##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_41442-1.34100000035721.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3140202 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67573200704923 33.0 33.0 33.0 33.0 33.0 2 32.71033965330893 33.0 33.0 33.0 33.0 33.0 3 32.74868654946402 33.0 33.0 33.0 33.0 33.0 4 36.66515720963174 37.0 37.0 37.0 37.0 37.0 5 36.674217454800676 37.0 37.0 37.0 37.0 37.0 6 36.57874493424308 37.0 37.0 37.0 37.0 37.0 7 36.460681510297746 37.0 37.0 37.0 37.0 37.0 8 36.42986342916793 37.0 37.0 37.0 37.0 37.0 9 36.46259348920866 37.0 37.0 37.0 37.0 37.0 10-11 36.404493564426744 37.0 37.0 37.0 37.0 37.0 12-13 36.3840518858341 37.0 37.0 37.0 37.0 37.0 14-15 38.267681665064856 40.0 37.0 40.0 37.0 40.0 16-17 38.29803273802131 40.0 37.0 40.0 37.0 40.0 18-19 38.27234378552717 40.0 37.0 40.0 37.0 40.0 20-21 38.26825025905977 40.0 37.0 40.0 37.0 40.0 22-23 38.04901022290923 40.0 37.0 40.0 35.0 40.0 24-25 38.12113313092597 40.0 37.0 40.0 37.0 40.0 26-27 38.05537080098669 40.0 37.0 40.0 37.0 40.0 28-29 37.85824319581988 40.0 37.0 40.0 33.0 40.0 30-31 37.81791059938182 40.0 37.0 40.0 33.0 40.0 32-33 37.69395073947472 40.0 37.0 40.0 33.0 40.0 34-35 37.693631333270915 40.0 37.0 40.0 33.0 40.0 36-37 37.61156065756279 40.0 37.0 40.0 33.0 40.0 38-39 37.33487686460934 40.0 37.0 40.0 33.0 40.0 40-41 37.194715339968575 40.0 37.0 40.0 33.0 40.0 42-43 36.95740289955869 38.5 37.0 40.0 33.0 40.0 44-45 36.794271355791764 37.0 37.0 40.0 33.0 40.0 46-47 36.833265503302016 37.0 37.0 40.0 33.0 40.0 48-49 36.520507438693436 37.0 37.0 40.0 33.0 40.0 50-51 36.46309297936884 37.0 37.0 40.0 33.0 40.0 52-53 36.43985705378189 37.0 37.0 40.0 33.0 40.0 54-55 36.05700429462818 37.0 37.0 40.0 30.0 40.0 56-57 35.890737602230686 37.0 37.0 40.0 27.0 40.0 58-59 35.70062626544407 37.0 37.0 40.0 27.0 40.0 60-61 35.33744262311788 37.0 33.0 40.0 27.0 40.0 62-63 35.10596897906568 37.0 33.0 38.5 27.0 40.0 64-65 34.843618977377886 37.0 33.0 37.0 27.0 40.0 66-67 34.16397098021083 37.0 33.0 37.0 27.0 40.0 68-69 34.088742061816404 37.0 33.0 37.0 27.0 40.0 70-71 33.75271081287127 37.0 33.0 37.0 27.0 38.5 72-73 32.94570412986171 37.0 33.0 37.0 22.0 37.0 74-75 31.976050744506246 37.0 33.0 37.0 18.5 37.0 76-77 29.398661137086087 33.0 27.0 35.0 15.0 37.0 78-79 31.528866455087922 33.0 33.0 37.0 15.0 37.0 80-81 31.786939821068835 37.0 33.0 37.0 15.0 37.0 82-83 31.648217853501144 37.0 33.0 37.0 15.0 37.0 84-85 31.500015922542563 37.0 33.0 37.0 15.0 37.0 86-87 31.40221234175381 37.0 33.0 37.0 15.0 37.0 88-89 31.156702339531023 33.0 33.0 37.0 15.0 37.0 90-91 31.125054057032003 33.0 33.0 37.0 15.0 37.0 92-93 30.975888334572108 33.0 33.0 37.0 15.0 37.0 94-95 30.8074990080256 33.0 33.0 37.0 15.0 37.0 96-97 30.70170692840779 33.0 33.0 37.0 15.0 37.0 98-99 30.591301292082484 33.0 33.0 37.0 15.0 37.0 100-101 29.083056917994448 33.0 27.