##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_35008-2.341000000358b3.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5888638 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67170252272257 33.0 33.0 33.0 33.0 33.0 2 32.71382041144319 33.0 33.0 33.0 33.0 33.0 3 32.74846560444028 33.0 33.0 33.0 33.0 33.0 4 36.671134819290984 37.0 37.0 37.0 37.0 37.0 5 36.67567169182416 37.0 37.0 37.0 37.0 37.0 6 36.57293520165444 37.0 37.0 37.0 37.0 37.0 7 36.506886651887925 37.0 37.0 37.0 37.0 37.0 8 36.45614894310025 37.0 37.0 37.0 37.0 37.0 9 36.48873491629134 37.0 37.0 37.0 37.0 37.0 10-11 36.42925673135282 37.0 37.0 37.0 37.0 37.0 12-13 36.399025971710266 37.0 37.0 37.0 37.0 37.0 14-15 38.30177538507206 40.0 37.0 40.0 37.0 40.0 16-17 38.29430795372376 40.0 37.0 40.0 37.0 40.0 18-19 38.271073718574655 40.0 37.0 40.0 37.0 40.0 20-21 38.247063072989036 40.0 37.0 40.0 37.0 40.0 22-23 38.114201959774064 40.0 37.0 40.0 37.0 40.0 24-25 38.10793548525143 40.0 37.0 40.0 37.0 40.0 26-27 38.03849531929115 40.0 37.0 40.0 37.0 40.0 28-29 37.890129347397476 40.0 37.0 40.0 33.0 40.0 30-31 37.84490743020712 40.0 37.0 40.0 33.0 40.0 32-33 37.7199544274924 40.0 37.0 40.0 33.0 40.0 34-35 37.59326647350372 40.0 37.0 40.0 33.0 40.0 36-37 37.402889768398055 40.0 37.0 40.0 33.0 40.0 38-39 37.43401538691969 40.0 37.0 40.0 33.0 40.0 40-41 37.280787255049475 40.0 37.0 40.0 33.0 40.0 42-43 37.12529085673121 40.0 37.0 40.0 33.0 40.0 44-45 37.11866742360458 40.0 37.0 40.0 33.0 40.0 46-47 36.987763469243646 40.0 37.0 40.0 33.0 40.0 48-49 36.86266179038344 37.0 37.0 40.0 33.0 40.0 50-51 36.78165952806065 37.0 37.0 40.0 33.0 40.0 52-53 36.66819008062645 37.0 37.0 40.0 33.0 40.0 54-55 36.47351059786661 37.0 37.0 40.0 33.0 40.0 56-57 36.30300393741303 37.0 37.0 40.0 33.0 40.0 58-59 36.048449488659344 37.0 37.0 40.0 33.0 40.0 60-61 35.85885972274064 37.0 37.0 40.0 27.0 40.0 62-63 35.65561629021855 37.0 35.0 40.0 27.0 40.0 64-65 35.424479395744825 37.0 33.0 40.0 27.0 40.0 66-67 35.05508370526427 37.0 33.0 40.0 27.0 40.0 68-69 34.80543845622706 37.0 33.0 38.5 27.0 40.0 70-71 34.447448034672874 37.0 33.0 37.0 27.0 40.0 72-73 33.95429223192188 37.0 33.0 37.0 27.0 40.0 74-75 33.37659166686762 37.0 33.0 37.0 22.0 40.0 76-77 30.726767463036445 33.0 30.0 35.0 22.0 37.0 78-79 32.96494817647137 37.0 33.0 37.0 22.0 37.0 80-81 33.211014923994306 37.0 33.0 37.0 27.0 37.0 82-83 33.01771411317863 37.0 33.0 37.0 22.0 37.0 84-85 32.83239851048748 37.0 33.0 37.0 22.0 37.0 86-87 32.691809973715486 37.0 33.0 37.0 22.0 37.0 88-89 32.37966945837051 37.0 33.0 37.0 22.0 37.0 90-91 32.32312938917285 37.0 33.0 37.0 22.0 37.0 92-93 32.156499176889454 33.0 33.0 37.0 22.0 37.0 94-95 31.95718093046304 33.0 33.0 37.0 22.0 37.0 96-97 31.84154451334927 33.0 33.0 37.0 22.0 37.0 98-99 31.706638360177685 33.0 33.0 37.0 15.0 37.0 100-101 30.092319565237325 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.1447959020120564 2306 2 0.13615485611310874 2306 3 0.12306134206889396 2306 4 0.1597582177752912 2306 5 0.12437093959060519 2306 6 0.18007436594094628 2306 7 0.1497339956814443 2306 8 0.19719677797481694 2306 9 0.19509288471385844 2306 10-11 0.17643355888323953 2306 12-13 0.20080985400556273 2306 14-15 0.38291744584240206 2306 16-17 0.3747621408382358 2306 18-19 0.421556331641213 2306 20-21 0.3961006131551983 2306 22-23 