FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_35010-1.341000000357eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_35010-1.341000000357eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6960873
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT735681.0568789288355067TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG151850.054.6824343
CGGAAGA152700.054.3780484
GATCGGA159950.051.9412081
ATCGGAA161200.051.4517822
GAAGAGC179500.046.2445876
GCCGTCT98400.042.2396350-51
TATGCCG97700.042.0492748-49
TGCCGTC97950.041.9729850-51
CGTATGC98850.041.46249846-47
ATGCCGT99850.041.45302648-49
CTCGTAT99350.040.6565944-45
TCTCGTA100400.040.51550742-43
TCGTATG103200.040.2443244-45
CCGTCTT102650.040.12246352-53
GTATGCC104950.039.68610846-47
CATCTCG101350.039.64256340-41
ATCTCGT102850.038.90390442-43
CGTCTTC107250.038.84451752-53
CGAGATT105050.038.51589232-33
TCACGAG105650.038.0041630-31
Produced by FastQC (version 0.11.2)