##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_35010-1.341000000357eb.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6960873 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68447578342544 33.0 33.0 33.0 33.0 33.0 2 32.727975786945116 33.0 33.0 33.0 33.0 33.0 3 32.759805846191995 33.0 33.0 33.0 33.0 33.0 4 36.688762458387046 37.0 37.0 37.0 37.0 37.0 5 36.690872538545094 37.0 37.0 37.0 37.0 37.0 6 36.59259463575905 37.0 37.0 37.0 37.0 37.0 7 36.54765616324274 37.0 37.0 37.0 37.0 37.0 8 36.500731732930625 37.0 37.0 37.0 37.0 37.0 9 36.511141777762646 37.0 37.0 37.0 37.0 37.0 10-11 36.46895030264164 37.0 37.0 37.0 37.0 37.0 12-13 36.448183582720155 37.0 37.0 37.0 37.0 37.0 14-15 38.3899634428038 40.0 37.0 40.0 37.0 40.0 16-17 38.380939790167126 40.0 37.0 40.0 37.0 40.0 18-19 38.39114174328421 40.0 37.0 40.0 37.0 40.0 20-21 38.341106999078995 40.0 37.0 40.0 37.0 40.0 22-23 38.238335191577264 40.0 37.0 40.0 37.0 40.0 24-25 38.22438392425778 40.0 37.0 40.0 37.0 40.0 26-27 38.154203790242974 40.0 37.0 40.0 37.0 40.0 28-29 38.045057854093876 40.0 37.0 40.0 37.0 40.0 30-31 37.99090810879612 40.0 37.0 40.0 35.0 40.0 32-33 37.884097224586625 40.0 37.0 40.0 33.0 40.0 34-35 37.80252124984898 40.0 37.0 40.0 33.0 40.0 36-37 37.70516226915791 40.0 37.0 40.0 33.0 40.0 38-39 37.641172737959735 40.0 37.0 40.0 33.0 40.0 40-41 37.58785176801818 40.0 37.0 40.0 33.0 40.0 42-43 37.44626615081183 40.0 37.0 40.0 33.0 40.0 44-45 37.375806525991784 40.0 37.0 40.0 33.0 40.0 46-47 37.227848935040186 40.0 37.0 40.0 33.0 40.0 48-49 37.151018844906375 40.0 37.0 40.0 33.0 40.0 50-51 37.037175437621116 38.5 37.0 40.0 33.0 40.0 52-53 36.910291280993064 37.0 37.0 40.0 33.0 40.0 54-55 36.777389991169215 37.0 37.0 40.0 33.0 40.0 56-57 36.587949097764024 37.0 37.0 40.0 33.0 40.0 58-59 36.361517370019534 37.0 37.0 40.0 33.0 40.0 60-61 36.15524388966729 37.0 37.0 40.0 33.0 40.0 62-63 35.92787434851921 37.0 37.0 40.0 33.0 40.0 64-65 35.697956635611654 37.0 35.0 40.0 30.0 40.0 66-67 35.425602435211786 37.0 33.0 40.0 27.0 40.0 68-69 35.142191360192896 37.0 33.0 37.0 27.0 40.0 70-71 34.83631859107327 37.0 33.0 37.0 27.0 40.0 72-73 34.51458452122313 37.0 33.0 37.0 27.0 40.0 74-75 34.11915409173533 37.0 33.0 37.0 27.0 38.5 76-77 31.36335219734651 33.0 30.0 35.0 24.5 37.0 78-79 33.74624016269223 37.0 33.0 37.0 27.0 37.0 80-81 34.0642209676861 37.0 33.0 37.0 27.0 37.0 82-83 33.920170501602314 37.0 33.0 37.0 27.0 37.0 84-85 33.773899480711684 37.0 33.0 37.0 27.0 37.0 86-87 33.668066778405525 37.0 33.0 37.0 27.0 37.0 88-89 33.398945864979865 37.0 33.0 37.0 27.0 37.0 90-91 33.36894330639275 37.0 33.0 37.0 27.0 37.0 92-93 33.21746058288953 37.0 33.0 37.0 27.0 37.0 94-95 33.04773919880452 37.0 33.0 37.0 27.0 37.0 96-97 32.94829276442768 35.0 33.0 37.0 27.0 37.0 98-99 32.83511349797647 33.0 33.0 37.0 27.0 37.0 100-101 31.212537349841035 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.1549122605322495 2306 2 0.1496124057198429 2306 3 0.1380332474150876 2306 4 0.15423658030154286 2306 5 0.13953448802436696 2306 6 0.16965181946003582 2306 7 0.1597427463067831 2306 8 0.2384285333495768 2306 9 0.16596349869012528 2306 10-11 0.19908653008364752 2306 12-13 0.21500043666051027 2306 14-15 0.43481357991119296 2306 16-17 