##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_40825-1.341000000356f9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1864159 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.654779983896226 33.0 33.0 33.0 33.0 33.0 2 32.685104650408036 33.0 33.0 33.0 33.0 33.0 3 32.71777997477683 33.0 33.0 33.0 33.0 33.0 4 36.6446445823559 37.0 37.0 37.0 37.0 37.0 5 36.657596267271195 37.0 37.0 37.0 37.0 37.0 6 36.555039564758154 37.0 37.0 37.0 37.0 37.0 7 36.47484039719788 37.0 37.0 37.0 37.0 37.0 8 36.40767069761753 37.0 37.0 37.0 37.0 37.0 9 36.43838749806213 37.0 37.0 37.0 37.0 37.0 10-11 36.376340215614654 37.0 37.0 37.0 37.0 37.0 12-13 36.35302568074934 37.0 37.0 37.0 37.0 37.0 14-15 38.21790845094222 40.0 37.0 40.0 37.0 40.0 16-17 38.15741441583041 40.0 37.0 40.0 37.0 40.0 18-19 38.07251285968633 40.0 37.0 40.0 37.0 40.0 20-21 38.02486858685337 40.0 37.0 40.0 33.0 40.0 22-23 37.825486184386634 40.0 37.0 40.0 33.0 40.0 24-25 37.79405351152987 40.0 37.0 40.0 33.0 40.0 26-27 37.66229758298514 40.0 37.0 40.0 33.0 40.0 28-29 37.46938485397437 40.0 37.0 40.0 33.0 40.0 30-31 37.347955566021994 40.0 37.0 40.0 33.0 40.0 32-33 37.192274103228314 40.0 37.0 40.0 33.0 40.0 34-35 37.01383358393785 38.5 37.0 40.0 33.0 40.0 36-37 36.89155351018877 37.0 37.0 40.0 33.0 40.0 38-39 36.74997626275441 37.0 37.0 40.0 33.0 40.0 40-41 36.62373408062295 37.0 37.0 40.0 33.0 40.0 42-43 36.257714336598966 37.0 37.0 40.0 33.0 40.0 44-45 36.089453206512964 37.0 37.0 40.0 33.0 40.0 46-47 35.97495224388048 37.0 37.0 40.0 33.0 40.0 48-49 35.78620600495988 37.0 37.0 40.0 30.0 40.0 50-51 35.617445453955376 37.0 37.0 40.0 27.0 40.0 52-53 35.40618799147498 37.0 33.0 40.0 27.0 40.0 54-55 35.12274194422257 37.0 33.0 40.0 27.0 40.0 56-57 34.8629601874089 37.0 33.0 40.0 27.0 40.0 58-59 34.56234688135508 37.0 33.0 37.0 27.0 40.0 60-61 34.31598109388737 37.0 33.0 37.0 27.0 40.0 62-63 33.998024846593026 37.0 33.0 37.0 27.0 40.0 64-65 33.71047104887512 37.0 33.0 37.0 27.0 40.0 66-67 33.2808928852099 37.0 33.0 37.0 27.0 37.0 68-69 32.984198772744165 37.0 33.0 37.0 24.5 37.0 70-71 32.62361016415446 35.0 33.0 37.0 22.0 37.0 72-73 32.07532807019144 33.0 33.0 37.0 22.0 37.0 74-75 31.540396768730567 33.0 33.0 37.0 18.5 37.0 76-77 28.644896170337404 30.0 24.5 33.0 15.0 35.0 78-79 31.25822985056532 33.0 33.0 37.0 22.0 37.0 80-81 31.733461308826122 33.0 33.0 37.0 15.0 37.0 82-83 31.609735006509638 33.0 33.0 37.0 15.0 37.0 84-85 31.508916889600084 33.0 33.0 37.0 15.0 37.0 86-87 31.41590738772819 33.0 33.0 37.0 15.0 37.0 88-89 31.118530125380936 33.0 33.0 37.0 15.0 37.0 90-91 31.1053850556739 33.0 33.0 37.0 15.0 37.0 92-93 30.95466105627256 33.0 33.0 37.0 15.0 37.0 94-95 30.751319764033006 33.0 30.0 37.0 15.0 37.0 96-97 30.61377516617413 33.0 27.0 37.0 15.0 37.0 98-99 30.477918192600526 33.0 27.0 37.0 15.0 37.0 100-101 28.692064625388717 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.13592696081930455 2306 2 0.1871473768571903 2306 3 0.11251544006793068 2306 4 0.19297597363029695 2306 5 0.16245831403455213 2306 6 0.10049716809790255 2306 7 0.16091077577842583 2306 8 0.2743619676226672 2306 9 0.2120349441972209 2306 10-11 0.13883117647544196 