 33.0 40.0 44-45 37.3445373722555 40.0 37.0 40.0 33.0 40.0 46-47 37.16799208439737 40.0 37.0 40.0 33.0 40.0 48-49 37.097305217053474 40.0 37.0 40.0 33.0 40.0 50-51 36.95373507704417 37.0 37.0 40.0 33.0 40.0 52-53 36.802420582993385 37.0 37.0 40.0 33.0 40.0 54-55 36.66833533986968 37.0 37.0 40.0 33.0 40.0 56-57 36.47173842214205 37.0 37.0 40.0 33.0 40.0 58-59 36.202288689984954 37.0 37.0 40.0 33.0 40.0 60-61 35.96481087963133 37.0 37.0 40.0 33.0 40.0 62-63 35.697153507234276 37.0 37.0 40.0 30.0 40.0 64-65 35.42735811833315 37.0 33.0 38.5 27.0 40.0 66-67 35.16834507133925 37.0 33.0 37.0 27.0 40.0 68-69 34.870133412128745 37.0 33.0 37.0 27.0 40.0 70-71 34.54523705227973 37.0 33.0 37.0 27.0 40.0 72-73 34.26565205775084 37.0 33.0 37.0 27.0 38.5 74-75 33.91056349758622 37.0 33.0 37.0 27.0 37.0 76-77 31.10813330950598 33.0 30.0 35.0 24.5 37.0 78-79 33.58189815220878 35.0 33.0 37.0 27.0 37.0 80-81 33.973564020557916 37.0 33.0 37.0 27.0 37.0 82-83 33.87240792197232 37.0 33.0 37.0 27.0 37.0 84-85 33.74445514765672 37.0 33.0 37.0 27.0 37.0 86-87 33.65344707609782 37.0 33.0 37.0 27.0 37.0 88-89 33.38263114671847 37.0 33.0 37.0 27.0 37.0 90-91 33.3833374997504 37.0 33.0 37.0 27.0 37.0 92-93 33.2457749624201 37.0 33.0 37.0 27.0 37.0 94-95 33.069985190472664 37.0 33.0 37.0 27.0 37.0 96-97 32.9666080419348 33.0 33.0 37.0 27.0 37.0 98-99 32.84862307288466 33.0 33.0 37.0 27.0 37.0 100-101 31.1979364481956 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.16132839179464042 2306 2 0.15164837204019221 2306 3 0.14875151287099442 2306 4 0.21034341989057026 2306 5 0.17675102458699143 2306 6 0.20228320802461752 2306 7 0.24174061168994143 2306 8 0.22972355943607425 2306 9 0.1893332837788435 2306 10-11 0.22584441779093822 2306 12-13 0.25954217829585247 2306 14-15 0.4135512356829807 2306 16-17 0.39525215315160267 2306 18-19 0.4113333196523925 2306 20-21 0.42179954577780876 2306 22-23 0.40542400807441936 2306 24-25 0.4284427264691715 2306 26-27 0.42841011322212097 2306 28-29 0.3626467930768982 2306 30-31 0.4499082543408903 2306 32-33 0.4594580964697812 2306 34-35 0.5032761581064022 2306 36-37 0.402911090399769 2306 38-39 0.5634046776346793 2306 40-41 0.4963041002700592 2306 42-43 0.5991502460636795 2306 44-45 0.4654037887682563 2306 46-47 0.5764978552661546 2306 48-49 0.572555742295684 2306 50-51 0.47187362889527407 2306 52-53 0.5135257916691174 2306 54-55 0.57640360005351 2306 56-57 0.5977809876093048 2306 58-59 0.5623540740187138 2306 60-61 0.5139334618555793 2306 62-63 0.45160243162764147 2306 64-65 0.3820989264664334 2306 66-67 0.42270723144133626 2306 68-69 0.4519015707707794 2306 70-71 0.45266329445480835 2306 72-73 0.41903949213790526 2306 74-75 0.4066876886279829 2306 76-77 -0.07219427892342978 2306 78-79 0.336724434864891 2306 80-81 0.4499169067965383 2306 82-83 0.5585623630936496 2306 84-85 0.5151177220036018 2306 86-87 0.4153983891596411 2306 88-89 0.6780548488478715 2306 90-91 0.4870895768600647 2306 92-93 0.46532943245787806 2306 94-95 0.49464420222488314 