FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_41442-1.34100000035721.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_41442-1.34100000035721.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140202
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2369027.544164356305741TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69140.22017691855492097No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC55540.176867602784789No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT54760.17438368614503144TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC52890.1684286552266383No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG49130.1564549032196018No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG44090.14040498031655288No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA35710.11371879898172156No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31710.10098076493168275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG333300.078.443833
CGGAAGA332150.078.38654
GATCGGA336050.077.955571
GAAGAGC334350.077.596446
ATCGGAA337500.077.495792
GGAAGAG344200.075.6794055
GCGACAT301.301248E-463.3365482
AGAGCAC421200.061.663978
GAGCACA419850.061.6473279
AAGAGCA426550.060.801487
GTCGACC405.3456676E-447.578951
GGCGCGT405.3456676E-447.578951
CGCGTGG405.3456676E-447.578951
CGAGCGG601.4761317E-647.5789451
GCCGTCT280900.044.98198750-51
CGTCTTC280850.044.92377552-53
ATGCCGT281650.044.7978348-49
CTGCTTG282450.044.68504358-59
TCGTATG285750.044.5536244-45
TCTCGTA279400.044.4357642-43
Produced by FastQC (version 0.11.2)