##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_35011-1.341000000358a7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6133638 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.682330127731696 33.0 33.0 33.0 33.0 33.0 2 32.724485044601586 33.0 33.0 33.0 33.0 33.0 3 32.75727423105178 33.0 33.0 33.0 33.0 33.0 4 36.68181852271034 37.0 37.0 37.0 37.0 37.0 5 36.689282119355596 37.0 37.0 37.0 37.0 37.0 6 36.58372160208998 37.0 37.0 37.0 37.0 37.0 7 36.51931855124153 37.0 37.0 37.0 37.0 37.0 8 36.473993574449615 37.0 37.0 37.0 37.0 37.0 9 36.50256144884977 37.0 37.0 37.0 37.0 37.0 10-11 36.44914999874463 37.0 37.0 37.0 37.0 37.0 12-13 36.422701421244625 37.0 37.0 37.0 37.0 37.0 14-15 38.33889463316876 40.0 37.0 40.0 37.0 40.0 16-17 38.34487142866925 40.0 37.0 40.0 37.0 40.0 18-19 38.3377588471964 40.0 37.0 40.0 37.0 40.0 20-21 38.316429743652954 40.0 37.0 40.0 37.0 40.0 22-23 38.185678303806 40.0 37.0 40.0 37.0 40.0 24-25 38.19176115382095 40.0 37.0 40.0 37.0 40.0 26-27 38.12520848149174 40.0 37.0 40.0 37.0 40.0 28-29 37.995497859508504 40.0 37.0 40.0 37.0 40.0 30-31 37.961773013014465 40.0 37.0 40.0 35.0 40.0 32-33 37.842440652676274 40.0 37.0 40.0 33.0 40.0 34-35 37.71968226687 40.0 37.0 40.0 33.0 40.0 36-37 37.51870325571871 40.0 37.0 40.0 33.0 40.0 38-39 37.566437895421934 40.0 37.0 40.0 33.0 40.0 40-41 37.41818134686136 40.0 37.0 40.0 33.0 40.0 42-43 37.255955845454196 40.0 37.0 40.0 33.0 40.0 44-45 37.247585527544985 40.0 37.0 40.0 33.0 40.0 46-47 37.11729466264556 40.0 37.0 40.0 33.0 40.0 48-49 36.995487262208826 38.5 37.0 40.0 33.0 40.0 50-51 36.90183175140105 37.0 37.0 40.0 33.0 40.0 52-53 36.78045582409656 37.0 37.0 40.0 33.0 40.0 54-55 36.60038300271388 37.0 37.0 40.0 33.0 40.0 56-57 36.4296855308383 37.0 37.0 40.0 33.0 40.0 58-59 36.15430124829669 37.0 37.0 40.0 33.0 40.0 60-61 35.93409906160096 37.0 37.0 40.0 33.0 40.0 62-63 35.70415396213471 37.0 37.0 40.0 30.0 40.0 64-65 35.434505590320136 37.0 33.0 40.0 27.0 40.0 66-67 35.03239749395057 37.0 33.0 37.0 27.0 40.0 68-69 34.77753553437617 37.0 33.0 37.0 27.0 40.0 70-71 34.41211406672517 37.0 33.0 37.0 27.0 40.0 72-73 33.90410919261946 37.0 33.0 37.0 27.0 40.0 74-75 33.32281893714627 37.0 33.0 37.0 24.5 37.0 76-77 30.63031898850242 33.0 30.0 35.0 22.0 37.0 78-79 32.949664701438195 35.0 33.0 37.0 27.0 37.0 80-81 33.247542160134 37.0 33.0 37.0 27.0 37.0 82-83 33.10599606628236 37.0 33.0 37.0 27.0 37.0 84-85 32.95986117863493 37.0 33.0 37.0 27.0 37.0 86-87 32.850074784328655 37.0 33.0 37.0 27.0 37.0 88-89 32.56695969015452 37.0 33.0 37.0 22.0 37.0 90-91 32.54672708105695 37.0 33.0 37.0 22.0 37.0 92-93 32.39914313495514 35.0 33.0 37.0 22.0 37.0 94-95 32.218376271961276 33.0 33.0 37.0 22.0 37.0 96-97 32.107177094572585 33.0 33.0 37.0 22.0 37.0 98-99 31.996029436363866 33.0 33.0 37.0 22.0 37.0 100-101 30.41846486538658 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.12060007562785557 2306 2 0.1298922845110866 2306 3 0.1163968227436385 2306 4 0.16868134330860585 2306 5 0.16645045582841078 2306 6 0.17722878662891617 2306 7 0.21646593980105422 2306 8 0.22309596551834687 2306 9 0.15750225635922988 2306 10-11 0.200132911547378 2306 12-13 0.22406016434560172 2306 14-15 0.40596340190240454 2306 16-17 0.38278677764230196 2306 18-19 