2306 96-97 0.5119047532891443 2306 98-99 0.4876186381576346 2306 100-101 0.3656220921711544 2307 1 0.18575541849117627 2307 2 0.13449214510433194 2307 3 0.13369000121718244 2307 4 0.1779075409311801 2307 5 0.14510331145795874 2307 6 0.12550707812737727 2307 7 0.20883853684529896 2307 8 0.15398441514025762 2307 9 0.21198287042577135 2307 10-11 0.19277109511351398 2307 12-13 0.2074723013191644 2307 14-15 0.43613154802330456 2307 16-17 0.42017916674661393 2307 18-19 0.3502060863884964 2307 20-21 0.34426423717140864 2307 22-23 0.4108776264969123 2307 24-25 0.3433569005709529 2307 26-27 0.3556011792931031 2307 28-29 0.389068308399132 2307 30-31 0.40915596484962435 2307 32-33 0.42468392306675895 2307 34-35 0.4308993561010013 2307 36-37 0.4107244024212875 2307 38-39 0.4538817259487331 2307 40-41 0.37040631835802884 2307 42-43 0.39162112792724457 2307 44-45 0.4004951828140477 2307 46-47 0.6144340268262383 2307 48-49 0.45285569076302323 2307 50-51 0.486965492412331 2307 52-53 0.3990034623055436 2307 54-55 0.36364287973174214 2307 56-57 0.40054966710672346 2307 58-59 0.602881235660945 2307 60-61 0.43966332590543544 2307 62-63 0.4264495259280423 2307 64-65 0.3894272091623776 2307 66-67 0.3341653327685208 2307 68-69 0.33938972480976304 2307 70-71 0.2891704719062034 2307 72-73 0.3891520233086183 2307 74-75 0.26031773871947905 2307 76-77 -0.16378823213352334 2307 78-79 0.15656461811153832 2307 80-81 0.26053525504377006 2307 82-83 0.4162883862134734 2307 84-85 0.35390085248167935 2307 86-87 0.36715268318403815 2307 88-89 0.5576737364411599 2307 90-91 0.3303794677243701 2307 92-93 0.26266501925329067 2307 94-95 0.2985147744557608 2307 96-97 0.4817971242718855 2307 98-99 0.38467494413935555 2307 100-101 0.20855371095175101 2201 1 0.14276397966002463 2201 2 0.113686371669246 2201 3 0.13502805272078433 2201 4 0.20179433182500617 2201 5 0.14491961393348163 2201 6 0.16777321210283702 2201 7 0.15786252649837706 2201 8 0.2303651713122008 2201 9 0.19182009102215147 2201 10-11 0.20476470964844395 2201 12-13 0.21492754362283506 2201 14-15 0.33529409598700965 2201 16-17 0.35735835477615296 2201 18-19 0.3717572944711094 2201 20-21 0.35579813363303714 2201 22-23 0.41207835277137406 2201 24-25 0.3593148193992022 2201 26-27 0.38517031532665413 2201 28-29 0.32871213122998455 2201 30-31 0.3914362676691425 2201 32-33 0.43956700586322484 2201 34-35 0.3911037588553441 2201 36-37 0.35583183016692743 2201 38-39 0.5133094032953593 2201 40-41 0.4110499489546342 2201 42-43 0.4149507135359798 2201 44-45 0.45270106020738154 2201 46-47 0.4862086755982631 2201 48-49 0.34882150131280554 2201 50-51 0.33342197454255995 2201 52-53 0.39053877944567006 2201 54-55 0.49219241546074954 2201 56-57 0.45283074139261714 2201 58-59 0.5395158126992357 2201 60-61 0.37452901065982047 2201 62-63 0.4352909836875227 2201 64-65 0.4876716067567486 2201 66-67 0.4377187258293702 2201 68-69 0.45758964926507417 2201 70-71 0.29603040986850715 2201 72-73 0.384387533441803 2201 74-75 0.3506201429965259 2201 76-77 