0.343471530544889 2306 24-25 0.34867418487918656 2306 26-27 0.3665988850075905 2306 28-29 0.3555769261803974 2306 30-31 0.37650147686238 2306 32-33 0.4101768977836997 2306 34-35 0.46239901054757837 2306 36-37 0.28227380296262794 2306 38-39 0.3198745192777963 2306 40-41 0.41923925047796473 2306 42-43 0.4634133485926242 2306 44-45 0.291481171204957 2306 46-47 0.42000526389266923 2306 48-49 0.34968418738844065 2306 50-51 0.3285116285853178 2306 52-53 0.38295322462286663 2306 54-55 0.3546816505472563 2306 56-57 0.3555795181911918 2306 58-59 0.4759445273506273 2306 60-61 0.4167618564388462 2306 62-63 0.4590866536381597 2306 64-65 0.43673635923706655 2306 66-67 0.35450108721919094 2306 68-69 0.3208025543089832 2306 70-71 0.3379746951791276 2306 72-73 0.23575787902809253 2306 74-75 0.2944685717366724 2306 76-77 -0.1677808324724417 2306 78-79 0.27156098725357936 2306 80-81 0.29528566743483964 2306 82-83 0.4626927848511997 2306 84-85 0.43169776160380735 2306 86-87 0.44981817349395214 2306 88-89 0.6560645326206327 2306 90-91 0.4279254855364556 2306 92-93 0.43977155209631746 2306 94-95 0.3576532741217804 2306 96-97 0.3595840237146355 2306 98-99 0.3477471983243099 2306 100-101 0.2374580450766679 2307 1 0.1454186322627109 2307 2 0.12612898911626758 2307 3 0.125646634897862 2307 4 0.1621625327905889 2307 5 0.11803058222470497 2307 6 0.14766718206715268 2307 7 0.17148104609760395 2307 8 0.1712156570671155 2307 9 0.21473139728782087 2307 10-11 0.18015969848353564 2307 12-13 0.2268822516588358 2307 14-15 0.4203840175632436 2307 16-17 0.334034620156622 2307 18-19 0.3544477212052968 2307 20-21 0.3742280150082209 2307 22-23 0.37979911254286947 2307 24-25 0.3330439425861371 2307 26-27 0.33274989996335336 2307 28-29 0.36468566447128836 2307 30-31 0.35628024499096966 2307 32-33 0.37202940829374853 2307 34-35 0.37775111200050304 2307 36-37 0.2524741683622338 2307 38-39 0.3896297332790013 2307 40-41 0.36585996813828103 2307 42-43 0.3990444755160283 2307 44-45 0.4230831332385563 2307 46-47 0.44873408495839584 2307 48-49 0.38352926137606147 2307 50-51 0.2999555130264824 2307 52-53 0.31627913538977737 2307 54-55 0.18043499039263367 2307 56-57 0.24673925846101952 2307 58-59 0.3683599418883077 2307 60-61 0.34676775847650276 2307 62-63 0.39710166804378844 2307 64-65 0.4491548924906681 2307 66-67 0.3236640659564358 2307 68-69 0.19494630021432613 2307 70-71 0.09203062970387776 2307 72-73 0.11916697431122003 2307 74-75 0.1328230264639103 2307 76-77 -0.2422192413218056 2307 78-79 0.10690209948154461 2307 80-81 0.32157477701611725 2307 82-83 0.3720091974342452 2307 84-85 0.25636959581731844 2307 86-87 0.24569987590542297 2307 88-89 0.49805083953310714 2307 90-91 0.34942005614577454 2307 92-93 0.23355780041610785 2307 94-95 0.18940921360991325 2307 96-97 0.3295186060875146 2307 98-99 0.1108333686368077 2307 100-101 0.19593655176659652 2201 1 0.11246872062911706 2201 2 0.12006324646098676 2201 3 0.11660460463815525 2201 4 0.16289771528324337 2201 5 0.1675320041241477 2201 6 0.15357137291584877 2201 7 0.13524359579102452 2201 8 0.24246373813202382 2201 9 0.20089671755413008 2201 10-11 0.1540840191744266 2201 12-13 0.1652730495380368 2201 14-15 0.3628489246953066 2201 16-17 0.36910524427428015 2201 18-19 0.31161481691784587 2201 20-21 0.2909886496226832 2201 22-23 0.3380967461742159 2201 24-25 