FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_35008-2.341000000358b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_35008-2.341000000358b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5888638
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2000683.397525879498791TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG279600.076.75333
CGGAAGA280450.076.232754
GATCGGA283900.075.74841
ATCGGAA284900.075.342132
GAAGAGC306850.069.847336
GAGCACA317600.067.019629
AGAGCAC318250.066.987198
AAGAGCA350300.061.0345767
GGAAGAG375750.057.2671625
GCCGTCT237400.044.8415450-51
ATGCCGT236700.044.805848-49
TGCCGTC237000.044.64663750-51
TATGCCG237050.044.62944848-49
TCTCGTA236900.044.5565242-43
CGTATGC237850.044.45823746-47
CGTCTTC239050.044.42405752-53
CTCGTAT238400.044.2062644-45
TATCTCG237100.044.10736540-41
CCGTCTT239550.044.08344752-53
TCGTATG241900.044.0280344-45
Produced by FastQC (version 0.11.2)