0.05260671396079175 2201 78-79 0.21423562859757084 2201 80-81 0.32998914959991055 2201 82-83 0.4187823198741114 2201 84-85 0.45893536611639973 2201 86-87 0.3923650893749908 2201 88-89 0.6825182311644156 2201 90-91 0.24332217559661018 2201 92-93 0.38696427063582206 2201 94-95 0.4146333732873515 2201 96-97 0.39266398688258164 2201 98-99 0.32888506797024775 2201 100-101 0.38034356772880784 1101 1 0.17371951054369816 1101 2 0.127658627118727 1101 3 0.14751163898399255 1101 4 0.15526608819622822 1101 5 0.17236373996194487 1101 6 0.15396181538735476 1101 7 0.2090910003913038 1101 8 0.18223927718879906 1101 9 0.19210299720715085 1101 10-11 0.21776513444325474 1101 12-13 0.20217605304212327 1101 14-15 0.30062134430479404 1101 16-17 0.37966148599497274 1101 18-19 0.371746841556984 1101 20-21 0.3933209981728538 1101 22-23 0.3662486154502034 1101 24-25 0.3922083495970412 1101 26-27 0.3805679359530387 1101 28-29 0.418535667650211 1101 30-31 0.48207067922379565 1101 32-33 0.4377866229439391 1101 34-35 0.4361025862900263 1101 36-37 0.47770177520299484 1101 38-39 0.5323092200790072 1101 40-41 0.5467448891731621 1101 42-43 0.5520233575144715 1101 44-45 0.45069139068110786 1101 46-47 0.5510884617626104 1101 48-49 0.5612426701286282 1101 50-51 0.5431300314261591 1101 52-53 0.49360794860449886 1101 54-55 0.49333831617142465 1101 56-57 0.4946953399566709 1101 58-59 0.6831624685845625 1101 60-61 0.6649290156152787 1101 62-63 0.691709484300226 1101 64-65 0.6804774950768504 1101 66-67 0.5743466854195702 1101 68-69 0.49368399726794365 1101 70-71 0.530958783694139 1101 72-73 0.5811262823073449 1101 74-75 0.5024605769395762 1101 76-77 0.0706162623107005 1101 78-79 0.4016156801981978 1101 80-81 0.6038587903400341 1101 82-83 0.7555330140976295 1101 84-85 0.6775990539606411 1101 86-87 0.5912946432184896 1101 88-89 0.8967086040885803 1101 90-91 0.827039564803222 1101 92-93 0.7764490939239792 1101 94-95 0.8678374189135454 1101 96-97 0.8437631253204927 1101 98-99 0.7032619944398988 1101 100-101 0.6516129009016041 2304 1 0.12625824740454306 2304 2 0.10206119789792467 2304 3 0.11265651690315792 2304 4 0.09786068952191584 2304 5 0.10347275377723975 2304 6 0.1610532716760531 2304 7 0.06807724658096248 2304 8 0.06964031230187118 2304 9 0.0952461861785423 2304 10-11 0.09768865942699989 2304 12-13 0.15914592460764965 2304 14-15 0.2637062033517523 2304 16-17 0.2816934478505573 2304 18-19 0.2503966177096544 2304 20-21 0.31367188618303743 2304 22-23 0.2463049989137076 2304 24-25 0.27531473543101725 2304 26-27 0.222829989524584 2304 28-29 0.2567449546867877 2304 30-31 0.31028617610976283 2304 32-33 0.20677042439940152 2304 34-35 0.2785294500069284 2304 36-37 0.26128623567450404 2304 38-39 0.317923777119681 2304 40-41 0.34645816183459743 2304 42-43 0.44826343231245147 2304 44-45 0.28657429421703995 2304 46-47 0.3864031006081845 2304 48-49 0.33424966123941147 2304 50-51 0.34705276331774115 2304 52-53 0.336377645057631 2304 54-55 0.37576689491557147 2304 56-57 0.32435716784583235 2304 58-59 