0.386884340558332 2306 20-21 0.38229951817521624 2306 22-23 0.37110206556979364 2306 24-25 0.3885670568094355 2306 26-27 0.37374197796469133 2306 28-29 0.4103183638813519 2306 30-31 0.4445253976764434 2306 32-33 0.451185456882385 2306 34-35 0.5090570640776448 2306 36-37 0.48796773302185414 2306 38-39 0.5413331503987067 2306 40-41 0.6137640709770764 2306 42-43 0.5778832893087582 2306 44-45 0.4727792705835512 2306 46-47 0.5690342395211303 2306 48-49 0.47662881300042415 2306 50-51 0.42685760803261985 2306 52-53 0.4891104415156846 2306 54-55 0.46581620419588177 2306 56-57 0.5239706339358605 2306 58-59 0.5951929678329648 2306 60-61 0.47901562882777426 2306 62-63 0.5104436648155755 2306 64-65 0.4003428811682923 2306 66-67 0.3395782486557479 2306 68-69 0.49672880747340287 2306 70-71 0.25732498611146326 2306 72-73 0.236620505864245 2306 74-75 0.29602166115913064 2306 76-77 -0.02156088982264137 2306 78-79 0.25295604758640167 2306 80-81 0.3266053442691401 2306 82-83 0.48205053611463455 2306 84-85 0.44376654517852643 2306 86-87 0.3576191047475916 2306 88-89 0.5708800623077792 2306 90-91 0.4059827816469195 2306 92-93 0.42579261871570395 2306 94-95 0.390280990080484 2306 96-97 0.39920376512890243 2306 98-99 0.3026448980753358 2306 100-101 0.1881632867856915 2307 1 0.148691502551209 2307 2 0.10304607374698094 2307 3 0.10548081043148017 2307 4 0.1666491516567632 2307 5 0.15297680816690473 2307 6 0.1577408877246782 2307 7 0.19940505035494027 2307 8 0.1627589843204973 2307 9 0.17148678706156772 2307 10-11 0.17810543810832513 2307 12-13 0.1839181139391286 2307 14-15 0.38018655936681256 2307 16-17 0.3085009673522592 2307 18-19 0.25661259530262015 2307 20-21 0.29890650715042 2307 22-23 0.30579143006013254 2307 24-25 0.3350518132719529 2307 26-27 0.27146155511762515 2307 28-29 0.30232211325044744 2307 30-31 0.29877518434182804 2307 32-33 0.309940576738299 2307 34-35 0.3693744443915037 2307 36-37 0.34918360385763236 2307 38-39 0.39626133765995064 2307 40-41 0.3260541052576187 2307 42-43 0.3159357454745262 2307 44-45 0.3334583572103611 2307 46-47 0.3545351156106378 2307 48-49 0.2655348526628103 2307 50-51 0.289401018740449 2307 52-53 0.29031022562594444 2307 54-55 0.280701745533797 2307 56-57 0.30267336342895845 2307 58-59 0.4210781052526684 2307 60-61 0.2993789872152419 2307 62-63 0.30799971459341435 2307 64-65 0.39824600659925125 2307 66-67 0.3310310731781314 2307 68-69 0.29130678294009726 2307 70-71 0.1382805572077217 2307 72-73 0.17378749209532884 2307 74-75 0.11813712856671543 2307 76-77 -0.20499639276382453 2307 78-79 0.1460337153892226 2307 80-81 0.3381470447998325 2307 82-83 0.3862887372575088 2307 84-85 0.4022195314082069 2307 86-87 0.30732911666913054 2307 88-89 0.6205478623255587 2307 90-91 0.44161116848717086 2307 92-93 0.36754390169856777 2307 94-95 0.33767865771529415 2307 96-97 0.38321959424280294 2307 98-99 0.3531805272322117 2307 100-101 0.3810914278448543 2201 1 0.14359610880570273 2201 2 0.11679207289037663 2201 3 0.08946045892552235 2201 4 0.15248992853584298 2201 5 0.15204471351520255 2201 6 0.17156966670409446 2201 7 0.1664776263045411 2201 8 0.17746476142126966 2201 9 0.11892361679640828 2201 10-11 0.18204146401449606 2201 12-13 0.18751505311080763 2201 14-15 0.32736156804879357 2201 16-17 0.29767125816876217 