FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_40825-1.341000000356f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_40825-1.341000000356f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1864159
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT696853.7381467997096816TruSeq Adapter, Index 27 (97% over 39bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141680.7600210067918026No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA41320.22165491248332359No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41290.2214939820047539No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC38260.2052400036692149No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT22120.11865940619872016TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19060.1022444973846115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA109450.071.0816964
ATCGGAA110800.070.4728242
GATCGGA111250.070.283921
GAAGAGC112300.069.47996
TCGGAAG112750.069.211883
GGAAGAG136150.057.3785555
GAGCACA146650.053.140789
AAGAGCA149300.052.1657457
AGAGCAC154700.050.5290268
TATGCCG89400.043.27029848-49
GTATGCC90100.043.01319546-47
CTCGTAT86250.042.7856644-45
GATCTCG85650.042.7792640-41
TCGTATG91000.042.56169544-45
TACTCGA88650.042.5369636-37
GCCGTCT91750.042.42709450-51
TCGATCT86400.042.27049338-39
ATGCCGT91800.042.2166648-49
CTCGATC85750.042.20321738-39
TTACTCG91200.042.127634-35