2306 12-13 0.27361095253444745 2306 14-15 0.5024784288721165 2306 16-17 0.49619854786771356 2306 18-19 0.5850974359840748 2306 20-21 0.5415755932818413 2306 22-23 0.4840237797819782 2306 24-25 0.5028571294072677 2306 26-27 0.5550075253950908 2306 28-29 0.45277732555048544 2306 30-31 0.5645219089561024 2306 32-33 0.6429094523609891 2306 34-35 0.6623180649176987 2306 36-37 0.6172465815103791 2306 38-39 0.7076954549673076 2306 40-41 0.728354075915 2306 42-43 0.8155476250020044 2306 44-45 0.5412411637504917 2306 46-47 0.8976300501339409 2306 48-49 0.8783740384926375 2306 50-51 0.8364829803503611 2306 52-53 0.8219250064008534 2306 54-55 0.6874228353792304 2306 56-57 0.6018869106450069 2306 58-59 0.7392779967175755 2306 60-61 0.6049759777349308 2306 62-63 0.7773743476800163 2306 64-65 0.5123192202389859 2306 66-67 0.5401889050867723 2306 68-69 0.7042961263910215 2306 70-71 0.5718050340909784 2306 72-73 0.47529141743602565 2306 74-75 0.5850539279836262 2306 76-77 0.1622316949726894 2306 78-79 0.402926913803487 2306 80-81 0.6510910181861611 2306 82-83 0.5915784268923119 2306 84-85 0.6623883772649606 2306 86-87 0.6319402105487448 2306 88-89 0.8074206115707625 2306 90-91 0.6534013421080225 2306 92-93 0.7211579676256044 2306 94-95 0.6699899180082731 2306 96-97 0.5605846806070041 2306 98-99 0.5692490629311067 2306 100-101 0.335332182749422 2307 1 0.1603539704923591 2307 2 0.18119011147975783 2307 3 0.14990823481620197 2307 4 0.24959244731490315 2307 5 0.15181956359022308 2307 6 0.17671594244124833 2307 7 0.16987007181617741 2307 8 0.2577957310375041 2307 9 0.2855093019110839 2307 10-11 0.2661407217134055 2307 12-13 0.30247435943037715 2307 14-15 0.4229248527166831 2307 16-17 0.42194851194355465 2307 18-19 0.45863399921437775 2307 20-21 0.5433729829190455 2307 22-23 0.5718377852878263 2307 24-25 0.5319890221188288 2307 26-27 0.5918510586684249 2307 28-29 0.6591746146000474 2307 30-31 0.5559395802387144 2307 32-33 0.4274046801201479 2307 34-35 0.467766530767058 2307 36-37 0.3381813375664322 2307 38-39 0.652300639012438 2307 40-41 0.7861520289942661 2307 42-43 0.7832477887405318 2307 44-45 0.6420675139316216 2307 46-47 0.7129708947676292 2307 48-49 0.564617165633905 2307 50-51 0.7075906450531733 2307 52-53 0.7069119224442062 2307 54-55 0.6130922064417561 2307 56-57 0.6580595318984876 2307 58-59 0.5344975311404738 2307 60-61 0.5458753012263102 2307 62-63 0.7515399145632315 2307 64-65 0.6095250297426205 2307 66-67 0.5856667625699643 2307 68-69 0.591073105433999 2307 70-71 0.4925700484984503 2307 72-73 0.2322342337387937 2307 74-75 0.2944428485950219 2307 76-77 0.1411992819280634 2307 78-79 0.4418840638454604 2307 80-81 0.6809886626958139 2307 82-83 0.68089510213035 2307 84-85 0.6394727820205617 2307 86-87 0.5618968463080272 2307 88-89 0.7524606670074121 2307 90-91 0.6859687188125285 2307 92-93 0.6415668129097085 2307 94-95 0.4748718693329259 2307 96-97 0.5538311751654383 2307 98-99 0.47343389650771783 2307 100-101 0.5559419669775814 2201 1 0.07954942846641444 2201 2 0.08121765795750946 2201 3 0.08013522518787397 2201 4 0.10735648677476917 2201 5 0.15344739403703045 2201 6 0.13664783423720195 2201 7 