0.41787530874775314 2306 18-19 0.4083136768821518 2306 20-21 0.40758769634787484 2306 22-23 0.3881973935178422 2306 24-25 0.4392727036205528 2306 26-27 0.449979126585049 2306 28-29 0.4414950877468371 2306 30-31 0.48709032621020754 2306 32-33 0.5449605382210407 2306 34-35 0.502322761836318 2306 36-37 0.4962937704489079 2306 38-39 0.5057338908500171 2306 40-41 0.5257226081258395 2306 42-43 0.5320356250337852 2306 44-45 0.4496975008009656 2306 46-47 0.6245960063638805 2306 48-49 0.5645891681161785 2306 50-51 0.646022379884414 2306 52-53 0.6097651813264022 2306 54-55 0.5610422614325259 2306 56-57 0.49678476927008575 2306 58-59 0.5388388185600448 2306 60-61 0.6206194248976402 2306 62-63 0.565760506758096 2306 64-65 0.48205891856800775 2306 66-67 0.5084030839964271 2306 68-69 0.4660047903842397 2306 70-71 0.4080067090268713 2306 72-73 0.38643907322454396 2306 74-75 0.4699311993002553 2306 76-77 -0.007798409127175887 2306 78-79 0.3798824975331243 2306 80-81 0.4811615762049968 2306 82-83 0.5212055846048926 2306 84-85 0.5465290490110561 2306 86-87 0.4802749381605267 2306 88-89 0.7342798215560578 2306 90-91 0.5352011614833216 2306 92-93 0.41626075383219785 2306 94-95 0.3467821795986552 2306 96-97 0.376909409796518 2306 98-99 0.39416710874126437 2306 100-101 0.37517511808383475 2307 1 0.1771648697840149 2307 2 0.11546643871453455 2307 3 0.09963046685763999 2307 4 0.16794531665667733 2307 5 0.1360780836153168 2307 6 0.14221825472611016 2307 7 0.1739440698734782 2307 8 0.21710254519113903 2307 9 0.2276466143799638 2307 10-11 0.20217624370151555 2307 12-13 0.2299641930959737 2307 14-15 0.3665335684222981 2307 16-17 0.31973393297259634 2307 18-19 0.3484189619895375 2307 20-21 0.3664639439079238 2307 22-23 0.35872595654430484 2307 24-25 0.3679773308751919 2307 26-27 0.41880312960981314 2307 28-29 0.4072897109735223 2307 30-31 0.36142072673842307 2307 32-33 0.41772798404816314 2307 34-35 0.39189476539333157 2307 36-37 0.3590522029397576 2307 38-39 0.451847202857131 2307 40-41 0.3649487098029027 2307 42-43 0.3823543823292894 2307 44-45 0.43094991009694184 2307 46-47 0.56009365377313 2307 48-49 0.3876556325912972 2307 50-51 0.4090521410228902 2307 52-53 0.3369950580029055 2307 54-55 0.3198822816552891 2307 56-57 0.3266245783582846 2307 58-59 0.466287005849928 2307 60-61 0.4116388721758426 2307 62-63 0.390565743688569 2307 64-65 0.34026517900529285 2307 66-67 0.34455207071280114 2307 68-69 0.3690890002595708 2307 70-71 0.22036916053894373 2307 72-73 0.2794612607444904 2307 74-75 0.29362036198493513 2307 76-77 -0.09504250989047236 2307 78-79 0.15678475503219147 2307 80-81 0.3270312721027082 2307 82-83 0.3958515150087365 2307 84-85 0.3267799800044955 2307 86-87 0.3888674657639797 2307 88-89 0.4942177054432264 2307 90-91 0.2988623204407759 2307 92-93 0.3278845318751351 2307 94-95 0.3502273376214262 2307 96-97 0.3145603006906228 2307 98-99 0.2887168440026855 2307 100-101 0.25687571052272773 2201 1 0.17873551061487092 2201 2 0.1692635203453463 2201 3 0.12553912451872407 2201 4 0.1574858410809199 2201 5 0.15107220243657338 2201 6 0.17340417421087295 2201 7 0.15832750673814644 2201 8 0.1933719984794564 2201 9 0.18087344565110897 2201 10-11 0.1885642558174183 2201 12-13 0.19356084511965577 2201 14-15 0.3650059466785933 