0.37110160428855465 2304 60-61 0.26837978686371855 2304 62-63 0.18611107753117295 2304 64-65 0.19707167895833777 2304 66-67 0.3403364430972715 2304 68-69 0.43362768979758215 2304 70-71 0.3072605096626688 2304 72-73 0.15165461864197738 2304 74-75 0.2035133670496876 2304 76-77 0.07110438876951974 2304 78-79 0.23779206410039677 2304 80-81 0.3156319255695834 2304 82-83 0.4676112445556626 2304 84-85 0.45490332820402557 2304 86-87 0.41177831198817216 2304 88-89 0.6443004631841589 2304 90-91 0.4617390218235258 2304 92-93 0.441630180852286 2304 94-95 0.4221845250555063 2304 96-97 0.3786204926535035 2304 98-99 0.4539291581708582 2304 100-101 0.5474706783385663 2202 1 0.0850798172181797 2202 2 0.10159626916445319 2202 3 0.07583348086710373 2202 4 0.10806214519107016 2202 5 0.07589244083914082 2202 6 0.048861387827074054 2202 7 0.09910888602156831 2202 8 0.06610418001370277 2202 9 0.05031968834975231 2202 10-11 0.149503807812998 2202 12-13 0.09585505824614415 2202 14-15 0.07540618991098569 2202 16-17 0.10865372923488792 2202 18-19 0.09900594917821337 2202 20-21 0.19017555723721813 2202 22-23 0.1986301053738444 2202 24-25 0.11276506530733599 2202 26-27 0.12892126792196024 2202 28-29 0.17037556236059004 2202 30-31 0.1676314528190943 2202 32-33 0.15887295522713174 2202 34-35 0.22083916722314 2202 36-37 0.19889896230155557 2202 38-39 0.19319278035978016 2202 40-41 0.21971769514804151 2202 42-43 0.3072642413691469 2202 44-45 0.21040501269212797 2202 46-47 0.3204143106356483 2202 48-49 0.2526026142172171 2202 50-51 0.2331212137696852 2202 52-53 0.1910730574048003 2202 54-55 0.15558307046417497 2202 56-57 0.1618898906566173 2202 58-59 0.27369155874262674 2202 60-61 0.26989838001801303 2202 62-63 0.2334347627824087 2202 64-65 0.23540065056872805 2202 66-67 0.18428098169565033 2202 68-69 0.1470179537611287 2202 70-71 0.1873811237419929 2202 72-73 0.21824258680531727 2202 74-75 0.20788157699969645 2202 76-77 -0.17723206900831556 2202 78-79 0.2900904551558483 2202 80-81 0.3194462081771121 2202 82-83 0.4116240787088543 2202 84-85 0.43190316057020794 2202 86-87 0.48868212869945893 2202 88-89 0.6048977727997098 2202 90-91 0.4224817353887502 2202 92-93 0.5686059203061333 2202 94-95 0.4034507134080556 2202 96-97 0.46674972369682166 2202 98-99 0.36715871833498426 2202 100-101 0.29734348943755506 1102 1 0.10084355476608664 1102 2 0.11238751881669629 1102 3 0.06796849381644421 1102 4 0.07666177525162254 1102 5 0.09681775616822108 1102 6 0.03531050510983391 1102 7 0.0943367911090931 1102 8 0.07072831373153576 1102 9 0.13318160351015962 1102 10-11 0.1444008967548882 1102 12-13 0.05752990021242965 1102 14-15 0.1593184180760403 1102 16-17 0.20891366401966138 1102 18-19 0.13006190279321572 1102 20-21 0.09574477576656903 1102 22-23 0.1442900987159561 1102 24-25 0.038946576283549916 1102 26-27 0.06362640838847966 1102 28-29 0.09552484528843053 1102 30-31 0.11563512523198938 1102 32-33 0.17527014024535248 1102 34-35 0.10035456331832648 1102 36-37 0.08904780222088249 1102 38-39 