2201 18-19 0.23126465778766914 2201 20-21 0.30022630312220855 2201 22-23 0.341744751955531 2201 24-25 0.364602661765268 2201 26-27 0.33867592360853394 2201 28-29 0.36498061986889496 2201 30-31 0.362080850124201 2201 32-33 0.41549272252083114 2201 34-35 0.3995063272096431 2201 36-37 0.4715209256125945 2201 38-39 0.4551600012314907 2201 40-41 0.509409326407841 2201 42-43 0.4851340166858549 2201 44-45 0.4803786569733006 2201 46-47 0.5714755656691679 2201 48-49 0.4518098748263384 2201 50-51 0.4845820754369825 2201 52-53 0.48341411161933934 2201 54-55 0.5064320727752332 2201 56-57 0.5106146235055178 2201 58-59 0.573098142564362 2201 60-61 0.5145763117925455 2201 62-63 0.4497003833365554 2201 64-65 0.5306677939460016 2201 66-67 0.4081200993132015 2201 68-69 0.35443302489084516 2201 70-71 0.20970245056127368 2201 72-73 0.2905311881227277 2201 74-75 0.2573695042602253 2201 76-77 0.025921470868212282 2201 78-79 0.36776841884362454 2201 80-81 0.44254322126268164 2201 82-83 0.4666546565424383 2201 84-85 0.529430195287631 2201 86-87 0.499631829790637 2201 88-89 0.6739773400045976 2201 90-91 0.44811692537687975 2201 92-93 0.491616449064594 2201 94-95 0.5472458731190954 2201 96-97 0.46402525045979814 2201 98-99 0.5609477876045048 2201 100-101 0.4923174134211763 1101 1 0.10444198988064812 1101 2 0.1057675484153151 1101 3 0.08956075138418385 1101 4 0.12867909166319436 1101 5 0.12209810115125208 1101 6 0.09866331244884208 1101 7 0.12552780059623814 1101 8 0.07436972638601702 1101 9 0.0947972206428247 1101 10-11 0.11794722163943305 1101 12-13 0.14833549504020738 1101 14-15 0.26359559435990576 1101 16-17 0.2645514225084895 1101 18-19 0.28506601039374146 1101 20-21 0.22754823012019898 1101 22-23 0.23965467501177784 1101 24-25 0.2755654490334152 1101 26-27 0.2747025324260477 1101 28-29 0.24325778576506707 1101 30-31 0.3315464328514466 1101 32-33 0.32561365912062 1101 34-35 0.23744443057594822 1101 36-37 0.36110661526966226 1101 38-39 0.3494020102691948 1101 40-41 0.4054291880563099 1101 42-43 0.3763267445471854 1101 44-45 0.2967723866204537 1101 46-47 0.42142794098583636 1101 48-49 0.36322405949867687 1101 50-51 0.3724078886660749 1101 52-53 0.26661087476767165 1101 54-55 0.32018110736881056 1101 56-57 0.4097420059089458 1101 58-59 0.45906803693191733 1101 60-61 0.34104896582952193 1101 62-63 0.36276418780449404 1101 64-65 0.2608929151327217 1101 66-67 0.28233503426748285 1101 68-69 0.29486949230250303 1101 70-71 0.1215410276935387 1101 72-73 0.10450344054249427 1101 74-75 0.15366675630379945 1101 76-77 -0.22250050904157348 1101 78-79 0.17628125981663345 1101 80-81 0.421039415965069 1101 82-83 0.46208691663259316 1101 84-85 0.423035595300405 1101 86-87 0.3585498059327463 1101 88-89 0.571831951729223 1101 90-91 0.457018348789596 1101 92-93 0.4666506221134057 1101 94-95 0.5274059424860127 1101 96-97 0.4900838422414324 1101 98-99 0.3116515429592326 1101 100-101 0.31211586055554363 2304 1 0.11634896893479407 2304 2 0.08688282985399809 2304 3 0.08786026562501803 2304 4 0.11157921487409794 2304 5 0.09644912587596366 2304 6 0.05251940474235539 2304 7 0.13762532929371218 2304 8 0.10542917217796344 2304 9 0.1266867880315914 2304 10-11 0.1254857643034839 2304 12-13 0.17129760348771583 2304 14-15 0.2834284233830502 2304 