##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_40255-1.341000000357f8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7760650 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66815460045228 33.0 33.0 33.0 33.0 33.0 2 32.70949082873213 33.0 33.0 33.0 33.0 33.0 3 32.74059904776018 33.0 33.0 33.0 33.0 33.0 4 36.667456591909186 37.0 37.0 37.0 37.0 37.0 5 36.669811033869586 37.0 37.0 37.0 37.0 37.0 6 36.56963798135465 37.0 37.0 37.0 37.0 37.0 7 36.502862131393634 37.0 37.0 37.0 37.0 37.0 8 36.44924870983745 37.0 37.0 37.0 37.0 37.0 9 36.47060671464375 37.0 37.0 37.0 37.0 37.0 10-11 36.425517063648016 37.0 37.0 37.0 37.0 37.0 12-13 36.40101615199758 37.0 37.0 37.0 37.0 37.0 14-15 38.311300277682925 40.0 37.0 40.0 37.0 40.0 16-17 38.28832262761495 40.0 37.0 40.0 37.0 40.0 18-19 38.27789238014857 40.0 37.0 40.0 37.0 40.0 20-21 38.227395385695786 40.0 37.0 40.0 37.0 40.0 22-23 38.08977534098304 40.0 37.0 40.0 37.0 40.0 24-25 38.09457506781004 40.0 37.0 40.0 37.0 40.0 26-27 37.99789592366619 40.0 37.0 40.0 35.0 40.0 28-29 37.861332427051856 40.0 37.0 40.0 33.0 40.0 30-31 37.80410377996688 40.0 37.0 40.0 33.0 40.0 32-33 37.67285961871751 40.0 37.0 40.0 33.0 40.0 34-35 37.557980710378644 40.0 37.0 40.0 33.0 40.0 36-37 37.41318523577277 40.0 37.0 40.0 33.0 40.0 38-39 37.35606495589931 40.0 37.0 40.0 33.0 40.0 40-41 37.3029338393047 40.0 37.0 40.0 33.0 40.0 42-43 37.08917616436768 40.0 37.0 40.0 33.0 40.0 44-45 37.01222036813927 40.0 37.0 40.0 33.0 40.0 46-47 36.89321197322389 37.0 37.0 40.0 33.0 40.0 48-49 36.75364170526953 37.0 37.0 40.0 33.0 40.0 50-51 36.625700231295056 37.0 37.0 40.0 33.0 40.0 52-53 36.48391139917403 37.0 37.0 40.0 33.0 40.0 54-55 36.2943928665769 37.0 37.0 40.0 33.0 40.0 56-57 36.092246847880006 37.0 37.0 40.0 33.0 40.0 58-59 35.84488535109817 37.0 37.0 40.0 30.0 40.0 60-61 35.574528615515455 37.0 35.0 40.0 27.0 40.0 62-63 35.32973172350255 37.0 33.0 40.0 27.0 40.0 64-65 35.057235283127056 37.0 33.0 37.0 27.0 40.0 66-67 34.66788638838241 37.0 33.0 37.0 27.0 40.0 68-69 34.382696939045054 37.0 33.0 37.0 27.0 40.0 70-71 34.03668371850296 37.0 33.0 37.0 27.0 40.0 72-73 33.57795319979641 37.0 33.0 37.0 27.0 37.0 74-75 33.04489269584378 37.0 33.0 37.0 24.5 37.0 76-77 30.304950551822337 33.0 30.0 35.0 22.0 37.0 78-79 32.713233105474416 35.0 33.0 37.0 27.0 37.0 80-81 33.0999666265068 37.0 33.0 37.0 27.0 37.0 82-83 32.96755355543672 37.0 33.0 37.0 27.0 37.0 84-85 32.83063699561248 37.0 33.0 37.0 27.0 37.0 86-87 32.72461681689034 37.0 33.0 37.0 27.0 37.0 88-89 32.47201039861352 37.0 33.0 37.0 22.0 37.0 90-91 32.45800776996772 35.0 33.0 37.0 22.0 37.0 92-93 32.299365194925684 33.0 33.0 37.0 22.0 37.0 94-95 32.12410616378783 33.0 33.0 37.0 22.0 37.0 96-97 32.01497393903861 33.0 33.0 37.0 22.0 37.0 98-99 31.881026911405616 33.0 33.0 37.0 22.0 37.0 100-101 30.26124313040789 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.1512555830457316 2306 2 0.1385462729882363 2306 3 0.1100426270545043 2306 4 0.17157978923073358 2306 5 0.12508288070890927 2306 6 0.15756974597285733 2306 7 0.1902388288872885 2306 8 0.21396925833683866 2306 9 0.17234060839771104 2306 10-11 0.16845731732380642 2306 12-13 0.21139781196499996 2306 14-15 0.4176397551633997 2306 16-17 0.34069132028823645 2306 18-19 