0.20225604160973631 1102 40-41 0.10781881484748368 1102 42-43 0.19878757442658213 1102 44-45 0.1582899296562985 1102 46-47 0.18313035020523927 1102 48-49 0.06841645001092189 1102 50-51 0.039509031801479466 1102 52-53 -0.08212390709362438 1102 54-55 -0.017878277134883547 1102 56-57 0.015527719718051003 1102 58-59 0.09551984572900096 1102 60-61 0.11760897404683845 1102 62-63 0.10395704975639575 1102 64-65 0.06829014893360608 1102 66-67 0.07033678764115336 1102 68-69 -0.014223359717156825 1102 70-71 -0.18624411877325997 1102 72-73 -0.06320476391267249 1102 74-75 -0.006610051598016753 1102 76-77 -0.0824435991888599 1102 78-79 0.06693146541942241 1102 80-81 0.060432625864486056 1102 82-83 0.12505897705936775 1102 84-85 0.04706491701324467 1102 86-87 0.09946787264929213 1102 88-89 0.397551196854387 1102 90-91 0.0420840049049076 1102 92-93 0.1064772771992537 1102 94-95 0.20171036452153857 1102 96-97 0.07778548487476655 1102 98-99 0.10720048875010946 1102 100-101 0.16598366480328863 2305 1 0.1311627923600014 2305 2 0.0953544487786715 2305 3 0.07941746312145881 2305 4 0.09504147116248163 2305 5 0.06715100532485963 2305 6 0.059656148697669664 2305 7 0.0354157887558344 2305 8 0.0439213570476511 2305 9 0.09340861899699604 2305 10-11 0.06328873818611669 2305 12-13 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-0.8822584143509005 1213 96-97 -0.8650280180851411 1213 98-99 -0.9560140758149416 1213 100-101 -0.765254269665494 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 16.0 8 54.0 9 245.0 10 364.0 11 226.0 12 175.0 13 205.0 14 444.0 15 939.0 16 1779.0 17 2877.0 18 4030.0 19 4919.0 20 6374.0 21 7990.0 22 10059.0 23 12762.0 24 16196.0 25 20181.0 26 25427.0 27 32420.0 28 42244.0 29 55242.0 30 65278.0 31 81227.0 32 108817.0 33 154322.0 34 235435.0 35 411598.0 36 835364.0 37 1422263.0 38 395863.0 39 902.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.93797584053028 32.24777761742358 15.843264743935444 18.97098179811069 2 30.77315641100369 24.667354357183356 29.942821929019924 14.616667302793033 3 32.63365961238301 22.979001447086553 24.24217530600509 20.145163634525336 4 28.421759726389173 22.845280589897506 28.660294448170713 20.07266523554261 5 31.471597884555454 21.02914966924378 27.888521339848953 19.61073110635182 6 28.470412667390754 21.493151193924934 30.193969673707617 19.842466464976695 7 31.21731584836803 22.309760512325223 24.578507303783873 21.89441633552287 8 27.62637829836787 25.203798457979136 25.35237398568387 21.817449257969127 9 26.721553840176917 22.683373114401387 28.326184705314674 22.268888340107026 10-11 22.007762216188677 29.273759227726597 31.051758934769857 17.666719621314865 12-13 22.08319420702046 28.641572787474562 30.131144822719165 19.144088182785815 14-15 22.102657146171982 28.449824922015544 30.3985959385143 19.048921993298176 16-17 22.382707981063064 27.832758515877735 30.62826838037214 19.156265122687056 18-19 21.588987211837914 27.846410616957478 31.023533221088623 19.54106895011598 20-21 21.97238183657855 