2201 16-17 0.34125381889460016 2201 18-19 0.3122787410846186 2201 20-21 0.3611487389955741 2201 22-23 0.40236095266053695 2201 24-25 0.36787466880586095 2201 26-27 0.35693260894053935 2201 28-29 0.37369377085862965 2201 30-31 0.30497378354912286 2201 32-33 0.474900858470626 2201 34-35 0.419969097807531 2201 36-37 0.35418761979281044 2201 38-39 0.4346273221507104 2201 40-41 0.35493477999406053 2201 42-43 0.42103507702536547 2201 44-45 0.4669483202942146 2201 46-47 0.514113038757543 2201 48-49 0.43313162990761356 2201 50-51 0.4544367069165176 2201 52-53 0.47477758014726845 2201 54-55 0.4752360705011043 2201 56-57 0.4219400281875636 2201 58-59 0.486436816331171 2201 60-61 0.4211377784885144 2201 62-63 0.5267499608103492 2201 64-65 0.4554473889503683 2201 66-67 0.3687082398973516 2201 68-69 0.4301087835829591 2201 70-71 0.3362583936228276 2201 72-73 0.42884237597435515 2201 74-75 0.3749549891902788 2201 76-77 0.10910139568174415 2201 78-79 0.4176044678753428 2201 80-81 0.48640284064842376 2201 82-83 0.5490980665339933 2201 84-85 0.5243477391910076 2201 86-87 0.49116482460775757 2201 88-89 0.7439926800854408 2201 90-91 0.4681366544285339 2201 92-93 0.49263868921307363 2201 94-95 0.600079721240526 2201 96-97 0.5513279023181354 2201 98-99 0.6225203416955765 2201 100-101 0.46620094154728164 1101 1 0.11754549450618867 1101 2 0.11922639943330182 1101 3 0.12089247256535174 1101 4 0.11564822630147376 1101 5 0.10859212404272967 1101 6 0.09044971356119902 1101 7 0.10031866668194311 1101 8 0.09318223101916345 1101 9 0.08317755362492107 1101 10-11 0.10159862801475583 1101 12-13 0.1197472613234396 1101 14-15 0.22776721274502165 1101 16-17 0.21917796541472967 1101 18-19 0.22025244131146593 1101 20-21 0.17767588753883956 1101 22-23 0.1890811926056699 1101 24-25 0.24691643862108492 1101 26-27 0.24933816973458534 1101 28-29 0.22141593661874026 1101 30-31 0.24926718294556593 1101 32-33 0.269037908044929 1101 34-35 0.2510573729384973 1101 36-37 0.2703787609538466 1101 38-39 0.3889155275835847 1101 40-41 0.3690060013424201 1101 42-43 0.3206702388867626 1101 44-45 0.24996586792171627 1101 46-47 0.3484465749642496 1101 48-49 0.3122044667013384 1101 50-51 0.2770735444812047 1101 52-53 0.15699505177322948 1101 54-55 0.2538228216429417 1101 56-57 0.312118015334768 1101 58-59 0.3105534813900874 1101 60-61 0.28234365076279744 1101 62-63 0.3872747608701417 1101 64-65 0.28826170113215 1101 66-67 0.2504932089052261 1101 68-69 0.18097902480565864 1101 70-71 0.11482043751183824 1101 72-73 0.11399003023174714 1101 74-75 0.08924183713126155 1101 76-77 -0.17143490216173163 1101 78-79 0.11901682974876593 1101 80-81 0.2534259596207349 1101 82-83 0.35777963987234074 1101 84-85 0.3558186671965444 1101 86-87 0.2774494327246515 1101 88-89 0.5270269243378465 1101 90-91 0.34101763229394777 1101 92-93 0.32032565548622216 1101 94-95 0.5241845373238263 1101 96-97 0.40023792975950556 1101 98-99 0.3219571176365861 1101 100-101 0.26578671891943983 2304 1 0.07524490213729251 2304 2 0.08454870711457119 2304 3 0.08316992850534177 2304 4 0.079659942617063 2304 5 0.09041377898736869 2304 6 0.10150316968026374 2304 7 0.1014552748492008 2304 8 0.10434708691841621 2304 9 0.06891530441897942 2304 10-11 0.08430487301387046 2304 12-13 