0.37315011216032445 2201 26-27 0.3442181298670022 2201 28-29 0.36045381895703343 2201 30-31 0.3857398257843343 2201 32-33 0.37769200206889053 2201 34-35 0.42056932237992584 2201 36-37 0.4093941466379718 2201 38-39 0.4596076437078551 2201 40-41 0.4488815227212726 2201 42-43 0.32959522104921035 2201 44-45 0.39416730822433266 2201 46-47 0.5200459749278181 2201 48-49 0.40261794682333374 2201 50-51 0.4289853173052549 2201 52-53 0.3973688875660599 2201 54-55 0.4041440575080699 2201 56-57 0.4833891935561567 2201 58-59 0.5447042924053918 2201 60-61 0.40883125401921205 2201 62-63 0.5564889239605293 2201 64-65 0.5620205861121335 2201 66-67 0.44745131115717385 2201 68-69 0.5259626234413446 2201 70-71 0.22447729528270344 2201 72-73 0.14215360447854408 2201 74-75 0.2757186842923147 2201 76-77 0.09635380972613206 2201 78-79 0.3229949325076049 2201 80-81 0.3662237184458732 2201 82-83 0.5040953345844201 2201 84-85 0.5148500508900895 2201 86-87 0.48591393610823275 2201 88-89 0.674433913774692 2201 90-91 0.3792208440964444 2201 92-93 0.5523340170676292 2201 94-95 0.5670751228502624 2201 96-97 0.4912641124712458 2201 98-99 0.4497436489332536 2201 100-101 0.30016791018998745 1101 1 0.14465048100263544 1101 2 0.09788719168723503 1101 3 0.12251055370587949 1101 4 0.12538753448977502 1101 5 0.14852713040954058 1101 6 0.14686010675180228 1101 7 0.1652184650195494 1101 8 0.1798314967024055 1101 9 0.11780750549310426 1101 10-11 0.10706421508120201 1101 12-13 0.19563663997179503 1101 14-15 0.2872897976956281 1101 16-17 0.2987214446932711 1101 18-19 0.23434575215710396 1101 20-21 0.21136722270009045 1101 22-23 0.24205451602513506 1101 24-25 0.2727023750186177 1101 26-27 0.3202620150755564 1101 28-29 0.2973640406914413 1101 30-31 0.3080350012859654 1101 32-33 0.41971047775911074 1101 34-35 0.29233683878896244 1101 36-37 0.28364664773771864 1101 38-39 0.33203740167719076 1101 40-41 0.32633917715357086 1101 42-43 0.3107260186572134 1101 44-45 0.2709374596622709 1101 46-47 0.42056339878419635 1101 48-49 0.28936179152648833 1101 50-51 0.3600547034959547 1101 52-53 0.2467136146772404 1101 54-55 0.28130271238026694 1101 56-57 0.38672588056263635 1101 58-59 0.40951097710349416 1101 60-61 0.3509324826831062 1101 62-63 0.3930960084861539 1101 64-65 0.39008756375576326 1101 66-67 0.33604947578331945 1101 68-69 0.3561472061268063 1101 70-71 0.16542934646759733 1101 72-73 0.23474873773260896 1101 74-75 0.09943786425390044 1101 76-77 -0.09865422602315377 1101 78-79 0.2008018915971519 1101 80-81 0.3446552447155895 1101 82-83 0.43994305169120906 1101 84-85 0.39203048286061914 1101 86-87 0.40094644931603796 1101 88-89 0.5948425392478001 1101 90-91 0.46782815510389497 1101 92-93 0.43905390696262003 1101 94-95 0.5292210816741303 1101 96-97 0.525212213290505 1101 98-99 0.4432873925906726 1101 100-101 0.24589175916145223 2304 1 0.13361282392563112 2304 2 0.13154876794332893 2304 3 0.10244104073372284 2304 4 0.11087192857449679 2304 5 0.11307099082540617 2304 6 0.10788998138156103 2304 7 0.09758094764322323 2304 8 0.08742624512233732 2304 9 0.1035928464294642 2304 10-11 0.1024702709752745 2304 12-13 0.15679091874596196 2304 14-15 0.2957573303224805 2304 16-17 0.21247946962316888 2304 18-19 0.18807124415729248 2304 20-21 0.24776722362017978 2304 22-23 0.22615532133590932 2304 24-25 0.1903939861538788 2304 26-27 0.15112528399476588 