FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_44065-1.341000000357de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_44065-1.341000000357de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3956237
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT186570.4715844879869431TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT200.00206110171.366081
AACGCGC352.7664457E-454.3741571
CGCCGAG502.8011767E-547.577391
CGGACGG405.346702E-447.577391
CGAGGCG1550.042.9731251
TGCGGCC1155.4569682E-1141.3041762
CGAGCGG853.597488E-739.1813771
CGACGGG1357.2759576E-1238.766761
GGTACGC500.001603418738.0619131
CGACGCC500.001603418738.0619131
CGGGACG500.001603418738.0619131
GGTACGG651.316106E-436.5979921
AGGCGCG1054.5962224E-836.249441
TTGTGTT254700.036.1827281
CGGGCTG3950.036.1347241
ATGTCGC801.0663098E-535.683041
CGGCGCC550.002559865634.6017381
GTCGCCG702.0346849E-433.983851
GTCGACC702.0346849E-433.983851
CGGTGCG702.0346849E-433.983851
Produced by FastQC (version 0.11.2)