0.12776329991162072 2304 14-15 0.27127285984573746 2304 16-17 0.253356642434305 2304 18-19 0.2553521020037408 2304 20-21 0.2388919779429841 2304 22-23 0.23315671485408984 2304 24-25 0.2722399344802966 2304 26-27 0.18764410333260173 2304 28-29 0.21349284995531548 2304 30-31 0.3276257041071631 2304 32-33 0.15432363617173905 2304 34-35 0.26385483974831203 2304 36-37 0.2593186156650802 2304 38-39 0.28931197669372466 2304 40-41 0.29153109844309455 2304 42-43 0.30355633023063433 2304 44-45 0.3066392184672182 2304 46-47 0.3824037319498643 2304 48-49 0.292493508153683 2304 50-51 0.3013548579827656 2304 52-53 0.27166969072083447 2304 54-55 0.4003797278348955 2304 56-57 0.2615633924657814 2304 58-59 0.38441023993117795 2304 60-61 0.29817495566884844 2304 62-63 0.2684729851984571 2304 64-65 0.1887473283739709 2304 66-67 0.2636970630201674 2304 68-69 0.2511243955260909 2304 70-71 0.2177868915363348 2304 72-73 0.09172552569044967 2304 74-75 0.18572211264861238 2304 76-77 0.10708236449646336 2304 78-79 0.24314793008931446 2304 80-81 0.2841770445376497 2304 82-83 0.3845090437163705 2304 84-85 0.37458196637538776 2304 86-87 0.27686969388603444 2304 88-89 0.5969025903273035 2304 90-91 0.3846551354008483 2304 92-93 0.42915925638094876 2304 94-95 0.3891154297785917 2304 96-97 0.3695313205326727 2304 98-99 0.38885134132637234 2304 100-101 0.4337446760787529 2202 1 0.08816218469059578 2202 2 0.08958979877026962 2202 3 0.07211115128011159 2202 4 0.07809687212139949 2202 5 0.03486072986549971 2202 6 0.009864614168733965 2202 7 0.06385652477380432 2202 8 0.09369750225406648 2202 9 0.12283285497924368 2202 10-11 0.10832265263006491 2202 12-13 0.0806176001054908 2202 14-15 0.06255374325542107 2202 16-17 0.0994007448836598 2202 18-19 0.09342056224866013 2202 20-21 0.18436923684531337 2202 22-23 0.16086966109919842 2202 24-25 0.13597446197690743 2202 26-27 0.09442867668634847 2202 28-29 0.11410167345675859 2202 30-31 0.11449945820208285 2202 32-33 0.1749056925027972 2202 34-35 0.15171129472049216 2202 36-37 0.18207896775565047 2202 38-39 0.18884686481042223 2202 40-41 0.23924674874640317 2202 42-43 0.25923083075660003 2202 44-45 0.15386536371578075 2202 46-47 0.3510124081674135 2202 48-49 0.1899246586440313 2202 50-51 0.20960715458974022 2202 52-53 0.09636749935565803 2202 54-55 0.14531650457845302 2202 56-57 0.26871319286806994 2202 58-59 0.3300657510668188 2202 60-61 0.17663407139846754 2202 62-63 0.18454748402500343 2202 64-65 0.22175602925059934 2202 66-67 0.15532239303968964 2202 68-69 0.10982260017421908 2202 70-71 0.01937725695037784 2202 72-73 5.6694339961183E-4 2202 74-75 -0.01520458509083511 2202 76-77 -0.2522175191128504 2202 78-79 0.10785217285663862 2202 80-81 0.19895334398197662 2202 82-83 0.31453457244197836 2202 84-85 0.3362825629127002 2202 86-87 0.3242973313550621 2202 88-89 0.49327940089527544 2202 90-91 0.23459809902146844 2202 92-93 0.341730950700871 2202 94-95 0.3835769948831995 2202 96-97 0.3339067265857736 2202 98-99 0.28743599402729814 2202 100-101 0.2544881753202404 1102 1 0.07730976286865854 1102 2 0.06857154711989466 1102 3 0.07551908381212513 1102 4 0.06044613794764331 1102 5 0.057802305134558196 1102 6 0.010182057932972555 1102 7 0.06711723103256872 1102 8 0.05482118307173067 1102 9 