2304 28-29 0.18287561583576206 2304 30-31 0.22838165372186836 2304 32-33 0.18074986000915771 2304 34-35 0.25170920550400666 2304 36-37 0.23442746105329348 2304 38-39 0.23294384592687578 2304 40-41 0.25326468111494904 2304 42-43 0.28595655928043584 2304 44-45 0.301950511938081 2304 46-47 0.33651795235925164 2304 48-49 0.2835134549383227 2304 50-51 0.1669514852431675 2304 52-53 0.17255302637023107 2304 54-55 0.28195655296047306 2304 56-57 0.18334408136817615 2304 58-59 0.33283604494113206 2304 60-61 0.2057089500961169 2304 62-63 0.17563353866709974 2304 64-65 0.21309381961656015 2304 66-67 0.17435418274542513 2304 68-69 0.22431197471976105 2304 70-71 0.1520655810819278 2304 72-73 -0.04631971176620908 2304 74-75 0.030648698033857613 2304 76-77 -0.07191423566878896 2304 78-79 0.1397411172014813 2304 80-81 0.20928590050370133 2304 82-83 0.2179071481567476 2304 84-85 0.2564642772368586 2304 86-87 0.23469746620970255 2304 88-89 0.5732709756199625 2304 90-91 0.2806949939811716 2304 92-93 0.33484233787557827 2304 94-95 0.37277568186719634 2304 96-97 0.2061278507956068 2304 98-99 0.3765398728144369 2304 100-101 0.4088997171929769 2202 1 0.09886877592185783 2202 2 0.08736199365782937 2202 3 0.046648724369823924 2202 4 0.0772861097951747 2202 5 0.024273404034161672 2202 6 0.020347304373984798 2202 7 0.10299300522407151 2202 8 0.0709095631340304 2202 9 0.08155715762445936 2202 10-11 0.08393265530154537 2202 12-13 0.07912678615618773 2202 14-15 0.08102370521923774 2202 16-17 0.10553521311488367 2202 18-19 0.08866859438921892 2202 20-21 0.17815437287899982 2202 22-23 0.1651762770311791 2202 24-25 0.10865665704209704 2202 26-27 0.07211140167395058 2202 28-29 0.11700567835146813 2202 30-31 0.11762339758961105 2202 32-33 0.08935119805259717 2202 34-35 0.15321134239624 2202 36-37 0.1593370269494514 2202 38-39 0.16236635957802292 2202 40-41 0.2574887884337045 2202 42-43 0.29530760371722664 2202 44-45 0.26523505239503464 2202 46-47 0.30648683943154964 2202 48-49 0.1806262944420851 2202 50-51 0.21017515453504387 2202 52-53 0.19050616059508485 2202 54-55 0.12471498145150406 2202 56-57 0.20172454753974733 2202 58-59 0.31241381710795224 2202 60-61 0.2156460840952974 2202 62-63 0.17462833209930295 2202 64-65 0.20315261689645325 2202 66-67 0.210365753567757 2202 68-69 0.28645523508116355 2202 70-71 0.08144281568159073 2202 72-73 0.05339280016288939 2202 74-75 0.04869572283786283 2202 76-77 -0.22846147026379882 2202 78-79 0.0896428014039401 2202 80-81 0.25358692834785757 2202 82-83 0.3589384898628083 2202 84-85 0.3231021179844902 2202 86-87 0.32318234273525803 2202 88-89 0.5119106146647425 2202 90-91 0.288261691167115 2202 92-93 0.47356024361113924 2202 94-95 0.3728823792298428 2202 96-97 0.34731316452654326 2202 98-99 0.2568150663731821 2202 100-101 0.299017592300757 1102 1 0.0956059853677047 1102 2 0.04090755378283717 1102 3 0.059639152844276566 1102 4 0.07410916000360146 1102 5 0.08617357066623299 1102 6 -0.022033346108237595 1102 7 0.09698555447030088 1102 8 0.07196265536516222 1102 9 0.024844886883819584 1102 10-11 0.044927636702709606 1102 12-13 0.04464320530596666 1102 14-15 0.08678120787020305 1102 16-17 0.10991708949296708 1102 18-19 0.02421431441368327 1102 20-21 0.0349553597672454 1102 22-23 0.10112328859443664 1102 24-25 0.028367982412248693 1102 26-27 0.07099413926491138 