28.113659520397793 30.558697570443833 19.355261072579825 22-23 21.575383756755507 28.9184718633988 29.980622903142066 19.525521476703627 24-25 21.340809461111657 28.185609203897542 30.53138626427082 19.94219507071998 26-27 21.31550764021468 28.267669505138343 30.490248688336923 19.926574166310058 28-29 21.313776196926522 29.139798753209174 30.084547513205102 19.461877536659202 30-31 20.731429891586373 29.470163187897995 30.254456444343454 19.54395047617218 32-33 20.560017006059038 29.808780414317948 29.99353931526347 19.637663264359542 34-35 20.982122658475717 29.470820378051165 30.296440784513162 19.250616178959955 36-37 20.6251208547607 28.981976368707812 31.130328510096188 19.2625742664353 38-39 20.96111901291163 29.05400913654286 30.46320194550277 19.521669905042742 40-41 20.803125251988543 29.11177687344873 30.349746829715524 19.7353510448472 42-43 21.102057581962033 29.12401102095498 30.011349561436766 19.762581835646216 44-45 20.64726897067546 28.85226865281926 30.381327597513923 20.11913477899136 46-47 21.03376182298273 29.068005943277992 30.009294587097386 19.888937646641892 48-49 20.688406937381735 29.76750981848645 29.90347024549597 19.640612998635845 50-51 21.17333316481454 29.33830885071236 29.375075438491166 20.113282545981935 52-53 20.737917586273706 29.101061922587572 29.32960346658204 20.831417024556682 54-55 21.26283245571681 29.18918850596215 29.519489153873185 20.028489884447858 56-57 20.98460885814512 28.85339475683098 29.690143280623044 20.471853104400857 58-59 21.024444255949383 29.384582303440276 29.57259559343773 20.018377847172612 60-61 21.02878366112678 29.54782364033689 29.31546676707699 20.107925931459334 62-63 20.619196306362987 29.508071995185293 29.1938317717795 20.678899926672226 64-65 20.870579065992743 29.25546202278283 29.314478804961354 20.559480106263074 66-67 20.99428466097674 29.50133676503998 29.10061699668271 20.403761577300568 68-69 21.082181174648824 29.673759223853242 29.129950858786163 20.11410874271177 70-71 20.99188000009103 30.182236637324287 28.952225938940572 19.87365742364411 72-73 20.668627877341315 30.138165479995145 28.911098244265542 20.282108398397995 74-75 20.84240292263064 29.54680666598063 28.931924541354164 20.678865870034567 76-77 20.935085164057128 29.186364647813246 28.985111461746975 20.89343872638265 78-79 20.611842416284315 28.824065663505127 29.734593904631986 20.829498015578572 80-81 20.566473789707405 29.240248986466927 29.37846665977369 20.814810564051978 82-83 20.124682729127926 29.584261570094444 29.234140374295897 21.05691532648173 84-85 20.146227676074542 29.51153585826444 29.3432015000692 20.999034965591814 86-87 20.286073217632868 29.34714841990137 29.829920518503123 20.536857843962636 88-89 20.418573442810366 29.87912002065019 29.48080490244882 20.221501634090615 90-91 20.730507448467648 29.587219084096557 28.966935885444244 20.715337581991548 92-93 20.41970588346856 29.65763530915615 29.02232994610013 20.900328861275163 94-95 19.992197451504605 29.875733163966284 