0.0383379646871731 1102 10-11 0.03561537513897406 1102 12-13 0.06785078567042291 1102 14-15 0.13066635991040698 1102 16-17 0.18110505034745472 1102 18-19 0.09512071382831522 1102 20-21 0.14909386559192228 1102 22-23 0.11473755072681513 1102 24-25 0.09888386706806784 1102 26-27 0.1486784396151961 1102 28-29 0.09734692662731703 1102 30-31 0.13111895120295003 1102 32-33 0.1897837296342928 1102 34-35 0.10512394792686308 1102 36-37 0.14608218260835315 1102 38-39 0.20248927863775634 1102 40-41 0.2001609568885243 1102 42-43 0.19844321091906636 1102 44-45 0.11370394629379632 1102 46-47 0.22491938131870626 1102 48-49 0.08646795483151237 1102 50-51 0.15469612351430584 1102 52-53 0.14961500499540392 1102 54-55 0.16734911483712978 1102 56-57 0.12575209980784763 1102 58-59 0.20358024969730337 1102 60-61 0.11239177623365038 1102 62-63 0.130262022848882 1102 64-65 0.12538861072385998 1102 66-67 0.16470815817157103 1102 68-69 0.09202234576041235 1102 70-71 -0.04240874105263259 1102 72-73 -0.07324393831797238 1102 74-75 -0.043753182248948974 1102 76-77 -0.01676806670978337 1102 78-79 0.16718638430671007 1102 80-81 0.15681506845785265 1102 82-83 0.2850859155041192 1102 84-85 0.2374300695396272 1102 86-87 0.2307131904702473 1102 88-89 0.3584894691000642 1102 90-91 0.1631559699914078 1102 92-93 0.2156381171639623 1102 94-95 0.20996212618431542 1102 96-97 0.14600967218604666 1102 98-99 0.10847061532160751 1102 100-101 0.13925982626210853 2305 1 0.05813843658048512 2305 2 0.05406682525767792 2305 3 0.046738397686503674 2305 4 0.052259016623331433 2305 5 0.023501887631248053 2305 6 0.011116749340168042 2305 7 0.04671022738919106 2305 8 0.0034661139339604574 2305 9 0.04055561185962375 2305 10-11 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-0.8540207231712387 1213 92-93 -1.0370712436443483 1213 94-95 -0.8955629266482887 1213 96-97 -0.8578639655738272 1213 98-99 -1.0067576439128842 1213 100-101 -0.8161175086816392 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 7.0 9 17.0 10 19.0 11 14.0 12 40.0 13 195.0 14 670.0 15 1565.0 16 3285.0 17 4936.0 18 6209.0 19 7855.0 20 10126.0 21 12967.0 22 16618.0 23 21339.0 24 27539.0 25 34778.0 26 44802.0 27 58899.0 28 80787.0 29 108834.0 30 119168.0 31 137400.0 32 184196.0 33 261813.0 34 403079.0 35 693527.0 36 1363560.0 37 2419437.0 38 936753.0 39 439.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.970483534377884 27.705294676830704 20.14130823003376 16.182913558757647 2 30.650106674838057 24.588036586790192 30.184978234770266 14.576878503601487 3 35.73072867433047 20.959680269392 25.424272288194278 17.885318768083255 4 29.677114634320173 21.354510330906272 30.077599624790498 18.890775409983057 5 30.550076118325965 19.748657962873335 31.43262346547624 18.26864245332446 6 27.51055794294767 20.122849533384677 33.73525131115019 18.63134121251745 7 31.985772474228447 21.201737195894825 26.317632860131194 20.494857469745533 8 29.187746996677 27.665639065674664 23.533729174487167 19.612884763161173 9 27.954209191864297 24.86484094739266 26.657302898645042 20.523646962098002 10-11 22.761719705963205 29.281064850101558 30.870537771396357 17.086677672538883 12-13 22.156315737982865 28.176408332690457 30.767663768610632 18.899612160716046 14-15 21.666678877778693 