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.17581110805274847 2306 2 0.17251989497923148 2306 3 0.1319066580135697 2306 4 0.20442467537202447 2306 5 0.20307313232629554 2306 6 0.2116307410497953 2306 7 0.23283915220753215 2306 8 0.201046781047431 2306 9 0.22142282472306363 2306 10-11 0.2265589712749687 2306 12-13 0.27678410363224515 2306 14-15 0.4599535063665954 2306 16-17 0.34539670769848385 2306 18-19 0.4665420594296208 2306 20-21 0.4121987065209396 2306 22-23 0.4555372879898556 2306 24-25 0.423508624897174 2306 26-27 0.42710979287030426 2306 28-29 0.4346641234212143 2306 30-31 0.4768177158024116 2306 32-33 0.3805112555774883 2306 34-35 0.4376909982072732 2306 36-37 0.44388443897899066 2306 38-39 0.5565646452955164 2306 40-41 0.5179961918794334 2306 42-43 0.4654801982515835 2306 44-45 0.40908308851052766 2306 46-47 0.32305915335049207 2306 48-49 -0.08941263843967562 2306 50-51 -0.020196307886124032 2306 52-53 0.055434430616763564 2306 54-55 -0.0918308873260898 2306 56-57 -0.11417229813596208 2306 58-59 0.025775653711228586 2306 60-61 -0.20813723047063348 2306 62-63 -0.08095003576418947 2306 64-65 -0.18329286114591525 2306 66-67 -0.1075212111445012 2306 68-69 0.2426400998330962 2306 70-71 0.49160098069631886 2306 72-73 0.46746695038989117 2306 74-75 0.4522491369476711 2306 76-77 -0.118721096243533 2306 78-79 0.3588630447156511 2306 80-81 0.4797774518964566 2306 82-83 0.492603772537187 2306 84-85 0.5975575308193406 2306 86-87 0.5644757872981216 2306 88-89 0.6897797564530066 2306 90-91 0.5495690539985247 2306 92-93 0.472548086138314 2306 94-95 0.5557123553550447 2306 96-97 0.3858539505314731 2306 98-99 0.2611770954039301 2306 100-101 0.22837949527682255 2307 1 0.13807840245918612 2307 2 0.08837272132640095 2307 3 0.12796370754076491 2307 4 0.18383615335718417 2307 5 0.1771740724976425 2307 6 0.22872498351907922 2307 7 0.2092913198114985 2307 8 0.27307904060695876 2307 9 0.22763310156461358 2307 10-11 0.2317204848181973 2307 12-13 0.2246891340204158 2307 14-15 0.4039032008868162 2307 16-17 0.4380672306178539 2307 18-19 0.3920742718447414 2307 20-21 0.43138628053366546 2307 22-23 0.4186188222446603 2307 24-25 0.44107391191073475 2307 26-27 0.4489144807823138 2307 28-29 0.48279736470308876 2307 30-31 0.4332760601227861 2307 32-33 0.437302087828094 2307 34-35 0.5297048954196768 2307 36-37 0.47428539017733584 2307 38-39 0.4857978934492948 2307 40-41 0.5406157189331253 2307 42-43 0.4397477419640339 2307 44-45 0.4493785720763057 2307 46-47 0.520653150509041 2307 48-49 0.2370887511217603 2307 50-51 0.4000013949826524 2307 52-53 0.38877115994554146 2307 54-55 0.2013066448959151 2307 56-57 0.35088550570175414 2307 58-59 0.46849639741025584 2307 60-61 0.3382876253393974 2307 62-63 0.2853384368688836 2307 64-65 0.24704448505544008 2307 66-67 0.24846920495683378 2307 68-69 0.40060123206596643 2307 70-71 0.4171907252695135 2307 72-73 0.19518182900502978 2307 74-75 0.1540279993131719 2307 76-77 -0.13303741444111594 2307 78-79 0.1659499278630463 2307 80-81 0.3963767979475854 2307 82-83 0.5533427491359788 2307 84-85 0.5415561676268048 2307 86-87 0.4338492221437491 2307 88-89 0.7114108490685958 2307 90-91 0.5521733829737947 2307 92-93 0.5143721671819499 2307 94-95 0.48209696262615154 2307 96-97 0.644123399197607 2307 98-99 0.546261324081474 2307 100-101 0.3979573290951812 2201 1 0.13495688335451206 2201 2 0.11820234623271375 2201 3 0.08130312969416309 2201 4 0.1185379529950481 2201 5 0.10882986398831918 2201 6 0.16102721273038867 2201 7 0.17062931005340687 2201 8 0.15137175783480217 2201 9 0.16989079129687923 2201 10-11 0.2592887762888907 2201 12-13 0.1939150228523019 2201 14-15 0.3106963104520233 2201 16-17 0.36071704196032073 2201 18-19 0.3158921058548785 