0.40251481801010414 2306 20-21 0.40985130330998487 2306 22-23 0.4617716216706711 2306 24-25 0.4354087257090882 2306 26-27 0.41954629071810956 2306 28-29 0.4618770431095385 2306 30-31 0.4775829148276358 2306 32-33 0.49927569989895915 2306 34-35 0.5186782034496105 2306 36-37 0.41869880424788875 2306 38-39 0.534653229999293 2306 40-41 0.618566638447902 2306 42-43 0.5528590091173484 2306 44-45 0.5133963807375039 2306 46-47 0.6317297100341577 2306 48-49 0.5739398064286974 2306 50-51 0.5993838678276902 2306 52-53 0.5487793215217707 2306 54-55 0.5444278753347618 2306 56-57 0.5621378954210456 2306 58-59 0.6068573642050623 2306 60-61 0.5894693362103567 2306 62-63 0.564706973467132 2306 64-65 0.5323085269653305 2306 66-67 0.4666024140541296 2306 68-69 0.4774188425566308 2306 70-71 0.38182880534855457 2306 72-73 0.3961841803602866 2306 74-75 0.4303599069453199 2306 76-77 -0.0015582530767765945 2306 78-79 0.28480280221475596 2306 80-81 0.4852862286500468 2306 82-83 0.5818813698053802 2306 84-85 0.5841277471775328 2306 86-87 0.5199836637314874 2306 88-89 0.7292443762876459 2306 90-91 0.5258632703787782 2306 92-93 0.541093055388707 2306 94-95 0.4912522497245888 2306 96-97 0.5553629458978762 2306 98-99 0.4569963336906646 2306 100-101 0.20320359695233492 2307 1 0.15288626048477028 2307 2 0.13242528166747292 2307 3 0.09705283227784633 2307 4 0.1399459169168864 2307 5 0.1661706522138502 2307 6 0.17257295752089163 2307 7 0.19272079891676697 2307 8 0.2556844754411003 2307 9 0.20414539906388285 2307 10-11 0.21245077176818228 2307 12-13 0.23562958576474102 2307 14-15 0.41242759681652785 2307 16-17 0.3975436846481344 2307 18-19 0.37717762675133315 2307 20-21 0.3415889505213059 2307 22-23 0.3570989948797916 2307 24-25 0.3895690834499277 2307 26-27 0.5045252955731385 2307 28-29 0.5172403283291374 2307 30-31 0.42916309011508247 2307 32-33 0.4714999776979738 2307 34-35 0.47611437211430996 2307 36-37 0.4587985804079935 2307 38-39 0.540983026928572 2307 40-41 0.4774710376195159 2307 42-43 0.49332667124448193 2307 44-45 0.5294746431796824 2307 46-47 0.5619634495723247 2307 48-49 0.37891072086458877 2307 50-51 0.41051493793904825 2307 52-53 0.3668386865952087 2307 54-55 0.4049389708731539 2307 56-57 0.4830462970194347 2307 58-59 0.5280858740957299 2307 60-61 0.5203520723854282 2307 62-63 0.48162240237109444 2307 64-65 0.4863020282366506 2307 66-67 0.37198216674967455 2307 68-69 0.3468808910552923 2307 70-71 0.3099978313246652 2307 72-73 0.32708619717556076 2307 74-75 0.2338698916208486 2307 76-77 -0.019698337871862037 2307 78-79 0.405564653210142 2307 80-81 0.5334988973396904 2307 82-83 0.6069053171764338 2307 84-85 0.5066646622424571 2307 86-87 0.5251892592109542 2307 88-89 0.6676581092402429 2307 90-91 0.5130518082208795 2307 92-93 0.5926579303263466 2307 94-95 0.4807730887442645 2307 96-97 0.5720698955629473 2307 98-99 0.4979747004221373 2307 100-101 0.40357350853951246 2201 1 0.12203063001661008 2201 2 0.11468478341654276 2201 3 0.10084256821418336 2201 4 0.1492518355768695 2201 5 0.14405588859887075 2201 6 0.11052743115893549 2201 7 0.14029457341798945 2201 8 0.16605311585563953 2201 9 0.1419953896563939 2201 10-11 0.152250749988049 2201 12-13 0.20047952475160002 2201 14-15 0.31868021767767374 2201 16-17 0.3042029426792965 