FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_40255-1.341000000357f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_40255-1.341000000357f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7760650
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1972862.542132424474754TruSeq Adapter, Index 7 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169310.21816471558439046No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA125710.1619838544451818No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC104800.13504023503185944No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG99330.12799185635223853No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC98510.1269352438262259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA344850.063.650864
TCGGAAG346800.063.3614463
GATCGGA353500.062.336371
GAAGAGC357450.061.3409926
ATCGGAA360550.061.010962
GGAAGAG398900.055.1932075
GCCGTCT252700.043.3557650-51
ATGCCGT252200.043.17288248-49
TGCCGTC253250.042.9990150-51
TATGCCG252000.042.9621348-49
TCGTATG254650.042.85008244-45
CGTATGC253950.042.603546-47
TCTCGTA253300.042.26296242-43
CTCGTAT253000.042.20030644-45
CGTCTTC259400.042.18289652-53
CATCTCG253400.041.8514740-41
GTATGCC262350.041.6105346-47
ATCTCGT254500.041.5784842-43
GTCACGA268300.040.2061728-29
CGAGATT266750.040.1557232-33