2201 20-21 0.38155803799725874 2201 22-23 0.3041909555850282 2201 24-25 0.36408429806338205 2201 26-27 0.35143662666788344 2201 28-29 0.37988232212130413 2201 30-31 0.3298817826547804 2201 32-33 0.39165331685882165 2201 34-35 0.4330484726733772 2201 36-37 0.4905418205945935 2201 38-39 0.42819881428344786 2201 40-41 0.39682627544489435 2201 42-43 0.42021557429615086 2201 44-45 0.37728178860337636 2201 46-47 0.3569495897478916 2201 48-49 0.22999311829199343 2201 50-51 0.1961453819931691 2201 52-53 0.4273007258813877 2201 54-55 0.21318304025050594 2201 56-57 0.26303290149263603 2201 58-59 0.3052556908514319 2201 60-61 0.2570438280251892 2201 62-63 0.26143622236023134 2201 64-65 0.29535152139818877 2201 66-67 0.17195882599570922 2201 68-69 0.17857324746540826 2201 70-71 0.23811908654590042 2201 72-73 0.06310297638803064 2201 74-75 0.19853511652056 2201 76-77 0.007091345724898446 2201 78-79 0.25231708371286743 2201 80-81 0.34212656981660317 2201 82-83 0.49469290902743523 2201 84-85 0.529312405471142 2201 86-87 0.42496789500471266 2201 88-89 0.6531038047352737 2201 90-91 0.28285596207651764 2201 92-93 0.41173100164435184 2201 94-95 0.42572164040611327 2201 96-97 0.4336719635305464 2201 98-99 0.3767759811978024 2201 100-101 0.356048947458806 1101 1 0.1394625314031117 1101 2 0.09494331280613721 1101 3 0.0894106834205246 1101 4 0.11002678617103356 1101 5 0.15916178214104093 1101 6 0.1038461557944288 1101 7 0.11571433592948921 1101 8 0.047743607556583356 1101 9 0.09766666474877184 1101 10-11 0.11915586864063954 1101 12-13 0.15702078859214197 1101 14-15 0.22793105767907917 1101 16-17 0.22776484028011623 1101 18-19 0.2306393680126959 1101 20-21 0.21355964309051245 1101 22-23 0.1668949410596312 1101 24-25 0.23362679579281576 1101 26-27 0.1857740206999523 1101 28-29 0.2197089517725601 1101 30-31 0.3667606133232937 1101 32-33 0.3561897642914289 1101 34-35 0.28714438518409224 1101 36-37 0.26664418155572633 1101 38-39 0.432902174255247 1101 40-41 0.3433473450235027 1101 42-43 0.2889834078531237 1101 44-45 0.22271873780311324 1101 46-47 0.3491858870832232 1101 48-49 0.24765323862718702 1101 50-51 0.29502951851656434 1101 52-53 0.22631943910044328 1101 54-55 0.26620853962909763 1101 56-57 0.41240406448719824 1101 58-59 0.411783616003234 1101 60-61 0.18089812252704718 1101 62-63 0.22164465050364157 1101 64-65 0.3172237441208594 1101 66-67 0.20280695555122463 1101 68-69 0.2789658635685157 1101 70-71 0.24386774594596972 1101 72-73 0.1146543130493285 1101 74-75 -0.04204753700742714 1101 76-77 -0.3037880355732696 1101 78-79 0.03290773930922697 1101 80-81 0.2662602737720512 1101 82-83 0.41236987582236395 1101 84-85 0.38766078167910933 1101 86-87 0.3382602001892536 1101 88-89 0.4551710642257376 1101 90-91 0.18669114442579726 1101 92-93 0.1517812563495653 1101 94-95 0.35911884989925014 1101 96-97 0.3070673089941067 1101 98-99 0.2645669579687926 1101 100-101 0.3577392479146404 2304 1 0.12779534798443848 2304 2 0.09257091478911406 2304 3 0.11895555750582076 2304 4 0.17189928006443722 2304 5 0.14619926297581287 2304 6 0.10090091045134386 2304 7 0.1305207676998208 2304 8 0.1282140302950836 2304 9 0.16480906472511236 2304 10-11 0.14475164834387755 2304 12-13 0.19075826539255303 2304 14-15 0.3015706296637575 2304 16-17 0.3252241080867293 2304 18-19 