2201 18-19 0.2577852990732197 2201 20-21 0.32158926577765357 2201 22-23 0.32895087085245933 2201 24-25 0.36045628171999766 2201 26-27 0.3444137129013214 2201 28-29 0.29166275495555993 2201 30-31 0.2422864618345102 2201 32-33 0.3685178301088783 2201 34-35 0.34769704527789 2201 36-37 0.30885516925543044 2201 38-39 0.4332273729466962 2201 40-41 0.40451247133841406 2201 42-43 0.4715316987062508 2201 44-45 0.4431895020542811 2201 46-47 0.44770536587437704 2201 48-49 0.4303788950678751 2201 50-51 0.4722657899845686 2201 52-53 0.38524913803225047 2201 54-55 0.4243082433398371 2201 56-57 0.4433792518889845 2201 58-59 0.49014445581837407 2201 60-61 0.4660725612964711 2201 62-63 0.46613471520604577 2201 64-65 0.4511121920474608 2201 66-67 0.39196968634693974 2201 68-69 0.32955087864517196 2201 70-71 0.21305038281602862 2201 72-73 0.29633417961866115 2201 74-75 0.29816304172978647 2201 76-77 -0.08048255441267216 2201 78-79 0.35208797484997945 2201 80-81 0.4623647348844173 2201 82-83 0.5478726474498856 2201 84-85 0.5449551884280481 2201 86-87 0.4864018194250761 2201 88-89 0.7764442699287386 2201 90-91 0.4758663525553217 2201 92-93 0.5710002716689075 2201 94-95 0.5639129287586968 2201 96-97 0.4921066814931194 2201 98-99 0.49768824218118723 2201 100-101 0.4153280017622727 1101 1 0.12230343319080106 1101 2 0.09000150961752951 1101 3 0.10411679794013651 1101 4 0.09742414305509328 1101 5 0.12801688419379076 1101 6 0.0914410301161439 1101 7 0.14359548590940108 1101 8 0.08943660174418255 1101 9 0.12850515140710428 1101 10-11 0.09053250768534582 1101 12-13 0.15796077463622282 1101 14-15 0.2591638064161472 1101 16-17 0.2753106549096387 1101 18-19 0.2783486296408597 1101 20-21 0.24106056127501319 1101 22-23 0.23930405600552973 1101 24-25 0.2646340632474278 1101 26-27 0.2027406230441713 1101 28-29 0.17949752012592768 1101 30-31 0.2354574192003014 1101 32-33 0.2869282638156392 1101 34-35 0.2559153133759011 1101 36-37 0.24562781245136023 1101 38-39 0.3379230722765385 1101 40-41 0.3367591909556822 1101 42-43 0.2701356576003562 1101 44-45 0.33824165946532503 1101 46-47 0.34741502662106427 1101 48-49 0.3703578186413381 1101 50-51 0.31306735919149986 1101 52-53 0.23102606679979232 1101 54-55 0.2868701698378686 1101 56-57 0.2826998388672308 1101 58-59 0.3585534281852034 1101 60-61 0.3551146476497067 1101 62-63 0.3139101002779938 1101 64-65 0.310900856262073 1101 66-67 0.2802170581832897 1101 68-69 0.3176190677243369 1101 70-71 0.25851260877633564 1101 72-73 0.2300007655073415 1101 74-75 0.17766716353666112 1101 76-77 -0.20988695603952578 1101 78-79 0.3302477467531659 1101 80-81 0.3992361220616374 1101 82-83 0.5007896061873183 1101 84-85 0.4753673348015326 1101 86-87 0.36672497197192655 1101 88-89 0.5499469053103425 1101 90-91 0.36615273731629827 1101 92-93 0.3100172963382235 1101 94-95 0.36331793267059 1101 96-97 0.35271413979345567 1101 98-99 0.3067495673407912 1101 100-101 0.3258003719065421 2304 1 0.09150550952743686 2304 2 0.13382074326958815 2304 3 0.08838318522205668 2304 4 0.11238720463706642 2304 5 0.08936840790353529 2304 6 0.15849357625810967 2304 7 0.13490532966652324 2304 8 0.118950584094911 2304 9 0.09440068028491311 2304 10-11 0.11377244590045876 2304 12-13 0.17625103730482294 2304 14-15 0.23832155251007947 