0.16460666093365717 2201 8 0.17791186994570296 2201 9 0.19774511455067056 2201 10-11 0.16035854964591323 2201 12-13 0.22231642012876307 2201 14-15 0.30488080053736866 2201 16-17 0.3332183491050458 2201 18-19 0.3802263705039266 2201 20-21 0.3476233674604856 2201 22-23 0.46816180951393704 2201 24-25 0.4346043660615564 2201 26-27 0.4494126592481962 2201 28-29 0.4848493184291911 2201 30-31 0.3986709151890864 2201 32-33 0.5799724185728152 2201 34-35 0.48402912080152305 2201 36-37 0.4103092012908647 2201 38-39 0.5709502515129969 2201 40-41 0.432722862265976 2201 42-43 0.391042794878409 2201 44-45 0.43514077646989335 2201 46-47 0.4649241064281071 2201 48-49 0.5549352257702651 2201 50-51 0.42575643787408524 2201 52-53 0.49887685522340774 2201 54-55 0.5871675506802916 2201 56-57 0.5770959967795051 2201 58-59 0.7108365857601484 2201 60-61 0.5144265598600057 2201 62-63 0.5625262448547446 2201 64-65 0.6811269221301828 2201 66-67 0.41291660727438284 2201 68-69 0.4210660431826554 2201 70-71 0.3826882580143689 2201 72-73 0.4559079355417026 2201 74-75 0.2805942728773303 2201 76-77 0.07181774657243523 2201 78-79 0.406249736866922 2201 80-81 0.5984938946778335 2201 82-83 0.6100817045588798 2201 84-85 0.6209912215218409 2201 86-87 0.5425630088803182 2201 88-89 0.7241511148502866 2201 90-91 0.5320010092004708 2201 92-93 0.6631284023969357 2201 94-95 0.7157431699703771 2201 96-97 0.812156589656734 2201 98-99 0.6889827739425307 2201 100-101 0.4260362905544106 1101 1 0.135035398569741 1101 2 0.12376203056901858 1101 3 0.11533422661502613 1101 4 0.0799579713220453 1101 5 0.0903191260059728 1101 6 0.11191906622818237 1101 7 0.15591236927465957 1101 8 0.04346369987020893 1101 9 0.08867402415095427 1101 10-11 0.14123335547656524 1101 12-13 0.11820793943419261 1101 14-15 0.23158981492210984 1101 16-17 0.22254463226510524 1101 18-19 0.2637316793120519 1101 20-21 0.32848801640835745 1101 22-23 0.2903067962062593 1101 24-25 0.3059544794296585 1101 26-27 0.267166631021567 1101 28-29 0.304736883897867 1101 30-31 0.39184570606535374 1101 32-33 0.5307076472236929 1101 34-35 0.3735637281104971 1101 36-37 0.38117818096068135 1101 38-39 0.41169789317125094 1101 40-41 0.6161719408422144 1101 42-43 0.5825203490318813 1101 44-45 0.6875758350902288 1101 46-47 0.6845433573083 1101 48-49 0.662077588961381 1101 50-51 0.6770111370531424 1101 52-53 0.4540720359337982 1101 54-55 0.643761701525996 1101 56-57 0.5968308200194912 1101 58-59 0.7115158002830242 1101 60-61 0.5816917046366399 1101 62-63 0.7095188134742116 1101 64-65 0.6118252143043676 1101 66-67 0.533432127423481 1101 68-69 0.5770893772161685 1101 70-71 0.38167905285312287 1101 72-73 0.524704608579107 1101 74-75 0.4156058257912356 1101 76-77 0.11121218889906359 1101 78-79 0.7548959756651747 1101 80-81 0.8871691432621311 1101 82-83 0.9351211867393872 1101 84-85 0.9160875649575928 1101 86-87 0.7618382223693452 1101 88-89 1.1842661166958095 1101 90-91 1.1228901989059707 1101 92-93 0.964465393936905 1101 94-95 0.9315922724044832 1101 96-97 0.9164736106041182 1101 98-99 0.7471091013606674 1101 100-101 0.8808504801086379 2304 1 0.1315411570921654 2304 2 0.1053539330950386 2304 3 