0.2727531296410248 2304 20-21 0.30517543419603754 2304 22-23 0.3987420377571951 2304 24-25 0.3018931112931398 2304 26-27 0.24129657270585625 2304 28-29 0.34225174775934164 2304 30-31 0.33695350567669635 2304 32-33 0.27312553579414356 2304 34-35 0.40000205914428477 2304 36-37 0.37243778324970833 2304 38-39 0.4879664001808237 2304 40-41 0.41309110514255565 2304 42-43 0.5511992886790296 2304 44-45 0.472265675681804 2304 46-47 0.4623101343571747 2304 48-49 0.39456398583130436 2304 50-51 0.3657993086316722 2304 52-53 0.3263572431663988 2304 54-55 0.3727038953706696 2304 56-57 0.4026071536515943 2304 58-59 0.3999754709950665 2304 60-61 0.26628170762227654 2304 62-63 0.2148926385863703 2304 64-65 0.18403617825105556 2304 66-67 0.11242469890244422 2304 68-69 0.1958833116719063 2304 70-71 0.23197240405946928 2304 72-73 0.09586929265329758 2304 74-75 0.0374661310429758 2304 76-77 0.07816675931475459 2304 78-79 0.2390424455473621 2304 80-81 0.35058785243287005 2304 82-83 0.45690722847370324 2304 84-85 0.3522275064180924 2304 86-87 0.27228077350912727 2304 88-89 0.6489572459073862 2304 90-91 0.31085556125227143 2304 92-93 0.3000787955652555 2304 94-95 0.25782798249042216 2304 96-97 0.2800709947298081 2304 98-99 0.3210686063514423 2304 100-101 0.45077405771212753 2202 1 0.07278434270847356 2202 2 0.06303139947002734 2202 3 0.013881661970557957 2202 4 0.015147749984116388 2202 5 0.07177434422204954 2202 6 0.07325121937611101 2202 7 0.10267453157899809 2202 8 0.04368885694692892 2202 9 0.10413024883214206 2202 10-11 0.12122732558283644 2202 12-13 0.09529159972315426 2202 14-15 0.10028606443345467 2202 16-17 0.05634404502610124 2202 18-19 0.03696327410786182 2202 20-21 0.15337513559716598 2202 22-23 0.21553005956247517 2202 24-25 0.17533513860750105 2202 26-27 0.03941530835194129 2202 28-29 0.1237036297508709 2202 30-31 0.15754049805133263 2202 32-33 0.17204320835175224 2202 34-35 0.22385531437485184 2202 36-37 0.19258140656801004 2202 38-39 0.13121428836041105 2202 40-41 0.2073583058466255 2202 42-43 0.20356740716120214 2202 44-45 0.22467854659824837 2202 46-47 0.3226183402582663 2202 48-49 0.1432429164101805 2202 50-51 0.23899347311369468 2202 52-53 0.16054781207912328 2202 54-55 0.07364087089560911 2202 56-57 0.1997932352705618 2202 58-59 0.29747681419139127 2202 60-61 0.20093505077870333 2202 62-63 0.27046772398788477 2202 64-65 0.23869481524233294 2202 66-67 0.19823219362626787 2202 68-69 0.15525728453705767 2202 70-71 -0.09472680681009393 2202 72-73 -0.07730746117881182 2202 74-75 0.034969011695253016 2202 76-77 -0.3198901456700476 2202 78-79 0.037345468144224014 2202 80-81 0.24536519764534503 2202 82-83 0.2865550305557534 2202 84-85 0.296211752896383 2202 86-87 0.31474219063630215 2202 88-89 0.486360266717476 2202 90-91 0.29395164850996025 2202 92-93 0.3854034460121767 2202 94-95 0.28456517267746406 2202 96-97 0.15502616957802573 2202 98-99 0.24402772705960274 2202 100-101 0.23934311420864773 1102 1 0.08549949321534456 1102 2 0.05810992190055231 1102 3 0.0815796756802385 1102 4 0.049306036360583505 1102 5 0.02689986443643022 1102 6 0.007777648544930571 1102 7 0.03809484208244385 1102 8 0.08998685314053745 1102 9 0.08160634471318673 1102 10-11 0.10543519806586232 1102 12-13 0.06758121727556698 1102 14-15 0.08033710776934555 1102 16-17 