2304 16-17 0.24073078800388714 2304 18-19 0.28427570318226003 2304 20-21 0.2762910951014135 2304 22-23 0.26165324828821923 2304 24-25 0.22130145805368073 2304 26-27 0.230459292681374 2304 28-29 0.265488666500616 2304 30-31 0.269715058454409 2304 32-33 0.2869007256452534 2304 34-35 0.32101772224113034 2304 36-37 0.3134167975973128 2304 38-39 0.33733602267056284 2304 40-41 0.32247754380039595 2304 42-43 0.3577256225254217 2304 44-45 0.36745732889132654 2304 46-47 0.3999410843559801 2304 48-49 0.3040885574511094 2304 50-51 0.24246725483632048 2304 52-53 0.34200251234668855 2304 54-55 0.3714914475261537 2304 56-57 0.2881038424616804 2304 58-59 0.42111896074857214 2304 60-61 0.3309721096656659 2304 62-63 0.3274616235736332 2304 64-65 0.3459964427008728 2304 66-67 0.36851043701326347 2304 68-69 0.2516443407249227 2304 70-71 0.2491242667398339 2304 72-73 0.10945842919381477 2304 74-75 0.23328929842815427 2304 76-77 -0.011947608342172344 2304 78-79 0.19741520781667532 2304 80-81 0.3169284130303609 2304 82-83 0.3858365349566313 2304 84-85 0.39869532269301544 2304 86-87 0.3496295139065566 2304 88-89 0.5675838093574228 2304 90-91 0.38732896918355664 2304 92-93 0.41232558512044903 2304 94-95 0.4284315224470987 2304 96-97 0.39915115606375906 2304 98-99 0.42701113854870343 2304 100-101 0.5741612813701487 2202 1 0.04033336331076498 2202 2 0.042658146763301374 2202 3 0.03844661928868476 2202 4 0.01999617859680569 2202 5 0.04387476399701029 2202 6 0.033001039564382495 2202 7 0.0403961824741117 2202 8 0.04726466807238694 2202 9 0.0929445801190596 2202 10-11 0.13624609769588147 2202 12-13 0.057353163845405675 2202 14-15 0.0588488164719152 2202 16-17 0.08976670552575428 2202 18-19 0.035564395079546784 2202 20-21 0.15079181132968955 2202 22-23 0.18662775932689613 2202 24-25 0.12111421222245866 2202 26-27 0.07754668867833203 2202 28-29 0.13316751020138895 2202 30-31 0.1963830284028063 2202 32-33 0.13310131339071063 2202 34-35 0.11229375039201273 2202 36-37 0.09348269982581314 2202 38-39 0.17491816978684938 2202 40-41 0.12426321277605723 2202 42-43 0.2615697269632662 2202 44-45 0.25845038343770455 2202 46-47 0.33707763598784624 2202 48-49 0.27068729199914543 2202 50-51 0.24917788707789867 2202 52-53 0.2549404669094457 2202 54-55 0.20758283640928 2202 56-57 0.2258833271925269 2202 58-59 0.29035955116980006 2202 60-61 0.2756743270899662 2202 62-63 0.30735435080022455 2202 64-65 0.2895470434791463 2202 66-67 0.23946559258819633 2202 68-69 0.21563778048908944 2202 70-71 0.17783562097643113 2202 72-73 0.2050339607136209 2202 74-75 0.11918773241404779 2202 76-77 -0.23343145802880727 2202 78-79 0.18910615243066076 2202 80-81 0.25617339770237635 2202 82-83 0.3501439156832902 2202 84-85 0.4194115048387488 2202 86-87 0.4454282180062492 2202 88-89 0.5966977969151159 2202 90-91 0.40278012379890527 2202 92-93 0.4650896380729037 2202 94-95 0.4044639983825178 2202 96-97 0.36239226779767364 2202 98-99 0.3932529699709413 2202 100-101 0.37066450728077527 1102 1 0.08821255748176071 1102 2 0.06447987829238144 1102 3 0.07032346942325063 1102 4 0.057141485845065176 1102 5 0.06649931777868545 1102 6 0.009347748325531313 1102 7 0.016295662991865356 1102 8 0.061525757118666036 1102 9 0.05578659134732078 1102 10-11 0.03635670308010219 1102 12-13 