28.950602738021974 21.181466646507133 96-97 20.07166981620103 30.237527312960893 28.830375703967704 20.86042716687037 98-99 20.31634421511447 29.99397441998419 28.776468428539857 20.913212936361482 100-101 20.429605983418412 30.033182645619178 28.29301195047998 21.24419942048243 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 198.0 1 126.5 2 84.0 3 145.5 4 139.0 5 90.5 6 89.5 7 87.0 8 79.0 9 80.5 10 75.5 11 81.5 12 87.5 13 89.0 14 99.5 15 122.0 16 151.0 17 182.0 18 222.5 19 315.5 20 500.0 21 754.0 22 1172.0 23 1859.5 24 2836.5 25 4489.0 26 7491.5 27 11868.0 28 16845.5 29 22358.0 30 28303.5 31 32788.0 32 40132.5 33 55995.0 34 78998.0 35 112192.5 36 156175.5 37 209406.5 38 262401.0 39 281517.0 40 287354.0 41 276605.0 42 243194.0 43 228312.5 44 215500.5 45 205257.5 46 209369.5 47 195175.0 48 160152.5 49 130882.5 50 105283.5 51 84876.0 52 70020.5 53 56272.5 54 45490.0 55 35220.5 56 24324.5 57 15607.5 58 10333.0 59 7405.0 60 5510.5 61 4025.5 62 2795.5 63 1895.5 64 1347.0 65 957.0 66 674.0 67 526.5 68 384.5 69 253.5 70 178.5 71 133.0 72 97.5 73 69.5 74 52.0 75 40.5 76 35.0 77 23.0 78 13.5 79 12.0 80 10.0 81 10.0 82 8.5 83 5.5 84 3.0 85 3.5 86 3.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16801319031190498 2 0.0 3 3.033185322315119E-4 4 0.02118174416750058 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.434457035814589E-4 12-13 0.0 14-15 0.0 16-17 0.0013522951228654907 18-19 0.0 20-21 0.0 22-23 5.055308870525199E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0010616148628102917 38-39 9.099555966945357E-4 40-41 0.005156415047935702 42-43 0.003462886576309761 44-45 0.0011500827680444826 46-47 0.004322289084299044 48-49 0.007747260844079867 50-51 0.005042670598348885 52-53 0.0016050605663917505 54-55 0.01129861532562382 56-57 0.018894216903587932 58-59 0.02354510106447111 60-61 0.011842061029205278 62-63 7.077432418735278E-4 64-65 0.014723587085404641 66-67 0.03421180278127928 68-69 0.020208597209924482 70-71 0.03624656460166568 72-73 0.03025602359009331 74-75 0.029573556892572413 76-77 0.03834451778293363 78-79 0.01708694398237517 80-81 0.013143803063365517 82-83 8.973173245182229E-4 84-85 0.010603510355926604 86-87 0.02151033924408472 88-89 0.02087842563526907 90-91 0.025971649322323206 92-93 0.04251514760111692 94-95 0.028284453130588486 96-97 0.029194408727283024 98-99 0.036170734968607794 100-101 0.017832602040777638 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3956237.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.55416518635533 #Duplication Level Percentage of deduplicated Percentage of total 1 86.69652674985632 36.02601793646802 2 6.132481930914057 5.09660334319084 3 2.0203192173360063 2.5185803545904553 4 1.0244777426800857 1.702852693962908 5 0.7258643288471516 1.5081343111898742 6 0.49149759532647974 1.2254263358935777 7 0.3163325810149791 0.9201455427725799 8 0.23422804589318594 0.7786520728258133 9 0.18495932386513822 0.691724726698369 >10 1.7380725589801278 14.678174159616294 >50 0.2255118900058886 