0.12334923556302613 2304 4 0.13362104008135844 2304 5 0.11582521812985647 2304 6 0.1741287238346132 2304 7 0.06619462105334861 2304 8 0.01718440062034432 2304 9 0.0588876226195012 2304 10-11 0.08911962387920624 2304 12-13 0.10651922233446243 2304 14-15 0.2057851962566346 2304 16-17 0.2744003683395917 2304 18-19 0.2504495865396663 2304 20-21 0.2051708331270916 2304 22-23 0.3503572027635542 2304 24-25 0.24014009434757355 2304 26-27 0.2682186837617735 2304 28-29 0.4196701869888244 2304 30-31 0.3777915753310026 2304 32-33 0.26768393969552307 2304 34-35 0.5094920988882592 2304 36-37 0.40320854337034007 2304 38-39 0.5043790824136991 2304 40-41 0.4844209930399259 2304 42-43 0.5698822707633653 2304 44-45 0.3644849629874187 2304 46-47 0.45731302108791283 2304 48-49 0.3286468252946264 2304 50-51 0.2619255698371319 2304 52-53 0.2834915927738777 2304 54-55 0.3969794357173342 2304 56-57 0.32893598554193204 2304 58-59 0.4741546579942053 2304 60-61 0.45674295299201617 2304 62-63 0.5795765623814333 2304 64-65 0.5150848825112107 2304 66-67 0.29354110909722664 2304 68-69 0.3900202141505318 2304 70-71 0.2284803760349874 2304 72-73 0.1940827614246956 2304 74-75 0.30414825241450316 2304 76-77 0.21532176139034576 2304 78-79 0.617674899223676 2304 80-81 0.7345334189104769 2304 82-83 0.7441452621644942 2304 84-85 0.6759005532784244 2304 86-87 0.7627630310941207 2304 88-89 0.9324757368889891 2304 90-91 0.7505958392648893 2304 92-93 0.917755733212708 2304 94-95 0.7346306178659496 2304 96-97 0.6454440525792862 2304 98-99 0.64755770958784 2304 100-101 0.7525105682791953 2202 1 0.06537188735810417 2202 2 0.0854531310688671 2202 3 0.03671704195109271 2202 4 0.052866854067573854 2202 5 0.05940795346122485 2202 6 0.10244376534298283 2202 7 0.10567752523233054 2202 8 0.10957746379749267 2202 9 0.1463724966873272 2202 10-11 0.07364350878140868 2202 12-13 0.11995544718320872 2202 14-15 0.0805920767969468 2202 16-17 0.029456474994994153 2202 18-19 0.13977032665505362 2202 20-21 0.11130000155258557 2202 22-23 0.18076894470812022 2202 24-25 0.14704297179233805 2202 26-27 0.26915596390811203 2202 28-29 0.24629059644428963 2202 30-31 0.31662300912866215 2202 32-33 0.3764773827798038 2202 34-35 0.34548073685083125 2202 36-37 0.3106249989774881 2202 38-39 0.2627447782065815 2202 40-41 0.3100620863282373 2202 42-43 0.45675173045099626 2202 44-45 0.3904123936895445 2202 46-47 0.3937779500312075 2202 48-49 0.3295311646783432 2202 50-51 0.31870726394153337 2202 52-53 0.3318068101651832 2202 54-55 0.4180880258397508 2202 56-57 0.3385353242221072 2202 58-59 0.45568044243208305 2202 60-61 0.422896920910965 2202 62-63 0.5512367213796026 2202 64-65 0.3825822442672333 2202 66-67 0.3938482006970574 2202 68-69 0.2722305349609968 2202 70-71 0.2657007221726033 2202 72-73 0.1086397123181797 2202 74-75 0.194273832828177 2202 76-77 -0.2307168273250042 2202 78-79 0.29331880663890075 2202 80-81 0.3532985448426551 2202 82-83 0.5222569830031034 2202 84-85 0.4499615143027711 2202 86-87 0.48944482369295983 2202 88-89 0.5546869371141199 2202 90-91 0.26233338675328355 2202 92-93 0.37204798029305053 2202 94-95 0.4077694246761361 2202 96-97 0.46549206861980963 2202 