0.07567499943456824 1102 14-15 0.14330106414411858 1102 16-17 0.15622003624097403 1102 18-19 0.0987378645142627 1102 20-21 0.08215925284767422 1102 22-23 0.13016450327415185 1102 24-25 0.08349007519246499 1102 26-27 0.12081369457575875 1102 28-29 0.11487967950381517 1102 30-31 0.1319596618776302 1102 32-33 0.15821292869465253 1102 34-35 0.12338543013650138 1102 36-37 0.08609995661796432 1102 38-39 0.14664205415795095 1102 40-41 0.15395234670470614 1102 42-43 0.1981991314276783 1102 44-45 0.05657551223442425 1102 46-47 0.2071915804990212 1102 48-49 0.11380457410322009 1102 50-51 0.06543600926131177 1102 52-53 0.018761614474819055 1102 54-55 0.09450153812808537 1102 56-57 0.08113278758858655 1102 58-59 0.1255302224877184 1102 60-61 0.0945127302202593 1102 62-63 0.16927025286818065 1102 64-65 0.03634196739256623 1102 66-67 0.19394705378605437 1102 68-69 0.07247820959501894 1102 70-71 -0.12222671280229491 1102 72-73 -0.01228102327339542 1102 74-75 0.030577939312081526 1102 76-77 0.023031184401830274 1102 78-79 0.2036461327231649 1102 80-81 0.2589340835478069 1102 82-83 0.32266832122409284 1102 84-85 0.24653549383241113 1102 86-87 0.20849604216873985 1102 88-89 0.5323589056995743 1102 90-91 0.24791985322224264 1102 92-93 0.3086913236928197 1102 94-95 0.35197184891030986 1102 96-97 0.32528444263292755 1102 98-99 0.1941326483920527 1102 100-101 0.2541658452326345 2305 1 0.05108625929305077 2305 2 0.04987325957843325 2305 3 0.062330593607811124 2305 4 0.06635738872110863 2305 5 0.029406926612949746 2305 6 0.0331260127181352 2305 7 0.022704516953965026 2305 8 0.049316836065919745 2305 9 0.05078205943627978 2305 10-11 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Count 8 9.0 9 28.0 10 51.0 11 50.0 12 109.0 13 320.0 14 1057.0 15 3063.0 16 6535.0 17 11017.0 18 12989.0 19 14865.0 20 18187.0 21 22105.0 22 27451.0 23 33887.0 24 41950.0 25 52664.0 26 67285.0 27 88747.0 28 128866.0 29 177044.0 30 169130.0 31 169490.0 32 223875.0 33 315934.0 34 487872.0 35 858716.0 36 1681351.0 37 2511733.0 38 633928.0 39 342.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.18883135009265 27.397386864160843 20.34814363277069 15.065638152975817 2 31.617441837990373 25.564972006210816 28.35299878231849 14.464587373480315 3 35.03212495267783 19.977040535605724 27.01162044235046 17.97921406936598 4 30.989768869028534 19.67005298577251 28.31251295259325 21.027665192605706 5 31.73993157789618 18.255223467106493 31.25940481789541 18.74544013710192 6 29.02352251422239 18.83884726150516 33.311707137933034 18.825923086339415 7 31.92246783452417 21.9037838325398 25.87702061038702 20.29672772254901 8 29.904453879507514 28.262529556158313 22.213667669589533 19.619348894744643 9 29.3370142964829 25.538157241983594 24.760503308356903 20.3643251531766 10-11 24.46477549379729 28.66786627345816 28.99045092978331 17.876907302961236 12-13 22.714289395862462 27.957297391326758 28.592437489127843 20.73597572368294 14-15 22.776507122470413 28.5234677507683 28.48892811813443 20.211097008626854 16-17 23.6432321761793 27.558019900268647 28.970284767887218 19.828463155664835 18-19 21.442205227654902 27.360092260313245 30.739602997171627 20.458099514860226 20-21 22.686211850811468 27.82556229181834 30.406847364589307 19.081378492780885 22-23 