FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_35008-1.341000000357b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_35008-1.341000000357b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2434739
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT332841.3670459133401978TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC50150.20597690347918196No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG40760.16741014129235207No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA39780.16338506920043586No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC38400.15771711054039056No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA32560.13373096664570616No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA31430.12908981209074155No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG29860.12264148231083496No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC27450.11274309073785733No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACG156.577498E-495.14981
CGCGTGG156.577498E-495.14981
TCGGAAG52200.068.4244163
CGGAAGA52150.068.398944
GCCGAAC353.3863344E-667.964141
GATCGGA53950.066.313861
ATCGGAA55450.064.413962
GAAGAGC56000.063.6064766
GGAAGAG61650.058.1623545
CGTCCCG250.00502331656.996084
TTGTGTT270300.049.1765751
CGATCGG502.8017217E-547.57491
CGACGGG751.3468525E-744.403241
CGAGTCC554.919002E-543.2499121
GCCGTCT41400.043.1980450-51
TCGCCGG554.9669696E-543.178852
ATGCCGT41550.042.75453648-49
TCGTATG41550.042.69649544-45
TATGCCG41100.042.64480648-49
TGCCGTC41300.042.61255650-51
Produced by FastQC (version 0.11.2)