16-17 0.2262669754931892 2304 18-19 0.22395939096644213 2304 20-21 0.25887589881944706 2304 22-23 0.313331070738613 2304 24-25 0.24938641855058563 2304 26-27 0.23259394615249107 2304 28-29 0.2722629983093583 2304 30-31 0.2943154135021544 2304 32-33 0.24165685816356586 2304 34-35 0.29187688258979705 2304 36-37 0.33069013758237276 2304 38-39 0.39152494802030446 2304 40-41 0.4286366958145109 2304 42-43 0.4506476056066262 2304 44-45 0.3513310074763254 2304 46-47 0.3550814619912117 2304 48-49 0.41461023694428434 2304 50-51 0.3886867367792348 2304 52-53 0.33986001276607425 2304 54-55 0.3348301315778883 2304 56-57 0.3279829529749989 2304 58-59 0.38577452986172034 2304 60-61 0.33830560603542636 2304 62-63 0.38703114865818833 2304 64-65 0.28856420713817954 2304 66-67 0.23638646003016817 2304 68-69 0.3938543717610443 2304 70-71 0.24363050787790996 2304 72-73 0.0763673140391532 2304 74-75 0.1219194986045764 2304 76-77 0.09867230246765502 2304 78-79 0.24015815262314533 2304 80-81 0.31721487501150136 2304 82-83 0.49861423885138123 2304 84-85 0.37980934663056587 2304 86-87 0.290111334332245 2304 88-89 0.5615808702559164 2304 90-91 0.34241909827998995 2304 92-93 0.3620795487032282 2304 94-95 0.42972390695716456 2304 96-97 0.4019288506660814 2304 98-99 0.4298904085132733 2304 100-101 0.4720817748834314 2202 1 0.09349665895828707 2202 2 0.09680379908574821 2202 3 0.04218392720559194 2202 4 0.06677907226338675 2202 5 0.07667661176460427 2202 6 -3.6244365094972864E-4 2202 7 0.022741386272329578 2202 8 0.03790327197608434 2202 9 0.06075688356335718 2202 10-11 0.08132411636278647 2202 12-13 0.09457016841277976 2202 14-15 0.10949476514773693 2202 16-17 0.11959807023180957 2202 18-19 0.053700720283600845 2202 20-21 0.11181021261472068 2202 22-23 0.15580133342055547 2202 24-25 0.17898811485769528 2202 26-27 0.12322845814449579 2202 28-29 0.1303748599833412 2202 30-31 0.13555534638950206 2202 32-33 0.08931448916273155 2202 34-35 0.14765106448079734 2202 36-37 0.07940022049080397 2202 38-39 0.16325731258981335 2202 40-41 0.17221432842482187 2202 42-43 0.30300832935599686 2202 44-45 0.22241016831969063 2202 46-47 0.28884253773434665 2202 48-49 0.20706472378134322 2202 50-51 0.27932272765283983 2202 52-53 0.21523328082124493 2202 54-55 0.24890719347283863 2202 56-57 0.25166520133765147 2202 58-59 0.28631558783566646 2202 60-61 0.22151377281178952 2202 62-63 0.25504337492135676 2202 64-65 0.1991265158099509 2202 66-67 0.07590690226163588 2202 68-69 0.15007694058437693 2202 70-71 0.06659526935291638 2202 72-73 0.07447378062011722 2202 74-75 0.07869660814942847 2202 76-77 -0.15827199381168455 2202 78-79 0.17211813335580928 2202 80-81 0.2051566260857527 2202 82-83 0.3042384488544272 2202 84-85 0.3112138494628027 2202 86-87 0.28152348547487094 2202 88-89 0.4348620359575932 2202 90-91 0.2595617986566481 2202 92-93 0.3224935438284433 2202 94-95 0.3099017123939021 2202 96-97 0.3285863429494782 2202 98-99 0.31495786179873164 2202 100-101 0.4088043027301076 1102 1 0.02383111356603962 1102 2 0.052051175835494234 1102 3 0.026314681269560936 1102 4 0.046861124832005885 1102 5 0.06993746655879818 1102 6 0.028624022041036312 1102 7 0.07337561192120035 1102 8 0.06366199229051972 1102 9 0.07742700905570388 1102 10-11 0.04823157878687567 1102 