21.346098215154342 30.503851821021915 28.796467805708698 19.353582158115046 24-25 21.409057231030907 26.94826464278121 30.64246551513082 21.000212611057066 26-27 21.15335699973585 27.169502554554064 29.184043862305348 22.49309658340474 28-29 22.312570467679897 29.311178831670027 28.5854921945971 19.79075850605297 30-31 20.220690277231935 28.797156166042793 29.945436271446336 21.03671728527894 32-33 20.094818088691024 30.146733843170352 28.726021660556782 21.032426407581838 34-35 22.050195537744905 29.892393034088638 28.828738572155682 19.22867285601077 36-37 21.684378514356695 30.48498645880413 29.077223956593436 18.75341107024574 38-39 20.40171296232465 28.68085046615651 31.273790874947537 19.643645696571305 40-41 20.60423479286104 28.084478854224177 28.1736768793165 23.137609473598282 42-43 20.877072430572667 29.647790293301373 29.77233988747304 19.702797388652915 44-45 20.308067438572678 28.170881958659372 29.998388650014075 21.52266195275388 46-47 21.95733475424106 28.129230870337125 28.641234437210688 21.27219993821113 48-49 20.88001833497712 29.658661823731 29.41435869171407 20.046961149577808 50-51 22.038822465619337 28.360984293158637 28.86388052754493 20.736312713677098 52-53 20.57234172602842 28.20378379670431 27.82738594750867 23.3964885297586 54-55 22.360163321356424 28.010726058625142 29.457478475425923 20.171632144592508 56-57 20.952944648647534 27.651794781909384 29.444521056403012 21.95073951304007 58-59 20.719337346621888 27.852501818095117 29.73837796594386 21.689782869339137 60-61 21.77691354448141 27.989644235319343 29.565052213116527 20.668390007082714 62-63 20.18567625756473 28.150113821674587 29.567477636568128 22.09673228419255 64-65 21.98184053437742 28.226068200354028 29.53768682902306 20.254404436245498 66-67 21.121919818178608 30.656277056946667 27.925018268373456 20.296784856501265 68-69 20.976088472561305 30.39952257109462 28.087290459502768 20.537098496841306 70-71 20.717013253214176 31.098297018921073 27.974123004414214 20.210566723450533 72-73 20.797569612656535 31.321701314964635 27.772005084381252 20.108723987997582 74-75 21.620626526063212 30.550052682149442 27.474973481696203 20.354347310091146 76-77 21.698356546826357 29.60352963515012 27.723032385281638 20.975081432741884 78-79 21.33517155697951 29.590552759091427 28.300494793185344 20.773780890743723 80-81 20.84352172766644 30.100806145022595 28.177206795258442 20.878465332052517 82-83 20.164270076174137 30.11211091551417 28.334297716528294 21.389321291783396 84-85 20.409592656747765 29.83772263611652 28.479336181462187 21.273348525673526 86-87 20.25617519400406 30.053839502502065 28.944577430017603 20.745407873476267 88-89 20.238545558776035 30.4726910779079 28.79714463976834 20.491618723547724 90-91 20.795902336555173 29.554489750846972 28.58316291635462 21.066444996243234 92-93 20.399740244254193 29.987769565070266 28.083805067170708 21.528685123504836 94-95 20.264434347206706 30.358006547057254 27.702836166642875 21.674722939093165 96-97 20.49102511033126 30.733801900032613 27.421643842520186 21.353529147115932 98-99 20.66188372772761 29.990928464416218 27.749604993437238 21.59758281441894 100-101 20.913259392881347 30.137510991638816 27.301310542294566 21.647919073185275 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3292.0 