28.3678426542188 31.32260134612426 18.64287712187825 16-17 21.873665740143213 27.797786687369303 31.967005747050475 18.361541825437005 18-19 20.940620522741902 27.810060605903885 32.49893368259987 18.750385188754343 20-21 21.60829539628147 28.19946578539789 31.91995458040967 18.272284237910963 22-23 21.077919264411253 29.685306929646195 30.74068152060827 18.49609228533428 24-25 21.06089279318844 28.154679736291698 31.403762143053033 19.38066532746683 26-27 21.111884960406545 28.28833394891704 30.693341194416274 19.90643989626014 28-29 21.662792870951677 29.079010635591253 30.43873950867944 18.819456984777627 30-31 20.883508433496775 28.70308077736801 30.967301084217453 19.44610970491776 32-33 20.781840151371817 29.370195376355813 30.36435228742142 19.48361218485095 34-35 21.523399435674232 29.21782943030278 30.527492744085404 18.731278389937582 36-37 21.398161331766655 29.049071809141452 30.75154878053511 18.801218078556786 38-39 21.094812026829306 28.534200817274343 31.481741268863345 18.889245887033002 40-41 21.006441695205634 28.700709897439303 29.915117600895048 20.37773080646001 42-43 20.807113504767788 29.181483056213604 30.725332709250953 19.28607072976766 44-45 20.691763529885595 28.449057641118586 30.914829013633266 19.94434981536255 46-47 21.398783704446835 28.517792709682976 30.10285879953794 19.980564786332252 48-49 20.84956529822253 29.46006264969745 30.28068231074888 19.409689741331142 50-51 21.567585576540978 28.842988730397582 30.024499697866275 19.564925995195168 52-53 20.801471445983292 28.77804358482076 29.484308637030495 20.936176332165456 54-55 21.387512828503436 28.924272067288815 30.002577553987468 19.685637550220278 56-57 20.692136008082123 28.845564451875312 30.195937758682057 20.26636178136051 58-59 20.759361782733198 28.921069048480007 30.276717105469714 20.042852063317078 60-61 21.153607152365495 28.98106866232369 30.268387555428866 19.596936629881952 62-63 20.330041006592314 28.89714189338286 30.255420616386086 20.517396483638738 64-65 21.034756867876776 28.77184449802959 30.18052478879746 20.012873845296177 66-67 20.541028993668426 29.75109780627004 29.65615686574325 20.051716334318286 68-69 20.466845224068464 30.014038405168204 29.529533815744855 19.98958255501848 70-71 20.299134643643193 30.298128665980208 29.35880420872312 20.04393248165348 72-73 20.29440182008693 30.181144318439912 29.375680838697715 20.148773022775444 74-75 20.415323292201982 30.001355110130362 29.40794476783788 20.175376829829784 76-77 20.474247900528272 29.852620027324445 29.404086968275088 20.269045103872195 78-79 20.30774471934626 29.81037105494679 29.64904219249775 20.232842033209206 80-81 20.221844474527607 30.02195700381295 29.52078357854047 20.235414943118975 82-83 20.14479931312557 29.923723637483672 29.483286168061127 20.448190881329626 84-85 20.241871067660927 29.61029816220315 29.645189629052055 20.502641141083867 86-87 20.31138291913009 29.60906646408231 29.756140808291743 20.323409808495853 88-89 20.502499731839308 29.90086208723639 29.351824171649284 20.244814009275018 90-91 20.608326516160545 29.73382778133106 29.314810549175537 20.34303515333286 92-93 20.304234614996293 29.834789760349757 29.29133247172929 20.56964315292466 94-95 20.1053547928556 29.93062039999644 