98-99 0.45188249501066124 2202 100-101 0.12142572235264382 1102 1 0.09503100605522263 1102 2 0.10072480906563896 1102 3 0.043387295125967285 1102 4 0.00735830350595279 1102 5 0.02648967694143778 1102 6 0.012518918992256545 1102 7 0.05471259150121455 1102 8 0.12604116670419785 1102 9 0.146805486041913 1102 10-11 0.09925052529565193 1102 12-13 0.1206608481385203 1102 14-15 0.16450463120590797 1102 16-17 0.17090477221871225 1102 18-19 0.2187685497032561 1102 20-21 0.15294131812714795 1102 22-23 0.20713727910110435 1102 24-25 0.2768000673727826 1102 26-27 0.25005063468296385 1102 28-29 0.1895971939891652 1102 30-31 0.20341243218990712 1102 32-33 0.3396926873884709 1102 34-35 0.2750316082093036 1102 36-37 0.23476187317255892 1102 38-39 0.32030758285599603 1102 40-41 0.256848901019886 1102 42-43 0.3395853979816863 1102 44-45 0.24681463688740735 1102 46-47 0.32859886915544223 1102 48-49 0.13713814858795814 1102 50-51 0.2514899052418045 1102 52-53 0.37906948928681317 1102 54-55 0.1886876248029239 1102 56-57 0.32627468288923467 1102 58-59 0.29849878579133815 1102 60-61 0.20791183752465514 1102 62-63 0.2609038282086402 1102 64-65 0.2758717243865547 1102 66-67 0.39697971143213096 1102 68-69 0.3669070156183025 1102 70-71 0.33033612100862086 1102 72-73 0.28834741189920976 1102 74-75 0.3144009908464689 1102 76-77 0.2675864282463074 1102 78-79 0.3979631717454346 1102 80-81 0.5061838906961604 1102 82-83 0.6517866613585284 1102 84-85 0.7469103739561831 1102 86-87 0.5139199708213091 1102 88-89 0.7833212926022881 1102 90-91 0.5081787430402684 1102 92-93 0.5063971105648086 1102 94-95 0.538969926787285 1102 96-97 0.5840644753298392 1102 98-99 0.18656500752268457 1102 100-101 0.2725368878047867 2305 1 0.08674008437846936 2305 2 0.051670592537163884 2305 3 0.0711360454670853 2305 4 0.1072732280726072 2305 5 0.04788983946819059 2305 6 0.04825786206009042 2305 7 0.04385185925950452 2305 8 0.06190028033435624 2305 9 0.025615349709440238 2305 10-11 0.06267111439764506 2305 12-13 -0.037452540592674666 2305 14-15 -0.04476611016658438 2305 16-17 -0.0012676949264971427 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-1.2850229838444243 1213 70-71 -1.1653537657464383 1213 72-73 -0.791405886565471 1213 74-75 -0.79638806518415 1213 76-77 -0.5954109851740554 1213 78-79 -1.1207515804571635 1213 80-81 -1.2985930946294992 1213 82-83 -1.635560973068138 1213 84-85 -1.6183990135455133 1213 86-87 -1.272282549921215 1213 88-89 -0.9818376588138449 1213 90-91 -1.1299005190383262 1213 92-93 -1.1977066647498589 1213 94-95 -1.0696962662843 1213 96-97 -1.1422493033022683 1213 98-99 -1.2720013212313326 1213 100-101 -0.9589820305563457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 16.0 10 18.0 11 15.0 12 37.0 13 184.0 14 702.0 15 2276.0 16 4750.0 17 7095.0 18 6715.0 19 6598.0 20 6999.0 21 7949.0 22 9167.0 23 11017.0 24 13582.0 25 16794.0 26 21037.0 27 28393.0 28 41729.0 29 58609.0 30 53860.0 31 52397.0 32 69220.0 33 99344.0 34 153179.0 35 264356.0 36 445312.0 37 427043.0 38 55747.0 39 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.1683860004094 23.229299359635263 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24.448504660814876 