1102 28-29 0.09603023554217316 1102 30-31 0.0914066889376457 1102 32-33 0.13013930602119217 1102 34-35 0.041589527060487796 1102 36-37 0.029745878646906476 1102 38-39 0.08151705500040407 1102 40-41 0.02821645721179067 1102 42-43 -0.00902903794527532 1102 44-45 -0.016654089590069532 1102 46-47 0.10594304100586527 1102 48-49 0.051046367154263805 1102 50-51 0.11571630199600946 1102 52-53 0.02870046088065692 1102 54-55 0.05353777077134225 1102 56-57 -0.06064879525664679 1102 58-59 0.06884121433747481 1102 60-61 0.030542401919312567 1102 62-63 0.05158773166280639 1102 64-65 0.07409678112721707 1102 66-67 0.14731157570344777 1102 68-69 0.11042774078985929 1102 70-71 -0.08157762900722787 1102 72-73 -0.1330797756771389 1102 74-75 -0.07396651900866402 1102 76-77 -0.060407839423962884 1102 78-79 0.04191617898547406 1102 80-81 0.09850135408436955 1102 82-83 0.15227741500181224 1102 84-85 0.23889583646044343 1102 86-87 0.2418046686271893 1102 88-89 0.4459583993559164 1102 90-91 0.10418781761673301 1102 92-93 0.2846234628080566 1102 94-95 0.3997776086154303 1102 96-97 0.18924939269465213 1102 98-99 0.12063718243213728 1102 100-101 0.19480978191346665 2305 1 0.07198894336242745 2305 2 0.06607365045103109 2305 3 0.044886409449865994 2305 4 -0.0032866365581867285 2305 5 0.03346313621479169 2305 6 -0.040583664763317984 2305 7 0.024447713783921188 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-0.6748128511660241 1213 90-91 -0.6857621965368637 1213 92-93 -0.875303478081392 1213 94-95 -0.7993996151383627 1213 96-97 -0.8487764071079411 1213 98-99 -0.9636268742453318 1213 100-101 -0.7080567671247984 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 25.0 10 31.0 11 39.0 12 87.0 13 225.0 14 638.0 15 1506.0 16 2897.0 17 4914.0 18 7258.0 19 9717.0 20 12920.0 21 16708.0 22 21194.0 23 27041.0 24 34104.0 25 42920.0 26 55677.0 27 76412.0 28 115875.0 29 154941.0 30 132937.0 31 131093.0 32 171151.0 33 235307.0 34 349798.0 35 570564.0 36 1029871.0 37 1791913.0 38 885529.0 39 5340.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.04845714565653 27.222817955366736 19.23240351334378 16.49632138563296 2 29.51249507950735 27.43839916802493 28.184615865332525 14.864489887135191 3 33.67204308105412 21.778307688676176 27.498781123797915 17.050868106471786 4 29.24761307358465 22.336240057764208 28.02369066187812 20.392456206773026 5 32.88259526226608 20.567472478355775 29.29303856002016 17.256893699357985 6 30.235310779844166 21.363378085051245 30.8416309509941 17.559680184110483 7 30.871739780913686 24.912670807748754 25.030372048680867 19.185217362656694 8 28.49163083212111 30.398964921939502 22.785523579476273 18.32388066646311 9 30.732607438256522 25.381811549631685 24.718788962744863 19.166792049366936 10-11 26.034481528892595 29.856144123851468 27.866283032882848 16.24309131437309 12-13 24.110872497171673 29.512461115796217 27.230906365784413 19.1457600212477 14-15 23.327626863801104 29.781835799721428 27.756987269382154 19.13355006709531 16-17 24.698668173315184 27.90089914073315 27.73596086585759 19.664471820094075 18-19 22.015141701697406 28.312667547232483 29.87773233810603 19.794458412964083 20-21 23.622389421798385 28.093932756606876 30.062418508320597 18.22125931327414 22-23 21.640242329020005 32.36884352330955 27.628663526534424 18.362250621136024 24-25 21.21739356367296 