0.17594867781834722 1102 18-19 0.1476083894152609 1102 20-21 0.16163065129572374 1102 22-23 0.11811397270330559 1102 24-25 0.18666555479489233 1102 26-27 0.2606367083938892 1102 28-29 0.2595158775375168 1102 30-31 0.2618014097736463 1102 32-33 0.2525345474868885 1102 34-35 0.2458391397398998 1102 36-37 0.13617924107391133 1102 38-39 0.26263817487779306 1102 40-41 0.3523330816098138 1102 42-43 0.26409745265289075 1102 44-45 0.1568103192181951 1102 46-47 0.35383010402001247 1102 48-49 0.3603868226325275 1102 50-51 0.3386250860247273 1102 52-53 0.2056552286924429 1102 54-55 0.2250857205060015 1102 56-57 0.28542591893367586 1102 58-59 0.38867762857859844 1102 60-61 0.4172694801488248 1102 62-63 0.3906016864827393 1102 64-65 0.40573183558576176 1102 66-67 0.4366009566877409 1102 68-69 0.328772501590322 1102 70-71 0.1613019757291525 1102 72-73 0.1652908703485565 1102 74-75 0.3420789350361986 1102 76-77 0.42884528341070194 1102 78-79 0.6209165326264383 1102 80-81 0.6960022560501891 1102 82-83 0.8352082904681808 1102 84-85 0.8098829582278135 1102 86-87 0.7226965985351939 1102 88-89 0.868128336636147 1102 90-91 0.7520257067861884 1102 92-93 0.7735588342978339 1102 94-95 0.8603281507216707 1102 96-97 0.7461535728307602 1102 98-99 0.7381517825936008 1102 100-101 0.8282871774892904 2305 1 0.0654191062379823 2305 2 0.04960042144438148 2305 3 0.029772014670655267 2305 4 0.06326342865875745 2305 5 0.0352263182192587 2305 6 0.021405668105664688 2305 7 0.012410111212794561 2305 8 0.03882380573501365 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-0.8625653829400655 1213 96-97 -0.8390095361641947 1213 98-99 -0.9810594929439667 1213 100-101 -0.8682374033571314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 23.0 10 28.0 11 27.0 12 49.0 13 114.0 14 401.0 15 1248.0 16 2657.0 17 4415.0 18 5685.0 19 6591.0 20 8397.0 21 10929.0 22 14613.0 23 19770.0 24 25291.0 25 32154.0 26 42461.0 27 61361.0 28 94424.0 29 113858.0 30 75758.0 31 62736.0 32 81546.0 33 114440.0 34 174542.0 35 305971.0 36 627711.0 37 1013618.0 38 239140.0 39 242.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.66541244739265 23.7414904794805 21.972696807482446 13.620400265644408 2 27.86983130384606 28.718534667515023 30.690095732694907 12.721538295944018 3 34.44180023329776 17.767028969238517 32.42345295336943 15.367717844094297 4 27.371730596987355 17.488638549811377 30.98414302113287 24.155487832068403 5 36.405651610947324 16.25694780144717 31.669172874866014 15.6682277127395 6 31.9113865923275 17.093104201576843 35.24846490767155 15.747044298424115 7 30.254136517332324 26.08016936490073 26.541381732767512 17.124312384999435 8 27.092110634920942 33.55210906814275 22.887858806535373 16.467921490400936 9 34.82686782570038 23.467120904960893 23.917697014395888 17.788314254942836 10-11 26.373339766080473 29.90256139571506 27.180888371933797 16.543210466270672 12-13 21.93655057859335 28.579658251284474 27.124783692259285 22.35900747786289 14-15 21.0786599078658 29.150831698088215 27.954889526215194 21.815618867830796 16-17 24.609953255693124 25.675962930014745 28.582112711467555 21.13197110282458 18-19 19.548487645062323 25.71616411937831 33.03505634350911 21.70029189205026 20-21 24.13511933308749 26.01192534747765 32.46784760980345 17.38510770963142 22-23 19.662602895942186 