12-13 0.0401348470694316 1102 14-15 0.11017212619594119 1102 16-17 0.09451415912086247 1102 18-19 0.08119338834179501 1102 20-21 0.01569759448045005 1102 22-23 0.07168885732873065 1102 24-25 0.013263801403844866 1102 26-27 0.017856440689875797 1102 28-29 0.0418272162737523 1102 30-31 0.006968813181245537 1102 32-33 0.03938574097169578 1102 34-35 -0.02105554070793403 1102 36-37 -0.041073444932841596 1102 38-39 0.13572502944818865 1102 40-41 0.07494348023775643 1102 42-43 0.08988480685102473 1102 44-45 -0.01506794500004105 1102 46-47 0.03248246445028968 1102 48-49 -0.06205288213327975 1102 50-51 0.019359757071924832 1102 52-53 0.033997302516233674 1102 54-55 -0.05652386997923742 1102 56-57 -0.03657439167078991 1102 58-59 0.05443359312936735 1102 60-61 -0.061587511749948476 1102 62-63 0.021262807741585732 1102 64-65 -4.4724925862027476E-4 1102 66-67 0.10058007398018987 1102 68-69 -0.022484840959883456 1102 70-71 -0.14178325161632443 1102 72-73 -0.1385524950793453 1102 74-75 -0.15565432707091986 1102 76-77 -0.11319749993011641 1102 78-79 0.009563410890443436 1102 80-81 0.0718777577274281 1102 82-83 0.23891419086476873 1102 84-85 0.10322265547925724 1102 86-87 0.13140699222604013 1102 88-89 0.368008315998253 1102 90-91 0.07039347252228367 1102 92-93 0.16998996893994445 1102 94-95 0.19960873909818133 1102 96-97 0.08974583662659086 1102 98-99 0.0115897188437728 1102 100-101 0.16876060162060824 2305 1 0.07360428316019352 2305 2 0.04913453255511513 2305 3 0.05587642549466665 2305 4 0.025714000889976774 2305 5 0.032888053913218585 2305 6 -0.014570225301689277 2305 7 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-1.3244808947845996 1213 84-85 -1.3008707027220652 1213 86-87 -1.0672677076135386 1213 88-89 -0.7497444274236074 1213 90-91 -0.7053787647044807 1213 92-93 -1.014046922083054 1213 94-95 -0.8033313880012329 1213 96-97 -0.8993127955012312 1213 98-99 -0.9823192551663951 1213 100-101 -0.7761962323717633 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 10.0 9 44.0 10 40.0 11 45.0 12 69.0 13 154.0 14 521.0 15 1142.0 16 2263.0 17 3805.0 18 5914.0 19 8038.0 20 10984.0 21 14620.0 22 18965.0 23 24325.0 24 30563.0 25 39737.0 26 52213.0 27 72144.0 28 112401.0 29 156274.0 30 132372.0 31 125373.0 32 164109.0 33 230857.0 34 352225.0 35 607103.0 36 1209851.0 37 2085376.0 38 671497.0 39 604.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.0814902830984 25.31811294736598 22.108208863820973 15.49218790571465 2 28.934198594700238 25.723983058667628 31.61070151189229 13.73111683473984 3 35.937960831869134 19.446625958713703 28.108704027270075 16.506709182147087 4 28.067570899479872 19.87212393825265 32.03403135897138 20.026273803296096 5 34.63381764623214 18.470457500100267 30.269376836389757 16.626348017277838 6 28.866294359073684 19.530399413855204 34.79364122890852 16.809664998162592 7 31.061484228446478 23.106580466600736 27.568744683008685 18.263190621944105 8 27.760360164717905 29.184490509547516 25.40956932900181 17.64557999673277 9 31.981933071368086 23.605240478815347 25.828015934425867 18.584810515390704 10-11 24.453848919191895 30.01860243690293 29.24162869959836 16.285919944306816 12-13 22.713608139247864 28.484318768078587 29.150954131952357 19.65111896072119 14-15 21.905637404750657 28.78153226519074 