FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_35011-1.341000000358a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_35011-1.341000000358a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6133638
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2105503.432709918648606TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC121640.19831623581306884No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG102770.1675514596720576No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA102620.16730690660257422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG277000.082.836823
CGGAAGA278300.082.295594
GATCGGA280450.082.018611
ATCGGAA285000.080.561582
GAAGAGC298700.076.601556
GGAAGAG333600.068.829785
AGAGCAC343500.066.583368
GAGCACA346000.066.033639
AAGAGCA363250.062.989357
GCCGTCT248300.045.8181450-51
ATGCCGT248350.045.60287548-49
TATGCCG247500.045.5196148-49
TGCCGTC248700.045.44839550-51
TCGTATG250450.045.33373344-45
CGTATGC248450.045.26816646-47
CGTATCT247400.045.21016738-39
TCGTATC246250.045.1223538-39
TCTCGTA249400.045.09628742-43
TTGTGTT632100.045.090481
CTCGTAT248650.045.0220144-45
Produced by FastQC (version 0.11.2)