28.827208408724793 22.842096623732203 20-21 25.34298308245166 25.09968302060071 29.050821308697376 20.50651258825025 22-23 23.796683797794223 29.62196453558873 25.742422620920358 20.838929045696688 24-25 23.825703708750165 24.47358299372532 28.389343398283085 23.311369899241427 26-27 22.74111274842972 24.794907515936142 26.539018399181614 25.924961336452522 28-29 24.81893980073588 27.04855111607969 25.967554269780635 22.164954813403792 30-31 22.490812210760993 25.335875319648167 28.363755452190503 23.80955701740034 32-33 23.006969899026853 27.138886758050145 25.849324011524764 24.004819331398235 34-35 23.367480992769394 26.814397269760786 28.226320823491985 21.59180091397783 36-37 22.7686321043179 27.643540105184783 28.560312807781358 21.027514982715964 38-39 22.741381049144167 26.09395820503522 27.25502481562623 23.909635930194383 40-41 25.38447198554785 25.458395752333924 24.832377322355637 24.32475493976259 42-43 23.299039142156158 27.355627910395036 27.235570971863066 22.10976197558574 44-45 22.77358937936125 25.204785681792824 27.190549657108033 24.831075281737892 46-47 25.139793223780472 25.106881766865108 25.376932743412677 24.37639226594174 48-49 22.95909280138024 27.09662000060085 27.19232692282932 22.75196027518959 50-51 25.027124735156637 24.869244694482273 26.30263774470266 23.80099282565843 52-53 22.855415511191136 24.94797174984705 24.53367694285516 27.66293579610666 54-55 25.12378803871838 24.94435942590355 27.24036969429538 22.69148284108269 56-57 23.01139381634902 24.996512535022198 26.89594692185611 25.096146726772677 58-59 22.57292909694977 25.174435325326446 27.1567256595383 25.095909918185487 60-61 24.508518441422435 25.14262675728397 27.267730568747332 23.081124232546262 62-63 22.720703806024193 24.988439771477616 27.379985516187 24.910870906311185 64-65 25.23281151542156 25.610379953221752 26.917155717271392 22.2396528140853 66-67 23.07397992722371 29.400934468604827 24.779516602728492 22.74556900144297 68-69 22.69101979344339 28.450626918460625 25.52466762778604 23.333685660309936 70-71 22.751010001950643 28.875121982370146 25.232943732084994 23.14092428359422 72-73 22.963760508288175 29.026821103478582 25.245165442907595 22.764252945325644 74-75 23.583186529734636 28.362347948944006 25.353200604635862 22.701264916685492 76-77 23.561248707033602 27.68843769411266 25.220109501340925 23.530204097512808 78-79 23.390741232552962 27.876363772932983 25.42625365170574 23.30664134280832 80-81 23.581930124435203 28.160586541855913 25.335686410843255 22.921796922865624 82-83 23.084010251334725 27.852731610561932 25.484517721907736 23.578740416195608 84-85 22.88076215737146 27.93024787925999 25.349576066593276 23.839413896775273 86-87 22.555045775571735 28.21047373410947 25.46957318162624 23.764907308692557 88-89 22.57517322490747 28.386054657193355 25.648446529116914 23.39032558878226 90-91 23.035974427008856 27.796516042142315 25.260196868032956 23.907312662815873 92-93 22.72600058979367 27.537461155593096 24.8814308104672 24.85510744414603 94-95 22.932727418093858 28.05204582247413 24.50012797625276 24.51509878317925 96-97 22.723051955107763 28.758756151004082 