29.719972062257344 19.592858267801265 16-17 23.135326170778956 27.33239521674667 30.357519589745998 19.174759022728377 18-19 20.706137206010528 27.466276946895142 32.36862038483523 19.458965462259105 20-21 22.72565645380441 27.76166444775515 31.865966005819057 17.646713092621376 22-23 20.875960212928767 31.556587358436534 29.716901190418255 17.850551238216443 24-25 20.726753029767977 27.44650075534291 31.730834131391518 20.09591208349759 26-27 20.697447420274884 27.704830314407207 29.51609631999802 22.081625945319892 28-29 22.31666590040038 29.626976681701787 29.447352452166236 18.609004965731593 30-31 19.9875017077956 28.086292343956394 31.341994750912917 20.58421119733509 32-33 19.980507490008375 30.025916103950053 29.764635604513995 20.228940801527575 34-35 22.269630193369743 29.86253834999718 29.778950110847756 18.088881345785325 36-37 20.258635166204677 29.673582255849844 31.83916021665643 18.228622361289048 38-39 20.86880263128076 27.540962545432198 31.138145663771844 20.452089159515204 40-41 22.5520133774073 27.75680764400914 30.715039441894987 18.976139536688578 42-43 20.441029398481703 29.804126424199385 31.108882347704697 18.645961829614215 44-45 20.172943199760596 27.81952840060881 31.461972133867455 20.54555626576314 46-47 22.2804194044931 27.751708157498584 29.347724894156762 20.620147543851555 48-49 20.407636684219955 30.00945846111604 30.74021059057418 18.842694264089822 50-51 22.675717791746134 27.92913937545117 30.275475345072785 19.119667487729906 52-53 20.615609658354135 27.792602937672346 28.4084473711262 23.18334003284732 54-55 22.60116497135428 28.003614253228122 30.16453526656585 19.23068550885175 56-57 20.5383042220903 28.237803824572154 30.250433576475338 20.973458376862204 58-59 20.539976434065245 28.232245866523463 30.533483612168084 20.694294087243208 60-61 22.20506430847851 28.49125792926202 30.43683816491071 18.866839597348758 62-63 20.168871379513803 28.328121334230467 30.41399158047888 21.08901570577685 64-65 22.08675293787115 28.066470402299014 30.360503493192127 19.48627316663771 66-67 20.344244611473886 31.295121842225353 28.63969133585606 19.7209422104447 68-69 20.235592322906644 31.67614722718269 28.56723653306803 19.52102391684263 70-71 19.94766861016091 32.35653291412986 28.344968012955796 19.350830462753436 72-73 19.954766255489602 32.15536851859569 28.161365545034737 19.728499680879978 74-75 20.21222456621138 31.437077726221972 28.408069494571663 19.942628212994983 76-77 20.136671183446275 31.13221333726513 28.527026956497348 20.204088522791253 78-79 19.911513335575464 31.122202178942327 28.7371959111709 20.22908857431131 80-81 19.81879175425193 31.18251946673589 28.48520182392038 20.513486955091803 82-83 19.543729279513812 30.946983842290305 28.60423047021447 20.905056407981416 84-85 19.689912431955612 30.676795568849872 28.857416787530298 20.775875211664214 86-87 19.99729137735011 30.548110421788483 29.143059305301072 20.31153889556034 88-89 20.217216570647974 30.698424884705005 28.688930261713647 20.395428282933374 90-91 20.29394679867737 30.451092199839923 28.638274319686737 20.616686681795972 92-93 19.85697598658996 30.937728273947414 28.71561787093097 20.489677868531658 94-95 19.58259708130261 31.10285729662116 28.733170848252037 20.58137477382419 