28.428611166113456 30.076428539163047 20.27756673105054 26-27 21.38883388654558 28.192631301159963 27.92468309310234 22.49385171919211 28-29 23.021690244840997 30.659585459320134 27.776015438544533 18.542708857294336 30-31 20.917867595189243 28.448038408881647 30.030169964599622 20.603924031329484 32-33 20.98250053747573 30.60904745715393 27.725681218645125 20.682770786725214 34-35 22.616265425043956 30.548948670303727 28.21404542102945 18.620740483622868 36-37 20.86814825205298 30.220556587543733 29.935156687618804 18.97613847278448 38-39 20.74027700674847 28.737000502496947 29.819568103959636 20.703154386794946 40-41 22.52133158909321 28.355996510387577 30.0738086653727 19.04886323514652 42-43 20.681101869445992 30.33301411972531 29.767608644003353 19.218275366825345 44-45 20.364972602048816 28.35334297119899 30.256926161642735 21.02475826510946 46-47 22.515113049507832 28.192497705010627 27.963291718262003 21.329097527219538 48-49 20.37280416279555 30.527999543490548 29.83059134702301 19.268604946690886 50-51 22.310600608747745 28.210053211295076 30.033307477050958 19.446038702906222 52-53 20.523339850789952 28.384798654404324 27.80344107104905 23.288420423756666 54-55 22.12513549856466 28.340045457035117 30.208462922964635 19.326356121435587 56-57 20.42228713201405 28.15923839831833 29.901790016691333 21.516684452976286 58-59 20.280384094734966 28.667999321584265 29.876892342947137 21.17472424073363 60-61 22.075043752579955 28.24540371812647 30.162169662607717 19.517382866685853 62-63 20.236227525970722 28.472918659393294 29.781463428716748 21.50939038591924 64-65 21.96242380391897 28.445158353672102 30.14367069365821 19.448747148750716 66-67 20.304748107406926 31.909118838630295 27.93828116950688 19.847851884455906 68-69 20.17742550928644 32.359416339408625 27.8533149064995 19.609843244805436 70-71 20.075675498447747 32.017763848989105 28.153753803544905 19.75280684901824 72-73 20.124265817039298 32.12137936704663 27.847507039459025 19.90684777645505 74-75 19.970150387045283 31.79061920025562 28.311173891407172 19.92805652129193 76-77 20.276299822486806 31.344907508473458 28.044043794402718 20.334748874637018 78-79 20.11610833267517 31.55621003786769 28.14469550861513 20.182986120842006 80-81 20.104081887760994 31.01874465850434 28.410447320114727 20.466726133619932 82-83 20.24997548219413 31.171815264382612 27.969165728055717 20.609043525367543 84-85 20.06946672375454 30.884779362224755 28.501304093292134 20.54444982072857 86-87 20.332579767142697 30.50515432310299 28.31514132996747 20.847124579786847 88-89 20.245514857789956 30.97605506555289 28.213172134376173 20.565257942280976 90-91 20.329558006005474 30.396963544614923 28.458913111700063 20.81456533767954 92-93 20.366111885019368 30.52721344863495 28.134496203281323 20.97217846306436 94-95 20.117052004465467 30.48380181540341 28.519968554206958 20.87917762592416 96-97 20.32292493186967 30.266530023803785 28.244286998254932 21.16625804607161 98-99 20.206317864533315 30.56001825208432 28.205968248100593 21.027695635281766 100-101 20.386163584076623 30.19195315041753 28.275461929916023 21.146421335589825 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 22.0 1 18.5 2 20.5 3 39.5 4 49.5 5 35.5 6 30.0 7 39.5 8 45.0 9 56.5 10 64.0 11 77.5 12 101.0 13 129.0 14 164.5 15 229.0 16 366.5 17 575.0 18 921.5 19 