34.42723315522292 28.3423407586873 17.567823190147593 24-25 20.011642563121733 25.34220728475429 32.67264335224295 21.973506799881026 26-27 19.851509552570185 25.52856472290636 28.1467561640939 26.473169560429554 28-29 23.677696530350595 30.120132399125914 27.928521795731616 18.273649274791875 30-31 18.892335588602265 26.539980549022008 31.97974206754852 22.587941794827213 32-33 18.583167579665258 30.87828426324166 28.417168704433664 22.121379452659415 34-35 18.996389404248518 26.532210348251482 32.79564499353863 21.67575525396137 36-37 23.358069738766236 27.141758933257243 28.331771849730714 21.168399478245806 38-39 23.935200540223722 31.263393935476003 27.360258431004205 17.44114709329607 40-41 23.741317412562672 30.85248032753629 27.272918352548004 18.133283907353036 42-43 19.14435168196157 30.91912562615472 32.076111625160806 17.860411066722907 44-45 18.749150125359495 26.665539338806287 32.52181650095902 22.06349403487519 46-47 23.47458620296516 26.321914889687033 28.008376257059563 22.195122650288244 48-49 19.463230990008135 31.28445512259097 31.381415141750484 17.870898745650415 50-51 23.759911899183418 26.746514931820908 31.001016229416255 18.492556939579412 52-53 19.054951861491023 26.72502891972775 26.93543237878992 27.284586839991302 54-55 23.76922242734496 26.669054250762258 31.018236673068767 18.543486648824015 56-57 19.382801653311095 26.784512486945154 30.84851474962567 22.984171110118073 58-59 19.295964279821654 26.657772508738613 31.53918090507002 22.50708230636971 60-61 23.124282012108218 26.88949252687468 31.57243767299786 18.41378778801924 62-63 18.96378634044359 26.969228699287385 31.200145214489922 22.866839745779107 64-65 23.315086228518513 26.7866649361776 31.075613082815845 18.822635752488043 66-67 19.554074204799537 34.939428391581615 26.558744537182832 18.947752866436012 68-69 19.6120428118368 35.36059859873825 26.46519507746053 18.562163511964428 70-71 18.950998326764363 36.26654221595013 26.355810651208017 18.426648806077484 72-73 18.86741876687081 35.924002754756 26.346766060437716 18.861812417935475 74-75 19.6047361204439 34.543972174870326 26.298541356322175 19.552750348363602 76-77 19.968022314932092 33.46472069381191 26.67737898940576 19.889878001850235 78-79 19.700259930368855 33.17746323120744 27.281588897237206 19.840687941186495 80-81 19.12707762639972 33.21614512419657 27.228558570695586 20.428218678708117 82-83 18.578565349515035 32.66016436995101 27.407140849746312 21.35412943078764 84-85 18.731112965743495 31.976500416973803 27.891192210671377 21.401194406611324 86-87 18.860369694881005 31.899290410573194 28.46285564674308 20.777484247802718 88-89 19.189095356703763 32.31750743730053 27.926792755718914 20.566604450276788 90-91 19.693061717490455 31.648228508196556 27.5628583719135 21.09585140239949 92-93 19.076455842668654 32.166459934828104 27.607851657706874 21.149232564796375 94-95 18.87554172900004 32.488705136493174 27.597245243033676 21.038507891473113 96-97 19.326172259175394 32.90520105621145 26.91134917650514 20.85727750810801 98-99 19.850489898598937 32.0646334210331 26.58642440761245 21.498452272755507 100-101 19.73034926048455 32.04793083140984 26.25014690936146 21.971572998744143 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 931.0 1 582.5 2 284.5 3 456.0 4 508.0 5 401.5 