96-97 19.765995712818878 31.20775280473756 28.628994117194257 20.397257365249306 98-99 20.27604248852507 30.701127533668416 28.217348597626685 20.805481380179824 100-101 20.47923950715767 30.63447755293292 27.64687834969779 21.239404590211624 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 171.0 1 99.0 2 45.5 3 88.0 4 97.5 5 71.5 6 62.0 7 62.0 8 57.0 9 60.5 10 64.5 11 71.5 12 86.5 13 97.0 14 121.5 15 143.0 16 176.5 17 254.0 18 368.0 19 543.0 20 853.5 21 1356.0 22 2193.5 23 3605.5 24 5546.0 25 8693.0 26 13728.0 27 20514.0 28 28699.0 29 38167.0 30 49245.5 31 61377.0 32 79238.0 33 108889.0 34 147371.5 35 196418.0 36 266531.5 37 340035.0 38 401884.0 39 433203.5 40 439972.0 41 428802.5 42 397342.5 43 369771.0 44 335285.5 45 301493.0 46 281568.0 47 252960.0 48 216119.0 49 186836.0 50 156899.0 51 128227.0 52 104102.0 53 81797.5 54 65154.0 55 51553.5 56 37659.5 57 25832.0 58 17993.0 59 13081.0 60 9565.5 61 6793.5 62 4644.5 63 3066.0 64 2052.5 65 1457.0 66 1017.5 67 711.0 68 505.0 69 346.5 70 239.0 71 159.0 72 107.5 73 83.0 74 63.0 75 45.0 76 38.0 77 30.0 78 18.5 79 15.0 80 11.0 81 6.5 82 3.5 83 3.0 84 2.0 85 0.5 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16603523064126052 2 0.0 3 4.728026010012329E-4 4 0.02109677812743432 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.380167528634719E-4 12-13 0.0 14-15 0.0 16-17 0.0011738547335203022 18-19 0.0 20-21 0.0 22-23 6.113826737084908E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.129981260713462E-4 38-39 7.662662843813085E-4 40-41 0.005086703845254643 42-43 0.003130279289387473 44-45 0.0010352746608130443 46-47 0.004483472940528933 48-49 0.008233286672607676 50-51 0.005388319297617499 52-53 0.0017689338692632333 54-55 0.011371717730977927 56-57 0.019784343321206762 58-59 0.02415369149597678 60-61 0.01202385924960032 62-63 5.706238287945914E-4 64-65 0.015056317311194434 66-67 0.03560692691678251 68-69 0.020623975526433087 70-71 0.03794648461484033 72-73 0.03150658711844422 74-75 0.030626196068304003 76-77 0.03955238310444797 78-79 0.017534455081959516 80-81 0.013116196293292822 82-83 0.001051578198778604 84-85 0.011298351810132909 86-87 0.02233584701281686 88-89 0.021602187804366676 90-91 0.026248696124551205 92-93 0.04417443611768415 94-95 0.02923224357224864 96-97 0.03048761599559674 98-99 0.038076912918564805 100-101 0.01774640107551179 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6133638.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.75749218756526 #Duplication Level Percentage of deduplicated Percentage of total 1 91.49437712269767 50.10002640502267 2 4.337835921508683 4.750580331659032 3 1.3468393658583633 2.21248638161684 4 0.5028372747092562 1.101364325660348 5 0.3776594093519621 1.0339841078575287 6 0.23188123197801072 0.7618340849087357 7 0.1840554772598339 0.7054891450693764 8 0.14296863587298053 0.6262883169505273 9 0.14798525131566947 0.7292971118513386 >10 0.9883612350335867 11.24407649749352 >50 0.13322068868238962 5.066372597084163 >100 0.09459224840925456 9.8259986300351 >500 0.013806701917210177 5.2655752959741795 >1k 0.003460006366045693 2.5996629942287326 >5k 0.0 0.0 >10k+ 1.194290391190238E-4 3.9769637745878152 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 210550 3.432709918648606 