1550.5 20 2520.0 21 4042.5 22 6410.5 23 9833.5 24 14532.0 25 20517.5 26 27945.0 27 37097.0 28 48161.5 29 60772.0 30 74785.5 31 91132.5 32 110252.5 33 132432.0 34 156962.0 35 184582.5 36 217861.0 37 253676.0 38 286804.0 39 312087.0 40 328151.5 41 333158.5 42 325069.5 43 309994.0 44 291583.0 45 272943.0 46 256600.5 47 240120.5 48 219862.5 49 198520.5 50 178458.5 51 157809.0 52 136648.5 53 115542.0 54 95920.0 55 78873.5 56 64637.0 57 52340.0 58 41891.5 59 33397.0 60 26052.5 61 19798.5 62 14859.0 63 10951.5 64 7960.5 65 5788.5 66 4144.5 67 2906.5 68 2084.5 69 1479.0 70 1070.5 71 775.0 72 561.0 73 420.0 74 312.5 75 232.0 76 183.5 77 148.0 78 111.0 79 81.0 80 55.0 81 44.0 82 39.5 83 34.5 84 26.0 85 21.0 86 17.0 87 11.0 88 8.0 89 3.5 90 1.0 91 2.0 92 2.0 93 1.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16632029341929322 2 0.0 3 2.8869154463222223E-4 4 0.021312228736084644 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.113468003976472E-4 12-13 0.0 14-15 0.0 16-17 0.0012142026730119937 18-19 0.0 20-21 0.0 22-23 5.519103059145426E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.934385506461766E-4 38-39 7.641835004970588E-4 40-41 0.005179465947813399 42-43 0.0035492078134196736 44-45 0.0010019294784294774 46-47 0.004525664508499249 48-49 0.008142799744185327 50-51 0.005442684709095719 52-53 0.0015878034954772224 54-55 0.011216515601740164 56-57 0.019546115757158106 58-59 0.023647233876492323 60-61 0.011878807968837616 62-63 6.028558726143465E-4 64-65 0.014740250631809936 66-67 0.035177913806214614 68-69 0.020539554307804284 70-71 0.03716479090750696 72-73 0.030940940842347584 74-75 0.03047393981426605 76-77 0.03931299563668203 78-79 0.017542256800299152 80-81 0.013143956208549412 82-83 0.0010274022617793792 84-85 0.010851405707058236 86-87 0.0220509394532318 88-89 0.021312228736084644 90-91 0.025651092833351277 92-93 0.04361789602281547 94-95 0.0285634810630234 96-97 0.029871083941651704 98-99 0.037139318124157064 100-101 0.01748282030581605 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5888638.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.02515362699498 #Duplication Level Percentage of deduplicated Percentage of total 1 97.13142311869156 85.50008442031476 2 1.921293880059345 3.3824437790965827 3 0.37859893134610295 0.9997868728427052 4 0.15405651458307168 0.5424339345365711 5 0.09318400801068219 0.41012683103597153 6 0.057138138627814895 0.3017756058404365 7 0.050970327385600964 0.3140669628975027 8 0.04291378789898801 0.3021994218019758 9 0.01984962532770269 0.1572539687018388 >10 0.1270307483839207 2.1770567491740875 >50 0.01383856496720166 0.9002175338115571 >100 0.00927840896340997 1.3136744476450162 >500 2.671366301165407E-4 0.14659599418706531 >1k 1.3761092496450511E-4 0.1712854128978567 >5k 0.0 0.0 >10k+ 1.919819947663187E-5 3.3809980652160685 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 200068 3.397525879498791 TruSeq Adapter, Index 7 (97% over 35bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0013585484453281047 0.0 0.0 2 0.0016642218455269282 0.0 0.0 3 0.001918949679025948 0.0 0.0 4 0.002020840812425556 0.0 0.0 5 0.002309532357057778 0.0 5.0945566699803926E-5 6 0.002751060601789412 0.0 6.792742226640523E-5 7 0.0035492078134196736 0.0 8.490927783300654E-5 8 0.0042964094583501315 0.0 8.490927783300654E-5 9 