29.194763529388435 20.76926127775953 96-97 20.10693694821298 30.216980724017283 29.070896167436448 20.605186160333282 98-99 20.397223471112362 30.158159191900495 28.92323458530448 20.52138275168266 100-101 20.4882426613486 30.0123806077425 28.653725113150045 20.845651617758847 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 153.0 1 89.0 2 31.0 3 44.5 4 48.0 5 34.5 6 36.5 7 62.5 8 77.5 9 82.5 10 101.0 11 116.0 12 132.0 13 156.5 14 170.5 15 212.5 16 265.0 17 331.0 18 455.5 19 572.5 20 810.5 21 1277.0 22 2120.5 23 3658.5 24 6042.0 25 9901.5 26 16987.5 27 26890.5 28 38609.0 29 52568.0 30 69198.0 31 88559.0 32 113906.5 33 149305.0 34 192153.0 35 239749.5 36 295188.5 37 356559.0 38 414053.0 39 448313.0 40 466395.5 41 462008.5 42 433862.5 43 411637.0 44 381731.5 45 349833.5 46 331784.0 47 303051.5 48 259925.5 49 220232.0 50 181228.0 51 145160.5 52 116576.0 53 92793.0 54 75344.5 55 59285.5 56 42017.0 57 28615.5 58 20304.0 59 14962.5 60 10866.5 61 7608.0 62 5277.0 63 3457.0 64 2285.5 65 1606.5 66 1130.5 67 785.5 68 559.5 69 406.5 70 303.0 71 217.0 72 160.0 73 125.5 74 94.5 75 71.5 76 59.0 77 46.5 78 36.5 79 27.0 80 14.0 81 8.5 82 8.5 83 6.5 84 4.0 85 2.5 86 3.0 87 3.5 88 2.5 89 0.5 90 1.0 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16760828706399328 2 0.0 3 1.8675818392319468E-4 4 0.0224253480849313 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.249216154353053E-4 12-13 0.0 14-15 0.0 16-17 0.0012211112025747345 18-19 0.0 20-21 0.0 22-23 3.878823819943275E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0010630850469474159 38-39 7.542157427667479E-4 40-41 0.005178948100331668 42-43 0.0036417845865022963 44-45 9.984379832816948E-4 46-47 0.004475013407082703 48-49 0.008389752262395822 50-51 0.0054447193620685225 52-53 0.0014509674289417435 54-55 0.011291687120279309 56-57 0.018905674618686478 58-59 0.02271985137496403 60-61 0.011866327686196831 62-63 5.890065800654602E-4 64-65 0.014732347508710473 66-67 0.033566192056657264 68-69 0.02001185770807771 70-71 0.035455322917111114 72-73 0.029945956491376872 74-75 0.02914864270616631 76-77 0.0378472642727428 78-79 0.016894432637975153 80-81 0.013029974832179814 82-83 9.553399408378805E-4 84-85 0.010321981165293492 86-87 0.021082125762099092 88-89 0.020493119182033632 90-91 0.024881936504228708 92-93 0.041955944319053085 94-95 0.02745345303670962 96-97 0.028818224380763733 98-99 0.03551996998077684 100-101 0.017217667956303754 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6960873.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.09030921273778 #Duplication Level Percentage of deduplicated Percentage of total 1 91.61552528453721 59.632828694579246 2 4.6344872767225525 6.0332041976874 3 1.2675151118284758 2.475088516822002 4 0.593921873822111 1.5463423366115936 5 0.33428015762343194 1.0879199411695955 6 0.21369513405125115 0.8345689411592033 7 0.19921345352147157 0.9076805702334965 8 0.12325368721035443 0.6418096489705627 9 0.10755569655171283 0.6300750191528145 >10 0.7466878412588247 9.898502727039984 >50 0.09781441641926814 4.388323096983024 >100 0.05957211851993849 7.754790591249557 >500 0.005684322767541307 2.4449457261789718 >1k 7.715393981047649E-4 0.6663288989504874 >5k 0.0 0.0 >10k+ 2.2085767723461422E-5 