24.300842688565282 24.217349205322872 98-99 22.889000448893366 27.665274193890276 24.798527908798178 24.647197448418183 100-101 23.522506383296072 27.833451100384206 24.392104725221838 24.251937791097884 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3220.0 1 1934.0 2 712.5 3 1967.0 4 2464.5 5 1447.0 6 1278.0 7 1512.0 8 1587.5 9 1531.0 10 1365.0 11 1230.5 12 1219.5 13 1170.0 14 1073.0 15 1054.0 16 1099.5 17 1157.0 18 1229.5 19 1286.0 20 1324.5 21 1424.5 22 1571.0 23 1660.0 24 1808.5 25 1987.0 26 2198.0 27 2559.0 28 2920.0 29 3410.0 30 4160.5 31 5097.5 32 6378.0 33 8093.5 34 10853.0 35 16131.5 36 23248.5 37 32068.0 38 41525.5 39 47843.0 40 55095.0 41 59521.5 42 64245.0 43 75058.5 44 80402.0 45 83479.5 46 93494.0 47 99643.0 48 96032.5 49 92573.0 50 89791.5 51 87414.5 52 84819.5 53 81618.0 54 76264.0 55 67031.0 56 58064.0 57 51758.5 58 48041.0 59 41568.0 60 32121.0 61 23776.5 62 16657.0 63 11564.5 64 8718.0 65 7210.5 66 6824.5 67 6319.0 68 5324.0 69 4138.0 70 2833.0 71 1966.0 72 1727.0 73 1468.5 74 906.5 75 547.0 76 424.5 77 337.5 78 274.5 79 229.5 80 173.0 81 126.0 82 102.0 83 81.0 84 54.5 85 39.0 86 30.5 87 19.5 88 17.5 89 17.0 90 9.0 91 8.0 92 5.5 93 2.0 94 1.5 95 1.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15427868545547885 2 0.0 3 2.6821746428282135E-4 4 0.020867318721203502 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.2914794285251416E-4 12-13 0.0 14-15 0.0 16-17 0.0010996916035595676 18-19 0.0 20-21 0.0 22-23 4.5596968928079636E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 5.90078421422207E-4 38-39 5.90078421422207E-4 40-41 0.004103727203527167 42-43 0.0023334919392605457 44-45 8.046523928484641E-4 46-47 0.003567292274961524 48-49 0.0072418715356361765 50-51 0.004586518639236246 52-53 0.0014751960535555177 54-55 0.010004511417749237 56-57 0.018292431064088416 58-59 0.023120345421179203 60-61 0.010889629049882547 62-63 4.8279143570907846E-4 64-65 0.013866842903421866 66-67 0.03433183542820113 68-69 0.019311657428363136 70-71 0.03607524894603947 72-73 0.030603612674669916 74-75 0.029933069013962865 76-77 0.03816734516744548 78-79 0.01630762182839554 80-81 0.011640637949874447 82-83 6.169001678504892E-4 84-85 0.010031333164177519 86-87 0.021028249199773193 88-89 0.021296466664056015 90-91 0.025427015614011464 92-93 0.04267339856739688 94-95 0.028538338199692193 96-97 0.02899430788897299 98-99 0.03687990133888794 100-101 0.016629482785534926 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1864159.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.95088216145471 #Duplication Level Percentage of deduplicated Percentage of total 1 82.14790785142901 38.56916741342483 2 8.151710909247896 7.654600366286854 3 2.957000073380592 4.165012859901152 4 1.6999949792043982 3.192650557747614 5 1.0343585296180515 2.428202271839634 6 0.708743454072294 1.9965678236910396 7 0.4789480477751983 1.5740923346786477 8 0.34206649962697355 1.284825913229386 9 0.2646822369518462 1.1184358065620725 >10 1.9489650927589006 17.70899108874325 >50 0.16798244740079116 5.355097153737123 >100 0.09140095081721711 7.709233031150801 >500 0.004424469324120759 1.3240935688071642 >1k 0.001588095801120328 