1 2062.0 2 991.0 3 1610.0 4 1690.5 5 1235.0 6 1205.0 7 1336.5 8 1418.5 9 1352.5 10 1279.5 11 1266.0 12 1273.5 13 1284.0 14 1317.0 15 1405.0 16 1537.5 17 1727.0 18 1969.0 19 2345.0 20 2858.5 21 3670.5 22 4879.5 23 6695.0 24 9011.0 25 12006.5 26 16344.5 27 21663.5 28 27675.5 29 34917.0 30 43549.5 31 53712.5 32 69196.5 33 92349.0 34 124580.5 35 176330.5 36 248164.0 37 336465.5 38 421237.5 39 462122.5 40 489114.0 41 485220.5 42 456780.5 43 467101.5 44 465055.0 45 449343.0 46 459848.5 47 427964.5 48 339848.0 49 267695.0 50 223533.0 51 197951.0 52 178508.5 53 152526.5 54 119527.5 55 87590.0 56 65990.5 57 54462.0 58 46454.5 59 36251.5 60 26277.0 61 19382.5 62 13923.5 63 9569.5 64 6816.5 65 4904.5 66 3512.5 67 2580.5 68 1795.0 69 1171.5 70 755.0 71 505.0 72 373.0 73 268.0 74 183.5 75 124.0 76 80.0 77 60.0 78 50.5 79 42.0 80 34.5 81 24.0 82 18.0 83 17.5 84 10.5 85 7.5 86 5.5 87 2.5 88 2.5 89 2.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1628858407478755 2 0.0 3 2.0616829775856404E-4 4 0.02043643251531766 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.63604208410378E-4 12-13 0.0 14-15 0.0 16-17 0.0013078801389058907 18-19 0.0 20-21 0.0 22-23 4.509931513468588E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.342000992184933E-4 38-39 7.602455979847049E-4 40-41 0.005006023979950133 42-43 0.0037045866003491976 44-45 9.857421736581343E-4 46-47 0.004542145309993364 48-49 0.00796969326022949 50-51 0.005167092962574012 52-53 0.0014367353250049932 54-55 0.01103644668938813 56-57 0.019199422728766274 58-59 0.02374156803875964 60-61 0.01177736400945797 62-63 5.862910967509165E-4 64-65 0.014373796009354886 66-67 0.034881098877027054 68-69 0.020475089071147392 70-71 0.037000766688357285 72-73 0.030673977050891355 74-75 0.030016815601785936 76-77 0.03892715172053887 78-79 0.017363236326854065 80-81 0.012937060684349895 82-83 9.857421736581343E-4 84-85 0.010546796982211542 86-87 0.02142861744828075 88-89 0.020990509815543804 90-91 0.025571311681366896 92-93 0.04332755632582322 94-95 0.028380354738327333 96-97 0.029759105229587725 98-99 0.03694922461391765 100-101 0.017305251493109467 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7760650.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.36684389737688 #Duplication Level Percentage of deduplicated Percentage of total 1 88.35536645080329 36.5498265146591 2 5.70586455436427 4.720672166399254 3 1.7947195981021058 2.2272565636275834 4 0.8718722404399579 1.4426641147494392 5 0.5052080359400584 1.0449430979216383 6 0.4516593793440447 1.1210233824066735 7 0.290526585206895 0.8412717528804113 8 0.22501358590028098 0.7446481506180741 9 0.19723151394859129 0.7342960724239219 >10 1.2012519537471231 10.275662262750835 >50 0.1883252689173096 5.450927258576667 >100 0.17619578656906273 14.565062661162795 >500 0.021675794849782642 6.326016634887282 >1k 0.014746114131134865 10.282689493966693 >5k 2.1843436441558873E-4 0.6131243664980757 >10k+ 1.2470337176725382E-4 3.0599155064716275 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 197286 2.542132424474754 TruSeq Adapter, Index 7 (97% over 38bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16931 0.21816471558439046 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 12571 