6 365.5 7 383.0 8 425.5 9 435.5 10 450.0 11 479.0 12 477.5 13 469.5 14 473.5 15 501.0 16 524.5 17 558.0 18 611.0 19 661.0 20 705.0 21 779.5 22 956.5 23 1255.0 24 1622.0 25 2214.0 26 3526.0 27 5870.5 28 8278.0 29 10383.0 30 12765.5 31 15070.0 32 18467.0 33 28040.0 34 45760.0 35 76990.5 36 117733.5 37 162234.5 38 212383.0 39 245404.0 40 256343.5 41 242813.0 42 218577.0 43 212230.0 44 201628.5 45 182042.5 46 171618.5 47 151823.0 48 117871.5 49 91646.5 50 72372.0 51 59090.0 52 49239.0 53 38150.5 54 28621.0 55 20571.5 56 14175.0 57 9328.5 58 6333.0 59 4561.0 60 3244.0 61 2359.0 62 1621.5 63 1085.5 64 790.5 65 577.5 66 405.0 67 313.5 68 222.5 69 144.5 70 115.5 71 90.5 72 58.5 73 44.5 74 38.0 75 27.5 76 21.0 77 15.0 78 10.0 79 9.5 80 6.0 81 3.0 82 2.0 83 3.0 84 3.0 85 1.0 86 1.5 87 1.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1650849212885031 2 0.0 3 2.866057661258734E-4 4 0.020317164309811918 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.209791599393925E-4 12-13 0.0 14-15 0.0 16-17 0.001066810351690751 18-19 0.0 20-21 0.0 22-23 4.7767627687645575E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 8.279722132525233E-4 38-39 6.846693301895866E-4 40-41 0.004872298024139848 42-43 0.0032959663104475444 44-45 0.0010508878091282025 46-47 0.004330931577013199 48-49 0.007897581111024067 50-51 0.005190748875390819 52-53 0.0014171062880668187 54-55 0.011066167080971225 56-57 0.017928782925429636 58-59 0.02190941856606677 60-61 0.011177624878909064 62-63 6.209791599393925E-4 64-65 0.013836689486854667 66-67 0.03264121225322448 68-69 0.01920258633043352 70-71 0.03421754396691678 72-73 0.02878795695308773 74-75 0.02807144253777305 76-77 0.036239706872360444 78-79 0.016400218839424982 80-81 0.012132977432661975 82-83 9.394300111903629E-4 84-85 0.010078969442093216 86-87 0.020380854480062112 88-89 0.019807642947810362 90-91 0.023676820790509655 92-93 0.04052287082168599 94-95 0.026511033366643292 96-97 0.027657456431146785 98-99 0.03448822719048011 100-101 0.01627283849892459 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3140202.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.30437095317543 #Duplication Level Percentage of deduplicated Percentage of total 1 85.52416067869653 31.904250154170754 2 6.255842476792889 4.667405367578273 3 2.3782685322488675 2.661594346598274 4 1.2394081168744706 1.8494136061704742 5 0.9033287393099786 1.6849055191941873 6 0.47620624733360883 1.065874470045153 7 0.3861243551658877 1.0082888325414756 8 0.28532224205805196 0.8515013407140226 9 0.24516838427977497 0.823126711784705 >10 1.8607470325848727 13.353079106403776 >50 0.22159670291783973 5.76986735508846 >100 0.18848579210246913 14.92227018785581 >500 0.027651520797493834 6.981551049552964 >1k 0.007263852771475696 4.20199699621632 >5k 3.4026085385450186E-4 0.7372537820936527 >10k+ 8.506521202330955E-5 7.5176211739914605 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 236902 7.544164356305741 TruSeq Adapter, Index 6 (97% over 36bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6914 0.22017691855492097 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5554 0.176867602784789 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT 5476 0.17438368614503144 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5289 0.1684286552266383 