1.4631135221660607 >5k 0.0 0.0 >10k+ 2.2636259154097487E-4 4.455916288034279 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 69685 3.7381467997096816 TruSeq Adapter, Index 27 (97% over 39bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14168 0.7600210067918026 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 4132 0.22165491248332359 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4129 0.2214939820047539 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 3826 0.2052400036692149 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 2212 0.11865940619872016 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1906 0.1022444973846115 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0026821746428282137 0.0 0.0 2 0.002950392107111035 0.0 0.0 3 0.0032186095713938566 0.0 0.0 4 0.003540470528533242 0.0 0.0 5 0.004827914357090785 0.0 0.0 6 0.005686210242795813 0.0 0.0 7 0.0067590800999270985 0.0 0.0 8 0.007510088999918998 0.0 0.0 9 0.009441254742755312 0.0 0.0 10-11 0.01872157900694093 0.0 0.0 12-13 0.03301756985321531 0.0 0.0 14-15 0.050317596299457285 0.0 0.0 16-17 0.07097034104923454 0.0 0.0 18-19 0.09240091644543197 0.0 0.0 20-21 0.0994013922632136 0.0 0.0 22-23 0.10634822458813867 0.0 0.0 24-25 0.11431428327733847 0.0 0.0 26-27 0.12289724213438875 0.0 0.0 28-29 0.13292857529856628 0.0 0.0 30-31 0.19032711265509006 0.0 0.0 32-33 0.20226278981567558 0.0 0.0 34-35 0.21065799644772792 0.0 0.0 36-37 0.21902638133335195 0.0 0.0 38-39 0.22709972700826486 0.0 0.0 40-41 0.2359240815831697 0.0 0.0 42-43 0.24512394060807047 0.0 0.0 44-45 0.254779769322252 0.0 0.0 46-47 0.2646769937542881 0.0 0.0 48-49 0.27588848376131003 0.0 0.0 50-51 0.2868049345576209 0.0 0.0 52-53 0.2992234031539155 0.0 0.0 54-55 0.31376078971804444 0.0 0.0 56-57 0.32773491960717943 0.0 0.0 58-59 0.3438816109570053 0.0 0.0 60-61 0.3627104769496593 0.0 0.0 62-63 0.3833632216994366 0.0 0.0 64-65 0.404606044870636 0.0 0.0 66-67 0.4289601906275162 0.0 0.0 68-69 0.45516503688794785 0.0 0.0 70-71 0.48456167097334507 0.0 0.0 72-73 0.5175524190801322 0.0 0.0 74-75 0.5576509299904139 0.0 5.364349285656427E-5 76-77 0.6037038686077744 0.0 5.364349285656427E-5 78-79 0.6536996039500922 0.0 5.364349285656427E-5 80-81 0.7086037188887858 0.0 5.364349285656427E-5 82-83 0.7708301706024003 0.0 5.364349285656427E-5 84-85 0.84000345464094 0.0 5.364349285656427E-5 86-87 0.9156139578222673 0.0 5.364349285656427E-5 88-89 1.0009071114642045 0.0 5.364349285656427E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 10945 0.0 71.081696 4 ATCGGAA 11080 0.0 70.472824 2 GATCGGA 11125 0.0 70.28392 1 GAAGAGC 11230 0.0 69.4799 6 TCGGAAG 11275 0.0 69.21188 3 GGAAGAG 13615 0.0 57.378555 5 GAGCACA 14665 0.0 53.14078 9 AAGAGCA 14930 0.0 52.165745 7 AGAGCAC 15470 0.0 50.529026 8 TATGCCG 8940 0.0 43.270298 48-49 GTATGCC 9010 0.0 43.013195 46-47 CTCGTAT 8625 0.0 42.78566 44-45 GATCTCG 8565 0.0 42.77926 40-41 TCGTATG 9100 0.0 42.561695 44-45 TACTCGA 8865 0.0 42.53696 36-37 GCCGTCT 9175 0.0 42.427094 50-51 TCGATCT 8640 0.0 42.270493 38-39 ATGCCGT 9180 0.0 42.21666 48-49 CTCGATC 8575 0.0 42.203217 38-39 TTACTCG 9120 0.0 42.1276 34-35 >>END_MODULE