0.1619838544451818 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10480 0.13504023503185944 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 9933 0.12799185635223853 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 9851 0.1269352438262259 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0022807367939541145 0.0 0.0 2 0.00253844716615232 0.0 0.0 3 0.0027317299453009734 0.0 0.0 4 0.002950783761669448 0.0 0.0 5 0.003427547950236127 0.0 0.0 6 0.004058938362121729 0.0 0.0 7 0.00458724462512805 0.0 0.0 8 0.005347490223112755 0.0 0.0 9 0.005824254411679434 0.0 0.0 10-11 0.010289086610013336 0.0 0.0 12-13 0.01383904698704361 0.0 0.0 14-15 0.018664673706455 0.0 0.0 16-17 0.02459845502631867 0.0 0.0 18-19 0.0316726047431594 0.0 1.2885518609910253E-5 20-21 0.036884797020868096 0.0 1.2885518609910253E-5 22-23 0.04277992178490204 0.0 1.2885518609910253E-5 24-25 0.0492033528119423 0.0 1.2885518609910253E-5 26-27 0.0561228763054641 0.0 1.2885518609910253E-5 28-29 0.06394438610167963 0.0 1.2885518609910253E-5 30-31 0.11218776777718362 0.0 1.2885518609910253E-5 32-33 0.12352702415390464 0.0 1.9328277914865378E-5 34-35 0.13235360440169314 0.0 2.5771037219820505E-5 36-37 0.14139923846585015 0.0 2.5771037219820505E-5 38-39 0.15177852370613287 0.0 2.5771037219820505E-5 40-41 0.16333683389922238 0.0 2.5771037219820505E-5 42-43 0.17606772628581369 0.0 2.5771037219820505E-5 44-45 0.18952665047386497 0.0 2.5771037219820505E-5 46-47 0.2035589802400572 0.0 2.5771037219820505E-5 48-49 0.21927287018484276 0.0 2.5771037219820505E-5 50-51 0.23650725132559774 0.0 2.5771037219820505E-5 52-53 0.25508816916108834 0.0 2.5771037219820505E-5 54-55 0.2745388595027478 0.0 2.5771037219820505E-5 56-57 0.295194345834434 0.0 2.5771037219820505E-5 58-59 0.31854290555559134 0.0 3.8656555829730756E-5 60-61 0.34361168201117176 0.0 3.8656555829730756E-5 62-63 0.37185673880409503 0.0 3.8656555829730756E-5 64-65 0.4015643019592431 0.0 5.154207443964101E-5 66-67 0.4330951659976935 0.0 5.154207443964101E-5 68-69 0.4685689987307764 0.0 5.154207443964101E-5 70-71 0.5090359699251996 0.0 5.154207443964101E-5 72-73 0.5525761373080863 0.0 5.154207443964101E-5 74-75 0.6010192445220439 0.0 5.154207443964101E-5 76-77 0.6546294446985755 0.0 6.442759304955127E-5 78-79 0.71571968842816 0.0 7.087035235450639E-5 80-81 0.7830980652393807 0.0 7.731311165946151E-5 82-83 0.8560494288493876 0.0 7.731311165946151E-5 84-85 0.9371573257394676 0.0 7.731311165946151E-5 86-87 1.0287798058152346 0.0 8.375587096441664E-5 88-89 1.1284106357070605 0.0 9.019863026937176E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 34485 0.0 63.65086 4 TCGGAAG 34680 0.0 63.361446 3 GATCGGA 35350 0.0 62.33637 1 GAAGAGC 35745 0.0 61.340992 6 ATCGGAA 36055 0.0 61.01096 2 GGAAGAG 39890 0.0 55.193207 5 GCCGTCT 25270 0.0 43.35576 50-51 ATGCCGT 25220 0.0 43.172882 48-49 TGCCGTC 25325 0.0 42.99901 50-51 TATGCCG 25200 0.0 42.96213 48-49 TCGTATG 25465 0.0 42.850082 44-45 CGTATGC 25395 0.0 42.6035 46-47 TCTCGTA 25330 0.0 42.262962 42-43 CTCGTAT 25300 0.0 42.200306 44-45 CGTCTTC 25940 0.0 42.182896 52-53 CATCTCG 25340 0.0 41.85147 40-41 GTATGCC 26235 0.0 41.61053 46-47 ATCTCGT 25450 0.0 41.57848 42-43 GTCACGA 26830 0.0 40.20617 28-29 CGAGATT 26675 0.0 40.15572 32-33 >>END_MODULE