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 4913 0.1564549032196018 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4409 0.14040498031655288 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3571 0.11371879898172156 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3171 0.10098076493168275 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0038851003852618396 0.0 0.0 2 0.004426466832388489 0.0 0.0 3 0.00496783327951514 0.0 0.0 4 0.005413664471266498 0.0 0.0 5 0.0063690170250194095 0.0 0.0 6 0.007579130259773097 0.0 0.0 7 0.009203229601153046 0.0 0.0 8 0.010859174027658094 0.0 0.0 9 0.012324047943412558 0.0 0.0 10-11 0.02041269956518721 0.0 0.0 12-13 0.028214745420835984 0.0 0.0 14-15 0.038134489437303715 0.0 0.0 16-17 0.04869113515627339 0.0 0.0 18-19 0.06131771140837436 0.0 0.0 20-21 0.0705846311797776 0.0 0.0 22-23 0.08044068502599513 0.0 1.5922542562548523E-5 24-25 0.09298764856528338 0.0 3.1845085125097046E-5 26-27 0.10510470345538281 0.0 3.1845085125097046E-5 28-29 0.1180656531012973 0.0 3.1845085125097046E-5 30-31 0.21411042983859002 0.0 3.1845085125097046E-5 32-33 0.23293087514752236 0.0 3.1845085125097046E-5 34-35 0.2465605715810639 0.0 3.1845085125097046E-5 36-37 0.2604131836104811 0.0 3.1845085125097046E-5 38-39 0.27574659209821534 0.0 3.1845085125097046E-5 40-41 0.29123922601157504 0.0 3.1845085125097046E-5 42-43 0.30837188180887726 0.0 3.1845085125097046E-5 44-45 0.3277814611926239 0.0 3.1845085125097046E-5 46-47 0.34699997006562 0.0 3.1845085125097046E-5 48-49 0.3669509158964933 0.0 3.1845085125097046E-5 50-51 0.3895768488778747 0.0 3.1845085125097046E-5 52-53 0.4150529169779524 0.0 3.1845085125097046E-5 54-55 0.44083151338671844 0.0 3.1845085125097046E-5 56-57 0.4686800403286158 0.0 3.1845085125097046E-5 58-59 0.4991239417082086 0.0 3.1845085125097046E-5 60-61 0.5347585919631922 0.0 3.1845085125097046E-5 62-63 0.5709664537504275 0.0 3.1845085125097046E-5 64-65 0.6106135847311733 0.0 3.1845085125097046E-5 66-67 0.6513434486061724 0.0 3.1845085125097046E-5 68-69 0.6980124208570022 0.0 3.1845085125097046E-5 70-71 0.7485664934930938 0.0 3.1845085125097046E-5 72-73 0.801493024971005 0.0 3.1845085125097046E-5 74-75 0.8594033122709941 0.0 3.1845085125097046E-5 76-77 0.9203070375727421 0.0 3.1845085125097046E-5 78-79 0.9921177045298359 0.0 3.1845085125097046E-5 80-81 1.068434451032131 0.0 3.1845085125097046E-5 82-83 1.152378095421887 0.0 3.1845085125097046E-5 84-85 1.2409392771547818 0.0 3.1845085125097046E-5 86-87 1.3371432793176998 0.0 3.1845085125097046E-5 88-89 1.441435933102393 0.0 3.1845085125097046E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 33330 0.0 78.44383 3 CGGAAGA 33215 0.0 78.3865 4 GATCGGA 33605 0.0 77.95557 1 GAAGAGC 33435 0.0 77.59644 6 ATCGGAA 33750 0.0 77.49579 2 GGAAGAG 34420 0.0 75.679405 5 GCGACAT 30 1.301248E-4 63.336548 2 AGAGCAC 42120 0.0 61.66397 8 GAGCACA 41985 0.0 61.647327 9 AAGAGCA 42655 0.0 60.80148 7 GTCGACC 40 5.3456676E-4 47.57895 1 GGCGCGT 40 5.3456676E-4 47.57895 1 CGCGTGG 40 5.3456676E-4 47.57895 1 CGAGCGG 60 1.4761317E-6 47.578945 1 GCCGTCT 28090 0.0 44.981987 50-51 CGTCTTC 28085 0.0 44.923775 52-53 ATGCCGT 28165 0.0 44.79783 48-49 CTGCTTG 28245 0.0 44.685043 58-59 TCGTATG 28575 0.0 44.55362 44-45 TCTCGTA 27940 0.0 44.43576 42-43 >>END_MODULE