0.004890774403181177 0.0 8.490927783300654E-5 10-11 0.006656887382107714 0.0 8.490927783300654E-5 12-13 0.008363563866551144 0.0 9.34002056163072E-5 14-15 0.010460823029026407 0.0 1.103820611829085E-4 16-17 0.012481663841451962 0.0 1.2736391674950982E-4 18-19 0.015020451248658858 0.0 1.3585484453281047E-4 20-21 0.01781396648936477 0.0 1.3585484453281047E-4 22-23 0.021218828530468338 0.0 1.528367000994118E-4 24-25 0.024428399232555985 0.0 1.6132762788271244E-4 26-27 0.027952134262625754 8.490927783300654E-6 1.7830948344931376E-4 28-29 0.03168814248727804 1.698185556660131E-5 1.9529133901591506E-4 30-31 0.04783788713111589 1.698185556660131E-5 2.037822667992157E-4 32-33 0.05333151740691141 1.698185556660131E-5 2.037822667992157E-4 34-35 0.058001527687726775 3.396371113320262E-5 2.2076412236581703E-4 36-37 0.06331684848007299 3.396371113320262E-5 2.2925505014911767E-4 38-39 0.06909067937271743 3.396371113320262E-5 2.5472783349901964E-4 40-41 0.07544189335462631 3.396371113320262E-5 2.5472783349901964E-4 42-43 0.0829393825872808 3.396371113320262E-5 2.7170968906562094E-4 44-45 0.09086990913688361 3.396371113320262E-5 2.7170968906562094E-4 46-47 0.09940329155910077 3.396371113320262E-5 2.7170968906562094E-4 48-49 0.10853952985393227 3.396371113320262E-5 2.9718247241552294E-4 50-51 0.11818522381576181 3.396371113320262E-5 3.1416432798212423E-4 52-53 0.1284762282891222 3.396371113320262E-5 3.226552557654249E-4 54-55 0.13927668842948063 3.396371113320262E-5 3.481280391153268E-4 56-57 0.15069698629802003 3.396371113320262E-5 3.5661896689862747E-4 58-59 0.16433341631800086 3.396371113320262E-5 3.5661896689862747E-4 60-61 0.1779273916990652 3.396371113320262E-5 3.905826780318301E-4 62-63 0.1919459134692946 3.396371113320262E-5 4.075645335984314E-4 64-65 0.20800225790751614 3.396371113320262E-5 4.075645335984314E-4 66-67 0.2256464058412149 3.396371113320262E-5 4.075645335984314E-4 68-69 0.24458117479797536 3.396371113320262E-5 4.075645335984314E-4 70-71 0.26625002250095864 3.396371113320262E-5 4.330373169483334E-4 72-73 0.291111459050463 3.396371113320262E-5 4.5851010029823535E-4 74-75 0.3177050448677606 3.396371113320262E-5 4.7549195586483664E-4 76-77 0.34799218427079404 3.396371113320262E-5 4.7549195586483664E-4 78-79 0.38081811108103436 3.396371113320262E-5 4.92473811431438E-4 80-81 0.4188999221891378 3.396371113320262E-5 5.009647392147387E-4 82-83 0.46204232625608843 3.396371113320262E-5 5.349284503479413E-4 84-85 0.5105340148265185 3.396371113320262E-5 5.434193781312419E-4 86-87 0.5645872610950105 3.396371113320262E-5 5.434193781312419E-4 88-89 0.6253738130956599 3.396371113320262E-5 5.604012336978432E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 27960 0.0 76.7533 3 CGGAAGA 28045 0.0 76.23275 4 GATCGGA 28390 0.0 75.7484 1 ATCGGAA 28490 0.0 75.34213 2 GAAGAGC 30685 0.0 69.84733 6 GAGCACA 31760 0.0 67.01962 9 AGAGCAC 31825 0.0 66.98719 8 AAGAGCA 35030 0.0 61.034576 7 GGAAGAG 37575 0.0 57.267162 5 GCCGTCT 23740 0.0 44.84154 50-51 ATGCCGT 23670 0.0 44.8058 48-49 TGCCGTC 23700 0.0 44.646637 50-51 TATGCCG 23705 0.0 44.629448 48-49 TCTCGTA 23690 0.0 44.55652 42-43 CGTATGC 23785 0.0 44.458237 46-47 CGTCTTC 23905 0.0 44.424057 52-53 CTCGTAT 23840 0.0 44.20626 44-45 TATCTCG 23710 0.0 44.107365 40-41 CCGTCTT 23955 0.0 44.083447 52-53 TCGTATG 24190 0.0 44.02803 44-45 >>END_MODULE