1.057591093212012 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 73568 1.0568789288355067 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.768889620597877E-4 0.0 0.0 2 0.0010630850469474159 0.0 0.0 3 0.0011923791742788584 0.0 0.0 4 0.0012642092450185487 0.0 0.0 5 0.0014509674289417433 0.0 0.0 6 0.0017239216977525664 0.0 1.4366014147938053E-5 7 0.0021118040797468937 0.0 1.4366014147938053E-5 8 0.002413490376853593 0.0 1.4366014147938053E-5 9 0.0027295426881082303 0.0 2.8732028295876105E-5 10-11 0.004661771591005898 0.0 2.8732028295876105E-5 12-13 0.006902869798084235 0.0 4.309804244381416E-5 14-15 0.009574948429600712 0.0 5.0281049517783186E-5 16-17 0.012742654549221053 0.0 5.746405659175221E-5 18-19 0.015802615562731857 0.0 5.746405659175221E-5 20-21 0.01822328894665942 0.0 5.746405659175221E-5 22-23 0.020687060373030797 0.0 6.464706366572123E-5 24-25 0.02322984487721583 0.0 7.183007073969026E-5 26-27 0.02599530260069391 0.0 7.183007073969026E-5 28-29 0.029500610052790793 0.0 7.90130778136593E-5 30-31 0.047709532985302276 0.0 9.337909196159734E-5 32-33 0.05317580136859271 0.0 1.0056209903556637E-4 34-35 0.05733476246442077 0.0 1.1492811318350442E-4 36-37 0.06203963209787049 0.0 1.2929412733144247E-4 38-39 0.0669528089364653 0.0 1.2929412733144247E-4 40-41 0.07243344333390367 0.0 1.364771344054115E-4 42-43 0.07886223466510595 0.0 1.4366014147938053E-4 44-45 0.0862463659371461 0.0 1.4366014147938053E-4 46-47 0.09375979133651771 0.0 1.5084314855334956E-4 48-49 0.102041798492804 0.0 1.580261556273186E-4 50-51 0.1113653416748158 0.0 1.580261556273186E-4 52-53 0.1211270482883397 0.0 1.580261556273186E-4 54-55 0.13171480071537003 0.0 1.6520916270128762E-4 56-57 0.1429202917507617 0.0 1.7957517684922565E-4 58-59 0.1555623842009472 0.0 1.8675818392319468E-4 60-61 0.16867855511801466 0.0 1.9394119099716371E-4 62-63 0.18445962165952462 0.0 2.154902122190708E-4 64-65 0.20095180590135747 0.0 2.2985622636700884E-4 66-67 0.2196635393290468 0.0 2.4422224051494693E-4 68-69 0.2416722730036879 0.0 2.4422224051494693E-4 70-71 0.2651535231284926 0.0 2.5140524758891596E-4 72-73 0.29296612651890075 0.0 2.6577126173685397E-4 74-75 0.32225124635947244 0.0 2.72954268810823E-4 76-77 0.35684460842770727 0.0 2.72954268810823E-4 78-79 0.3956759446695838 0.0 2.72954268810823E-4 80-81 0.4390541243892828 0.0 2.72954268810823E-4 82-83 0.4884732130581897 0.0 2.80137275884792E-4 84-85 0.5422739360422177 0.0 2.945032900327301E-4 86-87 0.605520313328515 0.0 3.160523112546372E-4 88-89 0.6749368936913516 0.0 3.232353183286062E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 15185 0.0 54.682434 3 CGGAAGA 15270 0.0 54.378048 4 GATCGGA 15995 0.0 51.941208 1 ATCGGAA 16120 0.0 51.451782 2 GAAGAGC 17950 0.0 46.244587 6 GCCGTCT 9840 0.0 42.23963 50-51 TATGCCG 9770 0.0 42.04927 48-49 TGCCGTC 9795 0.0 41.97298 50-51 CGTATGC 9885 0.0 41.462498 46-47 ATGCCGT 9985 0.0 41.453026 48-49 CTCGTAT 9935 0.0 40.65659 44-45 TCTCGTA 10040 0.0 40.515507 42-43 TCGTATG 10320 0.0 40.24432 44-45 CCGTCTT 10265 0.0 40.122463 52-53 GTATGCC 10495 0.0 39.686108 46-47 CATCTCG 10135 0.0 39.642563 40-41 ATCTCGT 10285 0.0 38.903904 42-43 CGTCTTC 10725 0.0 38.844517 52-53 CGAGATT 10505 0.0 38.515892 32-33 TCACGAG 10565 0.0 38.00416 30-31 >>END_MODULE