6.544994267429387 >100 0.17369581702358705 14.195606059903715 >500 0.025597560737866377 7.4924915811605 >1k 0.010373322457735965 6.1450805664861905 >5k 0.0 0.0 >10k+ 6.133506145977292E-5 0.4755160478114271 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 18657 0.4715844879869431 TruSeq Adapter, Index 2 (97% over 37bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.066370644630238E-4 0.0 0.0 2 6.066370644630238E-4 0.0 0.0 3 8.846790523419098E-4 0.0 0.0 4 0.0011374444958681697 0.0 0.0 5 0.0013143803063365516 0.0 0.0 6 0.002350718624794217 0.0 0.0 7 0.003109014955372997 0.0 0.0 8 0.0042970125399464185 0.0 0.0 9 0.004853096515704191 0.0 0.0 10-11 0.00674883734215114 0.0 0.0 12-13 0.010110617741050397 0.0 0.0 14-15 0.014862608079344083 0.0 0.0 16-17 0.021624083693671536 0.0 0.0 18-19 0.030761554477145833 0.0 0.0 20-21 0.03979791908320963 0.0 0.0 22-23 0.051513597390651776 0.0 0.0 24-25 0.06580748322206177 0.0 0.0 26-27 0.0804173258578796 0.0 0.0 28-29 0.09664486733226549 0.0 0.0 30-31 0.11710622998571624 0.0 0.0 32-33 0.1350904912926096 0.0 0.0 34-35 0.15713163796809948 0.0 0.0 36-37 0.18100533410915476 0.0 0.0 38-39 0.2076215353124699 0.0 0.0 40-41 0.2377638144529764 0.0 0.0 42-43 0.27025681221827713 0.0 0.0 44-45 0.30800733120892404 0.0 0.0 46-47 0.3488163120662387 0.0 0.0 48-49 0.3926584782458685 0.0 0.0 50-51 0.44069655078803416 0.0 0.0 52-53 0.4912875543098151 0.0 0.0 54-55 0.5459480814723688 0.0 0.0 56-57 0.6041346865721138 0.0 0.0 58-59 0.6656704337985818 0.0 0.0 60-61 0.730846003411828 0.0 2.5276544352625994E-5 62-63 0.798764078087334 0.0 2.5276544352625994E-5 64-65 0.8704230813270286 0.0 2.5276544352625994E-5 66-67 0.9466192242780198 0.0 2.5276544352625994E-5 68-69 1.0301455650912723 0.0 2.5276544352625994E-5 70-71 1.119649808643921 0.0 2.5276544352625994E-5 72-73 1.2147654450428527 0.0 2.5276544352625994E-5 74-75 1.3168700459552851 0.0 5.055308870525199E-5 76-77 1.4246492310748824 0.0 5.055308870525199E-5 78-79 1.5395943165184494 0.0 5.055308870525199E-5 80-81 1.6657621876545818 0.0 5.055308870525199E-5 82-83 1.800915870308073 0.0 5.055308870525199E-5 84-85 1.946281276880025 0.0 5.055308870525199E-5 86-87 2.1041206580899985 0.0 5.055308870525199E-5 88-89 2.2729174212768344 0.0 5.055308870525199E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGAGT 20 0.002061101 71.36608 1 AACGCGC 35 2.7664457E-4 54.374157 1 CGCCGAG 50 2.8011767E-5 47.57739 1 CGGACGG 40 5.346702E-4 47.57739 1 CGAGGCG 155 0.0 42.973125 1 TGCGGCC 115 5.4569682E-11 41.304176 2 CGAGCGG 85 3.597488E-7 39.181377 1 CGACGGG 135 7.2759576E-12 38.76676 1 GGTACGC 50 0.0016034187 38.061913 1 CGACGCC 50 0.0016034187 38.061913 1 CGGGACG 50 0.0016034187 38.061913 1 GGTACGG 65 1.316106E-4 36.597992 1 AGGCGCG 105 4.5962224E-8 36.24944 1 TTGTGTT 25470 0.0 36.182728 1 CGGGCTG 395 0.0 36.134724 1 ATGTCGC 80 1.0663098E-5 35.68304 1 CGGCGCC 55 0.0025598656 34.601738 1 GTCGCCG 70 2.0346849E-4 33.98385 1 GTCGACC 70 2.0346849E-4 33.98385 1 CGGTGCG 70 2.0346849E-4 33.98385 1 >>END_MODULE