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 12164 0.19831623581306884 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 10277 0.1675514596720576 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 10262 0.16730690660257422 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.662662843813085E-4 0.0 0.0 2 7.98873360312428E-4 0.0 0.0 3 9.129981260713462E-4 0.0 0.0 4 9.619087399680255E-4 0.0 0.0 5 0.0011575511955547425 0.0 0.0 6 0.0012227653474169815 0.0 0.0 7 0.001467318416900378 0.0 0.0 8 0.0016792644104526547 0.0 0.0 9 0.0018749068660393717 0.0 0.0 10-11 0.0032770111310775106 0.0 0.0 12-13 0.004353044636804454 0.0 0.0 14-15 0.005722541825911474 0.0 0.0 16-17 0.0071246460909496125 0.0 8.151768982779877E-6 18-19 0.008812062270385047 0.0 5.706238287945914E-5 20-21 0.011110861123528973 0.0 6.521415186223901E-5 22-23 0.013760186042932433 0.0 8.151768982779878E-5 24-25 0.016376903886404774 0.0 8.966945881057865E-5 26-27 0.01927893364427441 0.0 9.782122779335853E-5 28-29 0.0226456142341625 0.0 1.1412476575891828E-4 30-31 0.031710381343013724 0.0 1.2227653474169815E-4 32-33 0.03595745298304204 0.0 1.3042830372447803E-4 34-35 0.0400251857054492 0.0 1.385800727072579E-4 36-37 0.04427225734547752 0.0 1.4673184169003778E-4 38-39 0.04879648913092034 0.0 1.4673184169003778E-4 40-41 0.05363863990669159 0.0 1.4673184169003778E-4 42-43 0.058684584907032336 0.0 1.4673184169003778E-4 44-45 0.06441527850192659 0.0 1.4673184169003778E-4 46-47 0.07035791809037312 0.0 1.4673184169003778E-4 48-49 0.07640653067559579 0.0 1.4673184169003778E-4 50-51 0.08304207062757861 0.0 1.4673184169003778E-4 52-53 0.09042757332597717 0.0 1.4673184169003778E-4 54-55 0.09769895125861683 0.0 1.4673184169003778E-4 56-57 0.105247489336671 0.0 1.4673184169003778E-4 58-59 0.11361935608198592 0.0 1.4673184169003778E-4 60-61 0.1224232665833882 0.0 1.4673184169003778E-4 62-63 0.13147173015427385 0.0 1.6303537965559756E-4 64-65 0.141465798927162 0.0 1.6303537965559756E-4 66-67 0.15157399246580905 0.0 1.9564245558671706E-4 68-69 0.163019076117632 0.0 2.119459935522768E-4 70-71 0.17696675284716834 0.0 2.2824953151783656E-4 72-73 0.19133016979482648 0.0 2.6085660744895606E-4 74-75 0.20674516494126324 0.0 2.9346368338007556E-4 76-77 0.22502958276963853 0.0 2.9346368338007556E-4 78-79 0.24617527151096952 0.0 3.260707593111951E-4 80-81 0.26878012690021813 0.0 3.260707593111951E-4 82-83 0.2942462532024225 0.0 3.260707593111951E-4 84-85 0.32350295208161944 0.0 3.260707593111951E-4 86-87 0.3577892924231916 0.0 3.260707593111951E-4 88-89 0.39730091668272566 0.0 3.4237429727675486E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 27700 0.0 82.83682 3 CGGAAGA 27830 0.0 82.29559 4 GATCGGA 28045 0.0 82.01861 1 ATCGGAA 28500 0.0 80.56158 2 GAAGAGC 29870 0.0 76.60155 6 GGAAGAG 33360 0.0 68.82978 5 AGAGCAC 34350 0.0 66.58336 8 GAGCACA 34600 0.0 66.03363 9 AAGAGCA 36325 0.0 62.98935 7 GCCGTCT 24830 0.0 45.81814 50-51 ATGCCGT 24835 0.0 45.602875 48-49 TATGCCG 24750 0.0 45.51961 48-49 TGCCGTC 24870 0.0 45.448395 50-51 TCGTATG 25045 0.0 45.333733 44-45 CGTATGC 24845 0.0 45.268166 46-47 CGTATCT 24740 0.0 45.210167 38-39 TCGTATC 24625 0.0 45.12235 38-39 TCTCGTA 24940 0.0 45.096287 42-43 TTGTGTT 63210 0.0 45.09048 1 CTCGTAT 24865 0.0 45.02201 44-45 >>END_MODULE